CN101265240B - Method for preventing and curing plant disease by increasing plant immunity and use thereof - Google Patents
Method for preventing and curing plant disease by increasing plant immunity and use thereof Download PDFInfo
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- CN101265240B CN101265240B CN2008100530816A CN200810053081A CN101265240B CN 101265240 B CN101265240 B CN 101265240B CN 2008100530816 A CN2008100530816 A CN 2008100530816A CN 200810053081 A CN200810053081 A CN 200810053081A CN 101265240 B CN101265240 B CN 101265240B
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Abstract
The invention provides a synthetic method of sodium 4-methyl-1,2,3-thiadiazolyl-5-formate (NK-F002), and provides a plant protective method and application of inducing the immune system of the plant by 4-methyl-1,2,3-thiadiazolyl-5-formic acid (NK-F001), sodium 4-methyl-1,2,3-thiadiazolyl-5-formate (NK-F002), 4-methyl-1,2,3-thiadiazolyl-5-ethyl formate (NK-F003) and methenaimde (SZG-7) to generate resistance against pathogen. The invention also provides an application for improving plant immunity by the combination of two or three of 4-methyl-1,2,3-thiadiazolyl-5-formic acid (NK-F001), sodium 4-methyl-1,2,3-thiadiazolyl-5-formate (NK-F002), 4-methyl-1,2,3-thiadiazolyl-5-ethyl formate (NK-F003), methenaimde (SZG-7), benzthiadiazole (activated ester, BTH), tiadinil (TDL), DL-Beta-aminobutyric acid, virazole, ningnanmycin, antofine, Bingduxing No.1, Bingduxing No.2, XY-13 and XY-30 so as to induce a plant to generate disease resistance, processing dosage forms of single preparation and mixture, and compositions and contents of effective components in the dosage forms. The invention discloses a plant inducing range and induced disease resistance range of compounds and compositions thereof, and application of plant protection in the fields of agriculture and horticulture thereof.
Description
Technical field
Technical scheme of the present invention relates to the method that the compound that utilizes chemosynthesis improves via plant immunity, and these compounds are improving the purposes that agricultural plants and gardening plant opposing pathogenetic bacteria and fungal disease infect.
Background technology
The tradition agricultural chemicals obtains insect protected by directly acting on insect and weeds and pathogen, the purpose of control grass and diseases prevention, these medicaments often cause detrimentally affect to beneficial organism, environment protection is caused more or less harm, seek eco-friendly plant protection method is the direction that the agricultural sciences worker makes great efforts always, the successful development and application of plant activator is exactly the important achievement of this respect research, at worldwide widely-used (the Bakulev V A of plant activator BTH that exploitation is the most successful at present, DehaenW.The Chemistry of 1,2,3-thiadiazole, 2004, John Wiley﹠amp; Sons, Inc.), the plant activator is " environment friendly agricultural " truly, is plant protection from now on and Pesticide Science research key areas and direction.The contriver is from fundamental research, in state natural sciences fund (30270883 and 20672062), under the subsidy of " 973 " plans (2003CB114402) and Tianjin natural science fund (07JCYBJC01200) and Tianjin science and technology support plan International Technology collaborative project (07ZCGHHZ01400), synthetic and discovery N-(the 5-methyl isophthalic acid of design, the 3-thiazol-2-yl)-the 4-methyl isophthalic acid, 2,3-thiadiazoles-5-methane amide (hereinafter to be referred as N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole) has good biological activity (Fan Zhijin etc., novel [1,2,3] thiadiazoles derivative and preparation method and use thereof, People's Republic of China's national inventing patent, publication number: CN1810808A; Fan Zhijin etc. have the new compound of activity of inducing secondary metabolism of Hypericum plant, People's Republic of China's national inventing patent, publication number: CN2020667A); Thereafter, the contriver to this compounds carried out the composition optimizes of system and initiative research (Fan Zhijin, etc., thiobizole formanide derivative and preparation method and use thereof, People's Republic of China's national inventing patent, application number: 200810052546.6; Fan Zhijin, etc., the heterogeneous ring compound and the preparation method and use thereof of thiadiazoles, People's Republic of China's national inventing patent, application number: 200810052547.0 contained; Fan Zhijin, etc., thiadiazoles imine derivative and preparation method and use thereof, People's Republic of China's national inventing patent, application number: 200810052553.6).Finished at present the toxicity test of N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole, N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole is to the LD of the acute oral of female, mouse great and mighty or powerful
50All greater than 5000mg/kg; To acute dermal LD female, mouse great and mighty or powerful
50All greater than 2000mg/kg; Acute eye irritation test result to rabbit is slight the stimulation; Acute skin irritation test result to rabbit is non-stimulated; Sensitization of skin test-results to cavy is weak sensitization.Now determined synthesis route (Fan Zhijin; Deng; the synthesis technique of N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole; People's Republic of China's national inventing patent; application number: 200810052548.5), originally study carefully invention and relate to method and the purposes of these compounds in agricultural plants and gardening plant protection that compounds such as utilizing N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole improves via plant immunity.
Summary of the invention
Technical problem to be solved by this invention is: new 4-methyl isophthalic acid is provided, 2, the synthetic method of 3-thiadiazoles-5-sodium formiate, 4-is provided methyl isophthalic acid, 2,3-thiadiazoles-5-formic acid and 4-methyl isophthalic acid, 2,3-thiadiazoles-5-ethyl formate and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole improve the method for via plant immunity, and utilize these compounds to improve via plant immunity in agriculture field and gardening Application for Field.
The present invention solves this technical problem the technical scheme that is adopted: has the compound that improves via plant immunity and comprises the 4-methyl isophthalic acid, and 2,3-thiadiazoles-5-formic acid and 4-methyl isophthalic acid, 2,3-thiadiazoles-5-ethyl formate and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole and 4-methyl isophthalic acid, 2,3-thiadiazoles-5-sodium formiate; Its chemical structure sees Table 1:
The chemical structure of table 1 compound and test code number
Chemical structure
Test code number NK-F001 NK-F002 NK-F003 N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole (SZG-7)
4-methyl isophthalic acid wherein, 2,3-thiadiazoles-5-formic acid (NK-F001), the 4-methyl isophthalic acid, 2,3-thiadiazoles-5-sodium formiate (NK-F002), the 4-methyl isophthalic acid, 2,3-thiadiazoles-5-ethyl formate (NK-F003) and N-(5-methyl-1,3-thiazole-2-yl)-4-methyl-1,2,3-thiadiazole (SZG-7) can be the compounds of synthetic compound or purchase, the synthetic method of NK-F002 is: 60.98 gram (0.35 mole) NK-F003 are joined in 500 milliliters the there-necked flask, with 16.25 gram (0.39 mole) NaOH (96%) room temperature reaction certain hours in the solution of methyl alcohol, treat the raw material NK-F003 back suction filtration that reacts completely, obtain the light brown solid, drying obtains yellowish brown solid NK-F002, this compound can by after the acidifying again with the NaOH reaction purification; The consumption of this compound can or dwindle by corresponding proportion expansion.
NK-F001, NK-F002, the method that NK-F003 and SZG-7 improve via plant immunity is as follows:
The back is sowed is having in the plastic cup of fertile soil (6 centimetres of diameters, high 9 centimetres) through seed soaking (2 hours, 28 degree), vernalization (24 hours, 28 degree) will to go up No. 5 cucumber variety seeds of Hypon poplar, sows about 4, treats when seedling grows to 2 leaves, 1 heart standby for every glass.Reagent agent with containing each 60 milliliters of soups that are made into a series of gradients behind the dissolved in distilled water of 1 ‰ tween 20s, 1 ‰ soap powder, and is immersed in the soup 30 seconds with the 1st true leaf, and the 2nd not dispenser of true leaf agent is contrast with the clear water.After the dispenser 5 days, buy the bacterium cake with the punch tool of 0.4 centimetre of diameter at the botrytis cinerea colony edge, inoculating needle microtrauma cucumber leaves then, and at wound location inoculation botrytis cinerea cake.Whole strain inoculation, bacterium cake of every leaf inoculation, darkroom, inoculation back 100% relative humidity was preserved moisture 48 hours, take out the back in 26 degree, 60% relative humidity, cultivated under the light dark condition in 10 hours/12 hours, 2-3 days " Invest, Then Investigate " onset states, the blade reverse side is measured lesion area, and calculates disease index, determines to induce effect:
The every strain seedling of disease index=∑ scab diameter/parallel processing sum
Disease index * 100 of induced activity=(disease index of the disease index-control group of treatment group)/control group
Same method can be used for agricultural plants such as anti-cucumber anthracnose of these compound inducing cucumbers and bacterium of downy mildew of cucumber and gardening plant opposing by fungi and bacterial Plant diseases.
The invention has the beneficial effects as follows: the present invention utilizes NK-F001, NK-F002, NK-F003 and SZG-7 can improve the immunizing power of cucumber, strengthen the invasion of the cucumber plant opposing phytopathy original, increase these plants to resistivity by pathogenetic bacteria and fungus-caused Plant diseases thereby this compounds can also be used to improve the immunizing power of other agricultural plantss and gardening plant.
The present invention will more specifically describe by specific preparation and biological activity determination embodiment and utilize NK-F001, NK-F002, NK-F003 and SZG-7 can improve method of immunizing power of cash crop such as cucumber and food crop and garden crop and synthetic method and biological activity and the application thereof of NK-F002, but described embodiment only is used to specify the present invention and unrestricted the present invention, and embodiment is as follows:
Embodiment 1
Synthetic and the structure of compound N K-F002 is identified
With 60.98 gram (0.35 mole) 4-methyl isophthalic acids, 2,3-thiadiazoles-5-ethyl formate (NK-F003) joins in 500 milliliters the there-necked flask, with 16.25 gram (0.39 mole) NaOH (96%) room temperature reaction certain hour in the solution of methyl alcohol, treat the raw material NK-F003 back suction filtration that reacts completely, drying obtains yellowish brown solid NK-F002, this compound can be after acidifying again with the NaOH reaction purification, yield 99%, fusing point: 243-245 degree (decomposition); MS (m/z): 166.57;
1H NMR (solvent: D
2O, chemical shift): 2.580 (3H, s, CH
3); This compound
1H NMR and MS data presentation are consistent with its chemical structure.
Embodiment 2
Utilize NK-F001, NK-F002, NK-F003 and SZG-7 improve the method and the purposes of the immunizing power opposing botrytis cinerea pers of cucumber
Botrytis cinerea pers (Botrytis cinerea) illumination cultivation under 26 degree on the PDA substratum was taken out after 7 days, added 10 ml sterile waters, scraped with transfering loop and got conidium, made spore suspension.The spore suspension of making is filtered with sterile gauze, and filtrate is diluted to spore concentration 10
5-10
6Individual/milliliter is standby, adds 1% sucrose during inoculation, is beneficial to the inducing cucumber morbidity.Choosing 1 heart stage of 2 leaves and blade diameter tests greater than 3 centimetres cucumber seedling.Reagent agent with containing each 60 milliliters of soups that are made into a series of gradients behind the dissolved in distilled water of 1 ‰ tween 20s, 1 ‰ soap powder, and is immersed in the soup 30 seconds with the 1st true leaf, and the 2nd not dispenser of true leaf agent is contrast with the clear water.After the dispenser 5 days, buy the bacterium cake with the punch tool of 0.4 centimetre of diameter at the botrytis cinerea colony edge, inoculating needle microtrauma cucumber leaves then, and at wound location inoculation botrytis cinerea cake.Whole strain inoculation, bacterium cake of every leaf inoculation, the 100% relative humidity 48h that preserves moisture in darkroom, inoculation back takes out the back at 26 degree, and 60% relative humidity is cultivated under 10 hours/12 hours light dark condition.2 days-3 days " Invest, Then Investigate " onset states, the blade reverse side is measured lesion area, and calculates disease index, determines to induce effect:
The every strain seedling of disease index=∑ scab diameter/parallel processing sum
Disease index * 100 of induced activity=(disease index of the disease index-control group of treatment group)/control group
BTH, TDL, SZG-7 and NK-F001, NK-F003, the anti-botrytis cinerea pers of NK-F002 inducing cucumber are handled cucumber seedling according to above-mentioned testing program, in 4 days " Invest, Then Investigate " cucumber onset states of inoculation: concentration not simultaneously BTH lure anti-effect slightly different, best results when concentration is 50 mcg/ml is 60.42%; TDL best results during 5 mcg/ml, it induces effect is 83.33%; NK-F001 best results during 5 mcg/ml, it induces effect is 67.92%; Under the different concns SZG-7 induce anti-effect, be preferably 63.99% relatively during 50 mcg/ml; NK-F003 best results during 5 mcg/ml, it induces effect is 89.58%; 0.5 NK-F002 best results during mcg/ml, it induces effect is 84.58% (table 2).
Table 2 different concns compound is to the effect of inducing of botrytis cinerea pers
The medicament BTH that uses among the present invention, TDL, SZG-7 and NK-F001, NK-F002, NK-F003 mixes use or these medicaments and DL-beta-aminobutyric acid with any two or three, virazole, Ningnanmycin, antofine, No. 1, virus star, No. 2, virus star and XY-13, XY-30 etc. are combined into 2 yuan or 3 yuan of mixtures with arbitrary combination all can obtain equally the effect of inducing as embodiment 2 and embodiment 6 and embodiment 7, the binary mixed ratio of these medicaments is in 1%: 99% to 99%: 1% scope, and the ternary mixed ratio of these medicaments comprises that the ratio of any medicament in mixture all can obtain the effect of inducing of the foregoing description 2 and embodiment 6 and embodiment 7 between 1%: 99%.The formulation that these medicaments can be processed comprises wettable powder, sustained release dosage, aqua, pulvis, micro-capsule suspension, can disperse dense dose, seed treatment emulsion, aqueous emulsion, big granula, granule, microemulsion, oil-suspending agent, finish, the seed with coated pesticidal, suspension concentrates, suspended emulsion agent, water-soluble granule, soluble thick agent, water-dispersible granules etc., and each formulation all can obtain the effect of inducing of the foregoing description 2 and embodiment 6 and embodiment 7.
Embodiment 3
Utilize NK-F001, NK-F002, the activity change of phenylalanine ammonia lyase (PAL) in the cucumber body behind NK-F003 and the SZG-7 processing inducing cucumber challenge inoculation botrytis cinerea pers
The cucumber leaves that newly grow and that inoculate botrytis cinerea pers of learning from else's experience after above-claimed cpd is induced is to be measured, add 3 milliliter of 0.2 mol borate buffer solution (pH8.8, the mercaptoethanol that contains 0.005 mol, 0.001 the EDTA of mol) and the polyvinylpyrrolidone of about example weight 1/10 (PVP), homogenate in ice bath.10000 rev/mins, 4 degree centrifugal 20 minutes down, supernatant liquor is made enzyme activity determination.The mensuration system is by the borate buffer solution of 2 milliliter of 0.2 mol pH8.8.1 milliliter of 0.02 mol L-phenylalanine and 0.8 milliliter of enzyme liquid are formed.After measuring OD290,, measure the OD290 value again, contrast and be 0.8 milliliter of 3 milliliters of enzyme-added liquid of borate buffer solution in 37 degree water-baths 1 hour.The mensuration of protein content adopts Cowes light blue G250 method, is standard protein with BSA.After with medicament was handled the immunity system of inducing cucumber, each is handled the 7th day PAL specific activity of enzyme and all is significantly higher than contrast (table 3), and was consistent with the result of biological assay.
Table 3 medicament is induced and is handled the active variation with challenge inoculation back cucumber leaves PAL
Embodiment 4
Utilize NK-F001, NK-F002, the activity change of polyphenoloxidase (PPO) in the cucumber body behind NK-F003 and the SZG-7 processing inducing cucumber challenge inoculation botrytis cinerea pers
Get bright sample blade 0.5 gram and put into mortar, add 5 milliliter of 0.1 mol borate buffer (pH8.8) and 0.1 gram PVPP and a small amount of quartz sand, ice bath homogenate, centrifugal (4 degree, 12000 rev/mins, 20 minutes) are got supernatant liquor and are measured the PPO vigor.During the PPO vitality test, contain 1.8 milliliter of 0.2 mol phosphoric acid buffer (pH 7.0) in the reaction mixture, 1 milliliter of 0.02 mol pyrocatechol, 0.2 milliliter enzyme liquid, 28 degree reactions add 0.5 milliliter of 6 mol HCl termination reaction after 5 minutes, measure the OD value at 398nm place, changing 0.01 with per minute OD value is an enzyme activity unit (U), is converted into specific activity of enzyme by every milligram of albumen of per minute.After with medicament was handled the immunity system of inducing cucumber, each is handled the 7th day PPO specific activity of enzyme and all is significantly higher than contrast (table 4), and was consistent with the result of biological assay.
The active variation of PPO in the cucumber leaves of inoculation back is induced and challenged to table 4 chemicals treatment
Embodiment 5
Utilize NK-F001, NK-F002, NK-F003 and SZG-7 handle the activity change of back superoxide-dismutase (SOD)
Get bright sample blade 0.5 gram and put into mortar, add 5 milliliter of 0.1 mol borate buffer (pH8.8) and 0.1gPVPP and a small amount of quartz sand, ice bath homogenate, centrifugal (4 degree, 12000 rev/mins, 20 minutes) are got supernatant liquor and are measured the SOD vigor.Get tool plug test tube, add 1.5 milliliter of 0.2 mol phosphoric acid buffer (pH 7.0) respectively, 0.3 milliliter of 130 mmole/rise methionine(Met), 0.3 milliliter of 20 micromoles per liter riboflavin, 0.3 milliliter 750 micromoles per liter nitroblue tetrazolium(NBT)s (NBT), 0.3 milliliter of 100 micromoles per liter EDTA-Na
2, 0.25 ml distilled water and 0.05 milliliter of enzyme liquid.Two contrasts replace enzyme liquid with 0.05 milliliter phosphoric acid buffer (pH 7.8), and wherein a control tube is put dark place, and (25 degree) reaction 20 minutes under 40001x sight lamp of other pipe is a blank with irradiation control tube not.After reaction finishes, measure OD
560, be 1 enzyme activity unit to suppress NBT photochemical reduction 50%, be converted into specific activity of enzyme by every milligram of albumen of per minute.After with medicament was handled the immunity system of inducing cucumber, each is handled the 7th day SOD specific activity of enzyme and all is significantly higher than contrast (table 5), and was consistent with the result of biological assay.
The active variation of SOD in the cucumber leaves of inoculation back is induced and challenged to table 5 chemicals treatment
Embodiment 6
Utilize NK-F001, NK-F002, NK-F003 and SZG-7 improve the method and the purposes of the immunizing power opposing bacterium of downy mildew of cucumber of cucumber
Bacterium of downy mildew of cucumber (Pseudoperonospora cubensis) is taken at the live body host and goes up the bacterium of downy mildew of cucumber of cultivating, and dips in the clear water brush with clean brush pen and gets spore, makes spore suspension, and regulates concentration to 10
5-10
6Individual/milliliter is standby.Choosing 1 leaf, 1 heart stage cucumber seedling tests.Reagent agent with containing each 5 milliliters of soups that are made into a series of gradients behind the dissolved in distilled water of 1 ‰ tween 20s, 1 ‰ soap powder, and is put in order strain with the 0.1MPa air compressor and sprayed medicament, is contrast with the clear water.Dispenser is spray inoculation bacterium of downy mildew of cucumber spore suspension after 24 hours, whole strain inoculation, darkroom, inoculation back 100% relative humidity was preserved moisture 3 days, took out the investigation onset state, state of an illness investigation is a unit with the leaf, and active according to criterion calculation disease index and protection: 0 grade: blade does not have scab; 1 grade: lesion area accounts for whole leaf area below 5%; 3 grades: lesion area accounts for whole leaf area 6-10%; 5 grades: lesion area accounts for whole leaf area 11-25%; 7 grades: lesion area accounts for whole leaf area 26-50%; 9 grades: lesion area accounts for whole leaf area more than 50%.
Disease index=∑ (sick grade blade number * represent progression)/(the representative numerical value of the most serious sick level of blade amt * morbidity)
Disease index * 100 of protection activity=(disease index of the disease index-control group of treatment group)/control group
Handle cucumber seedling according to testing program, inoculate 4 days " Invest, Then Investigate " onset states; Each effect of chemical control of different concns sees Table 6, test-results shows: 40 mcg/ml SZG-7 reach 73.33% to the preventive effect of cucumber downy mildew, and frost occurs that urea cyanogen preventive effect has only 40% with concentration, and the metaxanin preventive effect then is 30%, the preventive effect of Azoxystrobin is best, reaches 100% during 10 mcg/ml.
The different medicaments of table 6 under different concns to the provide protection (be 3 days interval) of cucumber downy mildew
The medicament BTH that uses among the present invention, TDL, SZG-7 and NK-F001, NK-F002, NK-F003 mixes use or these medicaments and DL-beta-aminobutyric acid with any two or three, virazole, Ningnanmycin, antofine, No. 1, virus star, No. 2, virus star and XY-13, XY-30 etc. are combined into 2 yuan or 3 yuan of mixtures with arbitrary combination all can obtain equally the effect of inducing as embodiment 2 and embodiment 6 and embodiment 7, the binary mixed ratio of these medicaments is in 1%: 99% to 99%: 1% scope, and the ternary mixed ratio of these medicaments comprises that the ratio of any medicament in mixture all can obtain the effect of inducing of the foregoing description 2 and embodiment 6 and embodiment 7 between 1%: 99%.The formulation that these medicaments can be processed comprises wettable powder, sustained release dosage, aqua, pulvis, micro-capsule suspension, can disperse dense dose, seed treatment emulsion, aqueous emulsion, big granula, granule, microemulsion, oil-suspending agent, finish, the seed with coated pesticidal, suspension concentrates, suspended emulsion agent, water-soluble granule, soluble thick agent, water-dispersible granules etc., and each formulation all can obtain the effect of inducing of the foregoing description 2 and embodiment 6 and embodiment 7.
Embodiment 7
Utilize NK-F001, NK-F002, NK-F003 and SZG-7 improve the method and the purposes of the immunizing power opposing cucumber anthracnose of cucumber
Cucumber anthracnose (Colletotrichum lagenarium) illumination cultivation under 26 degree on the full nutritional medium was taken out after 7 days, added 10 ml sterile waters, scraped with transfering loop and got conidium, made spore suspension.The spore suspension of making is filtered with sterile gauze, and it is standby that filtrate is diluted to spore concentration 105-106/milliliter, adds 1% sucrose during inoculation, is beneficial to the inducing cucumber morbidity.Choosing 2 leaves, 1 heart stage cucumber seedling tests.Reagent agent with containing each 60 milliliters of soups that are made into a series of gradients behind the dissolved in distilled water of 1 ‰ tween 20s, 1 ‰ soap powder, and is immersed in the soup 30 seconds with the 1st true leaf, and the 2nd not dispenser of true leaf agent is contrast with the clear water.After the dispenser 5 days, spray inoculation cucumber anthracnose spore suspension, whole strain inoculation, darkroom, inoculation back 100% relative humidity was preserved moisture 24 hours, took out the back at 26 degree, and 60% relative humidity is cultivated under 10 hours/12 hours light dark condition.1 all " Invest, Then Investigate " onset states, every glass of seedling state of an illness investigation is according to the whole percentage registration of every glass of seedling, and calculating disease index and induced activity:
Every glass of seedling morbidity of disease index=∑ percentage ratio/parallel processing sum
Disease index * 100 of induced activity=(disease index of the disease index-control group of treatment group)/control group
Handle cucumber seedling according to testing program, concentration not simultaneously BTH lure anti-effect also slightly different, best results when concentration is 25 mcg/ml is 97.14%, when 100 mcg/ml the Folium Cucumidis sativi sector-meeting is produced poisoning; 25 mcg/ml BTH have the different effects of inducing in different intervals, lure anti-best results in the time of 10 days, are 97.14%, afterwards, along with prolongation or the shortening of interval lures anti-effect that reduction is arranged slightly, in the time of 7 days, reduce to 83.07%, in the time of 14 days, reduce to 96.00%; Concentration not simultaneously SZG-7 lure anti-effect slightly different, best results during 5 mcg/ml, preventive effect are 74.98%; Under the same concentrations, the activity of BTH, SZG-7, TDL and the anti-cucumber anthracnose of NK-F001 inducing cucumber plant is slightly different; The effect of BTH is best, and preventive effect reaches 91.42%; The TDL of different concns induces effect TDL best results when 5 mcg/ml when be all 10 days interval, its preventive effect is 83.92%; The NK-F001 of different concns when be all 10 days interval induce effect with 100 mcg/ml the time best results, its preventive effect is at 77.13% (table 7).
Table 7 different concns compound is to the effect of inducing of botrytis cinerea pers
The medicament BTH that uses among the present invention, TDL, SZG-7 and NK-F001, NK-F002, NK-F003 mixes use or these medicaments and DL-beta-aminobutyric acid with any two or three, virazole, Ningnanmycin, antofine, No. 1, virus star, No. 2, virus star and XY-13, XY-30 etc. are combined into 2 yuan or 3 yuan of mixtures with arbitrary combination all can obtain equally the effect of inducing as embodiment 2 and embodiment 6 and embodiment 7, the binary mixed ratio of these medicaments is in 1%: 99% to 99%: 1% scope, and the ternary mixed ratio of these medicaments comprises that the ratio of any medicament in mixture all can obtain the effect of inducing of the foregoing description 2 and embodiment 6 and embodiment 7 between 1%: 99%.The formulation that these medicaments can be processed comprises wettable powder, sustained release dosage, aqua, pulvis, micro-capsule suspension, can disperse dense dose, seed treatment emulsion, aqueous emulsion, big granula, granule, microemulsion, oil-suspending agent, finish, the seed with coated pesticidal, suspension concentrates, suspended emulsion agent, water-soluble granule, soluble thick agent, water-dispersible granules etc., and each formulation all can obtain the effect of inducing of the foregoing description 2 and embodiment 6 and embodiment 7.
Utilize similar method can determine the disease that above-mentioned medicament BTH, TDL, SZG-7 and NK-F001, NK-F003, the anti-wheat diseases of NK-F002 inducing wheat and the anti-rice disease of paddy rice and the anti-vegetable disease of vegetables and the anti-plant pathogenetic bacteria of gardening plant and plant pathogenic fungi cause.These crops comprise: cereal crop (comprise paddy, wheat, barley, oat, corn, millet, Chinese sorghum etc.), tuber crops (comprise sweet potato, potato, cassava etc.), legume crop (comprises soybean, broad bean, pea, mung bean, red bean etc.) fibre crops (cotton, crudefiber crop, silkworm and mulberry etc.), oil crops (peanut, rape, sesame, soybean, Sunflower Receptacle etc.), sugar crop (beet, sugarcane etc.), beverage crops (tealeaves, coffee, cocoa etc.), hobby crop (tobacco leaf etc.), medicinal crop (genseng, the bulb of fritillary etc.), tropical crops (rubber, coconut, oil palm, sisal hemp etc.) food crop and fruit such as, flowers, oil plant, sugar material and cotton, fiber crops, tea, tobacco, cash crop and plantation melons such as Chinese medicinal materials, really, tea, silkworm and mulberry, vegetables (containing various wild vegetable etc.), bamboo shoots, flowers and ornamental plant, hops, medicinal material, pepper, garden crops such as seedling and other garden crops; Disease comprises the disease that the fungi of a plurality of genus of Ascomycota, Basidiomycota, imperfect fungi door such as Achyla, Aphanomyces, pythium, phytophthora, Sclerospora, Plasmopara, false Peronospora, Peronospora produce, as the disease of plurality of cereals crop such as seedling blight of rice, tomato root rot, the late blight of potato, black shank, the downy mildew of millet, downy mildew of garpe, downy mildew of lettuce, cucumber and cash crop.These compounds equally can inducing plant Micobial Disease.
Claims (3)
1.4-methyl isophthalic acid, 2,3-thiadiazoles-5-formic acid is used for inducing the application of food crop and cash crop and garden crop plant fungal disease resistance and Micobial Disease at preparation plant activator; Wherein said crop be selected from paddy, wheat, barley, oat, corn, Chinese sorghum, sweet potato, potato, cassava, soybean, broad bean, pea, mung bean, red bean, cotton, silkworm and mulberry, peanut, rape, sesame, Sunflower Receptacle, beet, sugarcane, coffee, cocoa, genseng, the bulb of fritillary, rubber, coconut, oil palm, sisal hemp, tobacco, melon, really, tea, wild vegetable, bamboo shoots, hops, pepper; Described disease is selected from seedling blight of rice, tomato root rot, the late blight of potato, the black embryo disease of tobacco, the downy mildew of millet, downy mildew of garpe, downy mildew of lettuce, botrytis cinerea pers.
2.4-methyl isophthalic acid, 2,3-thiadiazoles-5-sodium formiate is used for inducing the application of food crop and cash crop and garden crop plant fungal disease resistance and Micobial Disease at preparation plant activator; Wherein said crop be selected from paddy, wheat, barley, oat, corn, Chinese sorghum, sweet potato, potato, cassava, soybean, broad bean, pea, mung bean, red bean, cotton, silkworm and mulberry, peanut, rape, sesame, Sunflower Receptacle, beet, sugarcane, coffee, cocoa, genseng, the bulb of fritillary, rubber, coconut, oil palm, sisal hemp, tobacco, melon, really, tea, wild vegetable, bamboo shoots, hops, pepper; Described disease is selected from seedling blight of rice, tomato root rot, the late blight of potato, black shank, the downy mildew of millet, downy mildew of garpe, downy mildew of lettuce, botrytis cinerea pers.
3.4-methyl isophthalic acid, 2,3-thiadiazoles-5-ethyl formate is used for inducing the application of food crop and cash crop and garden crop plant fungal disease resistance and Micobial Disease at preparation plant activator; Wherein said crop be selected from paddy, wheat, barley, oat, corn, Chinese sorghum, sweet potato, potato, cassava, soybean, broad bean, pea, mung bean, red bean, cotton, silkworm and mulberry, peanut, rape, sesame, Sunflower Receptacle, beet, sugarcane, coffee, cocoa, genseng, the bulb of fritillary, rubber, coconut, oil palm, sisal hemp, tobacco, melon, really, tea, wild vegetable, bamboo shoots, hops, pepper; Described disease is selected from seedling blight of rice, tomato root rot, the late blight of potato, black shank, the downy mildew of millet, downy mildew of garpe, downy mildew of lettuce, botrytis cinerea pers.
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| EP2468097A1 (en) * | 2010-12-21 | 2012-06-27 | Bayer CropScience AG | Use of Isothiazolecarboxamides to create latent host defenses in a plant |
| JP2014088322A (en) * | 2011-01-12 | 2014-05-15 | Nippon Nohyaku Co Ltd | Plant growth regulator and use method thereof |
| CN109452292B (en) * | 2014-04-17 | 2021-04-16 | 四川利尔作物科学有限公司 | Bactericidal composition and application thereof |
| CN105230635B (en) * | 2014-05-30 | 2017-08-11 | 四川利尔作物科学有限公司 | Bactericidal composition and its application |
| CN105284843B (en) * | 2014-06-27 | 2017-07-14 | 利尔化学股份有限公司 | Bactericidal composition |
| CN106489956A (en) * | 2015-09-07 | 2017-03-15 | 四川利尔作物科学有限公司 | Composition pesticide and its application |
| CN106635841A (en) * | 2017-01-10 | 2017-05-10 | 广西大学 | Standing form of botrytis cinerea laboratory material and preparation and application method of standing form |
| CN114946863A (en) * | 2021-12-06 | 2022-08-30 | 南京天秾生物技术有限公司 | Application of 3-methylpyrrolidine-2-carboxylic acid as plant immune inducer |
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