CN108201136A - Chromium-rich nutrition fortifier and preparation method thereof - Google Patents

Chromium-rich nutrition fortifier and preparation method thereof Download PDF

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CN108201136A
CN108201136A CN201711415130.1A CN201711415130A CN108201136A CN 108201136 A CN108201136 A CN 108201136A CN 201711415130 A CN201711415130 A CN 201711415130A CN 108201136 A CN108201136 A CN 108201136A
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chromium
enzymolysis
preparation
nutrition fortifier
rich
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成玉梁
张燕
郭亚辉
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Institute Of Food Biotechnology Jiangnan University (rugao)
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Institute Of Food Biotechnology Jiangnan University (rugao)
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/16—Inorganic salts, minerals or trace elements
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/06—Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/40—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by drying or kilning; Subsequent reconstitution
    • A23L3/46—Spray-drying
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/15—Vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/18—Peptides; Protein hydrolysates
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/185—Vegetable proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A23L33/19—Dairy proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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Abstract

The present invention relates to a kind of preparation method of chromium-rich nutrition fortifier, step is as follows（One）, extraction natural organic chromium masterbatch, including natural material is cleaned, again natural organic chromium extract is made through series of processes such as crushing, superfine grinding, enzymolysis, enzyme-deactivating, centrifugation, nanofiltration, concentrate dryings.（Two）, compounding：By natural organic selenium extract and antioxidant, vitamin, protein powder, minerals in mass ratio（1.2‑1.5）:（0.8‑1.2）:（0.2‑0.5）:（0.2‑0.5）:（0.6‑1.2）Ratio is uniformly mixed, and is sieved with 100 120 mesh sieve, is obtained the chromium-rich antioxidant after being compounded.Chromium-rich nutrition fortifier prepared by the present invention is safe and non-toxic, stability is high, has the effect of a variety of diseases such as anti-oxidant, prevention cholesterol, cardiovascular and cerebrovascular.

Description

Chromium-rich nutrition fortifier and preparation method thereof
Technical field
The present invention relates to a kind of preparation method of nutrition fortifier, more particularly to a kind of preparation side of chromium-rich nutrition fortifier Method.
Background technology
Chromium is the essential trace elements of the human body, participates in the glycometabolism of human body and lipid metaboli process, promotes fat and protein Synthesis, and then promote growth in humans development.Human body is extremely low to the absorption rate of inorganic chromium, and less than 1%, human body is to Organic Chromium Utilization rate up to 10-25%.Research shows that：When human body lacks chromium, easily cause diabetes, angiocardiopathy, hyperlipidemia and courage Sterol etc., in addition with research shows that, it is related that myopia also lacks chromium with human body, and when human body lacks chromium, insulin action reduction makes Obstacle occurs for the utilization of sugar so that cholesterol level increases, and the synthesis for artery sclerosis-diabetes occur lacks chromium disease.If occur high When blood glucose, glycosuria, vascular sclerosis, blood glucose, which increases, causes osmotic pressure to reduce, and eye lens and aqueous humor osmotic pressure is caused to change, is promoted Crystalline lens is made to become convex, diopter increases, and forms myopia.Chromium content is higher in body when in general, below children 10 years old, but 10 arrive Internal chromium can reduce suddenly within 30 years old, therefore myopia most easily occurs for this stage, it should be noted that absorb containing the high food of chromium.
The toxicity such as general inorganic chromium such as chromate and bichromate are larger, inedibility, therefore are often added in food Machine chromium is to meet the needs of human body is to chromium.Organic Chromium is typically found in wheat, flour, animal's liver etc., but food is by processing Chromium content can substantially reduce, in addition, organic chromium content in plant also has very big relationship with the soil of growth, water quality, together The content that the soil difference of a kind of plant but growth often leads to its inside chromium is different, and source is very unstable.Therefore it is current Research has mainly also concentrated on the high-purity extraction for how realizing Organic Chromium and production chromium content higher, harmless organic Crop or food additives.National inventing patent CN 102406186A disclose a kind of Organic Chromium dried whole-egg and its producer By the way that egg white is detached with yolk, egg-white powder, the yolk powder of low cholesterol is made in method, and be separately added into selenium-rich, it is chromium-rich again It is mixed and stirs evenly, organic selenium chromium whole egg powder is made under certain technique.National inventing patent CN101372674A is disclosed A kind of production method of Cr-enriched yeast, by the way that fermented glutinous rice yeast Spawn incubation several times, is fermented under certain condition, it It adds in when the fermentation of divalent chromic salts reaches 110-150g/L to weight in wet base and stops afterwards, produced by centrifuging, being dried to obtain Cr-enriched yeast Product.
The above-mentioned method for preparing chromium-rich product, production technology are complicated, and be all by inorganic chromium be converted to Organic Chromium or It is chromium-rich to achieve the purpose that be directly added into inorganic chromate salt, has certain side effect to human body, and chromium-rich using barms progress The cultivation of yeast, production technology is complicated, and the period of fermentation or mutation is long, and bivalent chromium molecules of salt activity is high, inoxidizability It can be poor, it is easy to be aoxidized.Therefore, our a kind of chromium-rich nutrition fortifiers newly developed, directly from enriched-Cr malt, buckwheat or Cassia Organic Chromium is extracted in the natural materials such as son, is the small molecule chromium for being easier to absorb by its chemical breakdown, separately adds in other plant battalion The substances such as element, amino acid and other nitrogenous compounds are supported, obtain a kind of chromium-rich battalion pollution-free, chromium content is high, nontoxic Hardening agent is supported, had both met needs of the human body to chromium, but also with certain anti-oxidation function.
Invention content
Toxicity is carried to solve inorganic chromium in the prior art, is not suitable for being added in food, and it is difficult from natural material To obtain the Organic Chromium of high-purity, and Organic Chromium activity is high, easy oxygenolysis, it is difficult to meet human body to Trace Elements in Food The demand of chromium.The present invention provide it is a kind of prepare that safe and non-toxic, stability is high, there is anti-oxidant, prevention cholesterol, cardiovascular and cerebrovascular etc. The method of the chromium-rich nutrition fortifier of a variety of diseases.
The technical solution adopted by the present invention to solve the technical problems is：
First, natural organic chromium masterbatch is extracted
（1）, cleaning：The natural material of 150-200g is cleaned 2-3 times with 30-40 DEG C of water and is dried；The natural material is Enriched-Cr malt, buckwheat or cassia seed.
（2）, crush：Clean natural material with pulverizer is crushed, is sieved with 100 mesh sieve.
（3）, superfine grinding：Superfine grinding, milling time 10-30min, work(are carried out to the particle after crushing using ball milling instrument Rate 10-30HZ obtains enzymolysis raw material, is put at 0-3 DEG C and carries out preservation；Add in appropriate protection liquid in ball milling, the protection liquid by The Raw material processing of following parts by weight forms：1-3 parts of reduced glutathione, 3-6 parts of xanthans, 5-10 parts of avenabeta glucosan, 1-3 parts of bee glue powder, 0.5-1 parts of polylysine, 60-80 parts of deionized water.
（4）, enzymolysis：By step（3）In enzymolysis raw material and water 1:5（Weight）Mixing, and stir evenly, it adjusts pH value and arrives 5.6-6.7 or so adds in the complex enzyme of 0.8%-1.2% and is stirred continuously, enzymolysis time 2-4h, 45-55 DEG C of hydrolysis temperature；It is described Complex enzyme include protease, cellulase, amylase by weight（0.8-1.2）:（0.8-1.2）:（0.5-1.2）Composition.
（5）, enzyme-deactivating：After enzymolysis, enzymolysis liquid is cooled to 30-50 DEG C in 110-120 DEG C, heating 3-5s enzyme deactivations；
（6）, centrifuge：Organic Chromium extracting solution after inactivation in centrifuge is centrifuged, obtains Organic Chromium clear liquid, Centrifuge speed 4000-6000rmp, centrifugation time 5-10min.
（7）, nanofiltration：Organic Chromium clear liquid is subjected to dialysis concentration with nanometer film, the nanometer retaining molecular weight is 150-500Da, the Organic Chromium extracting solution after being concentrated.
（8）, be concentrated and dried：By the Organic Chromium extracting solution after concentration in 60-80 DEG C of temperature, vacuum degree -0.075- Solid content is further concentrated under 0.095mpa as 30-40%, moisture content is then dried to obtain in spray dryer again is The powdered Organic Chromium extract of 4%-7%.
Above-mentioned steps（3）The preparation method step of middle oxidized form of glutathione is as follows：
（1）Reduced glutathione is taken, in 40-45 DEG C of dissolving, the reduced glutathione solution of 50-100g/L is obtained, surveys it PH value is A1.
（2）With barium hydroxide tune pH to A2,7.0≤A2≤7.2.
（3）A certain amount of hydrogen peroxide is added in, it is stirring while adding, continue to be stirred to react 0.2-0.5h after adding.
（4）After reaction, a certain amount of manganese dioxide is added in, stirs 5-10min, then fully filters off manganese dioxide.
（5）With sulphur acid for adjusting pH to A3, A1-0.1≤A3≤A1.
（6）Barium sulfate precipitate is fully filtered off, spray drying obtains oxidized form of glutathione.
2nd, it compounds
By natural organic chromium extract and nutrition fortifier, vitamin, protein powder, minerals in mass ratio（1.2-1.5）: （0.8-1.2）:（0.2-0.5）:（0.2-0.5）:（0.6-1.2）It is uniformly mixed, is sieved, is obtained with 100-120 mesh sieve Chromium-rich nutrition fortifier after compounding.
The nutrition fortifier is natural VE, modified glucan, xylitol or maltitol.
The vitamin is VB1（Thiamine）、VB2（Riboflavin）, VB11 folic acid or vitamin C.
The protein powder is：Casein, lactalbumin or pea protein.
The minerals are：Calcium chloride, potassium chloride or sodium chloride.
The beneficial effects of the invention are as follows：
1st, the raw materials particles after crushing are further ground using ball milling instrument so that its can more fully by enzymatic hydrolysis, Reduce the time of enzymatic so that the Organic Chromium of macromolecular is easier to be broken down into small molecule Organic Chromium, is more advantageous to by people Body absorbs.
2nd, it is centrifuged using centrifuge, can obtain the purer Organic Chromium extracting solution of purity, last concentrated drying The powdered Organic Chromium obtained afterwards easily preserves, and is unlikely to deteriorate.
3rd, chromium-rich antioxygen is made by adding in antioxidant, vitamin, protein powder, minerals in Organic Chromium extract Agent the multi-efficiencies such as has both anti-oxidant, whitening, replenishes the calcium, gives protection against cancer, being readily produced, without side-effects to human body.
Specific embodiment
Below by specific embodiment, technical scheme of the present invention is described in further detail.
Embodiment 1：
First, natural organic chromium masterbatch is extracted
（1）, cleaning：150 enriched-Cr malt is cleaned 2-3 times with 30-40 DEG C of water and is dried.
（2）, crush：Enriched-Cr malt after drying with pulverizer is crushed, is sieved with 100 mesh sieve.
（3）, superfine grinding：Superfine grinding, milling time are carried out to the enriched-Cr malt particle after crushing using ball milling instrument 10min, power 10HZ obtain enzymolysis raw material, are put at 3 DEG C and carry out preservation；Appropriate protection liquid, the protection liquid are added in ball milling It is formed by the Raw material processing of following parts by weight：1 part of reduced glutathione, 3 parts of xanthans, 5 parts of avenabeta glucosan, bee glue powder 1 part, 0.5 part of polylysine, 60 parts of deionized water.
（4）, enzymolysis：By step（3）In enzymolysis raw material and water 1:5（Weight）Mixing, and stir evenly, it adjusts pH value and arrives 5.6 or so, it is 0.8 to add in 0.8% weight ratio:1.2 cellulase, the complex enzyme of amylase are simultaneously stirred continuously, enzymolysis time 2h, 45 DEG C of hydrolysis temperature.
（5）, enzyme-deactivating：After enzymolysis, enzymolysis liquid is cooled to 35 DEG C in 110 DEG C, heating 3s enzyme deactivations.
（6）, centrifuge：Organic Chromium extracting solution after inactivation in centrifuge is centrifuged, obtains Organic Chromium Clear liquid, centrifuge speed 4000rmp, centrifugation time 5min.
（7）, nanofiltration：Organic Chromium clear liquid is subjected to dialysis concentration with nanometer film, the nanometer retaining molecular weight is 150Da, the Organic Chromium extracting solution after being concentrated.
（8）, be concentrated and dried：By the Organic Chromium extracting solution after concentration under temperature 60 C, vacuum degree -0.075mpa further It is 30% to be concentrated into solid content, is then dried to obtain the powdered Organic Chromium that moisture content is 4% in spray dryer again and extracts Object.
Above-mentioned steps（3）The preparation method step of middle oxidized form of glutathione is as follows：
（1）Reduced glutathione is taken, in 40 DEG C of dissolvings, obtains the reduced glutathione solution of 50g/L, surveying its pH value is A1。
（2）With barium hydroxide tune pH to A2=7.0.
（3）A certain amount of hydrogen peroxide is added in, it is stirring while adding, continue to be stirred to react 0.2h after adding.
（4）After reaction, a certain amount of manganese dioxide is added in, stirs 5min, then fully filters off manganese dioxide.
（5）With sulphur acid for adjusting pH to A3=A1-0.1.
（6）Barium sulfate precipitate is fully filtered off, spray drying obtains oxidized form of glutathione.
2nd, it compounds
By natural organic chromium extract and natural VE, VB1（Thiamine）, lactalbumin, calcium chloride press 1.2:0.8:0.8:0.2: 0.2 ratio is uniformly mixed, and is sieved with 100-120 mesh sieve, is obtained the chromium-rich nutrition fortifier after being compounded.
Embodiment two：
First, natural organic chromium masterbatch is extracted
（1）, cleaning：The buckwheat of 180g is cleaned 2-3 times with 30-40 DEG C of water and is dried.
（2）, crush：Buckwheat after drying with pulverizer is crushed, is sieved with 100 mesh sieve.
（3）, superfine grinding：Using ball milling instrument to after crushing buckwheat particle carry out superfine grinding, milling time 20min, Power 20HZ obtains enzymolysis raw material, is put at 0-3 DEG C and carries out preservation；Appropriate protection liquid is added in ball milling, the protection liquid is under The Raw material processing of row parts by weight forms：2 parts of reduced glutathione, 4 parts of xanthans, 7 parts of avenabeta glucosan, 2 parts of bee glue powder, 0.7 part of polylysine, 80 parts of deionized water.
（4）, enzymolysis：By step（3）In enzymolysis raw material and water 1:5（Weight）Mixing, and stir evenly, it adjusts pH value and arrives 6 or so, add in 1% amylase and be stirred continuously, enzymolysis time 3h, 50 DEG C of hydrolysis temperature；The complex enzyme includes albumen Enzyme, cellulase, amylase are by weight 1:1:0.8 composition.
（5）, enzyme-deactivating：After enzymolysis, enzymolysis liquid is cooled to 40 DEG C in 115 DEG C, heating 4s enzyme deactivations；
（6）, centrifuge：Organic Chromium extracting solution after inactivation in centrifuge is centrifuged, obtains Organic Chromium clear liquid, Centrifuge speed 5000rmp, centrifugation time 7min.
（7）, nanofiltration：Organic Chromium clear liquid is subjected to dialysis concentration with nanometer film, the nanometer retaining molecular weight is 320Da, to concentration after Organic Chromium extracting solution.
（8）, be concentrated and dried：Organic Chromium extracting solution after concentration is further dense under temperature 70 C, vacuum degree 0.01mpa It is 35% to be reduced to solid content, is then dried to obtain the powdered Organic Chromium that moisture content is 5.5% in spray dryer again and extracts Object.
Above-mentioned steps（3）The preparation method step of middle oxidized form of glutathione is as follows：
（1）Reduced glutathione is taken, in 42 DEG C of dissolvings, obtains the reduced glutathione solution of 75g/L, surveying its pH value is A1。
（2）With barium hydroxide tune pH to A2=7.1.
（3）A certain amount of hydrogen peroxide is added in, it is stirring while adding, continue to be stirred to react 0.35h after adding.
（4）After reaction, a certain amount of manganese dioxide is added in, stirs 7.5min, then fully filters off manganese dioxide.
（5）With sulphur acid for adjusting pH to A3=A1-0.05.
（6）Barium sulfate precipitate is fully filtered off, spray drying obtains oxidized form of glutathione.
2nd, it compounds
By natural organic chromium extract and modified glucan, VB1（Thiamine）, VB11 folic acid, lactalbumin, sodium chloride press 1.3: 1:1:0.35:0.35 is uniformly mixed, and is sieved with 100-120 mesh sieve, the chromium-rich nutrition fortifier after being compounded.
Embodiment 3：
First, natural organic chromium masterbatch is extracted
（1）, cleaning：The cassia seed of 200g is cleaned 2-3 times with 30-40 DEG C of water and is dried.
（2）, crush：Cassia seed after drying with pulverizer is crushed, is sieved with 100 mesh sieve.
（3）, superfine grinding：Superfine grinding, milling time 30min, power are carried out to the particle after crushing using ball milling instrument 30HZ obtains enzymolysis raw material, is put at 0-3 DEG C and carries out preservation；Appropriate protection liquid is added in ball milling, the protection liquid is by following heavy The Raw material processing of amount part forms：3 parts of reduced glutathione, 6 parts of xanthans, 10 parts of avenabeta glucosan, 3 parts of bee glue powder gather 1 part of lysine, 70 parts of deionized water.
（4）, enzymolysis：By step（3）In enzymolysis raw material and water 1:5（Weight）Mixing, and stir evenly, it adjusts pH value and arrives 6.7 or so, add in 1.2% complex enzyme and be stirred continuously, enzymolysis time 4h, 55 DEG C of hydrolysis temperature；The complex enzyme includes egg White enzyme, cellulase, amylase are by weight 1.2:1:1.2 composition.
（5）, enzyme-deactivating：After enzymolysis, enzymolysis liquid is cooled to 50 DEG C in 120 DEG C, heating 5s enzyme deactivations；
（6）, centrifuge：Organic Chromium extracting solution after inactivation in centrifuge is centrifuged, obtains Organic Chromium clear liquid, Centrifuge speed 6000rmp, centrifugation time 10min.
（7）, nanofiltration：Organic Chromium clear liquid is subjected to dialysis concentration with nanometer film, the nanometer retaining molecular weight is 500Da, the Organic Chromium extracting solution after being concentrated.
（8）, be concentrated and dried：By the Organic Chromium extracting solution after concentration under 80 DEG C of temperature, vacuum degree 0.095mpa further It is 40% to be concentrated into solid content, is then dried to obtain the powdered Organic Chromium that moisture content is 7% in spray dryer again and extracts Object.
Above-mentioned steps（3）The preparation method step of middle oxidized form of glutathione is as follows：
（1）Reduced glutathione is taken, in 45 DEG C of dissolvings, obtains the reduced glutathione solution of 100g/L, surveying its pH value is A1。
（2）With barium hydroxide tune pH to A2=7.2.
（3）A certain amount of hydrogen peroxide is added in, it is stirring while adding, continue to be stirred to react 0.5h after adding.
（4）After reaction, a certain amount of manganese dioxide is added in, stirs 10min, then fully filters off manganese dioxide.
（5）With sulphur acid for adjusting pH to A3=A1.
（6）Barium sulfate precipitate is fully filtered off, spray drying obtains oxidized form of glutathione.
2nd, it compounds
Natural organic chromium extract and modified glucan, vitamin C, lactalbumin, potassium chloride are pressed 1.5:1.2:1.2:0.5: 0.5 ratio is uniformly mixed, and is sieved with 100-120 mesh sieve, is obtained the chromium-rich nutrition fortifier after being compounded.
The chromium-rich nutrition fortifier performance evaluation that embodiment 1-3 is prepared：
The chromium-rich nutrition fortifier that ordinary nutritional hardening agent and embodiment are obtained carries out nutrient analysis, conclusion such as following table It is shown：
The experimental results showed that the content of every nutrient is without departing from the limitation of national regulation, phase in the nutrition fortifier Nearly double to ordinary nutritional hardening agent chromium content, intake of the human body to trace element chromium can be met
Embodiment described above is a kind of preferable scheme of the present invention, not makees limit in any form to the present invention System also has other variants and remodeling under the premise of without departing from the technical solution recorded in claim.

Claims (9)

1. a kind of preparation method of chromium-rich nutrition fortifier, it is characterised in that：The method step is as follows：
S1：Cleaning：The natural material that will be enriched in Organic Chromium is cleaned 2-3 times with 30-40 DEG C of water and is dried;
S2：It crushes：Clean raw material with pulverizer is crushed, is sieved with 100 mesh sieve;
S3：Superfine grinding：Superfine grinding is carried out to the raw material after crushing using ball milling instrument, milling time 10-30min obtains enzyme Raw material is solved, is put at 0-3 DEG C and carries out preservation, appropriate protection liquid is added in superfine grinding；
S4：Enzymolysis：By the enzymolysis raw material after superfine grinding and water quality than 1:5 mixing, and stir evenly, pH value is adjusted to 5.6- 6.7 or so, it adds in the complex enzyme of material quality 0.8%-1.2% and is stirred continuously, enzymolysis time 2-4h, 45-55 DEG C of hydrolysis temperature；
S5：Enzyme-deactivating：After enzymolysis, enzymolysis liquid is cooled to 30-50 DEG C in 110-120 DEG C, heating 3-5s enzyme deactivations；
S6：It centrifuges：Enzymolysis liquid after inactivation is centrifuged in centrifuge, centrifuge speed 4000- 6000rmp, centrifugation time 5-10min obtain the enzymolysis clear liquid rich in Organic Chromium；
S7：Nanofiltration：The enzymolysis clear liquid that will be enriched in Organic Chromium carries out dialysis concentration with nanometer film, and Organic Chromium is rich in after being filtered Enzymolysis clear liquid；
S8：It is concentrated and dried：By the enzymolysis clear liquid rich in Organic Chromium after filtering in 60-80 DEG C of temperature, vacuum degree -0.075- Solid content is further concentrated under 0.095mpa as 30-40%, moisture content is then dried to obtain in spray dryer again is The powdered Organic Chromium extract of 4%-7%；
S9：Compounding：By Organic Chromium extract and antioxidant, vitamin, protein powder, minerals in mass ratio（1.2-1.5）: （0.8-1.2）:（0.2-0.5）:（0.2-0.5）:（0.6-1.2）It is uniformly mixed, is sieved, is compounded with 100-120 mesh sieve Chromium-rich nutrition fortifier afterwards.
2. the preparation method of chromium-rich nutrition fortifier as described in claim 1, it is characterised in that：The day rich in Organic Chromium Right raw material is enriched-Cr malt, buckwheat or cassia seed.
3. the preparation method of chromium-rich nutrition fortifier as described in claim 1, it is characterised in that：The protection liquid is by following heavy The Raw material processing of amount part forms：1-3 parts of oxidized form of glutathione, 3-6 parts of xanthans, 5-10 parts of avenabeta glucosan, bee glue powder 1-3 parts, 0.5-1 parts of polylysine, 60-80 parts of deionized waters.
4. the preparation method of chromium-rich nutrition fortifier as described in claim 1, it is characterised in that：The complex enzyme is by albumen Enzyme, cellulase and amylase are by weight（0.8-1.2）:（0.8-1.2）:（0.5-1.2）Composition.
5. the preparation method of chromium-rich nutrition fortifier as described in claim 1, it is characterised in that：The antioxidant is day So one or more combination of VE, modified glucan, xylitol or maltitol.
6. the preparation method of chromium-rich nutrition fortifier as described in claim 1, it is characterised in that：The vitamin for VB1, VB2, VB11 or ascorbic one or more combination.
7. the preparation method of chromium-rich nutrition fortifier as described in claim 1, it is characterised in that：The protein powder is： The one or more combination of casein, lactalbumin or pea protein.
8. the preparation method of chromium-rich nutrition fortifier as described in claim 1, it is characterised in that：The minerals are chlorination The one or more combination of calcium, potassium chloride or sodium chloride.
9. the preparation method of chromium-rich nutrition fortifier as claimed in claim 3, it is characterised in that：
The preparation method step of the oxidized form of glutathione is as follows：
1）Reduced glutathione is taken, in 40-45 DEG C of dissolving, the reduced glutathione solution of 50-100g/L is obtained, surveys its pH It is worth for A1;
2) with barium hydroxide tune pH to A2,7.0≤A2≤7.2；
3）A certain amount of hydrogen peroxide is added in, it is stirring while adding, continue to be stirred to react 0.2-0.5h after adding；
4）After reaction, a certain amount of manganese dioxide is added in, stirs 5-10min, then fully filters off manganese dioxide；
5）With sulphur acid for adjusting pH to A3, A1-0.1≤A3≤A1；
6）Barium sulfate precipitate is fully filtered off, spray drying obtains oxidized form of glutathione.
CN201711415130.1A 2017-12-25 2017-12-25 Chromium-rich nutrition fortifier and preparation method thereof Pending CN108201136A (en)

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Application publication date: 20180626