CN100515189C - Hand labor method for regulating and controlling maturation of sex gland of giant salamander - Google Patents
Hand labor method for regulating and controlling maturation of sex gland of giant salamander Download PDFInfo
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- CN100515189C CN100515189C CNB2006101552936A CN200610155293A CN100515189C CN 100515189 C CN100515189 C CN 100515189C CN B2006101552936 A CNB2006101552936 A CN B2006101552936A CN 200610155293 A CN200610155293 A CN 200610155293A CN 100515189 C CN100515189 C CN 100515189C
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
An artificial regulation method for controlling the development and maturation of sex gland of Chinese drum features that the variation rule of water temp and luminance from the December to the October in next year in natural environment is compressed in 5 months, and the gonadotrophic drug and the parturifacient are injected in different phases.
Description
Technical field
What the present invention relates to is Yi Zhong piscinity gland fully-developed artificial regulatory method, is a kind of by means Cu Shi piscinity gland physics and chemistry fully-developed method in the non-mature period specifically.
Background technology
The fish belongs to Chinese distinctive fish species, and not only delicious meat, and air bladder is individual big, and very high medical value is arranged, and is therefore cultured widely, becomes the good breed variety in East Sea district.In breed, parent population all is to arrest from natural waters at present, because natural waters Zhong De fish is to lay eggs autumn, Suo Yi fish artificial breeding all is to carry out autumn in month at 9-10, and its incubation rate is general 70%, incubation rate general 30%.But because the juvenile fish individuality is very little, survives the winter and can't survive in East Sea district, even it is survived the winter indoor seawater is heated up, its overwintering survival rate also just 50%, the bottleneck of Xing Cheng fish culture development.
Summary of the invention
At above-mentioned this deficiency, for avoiding the juvenile fish difficulty of surviving the winter, the present invention proposes Yi Zhong piscinity gland fully-developed artificial regulatory method Yi Shi piscinity gland and reaches maturity in advance, lay eggs and make the vegetative stage of juvenile fish avoid the arctic weather in winter in spring and summer, finally improve its natural overwintering survival rate.
This invention Ti Gong De piscinity gland fully-developed artificial regulatory method comprises and selects parent population, parent population to go into steps such as feed in the pond, sexual gland is regulated and control accelerating, hasten parturition, wherein
1, select parent population:selecting the age is that 24-26 Yue De fish is as parent population.
2, parent population goes into the pond nursing: go into tank interior December.
2.1, initial environment condition: salinity 22-34 ‰, water temperature 13-15 ℃, illuminance 500-2000Lx, light application time 6 hours, oxygen in water be 0.4-0.6/m greater than 5ppm, little inflation, pneumatic head density
2, 8-12 hour every day stimulation by running water, wherein oxygen in water amount, aeration quantity, charge density and stimulation by running water all remain to the hatching of hastening parturition, all the other conditions are controlled in the requsst listed below.
2.2, bait: in fresh food by weight add vitamin C and vitamin E each 0.1-0.2% by the 3-5% of the fish body weight fresh food of throwing something and feeding every day, and the fresh food kind is by squid, clam worm, plum fish by turns.
3, sexual gland regulation and control accelerating
3.1, environment conditioning: with in the natural environment by the end of December to next year water temperature by the end of October change and light application time change procedure boil down to 5 months;
3.2 gonad maturity accelerating:
3.2.1, in earlier stage, with injection salmon hormone accelerating, dosage is 0.5-1.0 microgram/kilogram fish body weight, per 15 days once, it is double to reach 20 ℃ of beginnings from water temperature.
3.2.2, mid-term, with injection salmon hormone and No. 2 (A2) intermixtures of luteotropin releasing hormone d-ala analog accelerating, dosage: the salmon hormone is 0.5-0.8 microgram/kilogram fish body weight, A2 is 5-20 microgram/kilogram fish body weight, per 15 days once, behind the accelerating in early stage for the second time 15 days, double.
3.2.3, the later stage, with injection luteotropin releasing hormone d-ala analog No. 3 (A3) acceleratings, dosage is 20 micrograms/kilogram fish body weight, every 7-10 day once, behind the second metaphase accelerating 15 days, until gonad maturity.
The gonad maturity accelerating material therefor is identical to female milter dosage.
4, hasten parturition: hasten parturition with human chorionic gonadotrophin (HCG) intermixture with injection A3, maleic acid DOM (DOM), raun dosage: A3 is 10 micrograms/kilogram fish body weight, and DOM is 5 micrograms/kilogram fish body weight, and HCG is 500 international units.Or A3 hastens parturition raun dosage: 20 micrograms/kilogram fish body weight.Milter dosage is 1/2 of raun dosage, carries out when fish body abdominal distention and softness, lays eggs after 30 hours.
The gained ovum is fed with usual method subsequently through getting prelarva with the usual method hatching.
This invention Ti Gong De piscinity gland fully-developed artificial regulatory method is in sexual gland regulation and control accelerating process
Water temperature is adjusted to: this water temperature was kept 20 days after water temperature is cooled to 9 ℃ naturally by 13-15 ℃, in 70 days, be promoted to 28 ℃ of constant keeping 15 days more gradually, in 30 days, be cooled to 24 ℃ again and constantly be maintained until the hatching of hastening parturition, the water temperature rising or falling speed is 0.5-1.0 degree every day, and every lifting 3 degree were stablized 3-6 days;
Illumination is adjusted to: when water temperature is cooled to 9 ℃ naturally by 13-15 ℃, the control illuminance is 500Lx, light application time is to keep 20 days in 6 hours, in 70 days, extend to 18 hours gradually subsequently and kept 20 days, and then shorten gradually and be maintained until the hatching of hastening parturition to 8 hours, the light application time speed of increasing and decrease is at 15-30 minute every day, every increase and decrease 2 hours stable 3-5 days.
This invention Ti Gong De piscinity gland fully-developed artificial regulatory method, by the method for regulating environment and making with medicament carry out gonad maturity accelerating and hasten parturition just before giving birth the time to parent population, making originally lays eggs to control in the autumn lays eggs spring and summer, and then make juvenile fish break away from the threat of harsh and unforgiving environments when surviving the winter, improve survival rate.Breed test through actual, reach 80% through this invention Ti Gong De piscinity gland fully-developed artificial regulatory method fish spawning rate, the incubation rate of being laid eggs is 70%, and the fry rearing survival rate is 40%, and the back overwintering survival rate of passing the winter is more than 70%.This invention Ti Gong De piscinity gland fully-developed artificial regulatory method, not only make and aspect laying eggs, do not seen by this person's wage adjustment control Hou De fish to go down, the hatching aspect of being laid eggs keeps better level too, juvenile fish has grown up enough to resist severe winter when especially arriving winter, can survive the winter naturally in East Sea district, this has the great economy meaning to Ren worker Yang Zhi fishing gear.
Embodiment
1, in 12 months, will be 24-26 month fish parent 22 tails at the monthly age, store up in the indoor 30 cubes round cement pit.When storing up in the pond seawater to record salinity 26 ‰, 15 ℃ of of of of of of water temperature be that, illuminance are 1000Lx, light application time is that 6 hours, oxygen in water are 6ppm, to water body implement little inflation, pneumatic head density is set to 0.5/m2, the stimulation by running water on 12 hours same day.After this, seawater salinity is grasped at 24-26 ‰, and dissolved oxygen is grasped more than 5ppm, and the stimulation by running water time was controlled at 10-12 hour/day, and all the other maintenances are stablized constant.Divide every day and throw in fresh food 2 times, a day injected volume is 3% of a fish body weight, and fresh food with squid, clam worm, plum fish etc. by turns.
In January in next year-end by the end of May, imitation will be by the end of December to next year natural environmental condition by the end of October be compressed in this 5 middle of the month, be about to 10 months water temperature and illumination variation process boil down to 5 months, the stimulation by running water of 8-12 hour every day.Wherein water temperature is regulated: kept 20 days after reducing to 9 ℃ naturally by 15 ℃, in 70 days, be promoted to 28 ℃ of constant keeping 15 days more gradually, be cooled to 24 ℃ again and constantly be maintained until the hatching of hastening parturition in 30 days, the water temperature rising or falling speed is 0.5-1.0 degree every day, and every lifting 3 degree were stablized 3-6 days.Wherein illumination is regulated: after water temperature is reduced to 9 ℃ naturally by 15 ℃, illuminance is adjusted to 500Lx with incandescent lamp, light application time control kept 20 days in 6 hours, in 70 days, extend to 18 hours gradually again and kept 20 days, and then shorten gradually and be maintained until the hatching of hastening parturition to 8 hours, the light application time speed of increasing and decrease is at 15-30 minute every day, every increase and decrease 2 hours stable 3-5 days.Strengthen food nutrition simultaneously and strengthen, divide every day and throw in fresh food 2 times, a day injected volume is 5% of a fish body weight, and fresh food with squid, clam worm, plum fish etc. day by day by turns and add vitamin C and E respectively is the 0.1-0.2% of bait weight.In addition, when water temperature reaches 20 ℃, begin the mode administration accelerating of parent population by injection, its administration time and medicament name of an article consumption such as following table, wherein female milter consumption is identical:
Connect after the above-mentioned last accelerating administration 10 days, parent population abdominal distention degree and soft degree have reached just before giving birth state, hasten parturition by injection system administration to parent population this moment, adopt A3, DOM, HCG intermixture to hasten parturition, dosage raun: A3 is 10 micrograms/kilogram fish body weight, and DOM is 5 micrograms/kilogram fish body weight, HCG is 500 international units, milter: A3 is 5 micrograms/kilogram fish body weight, and DOM is 2.5 micrograms/kilogram fish body weight, and HCG is 250 international units.
By adopting the above-mentioned parents culture and the method for hastening parturition, Huo De fish newly hatched larvae is ten thousand tails surplus in the of totally 152, and fish body total length reaches more than the 15cm before surviving the winter, ten thousand tails surplus in the of totally 61, ten thousand tails surplus the back survival 45 of surviving the winter.
2, the same example is used A3 when wherein hastening parturition, raun dosage 20 micrograms/kilogram fish body weight, and milter dosage 10 micrograms/kilogram fish body weight is injected.
Claims (2)
1, Yi Zhong piscinity gland fully-developed artificial regulatory method is characterized in that comprising and selects parent population, parent population to go into pond nursing, sexual gland regulation and control accelerating, the step of hastening parturition, wherein
I. select parent population:selecting the age is that 24-26 Yue De fish is as parent population;
Ii. parent population goes into the pond nursing: go into tank interior December;
Ii.i. initial environment condition: salinity 22-34 ‰, water temperature 13-15 ℃, illuminance 500-2000Lx, light application time 6 hours, oxygen in water are 0.4-0.6/m greater than 5ppm, little inflation, pneumatic head density
2, 8-12 hour every day stimulation by running water, wherein oxygen in water amount, aeration quantity, charge density and stimulation by running water all remain to the hatching of hastening parturition, all the other conditions are controlled in the requsst listed below;
Ii.ii. bait: every day, this added vitamin C and each 0.1-0.2% of vitamin E by weight in fresh food by the 3-5% of the fish body weight fresh food of throwing something and feeding, and the fresh food kind by squid, clam worm, plum fish by turns;
Iii. sexual gland is regulated and control accelerating
Iii.i. environment conditioning: smooth out with the fingers in the natural environment by the end of December next year water temperature by the end of October and change and light application time change procedure boil down to 5 months;
Iii.ii. gonad maturity accelerating:
Iii.ii.i. in early stage, with injection salmon hormone accelerating, dosage is 0.5-1.0 microgram/kilogram fish body weight, and per 15 days once, and it is double to reach 20 ℃ of beginnings from water temperature;
Iii.ii.ii. mid-term, with injection salmon hormone and No. 2 intermixture acceleratings of luteotropin releasing hormone d-ala analog, dosage: the salmon hormone is 0.5-0.8 microgram/kilogram fish body weight, luteotropin releasing hormone d-ala analog is for No. 2 5-20 microgram/kilogram fish body weight, per 15 days once, behind the accelerating in early stage for the second time 15 days, double;
Iii.ii.iii. later stage, with No. 3 acceleratings of injection luteotropin releasing hormone d-ala analog, dosage is 20 micrograms/kilogram fish body weight, every 7-10 day once, behind the second metaphase accelerating 15 days, until gonad maturity;
The gonad maturity accelerating material therefor is identical to female milter dosage;
Iv. hasten parturition: hasten parturition with No. 3, luteotropin releasing hormone d-ala analog of injection, DOM and HCG intermixture, raun dosage: luteotropin releasing hormone d-ala analog is for No. 3 10 micrograms/kilogram fish body weight, DOM is 5 micrograms/kilogram fish body weight, and HCG is 500 international units, and the milter pharmaceutical quantities is 1/2 of a raun; Or luteotropin releasing hormone d-ala analog is hastened parturition raun dosage for No. 3: 20 micrograms/kilogram fish body weight; Hasten parturition and when fish body abdominal distention and softness, carry out.
2,, it is characterized in that in sexual gland regulation and control accelerating process as claim 1 Suo Shu De piscinity gland fully-developed artificial regulatory method
Water temperature is adjusted to: this water temperature was kept 20 days after water temperature is cooled to 9 ℃ naturally by 13-15 ℃, in 70 days, be promoted to 28 ℃ of constant keeping 15 days more gradually, in 30 days, be cooled to 24 ℃ again and constantly be maintained until the hatching of hastening parturition, the water temperature rising or falling speed is 0.5-1.0 degree every day, and every lifting 3 degree were stablized 3-6 days;
Illumination is adjusted to: when water temperature is cooled to 9 ℃ naturally by 13-15 ℃, the control illuminance is 500Lx, light application time is to keep 20 days in 6 hours, in 70 days, extend to 18 hours gradually subsequently and kept 20 days, and then shorten gradually and be maintained until the hatching of hastening parturition to 8 hours, the light application time speed of increasing and decrease is at 15-30 minute every day, every increase and decrease 2 hours stable 3-5 days.
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| CN104106479B (en) * | 2014-04-02 | 2016-05-25 | 浙江省海洋水产研究所 | A kind of nuisanceless brown croaker breeding technique |
| CN105494185B (en) * | 2015-12-04 | 2017-11-24 | 贵州乌江水电开发有限责任公司 | A kind of method of spring fish artificial propagation |
| CN105746398B (en) * | 2016-03-04 | 2018-10-30 | 镇坪县天源生态养殖有限公司 | A kind of fry hatching method of more squama shovel jaw fishes |
| CN106172106A (en) * | 2016-07-08 | 2016-12-07 | 天津现代晨辉科技集团有限公司 | A kind of Carassius auratus artificial incubation method |
| EA202091334A1 (en) | 2017-12-01 | 2020-09-02 | Стофнфискур Хф | AQUACULTURE METHOD FOR PRODUCING SALMON CAVIAR |
| CN111955388B (en) * | 2020-09-21 | 2022-06-07 | 上海市水产研究所（上海市水产技术推广站） | Method for breeding larval and juvenile pseudosciaena crocea in indoor cement pond |
| CN113854207A (en) * | 2021-11-17 | 2021-12-31 | 甘肃土著鱼类养殖开发有限公司 | Artificial propagation method of Gymnocypris przewalskii |
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