Goal of the invention
The objective of the invention is: the formulation processing method of new type bactericide tisocromide and the agent of N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole list is provided and is main body and other bactericide compounded formulation processing methods with tisocromide and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole, and above-mentioned preparation is applied to field crops, makes it bring into play separately bactericidal effect and delays pesticide resistance.
Summary of the invention
Technical problem to be solved by this invention is: provide tisocromide and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole list agent formulation and with the complex preparation of conventional sterilization agent, estimate the control efficiency of single agent and complex preparation.The present invention solves this technical problem the technical scheme that is adopted: utilize tisocromide and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole and conventional auxiliary agent process single agent and with the complex preparation of conventional sterilization agent, utilize normalized biological activity determination and evaluation method to determine the drug effect of the fungicidal spectrum and the complex preparation of medicament.
A. the fungicidal spectrum of tisocromide and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole:
The plate experiment is found, compound tisocromide of the present invention and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole have excellent bactericidal activity, 2 medicaments are to the Achyla of Oomycete, Aphanomyces, pythium, Phytophthora, Sclerospora, Plasmopara, false Peronospora, a disease that genus produces surplus the Peronospora etc. 20, as seedling blight of rice, the tomato root rot, potato late blight, black shank, the millet powdery mildew, downy mildew of garpe, downy mildew of lettuce, the preventive effect of the disease of plurality of cereals crops such as cucumber downy mildew and economic crops is fine, wherein the fungicidal spectrum of tisocromide and biologically active and metalaxyl are suitable, and the fungicidal spectrum and the biologically active of N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole are higher than metalaxyl.
B. tisocromide and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole are sprouted the allergenic ascus and are had the good restraining effect:
The spore germination experiment finds that N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole suppresses degree to the bacterium of downy mildew of cucumber sporangium and obviously is better than metalaxyl 100 μ g/mL when 5 μ g/mL, and 1 μ g/mL N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole is suitable with 100 μ g/mL metalaxyl inhibiting rates.It is suitable with metalaxyl that tisocromide suppresses degree to the bacterium of downy mildew of cucumber sporangium.
C. the control efficiency of tisocromide and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole:
The experiment of the little strain method of live body finds, N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole obviously is better than metalaxyl to the control efficiency of cucumber downy mildew under 100 μ g/mL concentration suitable with dimethomorph 150 μ g/mL control efficiency.Illustrate that the anti-Peronospora disease effect of cracking down on counterfeiting of N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole is quite remarkable.Tisocromide is suitable to the effect and the metalaxyl of cucumber downy mildew.The experiment of sub-district, field finds that N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole reaches 90.4% at 500 μ g/mL to the cucumber downy mildew control efficiency, is better than the preventive effect under the mancozeb 2000 μ g/mL concentration.
D. the processing and the control efficiency of tisocromide and the agent of N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole list:
The preparation of tisocromide and the agent of N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole list has pulvis, wetting powder, missible oil, granule and colloidal suspending agent and microemulsion, and each preparation obviously is better than the metalaxyl and the dimethomorph of same concentrations to the control efficiency of cucumber downy mildew.
E. the processing and the control efficiency of tisocromide and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole complex preparation:
The preparation of tisocromide and the agent of N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole list has pulvis, wetting powder, missible oil, granule and colloidal suspending agent, can mix the bactericide that uses with tisocromide and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole has: white urea cyanogen, tmtd, ziram, mancozeb, aliette, thiophanate-methyl, tpn, the enemy can be loose, carbendazim, procymidone, iprodione, captan, the mould prestige of second, ester bacterium urea, flutolanil, benomyl, cyproconazole, methasulfocarb, fenpropidin, dislike acid amides, triazolone, thiophanate methyl, metalaxyl, Metalaxyl-M, M 9834, hydroxyisoxazole, dimethomorph, flumorph, tridemorph, Flusilazole, alkene azoles alcohol, dislike mould spirit, Difenoconazole, mepanipyrim, Fluoxastrobin, propiconazole, diazosulfide, salicylic acid, other known any any one or two kinds that can be used as in the bactericide such as tiadinil.Each preparation obviously is better than the metalaxyl and the dimethomorph of same concentrations to the control efficiency of cucumber downy mildew.
In addition, can mix the insecticide that uses with tisocromide and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole has: chlopyrifos, other known any any one or two kinds that can be used as in the insecticide such as inferior Nong, Avermectin, cypermethrin, sumicidin, desinsection list, dimehypo, Imidacloprid, thiophene worm quinoline, Methomyl, flufenoxuron, UC 62644, Avermectin, pleocidin.Each preparation has all kept the effect of tisocromide and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole to the preventive effect of insect pest, the preventive effect of insect has all been kept the effect of corresponding insecticide.
F. the method for processing of tisocromide and each preparation of N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole:
Tisocromide and the agent of N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole list and above-mentioned complex preparation process can be used with inert carrier of using always and the surfactant of using always on the agricultural and mix, also can merge and use with bleeding agent, stabilizing agent, solvent, cosolvent and synergist, intercalating agent etc., carrier can be solid (clay, natural or synthetic silicate) or liquid (water, alcohol etc.), surfactant can be emulsifier, dispersant or the wetting agent of ionic or nonionic, or these surfactant mixtures.
Embodiment 1
The mensuration of tisocromide and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole fungicidal spectrum
N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole has excellent bactericidal activity, belong to the disease that produces to surplus the Achyla of Oomycete, Aphanomyces, pythium, Phytophthora, Sclerospora, Plasmopara, false Peronospora, the Peronospora etc. 20, as multiple melon diseases such as seedling blight of rice, tomato root rot, potato late blight, black shank, millet powdery mildew, downy mildew of garpe, downy mildew of lettuce, cucumber downy mildews good preventive and therapeutic effect is arranged, below compares with metalaxyl and to illustrate biomass growth rate inhibitory action and the fungicidal spectrum of N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole plant species disease fungus surplus 20:
N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole is dissolved in the proper amount of acetone, then with containing the dilution of 200 μ g/ml sorpo1-144 emulsifier aqueous solutions, respectively drawing the 1mL soup then injects in the culture dish, add the 9mL medium more respectively, make the pastille flat board of 12.5 μ g/ml after shaking up, doing blank with the flat board that adds the 1mL aqua sterilisa, is contrast with the metalaxyl; Card punch with diameter 4mm cuts the bacterium dish along the mycelia outer rim, moves on the pastille flat board every processing triplicate, culture dish is placed on cultivation in 24 ± 1 ℃ of constant incubators, and 48 hours " Invest, Then Investigate " bacterium dish expansion diameters are averaged, relatively calculate relative bacteriostasis rate with blank, the results are shown in Table 1:
Table 1 N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole is to plant pathogenic fungi biomass growth rate inhibitory action and fungicidal spectrum
These diseases are fungus-caused by a plurality of genus that are under the jurisdiction of Ascomycota, Basidiomycota, imperfect fungus door, and for example Botrytis, interlink spore genus, black star spore belong to, the spherical shell spore belongs to or the like, have represented the panoramic plant pathogenic fungi of natural world.Table 1 pair 20 plant species disease fungus biomass growth rate inhibitory action find that the mycothallus growth bacteriostasis rate that N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole tries confession is apparently higher than the metalaxyl of 25 μ g/mL under 12.5 μ g/mL concentration.
Embodiment 1 does not limit the present invention, profit use the same method can clear and definite tisocromide fungicidal spectrum and metalaxyl suitable.
Embodiment 2
Tisocromide and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole are to the germination inhibitor of bacterium of downy mildew of cucumber sporangium
Tisocromide and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole 0.5% acetone solution, fresh Pseudoperonospora cubensis is mixed with sporangium liquid, mixes with medicament, establishes 5,1 μ g/mL, metalaxyl (97.3% former powder) is established 100 μ g/mL, the clear water blank, every processing repeats for 4 times, 19 ± 1 ℃ of dark cultivations, number was sprouted and do not sprouted to " Invest, Then Investigate " record sporangium in 6 hours, inhibiting rate is calculated in 20 visuals field of every processing investigation, the results are shown in Table 2.As shown in Table 2, N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole suppresses degree to the bacterium of downy mildew of cucumber sporangium and obviously is better than metalaxyl 100 μ g/mL when 5 μ g/mL, 1 μ g/mL N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole is suitable with 100 μ g/mL metalaxyl inhibiting rates, find in the process of the test, the dark cultivation after 15 hours, the bacterium of downy mildew of cucumber sporangium has developed into the mycelium state in the clear water, and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole is not still sprouted (seeing accompanying drawing 1) at 5 μ g/mL concentration hyposporangiums.
Embodiment 2 does not limit the present invention, and use the same method influence and the metalaxyl can clear and definite tisocromide sporangium sprouted are suitable.
The inhibitory action (%) that table 2 N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole is sprouted sporangium
Embodiment 3
Tisocromide and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole control cucumber downy mildew test effect (the little strain method of live body)
N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole 0.5% acetone solution, emulsified water are mixed with 200,100,50 μ g/mL, and contrast medicine metalaxyl (97.3% former powder) is established 500 μ g/mL, dimethomorph 150 μ g/mL, clear water contrast.2 leaves, 1 heart stage cucumber seedling is inoculated fresh Pseudoperonospora cubensis sporangium liquid, and every processing repeats for 3 times, cultivate at 19 ± 1 ℃ of light dark (14/10), and all " Invest, Then Investigate " state of an illness, 9 grades of record onset states calculate disease and refer to and preventive effect, the results are shown in Table 3.The little strain test of live body shows that it is suitable with dimethomorph 150 μ g/mL preventive effects that N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole preventive effect under 100 μ g/mL concentration obviously is better than metalaxyl, illustrates that the anti-Peronospora disease effect of cracking down on counterfeiting of N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole is quite remarkable.
Embodiment 3 does not limit the present invention, profit use the same method can be clear and definite the live test effect and the metalaxyl of tisocromide control cucumber downy mildew suitable.
The little strain method of the live body result of the test of table 3 N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole control cucumber downy mildew
Embodiment 4
Tisocromide and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole control cucumber downy mildew test (sub-district, field)
N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole 20% wetting powder sample is mixed with 500,400,300 μ g/mL, and 70% mancozeb wettable powder is established 2000,1500 μ g/mL, the clear water contrast, and every processing repeats 3 times.Sprayed 1 time every 7 days, 7 days investigation preventive effects the results are shown in Table 4 after the 3rd time.Test is carried out in the booth in Tianjin Xiqing District Lee seven village.
Table 4 N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole control cucumber downy mildew field trial
As shown in Table 4, N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole 500 μ g/mL reach 90.4% to the cucumber downy mildew control efficiency, are better than the preventive effect under the mancozeb 2000 μ g/mL concentration.
Embodiment 4 does not limit the present invention, and profit uses the same method can clear and definite tisocromide 500 μ g/mL suitable to the preventive effect under cucumber downy mildew control efficiency and the mancozeb 2000 μ g/mL concentration.
Embodiment 5
The formulation processing of tisocromide and the agent of N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole list
N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole 20, sodium lignin sulfonate 2-8, diatomite 10-30, dodecyl sodium sulfate 1-5 (percentage by weight), kaolin supplies 100%, abundant mixing in mixer, method with comminution by gas stream is crushed to 95% mistake, 325 mesh sieves with mixture, gets 20% wetting powder.N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole wettable powder agent prescription is formed can adjust to active ingredient as required between 5% to 95%, and other composition is done corresponding adjustment.The preparation of N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole wetting powder formulation is the former medicine of N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole and above-mentioned raw material to be mixed together the back pulverize in cracker, reaches production standard up to the grain fineness of former medicine and all auxiliary agents and gets final product.
It is identical that the independent processing of N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole can be made the use of pulvis, wetting powder, missible oil, granule and methods such as colloidal suspending agent and microemulsion and conventional bactericide wetting powder, do not enumerate one by one at this.
Embodiment 5 does not limit the present invention, uses the same method to process the various formulations of tisocromide list agent.
Embodiment 6
The formulation processing of tisocromide and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole complex preparation
The first thiophene for gloomy 70% wetting powder process is: N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole 20, mancozeb 50, diatomite 25, dodecyl sodium sulfate 5 (percentage by weight), abundant mixing in mixer, method with comminution by gas stream is crushed to 95% mistake, 325 mesh sieves with mixture, gets first thiophene gloomy 70% wetting powder of generation.
N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole and known biocides, insecticide mixture can be made pulvis, wetting powder, missible oil, granule and methods such as colloidal suspending agent and microemulsion and enumerate no longer one by one, and the principle of carrying out complex preparation is active addition or expanding prevention scope.
Can mix the bactericide that uses with tisocromide and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole has: white urea cyanogen, tmtd, ziram, mancozeb, aliette, thiophanate-methyl, tpn, the enemy can be loose, carbendazim, procymidone, iprodione, captan, the mould prestige of second, ester bacterium urea, flutolanil, benomyl, cyproconazole, methasulfocarb, fenpropidin, dislike acid amides, triazolone, thiophanate methyl, metalaxyl, Metalaxyl-M, M 9834, hydroxyisoxazole, dimethomorph, flumorph, tridemorph, Flusilazole, alkene azoles alcohol, dislike mould spirit, Difenoconazole, mepanipyrim, Fluoxastrobin, propiconazole and diazosulfide, salicylic acid, other known any any one or two kinds that can be used as in the bactericide such as tiadinil.The ratio of tisocromide or N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole can be 5%-95% in each binary mixture of preparation.
Can mix the insecticide that uses with tisocromide and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole has: chlopyrifos, other known any any one or two kinds that can be used as in the insecticide such as inferior Nong, Avermectin, cypermethrin, sumicidin, desinsection list, dimehypo, Imidacloprid, thiophene worm quinoline, Methomyl, flufenoxuron, UC 62644 and Avermectin, pleocidin etc.The ratio of tisocromide or N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole can be 5%-95% in each binary of preparation or 3 yuan of mixtures.
Embodiment 6 does not limit the present invention, uses the same method to process the formulation of the various complex preparations of tisocromide.
Embodiment 7
The be mixed field control effectiveness test of wetting powder control cucumber downy mildew of N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole and mancozeb
The 70% N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole mancozeb wettable powder (70% first thiophene MnZn) that is mixed is mixed with 300,200,100 μ g/mL, and 70% cymoxanil mancozeb wettable powder is established 2000,1500 μ g/mL, the clear water contrast, and every processing repeats 3 times.Sprayed 1 time every 7 days, 7 days investigation preventive effects the results are shown in Table 5 after the 3rd time, and test is carried out in Xiqing District Lee Qi Zhuancheng village, Tianjin booth.
Table 5 N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole mixture control cucumber downy mildew field trial
As can be seen from Table 5,70% N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole mancozeb mixture reaches more than 90% the cucumber downy mildew preventive effect under 200 μ g/mL concentration, with preventive effect under the cymoxanil mancozeb 2000 μ g/mL concentration suitable (accompanying drawing 2), field investigation is found, 70% N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole mancozeb mixture suppresses the scab expansion, make affected part withered, newborn blade is infected less, and the scab place washes sporangium dyeing back and finds that most of sporangiums all lose vitality.
Embodiment 7 does not limit the present invention, the use the same method field efficacy of various formulations of the field efficacy of the various formulations that can measure agent of N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole list and various complex preparations and agent of tisocromide list and various complex preparations of profit.