CN100475965C - 一种大肠杆菌高效外分泌表达溶葡萄球菌酶的方法 - Google Patents

一种大肠杆菌高效外分泌表达溶葡萄球菌酶的方法 Download PDF

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CN100475965C
CN100475965C CNB2005100280380A CN200510028038A CN100475965C CN 100475965 C CN100475965 C CN 100475965C CN B2005100280380 A CNB2005100280380 A CN B2005100280380A CN 200510028038 A CN200510028038 A CN 200510028038A CN 100475965 C CN100475965 C CN 100475965C
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staphylococcus lysozyme
expression
lysostaphin
staphylococcus
expressed
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CN1900290A (zh
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黄青山
陆海荣
陆婉英
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SHANGHAI GAOKE UNION BIOTECHNOLOGY DEVELOPMENT Co Ltd
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SHANGHAI GAOKE UNION BIOTECHNOLOGY DEVELOPMENT Co Ltd
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Application filed by SHANGHAI GAOKE UNION BIOTECHNOLOGY DEVELOPMENT Co Ltd filed Critical SHANGHAI GAOKE UNION BIOTECHNOLOGY DEVELOPMENT Co Ltd
Priority to KR1020087001751A priority patent/KR101098353B1/ko
Priority to CN2006800268150A priority patent/CN101228271B/zh
Priority to PCT/CN2006/001640 priority patent/WO2007009351A1/zh
Priority to US11/995,735 priority patent/US8241901B2/en
Priority to JP2008521775A priority patent/JP5242388B2/ja
Priority to EP20060761397 priority patent/EP1918369B1/en
Publication of CN1900290A publication Critical patent/CN1900290A/zh
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    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
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CNB2005100280380A 2005-07-22 2005-07-22 一种大肠杆菌高效外分泌表达溶葡萄球菌酶的方法 Expired - Fee Related CN100475965C (zh)

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Application Number Priority Date Filing Date Title
CNB2005100280380A CN100475965C (zh) 2005-07-22 2005-07-22 一种大肠杆菌高效外分泌表达溶葡萄球菌酶的方法
CN2006800268150A CN101228271B (zh) 2005-07-22 2006-07-11 一种大肠杆菌高效外分泌表达溶葡萄球菌酶的方法
PCT/CN2006/001640 WO2007009351A1 (fr) 2005-07-22 2006-07-11 Méthode d'expression à haut niveau de la sécrétion de lysostaphine dans escherichia coli
US11/995,735 US8241901B2 (en) 2005-07-22 2006-07-11 Method of secretory expression of lysostaphin in Escherichia coli at high level
KR1020087001751A KR101098353B1 (ko) 2005-07-22 2006-07-11 대장균에서 높은 수준의 리소스타핀 분비 발현 방법
JP2008521775A JP5242388B2 (ja) 2005-07-22 2006-07-11 高レベルでの、大腸菌におけるリソスタフィンの分泌発現の方法
EP20060761397 EP1918369B1 (en) 2005-07-22 2006-07-11 A method of secretion expression of lysostaphin in escherichia coli at high level

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CNB2005100280380A CN100475965C (zh) 2005-07-22 2005-07-22 一种大肠杆菌高效外分泌表达溶葡萄球菌酶的方法

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CN1900290A CN1900290A (zh) 2007-01-24
CN100475965C true CN100475965C (zh) 2009-04-08

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CN2006800268150A Active CN101228271B (zh) 2005-07-22 2006-07-11 一种大肠杆菌高效外分泌表达溶葡萄球菌酶的方法

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US (1) US8241901B2 (https=)
EP (1) EP1918369B1 (https=)
JP (1) JP5242388B2 (https=)
KR (1) KR101098353B1 (https=)
CN (2) CN100475965C (https=)
WO (1) WO2007009351A1 (https=)

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CN101302504B (zh) * 2007-05-11 2011-12-21 上海高科联合生物技术研发有限公司 一种抗体亲和层析纯化溶葡萄球菌酶的方法
WO2012137187A1 (en) * 2011-04-08 2012-10-11 Anthem Biosciences Pvt Ltd Novel expression and secretion vector systems for heterologous protein production in escherichia coli
WO2014078373A1 (en) * 2012-11-13 2014-05-22 Iogenetics, Llc Antimicrobial compositions
WO2015020765A2 (en) * 2013-08-06 2015-02-12 Trustees Of Dartmouth College Unglycosylated lysostaphin variant protein
CN103509777B (zh) * 2013-09-30 2016-03-30 中国人民解放军军事医学科学院微生物流行病研究所 一种特异定量检测葡萄球菌的方法
WO2015100448A2 (en) 2013-12-28 2015-07-02 Longhorn Vaccines And Diagnostics, Llc Multimodal antimicrobial therapy
EP3116999B1 (en) 2014-03-14 2021-09-15 F. Hoffmann-La Roche AG Methods and compositions for secretion of heterologous polypeptides
KR20170122216A (ko) 2015-03-06 2017-11-03 제넨테크, 인크. 초순수화 dsba 및 dsbc와 이를 제조하고 사용하는 방법
WO2017201036A1 (en) 2016-05-17 2017-11-23 Genentech, Inc. Stromal gene signatures for diagnosis and use in immunotherapy
CN109563124A (zh) 2016-06-17 2019-04-02 豪夫迈·罗氏有限公司 多特异性抗体的纯化
CN107916244A (zh) * 2017-06-20 2018-04-17 江西嘉博生物工程有限公司 一种表达溶葡球菌素基因的重组乳酸乳球菌的构建方法及应用
CN111560048B (zh) * 2020-06-03 2021-08-13 成都正能生物技术有限责任公司 用于提取金黄色葡萄球菌总蛋白的方法及试剂盒
CN112062820B (zh) * 2020-08-24 2023-05-26 黑龙江八一农垦大学 大肠杆菌重组外膜蛋白a包涵体蛋白的复性纯化方法
CN113005134B (zh) * 2021-03-12 2022-06-03 厦门宝太生物科技股份有限公司 一种促进胶质纤维酸性蛋白在大肠杆菌中大量表达的方法
CN114540385B (zh) * 2022-03-21 2023-06-23 齐鲁工业大学 一种利用大肠杆菌产溶葡萄球菌酶的方法
CN115873834B (zh) * 2022-08-26 2024-06-04 山东大学 一种溶葡萄球菌酶变体及其制备方法和应用
CN115975979A (zh) * 2022-10-28 2023-04-18 硅羿科技（上海）有限公司 一种DNaseI的分泌表达方法

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US3278378A (en) 1962-04-19 1966-10-11 Schindler Charles Alvin Staphylococcus-derived antibiotic
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JP3226289B2 (ja) * 1991-02-13 2001-11-05 株式会社ジャパンエナジー 分泌ベクター、該ベクターで形質転換した微生物及び該微生物から産生される産物の製造法
DE4425645A1 (de) 1994-07-20 1996-02-22 Mueller Karl & Co Kg Deletiertes Lysostaphingen von Staphylococcus simulans
US6132954A (en) * 1996-08-20 2000-10-17 Baylor College Of Medicine Methods of screening for agents that delay a cell cycle and compositions comprising era and an analogue of wild-type era
US6083715A (en) * 1997-06-09 2000-07-04 Board Of Regents, The University Of Texas System Methods for producing heterologous disulfide bond-containing polypeptides in bacterial cells
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MXPA02003885A (es) 1999-10-19 2005-06-03 Bharat Biotech International L Expresion de lisostafina madura recombinante.
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Publication number Publication date
EP1918369B1 (en) 2015-05-20
CN1900290A (zh) 2007-01-24
US20090186380A1 (en) 2009-07-23
JP2009502125A (ja) 2009-01-29
CN101228271A (zh) 2008-07-23
KR20080018960A (ko) 2008-02-28
KR101098353B1 (ko) 2011-12-26
US8241901B2 (en) 2012-08-14
WO2007009351A1 (fr) 2007-01-25
JP5242388B2 (ja) 2013-07-24
CN101228271B (zh) 2011-10-26
EP1918369A1 (en) 2008-05-07
EP1918369A4 (en) 2009-03-25

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