CN100466899C - Method for enhancing wheat ammonium nitrogen-resistant ability in root system environment by LED - Google Patents

Method for enhancing wheat ammonium nitrogen-resistant ability in root system environment by LED Download PDF

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CN100466899C
CN100466899C CNB2005100285844A CN200510028584A CN100466899C CN 100466899 C CN100466899 C CN 100466899C CN B2005100285844 A CNB2005100285844 A CN B2005100285844A CN 200510028584 A CN200510028584 A CN 200510028584A CN 100466899 C CN100466899 C CN 100466899C
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wheat
led
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output
root system
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CN1732752A (en
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陈金星
李止正
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Shanghai Institutes for Biological Sciences SIBS of CAS
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Shanghai Institutes for Biological Sciences SIBS of CAS
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Abstract

The invention belongs to the field of plant culture technique and discloses a device and method, which employs the ability of a kind of light-emitting diode enhancing wheat durability against ammoniacal nitrogen in root environment. The device comprises the following components sequentially: drench and dropping head, black cover coat, cultivating basin and breed sprout of wheat, the cover of bottom basin, locating collar and pump water circulating system etc. The invention employs the light-emitting diode as the plant light supply and the parallel connection of two cultivating device, installing the light supply in the bottom basin equipped with diving pump and thus the plant root in the device can only accept the light brought by water circulation, providing new culture solvent A and B to wheat until earing and blooming. The wheat grows aggressively in the culture solvent with high content of ammoniacal nitrogen, the tillering force is good and the ear-forming rate is high, the total dry weight of single plant and the dry weight of tillering of single plant are high and the increment of leaf length is appreciable.

Description

Light emitting diode strengthens the method for the anti-ammonium nitrogen ability of wheat in root system environment
Technical field
The present invention relates to a kind of plant cultivation technology, especially refer to that a kind of light emitting diode strengthens the devices and methods therefor of the anti-ammonium nitrogen ability of wheat in root system environment, being mainly used in light emitting diode illumination root system has the obvious enhancing wheat nitrogen of anti-ammonium ability, and extensive use in soilless culture system will exert an influence the result to light emitting diode.
Background technology
Wheat is typical dry farming plant, and water culture is difficult for, and generally studies the growth question of wheat without ciltivating process.The sixties in last century, one of the applicant once crossed wheat with the nitrate nitrogen that contains 100% as the water culture of nitrogenous source, but did not see with ammonium nitrogen to be the work of nitrogenous source water planting wheat always.
Most plants all like nitrate nitrogen to make nitrogenous source, though the composition of ammonium nitrogen is also arranged in water planting solution sometimes, are no more than 10% of nitrogen pool usually, make the cultivation failure otherwise the plant ammonia poisoning can take place!
We have used LED illumination plant root system in the water planting environment, observing has good influence and has reported Shen binomial patent of invention (application number 03150726.3/200510024101.3) growth, we cultivate wheat with the ammonium nitrogen that accounts for nitrogen pool 50% earlier in this work, behind the growth certain hour, change 100% ammonium nitrogen solution again into, obtain satisfactory result, show that light emitting diode illumination root system has the obvious enhancing wheat nitrogen of anti-ammonium ability, extensive use in soilless culture system will exert an influence the result to light emitting diode.This work does not appear in the newspapers, special proposition application for a patent for invention.
Summary of the invention
In order to overcome above-mentioned weak point, main purpose of the present invention aims to provide a kind of with the source material of LED as plant, selects typical dry farming plant wheat to do experiment material, handles by doing 4 kinds of light sources respectively, observes its influence to wheat growth; Adopt the way of two cover culturing device in parallel, the LED light source is placed the end basin of immersible pump, make the root system of plant of two covers on the culturing device can only accept the luminous energy effect that brings with water circulation, avoid the direct effect of light fully, record wheat growth situation; Prepare novel culture fluid, the first equilibrium liquid that is grouped into one-tenth such as ammonium nitrogen that contain 50%, the back is with 100% ammonium nitrogen nutrient solution cultivation, strengthens the method for the anti-ammonium nitrogen ability of wheat until the ear light emitting diode of blooming of wheat in root system environment.
The technical problem to be solved in the present invention is: in the root system environment indirect light source is set and need solves following technical problem:
(1) needs the environment of soilless culture, preferably the water planting environment;
(2) light source is stable in water, and influences root system by cycle water;
(3) eliminate fuel factor and issuable other physical effect that irradiation produces in the water;
(4) can carry out the comparative trial of different colours (wave band) light;
(5) to prepare novel culture fluid, until wheat heading relevant technologies problem such as bloom.
The technical solution adopted for the present invention to solve the technical problems is: this device comprises pouring water dropper, cultivation tray alms bowl, illumination system, end basin and pump water circulation system and light emitting diode root system of plant illumination apparatus; Constitute the light source experiment of LED shunting means, by a cover circulation waterway and a cover light emitting diode illumination system two cover root system illumination apparatus in parallel, described root system illumination apparatus or be the monomer culturing device or be binary root system illumination apparatus in parallel, it is characterized in that: described monomer culturing device successively by drench water dropper, black cap rock, cultivation tray alms bowl, Di Pengai, location snare and end basin and each parts of pump water circulation system by wheat kind bud are formed, wherein:
The output of drenching water dropper is connected with the input of black cap rock, the output of black cap rock is connected with the input of the cultivation tray alms bowl that wheat kind bud is arranged, there is the output of the cultivation tray alms bowl of wheat kind bud to be connected with the input of another black cap rock, the output of this black cap rock is connected with the input of end basin lid, the output of end basin lid is connected with the input of location snare, and the output of location snare is connected with the input of end basin with the pump water circulation system.
Described light emitting diode strengthens the device of the anti-ammonium nitrogen ability of wheat in root system environment binary root system illumination apparatus in parallel is not had a light-source system device B by a root system illumination apparatus A and another and is formed, wherein:
One root system illumination apparatus A is successively by drenching water dropper, the black cap rock, the cultivation tray alms bowl that wheat kind bud is arranged, end basin lid, each parts of location snare and end basin are formed, its output of drenching water dropper is connected with the input of black cap rock, the output of black cap rock is connected with the input of the cultivation tray alms bowl that wheat kind bud is arranged, there is the output of the cultivation tray alms bowl of wheat kind bud to be connected with the input of another black cap rock, the output of this black cap rock is connected with the input of end basin lid, the output of end basin lid is connected with the input of location snare, and the output of location snare is connected with the input of end basin with pump water circulation system and illumination system;
Another do not have light-source system device B successively by drench water dropper, black cap rock, cultivation tray alms bowl, Di Pengai, location snare and each parts of end basin by wheat kind bud are formed, the output of drenching water dropper is connected with the input of black cap rock, the output of black cap rock is connected with the input of the cultivation tray alms bowl that wheat kind bud is arranged, there is the output of the cultivation tray alms bowl of wheat kind bud to be connected with the input of another black cap rock, the output of this black cap rock is connected with the input of end basin lid, the output of end basin lid is connected with the input of location snare, and the output of location snare is connected with the input of end basin.
2 equirotal pores of cutter on end basin, one another is by return pipe by upper hose, and the root system illumination apparatus A and the light-source system device B of not having are connected in parallel through above-mentioned two water pipes.
Described light emitting diode strengthens the light source experiment of the device of the anti-ammonium nitrogen ability of wheat in root system environment is made up of four groups of LED shunting means and one group of water planting contrast CK1 and one group of device such as land for growing field crops wheat contrast CK2 with the kind sowing same period, wherein:
Four groups of LED shunting means are respectively: the LED-orchid, LED-is green, LED-is purple and LED-is yellowish green, and by same light power control cabinet control.
Described light emitting diode strengthens the device of the anti-ammonium nitrogen ability of wheat in root system environment black cap rock is the pattern of culturing device assembling back end basin exposed part, and its material is overlapping 4 layers black plastic film.
A kind of light emitting diode strengthens the method for the anti-ammonium nitrogen ability of wheat in root system environment, with the source material of LED as wheat, and place soilless culture or water planting environment to influence root system by cycle water, adopt the way of the above-mentioned culturing device of two covers in parallel, the LED light source places the end basin of immersible pump, make the wheat root of two covers on the culturing device can only accept the luminous energy effect that brings with water circulation, avoid the direct effect of light, prepare novel culture fluid A, B, record wheat growth situation, bloom until the wheat heading, the job step of its plantation and cultivation is:
Step 1. is selected cultivating container for use
Select the installation of cultivating container, making light emitting diode tabula rasa and root illumination facilities for use;
The selected kind of step 2.
Selected winter wheat variety, selected after, with 0.1% mercury chloride sterilization 3 minutes, after the water flushing, in water, soaked 1 day, germinate down in 20 ℃;
Step 3. sowing
Select 3 of of the same size kind of buds, with wet rock wool parcel, put into little cultivation alms bowl, insert in the 5 hole cultivation tray alms bowls again, 15 in 3 every basins in every hole are with the product grow wheat to be the water planting contrast CK1 in the greenhouse;
Novel culture fluid of step 4. and LED light are handled
After planting, each basin is filled with novel culture fluid and begin to circulate purling and irradiation immediately, and the circulation purling time is the 5:30-18:30 of every day, and the LED irradiation time is the 6:00-18:00 of every day, and meanwhile overground part is still accepted solar radiation; Under the pH:5.5-6.5 condition, use in preceding 105 days novel culture fluid A to cultivate earlier, this uses novel culture fluid B cultivation after 105 days;
Step 5. experiment finishes
Look the water consumption situation between culture period and add fresh novel culture fluid, do not change novel culture fluid and finish until experiment, under the LED influence of light, wheat is to the patience of unoptimizable root system environment with examination.
Described novel culture fluid A and B are made up of the compound of following percentage by weight respectively:
Novel culture fluid A:
Ammonium sulfate 0.01~0.03%
Magnesium sulfate 0.01~0.02%
Nitrate of lime 0.03~0.04%
Potassium dihydrogen phosphate 0.01~0.02%
Potassium chloride 0.0045~0.0055%
Calcium chloride 0.01~0.02%
Silicon potash fertilizer 0.0025~0.0035% and
Running water 99.86~99.92%;
Novel culture fluid B:
Ammonium sulfate 0.03~0.05%
Magnesium sulfate 0.01~0.02%
Dipotassium hydrogen phosphate 0.02~0.03%
Calcium chloride 0.02~0.03%
Silicon potash fertilizer 0.0025~0.0035% and
Running water 99.87~99.97%.
The present invention does experiment material with the dry farming wheat, does the LED light source of 4 kinds of root system nutrition liquid respectively and handles, and set LED light source has LED-purple, LED-orchid, LED-is green, LED-is yellowish green.
Root system illumination culturing device adopts two cover culturing device in parallel, puts the LED light source respectively in the end basin that immersible pump is arranged, and makes a shared cover water circulation system of two cover culturing device and a cover light-source system; Wheat is planted respectively in the cultivation tray alms bowl on device end basin, makes the root system of wheat can only accept the indirectly-acting of the light that brings with water circulation, avoids the direct effect of light fully, observes the wheat growth situation.
Preparing novel culture fluid A is: nitrate of lime 0.035%, potassium dihydrogen phosphate 0.014%, potassium chloride 0.005%, silicon potash fertilizer 0.003%, ammonium sulfate 0.02%, magnesium sulfate 0.015%, calcium chloride 0.007%, running water 99.91%, add the LED-light source, pH:5.5-6.5, this is 50% ammonium nitrogen culture fluid;
Preparing novel culture fluid B is: ammonium sulfate 0.04%, and calcium chloride 0.023%, dipotassium hydrogen phosphate 0.023%, magnesium sulfate 0.015%, silicon potash fertilizer 0.003%, running water 99.89% adds the LED-light source and forms, pH:5.5-6.5, this is 100% ammonium nitrogen culture fluid; Only add the used up nutrient solution part of wheat in the training period, all more do not renew liquid, bloom until heading and only finish experiment.
Wherein: the preparation and the using method of trace element:
In 1 premium on currency, dissolve boric acid 24 grams respectively, manganese chloride 14.4 grams, zinc sulphate 1.8 grams, copper sulphate 0.8 gram and sodium molybdate 1.3 grams, this is a mother liquor, with each 20 liters of above-mentioned novel culture fluid A, novel culture fluid B, adds 1 milliliter in this mother liquor;
Chelated iron is b diammonium edta-iron.
The invention has the beneficial effects as follows: in this culturing device, the root of wheat can only be accepted the remote-effects of the LED light source that brings by water circulation.The result has produced obvious cultivation effect, wheat is grown vigorous in 50% and 100% high ammonium state nitrogen solution, performance tillering ability height, percentage of earbearing tiller height, the last gross dry weight height that produces of individual plant seedling, individual plant divides evil dry weight height, and the length of blade increase is obvious etc., shows the outstanding effect of LED illumination root system.
Description of drawings
The present invention is further described below in conjunction with drawings and Examples.
Accompanying drawing 1 is a monomer culturing device schematic diagram of the present invention;
Accompanying drawing 2 is the present invention's two cover culturing device parallel system schematic diagrames;
What accompanying drawing 3 was embodiments of the invention LED-purples to wheat growth influences the sample schematic diagram;
What accompanying drawing 4 was embodiments of the invention LED-Lan to wheat growth influences the sample schematic diagram;
What accompanying drawing 5 was embodiments of the invention LED-purple and LED-orchid to the wheat root growth influences the sample schematic diagram;
What accompanying drawing 6 was embodiments of the invention LEDs-green to wheat growth influences the sample schematic diagram;
What accompanying drawing 7 was embodiments of the invention LEDs-yellowish green to wheat growth influences the sample schematic diagram;
Accompanying drawing 8 is that the green and LED-of the luminous two pipe LED-of embodiments of the invention is yellowish green influences the sample schematic diagram to the wheat root growth;
Label declaration in the accompanying drawing:
1-pouring water dropper; 11-upper hose;
2-black cap rock; 12-return pipe;
3-the cultivation tray alms bowl of wheat kind bud arranged;
4-Di Pengai;
5-location snare;
6-end basin;
7-end basin and pump water circulation system;
8-end basin and pump water circulation system and illumination system;
Embodiment
See also shown in the accompanying drawing 1, the inventive system comprises the pouring water dropper, cultivation tray alms bowl and illumination system, end basin and pump water circulation system and light emitting diode root system of plant illumination apparatus, constitute the light source experiment of LED shunting means, by a cover circulation waterway and a cover light emitting diode illumination system two cover root system illumination apparatus in parallel, described root system illumination apparatus or be used for contrast cultivation or be binary root system illumination apparatus in parallel for the monomer culturing device, described monomer culturing device is successively by drenching water dropper 1, black cap rock 2, cultivation tray alms bowl and wheat kind bud 3, end basin lid 4, location snare 5 and end basin and pump water circulation system 7 wait each parts to form, wherein:
The output of drenching water dropper 1 is connected with the input of black cap rock 2, the output of black cap rock 2 is connected with the input of cultivation tray alms bowl with wheat kind bud 3, the output of cultivation tray alms bowl and wheat kind bud 3 is connected with the input of another black cap rock 2, the output of another black cap rock 2 is connected with the input of end basin lid 4, the output of end basin lid 4 is connected with the input of location snare 5, and the output of location snare 5 is connected with the input of end basin with pump water circulation system 7.
See also shown in the accompanying drawing 2, described light emitting diode strengthens the device of the anti-ammonium nitrogen ability of wheat in root system environment binary root system illumination apparatus in parallel is not had a light-source system device B by a root system illumination apparatus A and another and is formed, wherein:
One root system illumination apparatus A is successively by drenching water dropper 1, black cap rock 2, cultivation tray alms bowl and wheat kind bud 3, end basin lid 4, location snare 5 and end basin 6 wait each parts to form, its output of drenching water dropper 1 is connected with the input of black cap rock 2, the output of black cap rock 2 is connected with the input of cultivation tray alms bowl 3, the output of cultivation tray alms bowl 3 is connected with the input of another black cap rock 2, the output of another black cap rock 2 is connected with the input of end basin lid 4, the output of end basin lid 4 is connected with the input of location snare 5, and the input with illumination system 8 is connected with the pump water circulation system with end basin in the output of location snare 5;
Another does not have light-source system device B successively by drenching water dropper 1, black cap rock 2, cultivation tray alms bowl and wheat kind bud 3, end basin lid 4, location snare 5 and end basin 6 wait each parts to form, the output of drenching water dropper 1 is connected with the input of black cap rock 2, the output of black cap rock 2 is connected with the input of cultivation tray alms bowl with wheat kind bud 3, the output of cultivation tray alms bowl and wheat kind bud 3 is connected with the input of another black cap rock 2, the output of another black cap rock 2 is connected with the input of end basin lid 4, the output of end basin lid 4 is connected with the input of location snare 5, and the output of location snare 5 is connected with the input of end basin 6;
2 equirotal pores of cutter on end basin 6, one is passed through upper hose 11, and another is connected in parallel through two water pipes by return pipe 12, two covering devices.
Described light emitting diode strengthens the light source experiment of the device of the anti-ammonium nitrogen ability of wheat in root system environment is made up of four groups of LED shunting means and one group of water planting contrast CK1 and one group of device such as land for growing field crops wheat contrast CK2 with the kind sowing same period, wherein:
Four groups of LED shunting means are respectively: the LED-orchid, LED-is green, LED-is purple and LED-is yellowish green, and by same light power control cabinet control.
Described light emitting diode strengthens the device of the anti-ammonium nitrogen ability of wheat in root system environment black cap rock 2 is the pattern of assembling back end basin 6 exposed parts, and its material is overlapping 4 layers black plastic film.
A kind of light emitting diode strengthens the method for the anti-ammonium nitrogen ability of wheat in root system environment, with the source material of LED as plant, and place soilless culture or water planting environment to influence root system by cycle water, adopt the way of two cover culturing device in parallel, the LED light source places the end basin 6 of immersible pump, make the root system of plant of two covers on the culturing device can only accept the luminous energy effect that brings with water circulation, avoid the direct effect of light, prepare novel culture fluid A, B, record wheat growth situation, bloom until the wheat heading, the job step of its plantation and cultivation is:
Step 1. is selected cultivating container for use
Select the installation of cultivating container, making light emitting diode tabula rasa and root illumination facilities for use;
The selected kind of step 2.
Selected winter wheat variety, selected after, with 0.1% mercury chloride sterilization 3 minutes, after the water flushing, in water, soaked 1 day, germinate down in 20 ℃;
Step 3. sowing
Select 3 of of the same size kind of buds, with wet rock wool parcel, put into little cultivation alms bowl, insert in the 5 hole cultivation tray alms bowls again, 15 in 3 every basins in every hole are with the product grow wheat to be the water planting contrast CK1 in the greenhouse;
Step 4. nutrient solution and LED light are handled
After planting, each basin is filled with nutrient solution and begin to circulate purling and irradiation immediately, and the circulation purling time is the 5:30-18:30 of every day, and the LED irradiation time is the 6:00-18:00 of every day, and meanwhile overground part is still accepted solar radiation; Under the pH:5.5-6.5 condition, from March 15, in 04 on November 27, to 05, the middle nutrient solution A that uses earlier cultivated in promptly preceding about 105 days, 50% ammonium nitrogen, and after 15,05 on March, promptly this uses nutrient solution B to cultivate 100% ammonium nitrogen after 105 days;
Step 5. experiment finishes
Look the water consumption situation between culture period and add fresh solution, finish not change solution until experiment, under the LED influence of light, wheat is to the patience of unoptimizable root system environment with examination.
Described light emitting diode strengthens the method for the anti-ammonium nitrogen ability of wheat in root system environment the novel culture fluid A of preparation, B are made up of the compound of following percentage by weight:
Culture fluid A:
Ammonium sulfate 0.01~0.03%
Magnesium sulfate 0.01~0.02%
Nitrate of lime 0.03~0.04%
Potassium dihydrogen phosphate 0.01~0.02%
Potassium chloride 0.0045~0.0055%
Calcium chloride 0.01~0.02%
Silicon potash fertilizer 0.0025~0.0035%
Running water 99.86~99.92%;
Culture fluid B:
Ammonium sulfate 0.03~0.05%
Magnesium sulfate 0.01~0.02%
Dipotassium hydrogen phosphate 0.02~0.03%
Calcium chloride 0.02~0.03%
Silicon potash fertilizer 0.0025~0.0035%
Running water 99.87~99.97%
Perhaps:
Culture fluid A in 1 liter of running water:
Ammonium sulfate 0.10~0.20 grams per liter
Magnesium sulfate 0.07~0.15 grams per liter
Nitrate of lime 0.17~0.35 grams per liter
Potassium dihydrogen phosphate 0.07~0.14 grams per liter
Potassium chloride 0.02~0.05 grams per liter
Calcium chloride 0.04~0.08 grams per liter
Silicon potash fertilizer 0.01~0.03 grams per liter
Chelated iron 0.65ml, micro-0.05ml,
1 liter in running water
Culture fluid B:
Ammonium sulfate 0.40~0.80 grams per liter
Magnesium sulfate 0.07~0.15 grams per liter
Dipotassium hydrogen phosphate 0.12~0.23 grams per liter
Calcium chloride 0.11~0.22 grams per liter
Silicon potash fertilizer 0.01~0.03 grams per liter
Chelated iron 0.65ml, micro-0.05ml,
1 liter in running water
The present invention selects typical dry farming plant wheat to do experiment material, does 4 kinds of light sources respectively and handles, and LED-purple, LED-orchid is arranged, LED-is green, LED-is yellowish green, observes its influence to wheat growth.
Adopt the way of two cover culturing device in parallel, the LED light source places the end basin of immersible pump, make the root system of plant of two covers on the culturing device can only accept the luminous energy effect that brings with water circulation, avoid the direct effect of light fully, record wheat growth situation.
Prepare novel culture fluid, earlier use culture fluid A, contain the equilibrium liquid that one-tenths such as 50% ammonium nitrogen is grouped into, culture fluid B is used in the back, contains 100% ammonium nitrogen nutrient solution cultivation, ears until wheat and blooms.
The present invention establishes monomer purling culturing device (as shown in Figure 1) up and down, is used for wheat contrast cultivation, has the black cap rock to avoid natural daylight to disturb;
Two cover monomers are purling culturing device (shown in A, B among Fig. 2) up and down, in end basin parallel connection, with cover water circulation system of one in composite device (A) end basin and illumination system, make wheat root on the stream oriented device can only accept the remote-effects of the LED luminous energy that the cycle water in water-supply line 11 and backwater road 12 carries.
With running water preparation novel wheat culture fluid A, B, only add the used up nutrient solution part of wheat in the training period, all more do not renew liquid, bloom until heading and only finish experiment, wherein: the preparation and the using method of trace element:
In 1 premium on currency, dissolve boric acid 24 grams respectively, manganese chloride 14.4 grams, zinc sulphate 1.8 grams, copper sulphate 0.8 gram, sodium molybdate 1.3 grams, this is a mother liquor, with each 20 liters of above-mentioned nutrient solution A, nutrient solution B, adds 1 milliliter in this mother liquor;
Chelated iron is b diammonium edta-iron.
Specific embodiments of the present invention is:
1), monomer purling culturing device (as shown in Figure 1) up and down, device is formed and installation sees preceding patent (application number 200510024101.3) explanation for details.Mainly be, cut out bigger black plastic film according to the shape of cultivation tray alms bowl upper surface, overlapping 4 layers, And manufactures culture hole thereon, drenches water dropper aqueduct aperture, reaches the axis pipe hole, is used further to assembling (Fig. 1).Black cap rock on the end basin should be manufactured according to the pattern of assembling back end basin exposed part, and material also is overlapping 4 layers black plastic film, and two black effective seal rock prevent that natural daylight from disturbing.
2), purling culturing device (shown in A, B among Fig. 2) about two cover monomers in parallel on the end basin, 2 equirotal pores of cutter on end basin, one connects upper hose 11, another connects return pipe 12, two covering devices and supplies together with a cover water circulation system and a light-source system (Fig. 2) through two water pipe parallel connections.Having 4 groups of shunting means in this work, is respectively the LED-orchid, LED-is green, LED-is purple, LED-is yellowish green, and other has a cover not have the contrast (Fig. 1) of light source.4 groups of experiments are by same light power control cabinet control (number of patent application 03150726.3/200510024101.3), and the latter connects timer.The immersible pump of 5 groups of devices is by the timer-operated terminal plate control of another (number of patent application 03150726.3/200510024101.3).
3), experiment has 6 groups:
4 groups of shunting means such as LED-orchids, LED-is green, LED-is purple, LED-is yellowish green;
1 group of water planting contrast CK1;
The land for growing field crops wheat that other gets with the kind sowing same period is contrast CK2.
4), plantation and cultivation
Experimental period: implement on April 17th, 1 05 on November 27th, 04 in non-automatic control daylight glass room.
Kind, winter wheat, the long-term cultivar of suburb of Shanghai.After selected, the mercury chloride with 0.1% (HgCL) sterilization 3 minutes after the water flushing, was soaked 1 day in water, germinateed down in 20 ℃.
3 of of the same size kind of buds are selected in sowing, with wet rock wool parcel, put into little cultivation alms bowl, insert in the 5 hole cultivation tray alms bowls 15 in 3 every basins in every hole again.With the same product grow wheat of peasant land for growing field crops kind then is water planting contrast CK1 in the greenhouse.
Nutrient solution and LED light are handled, and after planting, each basin is filled with battalion immediately and supported liquid And begin to circulate purling and irradiation.The circulation purling time is the 5:30-18:30 of every day, and the LED irradiation time is the 6:00-18:00 of every day, and meanwhile overground part is still accepted solar radiation.From playing on March 15th, 05 day (on November 27th, 04) of sowing, use 50% ammonium nitrogen nutrient solution cultivation, after this cultivate with 100% ammonium nitrogen nutrient solution.Look the water consumption situation between culture period and add novel solutions, finish not change solution until experiment, under the LED influence of light, wheat is to the patience of unoptimizable root system environment with examination.
Experimental result of the present invention and analysis:
Wheat growth to the shooting stage (after planting 3 months approximately) is observed following result;
See also shown in the accompanying drawing 3, accompanying drawing 3 be light emitting diode (LED-purple) to wheat growth influence the sample schematic diagram, its left figure is: contrast (not irradiation); Right figure is: two cover shunting means, and the shared LED-violet source of root system connects by cycle water;
See also shown in the accompanying drawing 4, accompanying drawing 4 be light emitting diode (LED-orchid) to wheat growth influence the sample schematic diagram, its left figure is: contrast (not irradiation); Right figure is: two cover shunting means, and the shared LED-blue streak source of root system connects by cycle water;
See also shown in the accompanying drawing 5, accompanying drawing 5 be luminous two pipes (LED-purple and LED-orchid) to the wheat root growth influence the sample schematic diagram, its left figure is: the blue processing of LED-; Middle figure is: contrast (not irradiation); Right figure is: LED-is purple to be handled;
See also shown in the accompanying drawing 6, accompanying drawing 6 be light emitting diode (LED-is green) to wheat growth influence the sample schematic diagram, its left figure is: contrast (not irradiation); Right figure is: two cover shunting means, and the shared LED-green light source of root system connects by cycle water;
See also shown in the accompanying drawing 7, accompanying drawing 7 be light emitting diode (LED-is yellowish green) to wheat growth influence the sample schematic diagram, its left figure is: contrast (not irradiation); Right figure is: two cover shunting means, and the shared yellowish green light source of LED-of root system connects by cycle water;
See also shown in the accompanying drawing 8, accompanying drawing 8 be luminous two pipes (LED-green and LED-yellowish green) to the wheat root growth influence the sample schematic diagram, its left figure is: the green processing of LED-; Middle figure is: contrast (not irradiation); Right figure is: the yellowish green processing of LED-;
Can find out from The above results, LED-purple (Fig. 3), LED-orchid (Fig. 4) is according to root system, the overground part riotous growth, significantly better than contrast (Fig. 3,4), two root systems of handling are (Fig. 5) also clearly, and the root system of contrast is subjected to the inhibition of 50% ammonium nitrogen, the root amount seldom, and LED-is purple and the root system of the blue processing of LED-is very flourishing.Observe LED-green (Fig. 6) and LED-yellowish green (Fig. 7) two again and handle, both are better than contrast at the tiller of tillering, and the root system total amount of LED-green (Fig. 6) and radical (Fig. 8) are more than contrast, but there is not the tip of a root, so the jaundice of leaf look may be the green injury that causes that calcium deficiency causes of LED-.Different therewith, the yellowish green overground part leaf of LED-look is dark (Fig. 7), and its root system (Fig. 8) also is better than preceding two and handles (Fig. 8), illustrates that the LED-green-yellow light is than the green wheat growth that is more suitable for of simple LED-.But LED-is green and LED-yellowish green two handles in the performance of wheat growth later stage better.
Changed 100% ammonium nitrogen solution on March 15th, 05, examination wheat growth development later stage situation in-17 days on the 4th May (table 1) is as follows:
(continued on next page)
Table 1, wheat growth development later stage situation *
* the data that each LED light is handled in the table are average results of two basin plant quantity, and leaf length and width and tassel data respectively are the mean value of 10 numbers.
Table 1 presentation of results is compared with the land for growing field crops, and the multinomial data of water planting contrast individual plant are all little, and this is the normal result that water planting is not so good as earth culture.Different therewith, 4 kinds of fairly obvious water planting contrast CK1 and land for growing field crops contrast CK2 of being higher than of every data that light is handled show that LED illumination wheat root causes the obvious increase of biological total amount, the anti-obvious increase that brings disaster to out the individual plant assimilation quantity.

Claims (3)

1, a kind of light emitting diode strengthens the device of the anti-ammonium nitrogen ability of wheat in root system environment, and this device comprises pouring water dropper, cultivation tray alms bowl, illumination system, end basin and pump water circulation system and light emitting diode root system of plant illumination apparatus; Constitute the light source experiment of LED shunting means, by a cover circulation waterway and a cover light emitting diode illumination system two cover root system illumination apparatus in parallel, described root system illumination apparatus or be the monomer culturing device or be binary root system illumination apparatus in parallel, it is characterized in that: described monomer culturing device is formed by drenching water dropper (1), black cap rock (2), the cultivation tray alms bowl (3) that wheat kind bud is arranged, Di Pengai (4), location snare (5) and end basin and each parts of pump water circulation system (7) successively, wherein:
The output of drenching water dropper (1) is connected with the input of black cap rock (2), the output of black cap rock (2) is connected with the input of the cultivation tray alms bowl (3) that wheat kind bud is arranged, the output of planting the cultivation tray alms bowl (3) that wheat kind bud is arranged is connected with the input of another black cap rock (2), the output of this black cap rock (2) is connected with the input of end basin lid (4), the output of end basin lid (4) is connected with the input of location snare (5), and the output of location snare (5) is connected with the input of end basin with pump water circulation system (7);
Described light emitting diode strengthens the device of the anti-ammonium nitrogen ability of wheat in root system environment binary root system illumination apparatus in parallel is not had a light-source system device B by a root system illumination apparatus A and another and is formed, wherein:
One root system illumination apparatus A is successively by drenching water dropper (1), black cap rock (2), the cultivation tray alms bowl (3) that wheat kind bud is arranged, end basin lid (4), each parts of location snare (5) and end basin (6) are formed, its output of drenching water dropper (1) is connected with the input of black cap rock (2), the output of black cap rock (2) is connected with the input of the cultivation tray alms bowl (3) that wheat kind bud is arranged, there is the output of the cultivation tray alms bowl (3) of wheat kind bud to be connected with the input of another black cap rock (2), the output of this black cap rock (2) is connected with the input of end basin lid (4), the output of end basin lid (4) is connected with the input of location snare (5), and the input with illumination system (8) is connected with pump water circulation system (7) with end basin in the output of location snare (5);
Another does not have light-source system device B successively by drenching water dropper (1), black cap rock (2), the cultivation tray alms bowl (3) that wheat kind bud is arranged, end basin lid (4), each parts of location snare (5) and end basin (6) are formed, the output of drenching water dropper (1) is connected with the input of black cap rock (2), the output of black cap rock (2) is connected with the input of planting the cultivation tray alms bowl (3) that wheat kind bud is arranged, there is the output of the cultivation tray alms bowl (3) of wheat kind bud to be connected with the input of another black cap rock (2), the output of this black cap rock (2) is connected with the input of end basin lid (4), the output of end basin lid (4) is connected with the input of location snare (5), and the output of location snare (5) is connected with the input of end basin (6);
Go up 2 equirotal pores of cutter at end basin (6), one another is by return pipe (12) by upper hose (11), and the root system illumination apparatus A and the light-source system device B of not having are connected in parallel through above-mentioned two water pipes;
Described light emitting diode strengthens the light source experiment of the device of the anti-ammonium nitrogen ability of wheat in root system environment is made up of four groups of LED shunting means and one group of water planting contrast CK1 and one group of land for growing field crops wheat contrast CK2 with the kind sowing same period, wherein:
Four groups of LED shunting means are respectively: the LED-orchid, LED-is green, LED-is purple and LED-is yellowish green, and by same light power control cabinet control.
2, light emitting diode according to claim 1 strengthens the device of the anti-ammonium nitrogen ability of wheat in root system environment, it is characterized in that: described black cap rock (2) is the pattern of culturing device assembling back end basin (6) exposed part, and its material is overlapping 4 layers black plastic film.
3, a kind of light emitting diode strengthens the method for the anti-ammonium nitrogen ability of wheat in root system environment, it is characterized in that: with the source material of LED as wheat, and place soilless culture or water planting environment to influence root system by cycle water, adopt in parallel two to overlap the way of culturing device according to claim 1, the LED light source places the end basin (6) of immersible pump, make the wheat root of two covers on the culturing device can only accept the luminous energy effect that brings with water circulation, avoid the direct effect of light, prepare novel culture fluid A and B, record wheat growth situation, bloom until the wheat heading, the job step of its plantation and cultivation is:
Step 1. is selected cultivating container for use
Select the installation of cultivating container, making light emitting diode tabula rasa and root illumination facilities for use;
The selected kind of step 2.
Selected winter wheat variety, selected after, with 0.1% mercury chloride sterilization 3 minutes, after the water flushing, in water, soaked 1 day, germinate down in 20 ℃;
Step 3. sowing
Select 3 of of the same size kind of buds, with wet rock wool parcel, put into little cultivation alms bowl, insert in the 5 hole cultivation tray alms bowls again, 15 in 3 every basins in every hole are with the product grow wheat to be the water planting contrast CK1 in the greenhouse;
Novel culture fluid of step 4. and LED light are handled
After planting, each basin is filled with novel culture fluid and begin to circulate purling and irradiation immediately, and the circulation purling time is the 5:30-18:30 of every day, and the LED irradiation time is the 6:00-18:00 of every day, and meanwhile overground part is still accepted solar radiation; Under the pH:5.5-6.5 condition, use in preceding 105 days novel culture fluid A to cultivate earlier, this uses novel culture fluid B cultivation after 105 days;
Step 5. experiment finishes
Look the water consumption situation between culture period and add fresh novel culture fluid, do not change novel culture fluid and finish until experiment, under the LED influence of light, wheat is to the patience of unoptimizable root system environment with examination;
Described novel culture fluid A and B are made up of the compound of following percentage by weight respectively:
Novel culture fluid A:
Ammonium sulfate 0.01~0.03%
Magnesium sulfate 0.01~0.02%
Nitrate of lime 0.03~0.04%
Potassium dihydrogen phosphate 0.01~0.02%
Potassium chloride 0.0045~0.0055%
Calcium chloride 0.01~0.02%
Silicon potash fertilizer 0.0025~0.0035% and
Running water 99.86~99.92%;
Novel culture fluid B:
Ammonium sulfate 0.03~0.05%
Magnesium sulfate 0.01~0.02%
Dipotassium hydrogen phosphate 0.02~0.03%
Calcium chloride 0.02~0.03%
Silicon potash fertilizer 0.0025~0.0035% and
Running water 99.87~99.97%.
CNB2005100285844A 2005-08-08 2005-08-08 Method for enhancing wheat ammonium nitrogen-resistant ability in root system environment by LED Expired - Fee Related CN100466899C (en)

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CN110604017B (en) * 2019-10-22 2021-06-22 山东省农业科学院作物研究所 Promoting wheat NH4+Method and chemical preparation for assimilating and increasing grain yield
CN112956378B (en) * 2021-04-09 2022-06-17 江苏省农业科学院 Application of yellow-green mixed photoplasm in promoting generation of strawberry stolons and promoting method

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Publication number Priority date Publication date Assignee Title
CN1493184A (en) * 2003-09-02 2004-05-05 中国科学院上海生命科学研究院 Light effect of fluorescent diode in soilless cultivation root system environment

Patent Citations (1)

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Publication number Priority date Publication date Assignee Title
CN1493184A (en) * 2003-09-02 2004-05-05 中国科学院上海生命科学研究院 Light effect of fluorescent diode in soilless cultivation root system environment

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Title
发光二极管生物光源及其医学应用研究. 刘江,全文,华南师范大学. 2004 *

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