CN100554409C - 使间充质干细胞分化成神经细胞的方法 - Google Patents
使间充质干细胞分化成神经细胞的方法 Download PDFInfo
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Abstract
Description
预处理 | 时间(周) | 接种的细胞数 | 只含有肝细胞生长因子 | 只含有表皮生长因子 | 表皮生长因子和肝细胞生长因子 |
未铺满培养 | 4 | 7.5×10<sup>7</sup> | 没有增殖 | 1×10<sup>5</sup> | 2.0×10<sup>5</sup> |
铺满培养 | 2 | 7.5×10<sup>7</sup> | 没有增殖 | 1.2×10<sup>5</sup> | 1.8×10<sup>5</sup> |
时间(周) | NSE | NeuN | GFAP | MAP-2 | 阴性细胞 | |
只含有表皮生长因子 | 4 | 0.9% | 0.8% | 1.2% | · | 89% |
表皮生长因子和肝细胞生长因子 | 2 | 10% | 25% | 4% | · | 70% |
表皮生长因子和肝细胞生长因子 | 4 | 56% | 75% | 24% | · | 20% |
约24小时铺满培养后,表皮生长因子和肝细胞生长因子 | 2 | 62% | 88% | 31% | 11% | 10% |
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Applications Claiming Priority (1)
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PCT/KR2003/002302 WO2005040362A1 (en) | 2003-10-29 | 2003-10-29 | Method for differentiating mesenchymal stem cell into neural cell and pharmaceutical composition containing the neural cell for neurodegenerative disease |
Publications (2)
Publication Number | Publication Date |
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CN1860224A CN1860224A (zh) | 2006-11-08 |
CN100554409C true CN100554409C (zh) | 2009-10-28 |
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CNB2003801106268A Expired - Fee Related CN100554409C (zh) | 2003-10-29 | 2003-10-29 | 使间充质干细胞分化成神经细胞的方法 |
Country Status (10)
Country | Link |
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US (1) | US7635591B2 (zh) |
EP (1) | EP1687413B8 (zh) |
JP (1) | JP4126060B2 (zh) |
CN (1) | CN100554409C (zh) |
AT (1) | ATE388223T1 (zh) |
AU (1) | AU2003273118B2 (zh) |
CA (1) | CA2543118C (zh) |
DE (1) | DE60319599T8 (zh) |
HK (1) | HK1093755A1 (zh) |
WO (1) | WO2005040362A1 (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103562377A (zh) * | 2011-05-23 | 2014-02-05 | 亚洲大学校产学协力团 | 引入了肝细胞生长因子基因和具有碱性螺旋-环-螺旋基序的神经源性转录因子基因的成人干细胞系及其用途 |
Families Citing this family (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US7807458B2 (en) | 2003-01-30 | 2010-10-05 | The United States Of America As Represented By The Secretary Of The Department Of Veterans Affairs | Multilineage-inducible cells and uses thereof |
DE602004027479D1 (de) * | 2003-04-24 | 2010-07-15 | Koninkl Philips Electronics Nv | Eingriffsfreie links-herzkammervolumenbestimmung |
US20090136461A1 (en) * | 2007-11-28 | 2009-05-28 | Moon Suk Kim | Neuronal differentiation method of adult stem cells using small molecules |
WO2009085216A2 (en) | 2007-12-20 | 2009-07-09 | Squicor | Compositions and methods for detecting or elimninating senescent cells to diagnose or treat disease |
MX339624B (es) | 2008-08-20 | 2016-06-02 | Anthrogenesis Corp | Composiciones mejoradas de celulas y metodos para preparar las mismas. |
US8790638B2 (en) * | 2009-02-04 | 2014-07-29 | Stemedica Cell Technologies, Inc. | Compositions of stem cells and stem cell factors and methods for their use and manufacture |
WO2010111522A2 (en) | 2009-03-26 | 2010-09-30 | The Regents Of The University Of California | Mesenchymal stem cells producing inhibitory rna for disease modification |
CN101948803B (zh) * | 2010-09-13 | 2012-09-05 | 中山大学中山眼科中心 | 人表皮源性间充质干细胞样多能细胞及其制备方法 |
EP2709636A2 (en) * | 2011-05-19 | 2014-03-26 | Ariel-University Research and Development Company Ltd. | Use of mesenchymal stem cells for the improvement of affective and cognitive function |
US20140065110A1 (en) | 2012-08-31 | 2014-03-06 | The Regents Of The University Of California | Genetically modified msc and therapeutic methods |
CN103275070A (zh) * | 2013-05-10 | 2013-09-04 | 郑彪 | 调节单核细胞增殖的四环化合物及其应用 |
CN103919806A (zh) * | 2014-04-09 | 2014-07-16 | 大连医科大学 | 骨髓间充质干细胞作为治疗正己烷致周围性神经病的药物的应用 |
CN104004713A (zh) * | 2014-05-14 | 2014-08-27 | 安沂华 | 脐带间充质干细胞诱导培养为神经细胞的方法 |
CN112646774B (zh) * | 2014-09-11 | 2024-05-17 | 台湾粒线体应用技术股份有限公司 | 用线粒体特化细胞治疗神经退行性疾病的医药组合物 |
US20230346847A1 (en) * | 2020-08-24 | 2023-11-02 | The Johns Hopkins University | Juvenile protective factors to arrest and reverse aging in the enteric nervous system |
Family Cites Families (6)
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US6214334B1 (en) * | 1991-10-21 | 2001-04-10 | Trustees Of The University Of Pennsylvania | Compositions and methods for producing and using homogenous neuronal cell transplants to treat neurodegenerative disorders and brain and spinal cord injuries |
WO1996015226A1 (en) | 1994-11-14 | 1996-05-23 | Neurospheres Holdings Ltd. | Regulation of neural stem cell proliferation |
US5736396A (en) * | 1995-01-24 | 1998-04-07 | Case Western Reserve University | Lineage-directed induction of human mesenchymal stem cell differentiation |
US7015037B1 (en) * | 1999-08-05 | 2006-03-21 | Regents Of The University Of Minnesota | Multiponent adult stem cells and methods for isolation |
KR100449141B1 (ko) | 2001-04-19 | 2004-09-21 | (주)라이프코드 | 간엽 간세포를 신경세포로 분화시키는 방법 |
CA2507395A1 (en) * | 2002-12-02 | 2004-06-17 | Anges Mg, Inc. | Method for culturing neural stem cells using hepatocyte growth factor |
-
2003
- 2003-10-29 AU AU2003273118A patent/AU2003273118B2/en not_active Ceased
- 2003-10-29 CA CA002543118A patent/CA2543118C/en not_active Expired - Fee Related
- 2003-10-29 WO PCT/KR2003/002302 patent/WO2005040362A1/en active IP Right Grant
- 2003-10-29 DE DE60319599T patent/DE60319599T8/de active Active
- 2003-10-29 CN CNB2003801106268A patent/CN100554409C/zh not_active Expired - Fee Related
- 2003-10-29 JP JP2005509875A patent/JP4126060B2/ja not_active Expired - Fee Related
- 2003-10-29 AT AT03754305T patent/ATE388223T1/de active
- 2003-10-29 EP EP03754305A patent/EP1687413B8/en not_active Expired - Lifetime
- 2003-10-29 US US10/595,620 patent/US7635591B2/en not_active Expired - Fee Related
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2007
- 2007-01-04 HK HK07100146.6A patent/HK1093755A1/xx not_active IP Right Cessation
Non-Patent Citations (5)
Title |
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Adult bone marrow stromal cells differentiate into neuralcellsin vitro. SANCHEZ-RAMOS J. ET AL.EXP. NEUROL.,Vol.164 No.2. 2000 |
Adult bone marrow stromal cells differentiate into neuralcellsin vitro. SANCHEZ-RAMOS J. ET AL.EXP. NEUROL.,Vol.164 No.2. 2000 * |
Effects of eight growth factors on the differentiation ofcellsderived from human embryonic stem cells. SCHULDINER M.PROC. NATL. ACAD. SCI. USA,Vol.97 No.21. 2000 |
Effects of eight growth factors on the differentiation ofcellsderived from human embryonic stem cells. SCHULDINER M.PROC. NATL. ACAD. SCI. USA,Vol.97 No.21. 2000 * |
Neuroplacement therapy and stem cell biology underdiseaseconditions'. SUGAYA K. ET AL.CELL. MOL. LIFE SCI.,Vol.60 No.9. 2003 |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103562377A (zh) * | 2011-05-23 | 2014-02-05 | 亚洲大学校产学协力团 | 引入了肝细胞生长因子基因和具有碱性螺旋-环-螺旋基序的神经源性转录因子基因的成人干细胞系及其用途 |
CN103562377B (zh) * | 2011-05-23 | 2015-08-12 | 亚洲大学校产学协力团 | 引入了肝细胞生长因子基因和具有碱性螺旋-环-螺旋基序的神经源性转录因子基因的成人干细胞系及其用途 |
Also Published As
Publication number | Publication date |
---|---|
EP1687413A4 (en) | 2006-11-08 |
HK1093755A1 (en) | 2007-03-09 |
EP1687413A1 (en) | 2006-08-09 |
JP4126060B2 (ja) | 2008-07-30 |
US7635591B2 (en) | 2009-12-22 |
CA2543118C (en) | 2009-10-06 |
AU2003273118A1 (en) | 2005-05-11 |
ATE388223T1 (de) | 2008-03-15 |
EP1687413B1 (en) | 2008-03-05 |
DE60319599T2 (de) | 2008-06-26 |
WO2005040362A1 (en) | 2005-05-06 |
DE60319599D1 (de) | 2008-04-17 |
US20070054399A1 (en) | 2007-03-08 |
CN1860224A (zh) | 2006-11-08 |
CA2543118A1 (en) | 2005-05-06 |
DE60319599T8 (de) | 2008-10-16 |
AU2003273118B2 (en) | 2007-05-17 |
EP1687413B8 (en) | 2008-10-01 |
JP2007528196A (ja) | 2007-10-11 |
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