CN110604017B - Promoting wheat NH4+Method and chemical preparation for assimilating and increasing grain yield - Google Patents

Promoting wheat NH4+Method and chemical preparation for assimilating and increasing grain yield Download PDF

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Publication number
CN110604017B
CN110604017B CN201911005193.9A CN201911005193A CN110604017B CN 110604017 B CN110604017 B CN 110604017B CN 201911005193 A CN201911005193 A CN 201911005193A CN 110604017 B CN110604017 B CN 110604017B
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wheat
preparation
spraying
assimilating
promoting
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CN110604017A (en
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孔令安
冯波
张荣亭
司纪升
王宗帅
张云秀
吕雪梅
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CROP Research Institute of Shandong Academy of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • A01C21/005Following a specific plan, e.g. pattern
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G22/00Cultivation of specific crops or plants not otherwise provided for
    • A01G22/20Cereals
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G7/00Botany in general
    • A01G7/06Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C5/00Fertilisers containing other nitrates
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05CNITROGENOUS FERTILISERS
    • C05C5/00Fertilisers containing other nitrates
    • C05C5/02Fertilisers containing other nitrates containing sodium or potassium nitrate
    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05GMIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00Mixtures of one or more fertilisers with additives not having a specially fertilising activity

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  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Ecology (AREA)
  • Soil Sciences (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Botany (AREA)
  • Forests & Forestry (AREA)
  • Pest Control & Pesticides (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Fertilizers (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention discloses a method for promoting NH of wheat4 +A method and a chemical preparation for assimilating and increasing the yield of grains. The invention is directed to the application of urea or NH due to the basal and/or additional application4 +Excess N fertilizer application, NH appearance in wheat at different periods4 +The phenomenon of overhigh content symptoms is realized by applying different chemical preparations (potassium nitrate, potassium silicate and calcium silicate applied to the soil surface before wheat jointing or applied to furrow, a preparation containing potassium nitrate, potassium silicate and JA mother liquor sprayed in the jointing stage, a preparation B containing potassium nitrate, potassium silicate, calcium silicate and JA mother liquor sprayed in the flowering stage, a preparation B containing potassium nitrate, potassium silicate, calcium silicate and JA mother liquor sprayed in the early stage of filling) at different stages to promote NH of wheat4 +Assimilating, converting into protein, promoting transport of nutritive organs during wheat filling stage, increasing seed yield, and resisting lodging to overcome NH4 +Too high a content causes a series of problems.

Description

Promoting wheat NH4+Method and chemical preparation for assimilating and increasing grain yield
Technical Field
The present invention belongs to the field of regulating and controlling NH of grain crops4 +The technical field of assimilation, in particular to a method for promoting NH of wheat4 +A method and a chemical preparation for converting into protein, promoting the transportation of nutritive organs of wheat in the filling stage and improving the yield of grains.
Background
Wheat is the most important food crop in the world. Wheat is second to rice in China. Nitrogen is one of the essential nutrients for plant growth and development inPlays an extremely important role in the growth and development process of plants, is the main component of protein and nucleotide, and is an important component of chlorophyll, vitamins, alkaloid and certain hormones; the above components are involved in many important metabolic processes in plants. The nitrogen absorbed by plant root system is mainly NH4 +And NO3 -Predominantly, however, most plants will preferentially absorb NH4 +Even if NH4 +Concentration ratio of (A) to (B) of3 -At low concentrations, the plants will also preferentially absorb NH4 +. Therefore, if NH4 +Low assimilation rate, resulting in NH4 +Accumulated in the plant (Mengel and Kirkby, 1987). In addition, at present, the most important and commonly used nitrogen fertilizer in wheat production is urea, and the urea is converted into NH under the action of soil urease4 +NH in the soil and plants due to excessive application of urea and unreasonable application manner4 +And (4) accumulating in a large amount. Excess NH in cells4 +The accumulation causes physiological damage to the plant and often presents NH4 +Symptoms of intoxication (Liao et al, 1994). The specific symptoms include inhibited seed germination, short root system, significantly reduced root-cap ratio, hindered plant overall growth, and death in severe cases (Britto and Krozucker, 2002).
At present, in wheat production, the traditional wrong idea that the more nitrogen fertilizer is, the higher the yield is exists, and the excessive nitrogen application becomes an important factor for limiting the wheat production. Such as urea or NH4 +Excess N fertilizer application, NH appearing in wheat seedling stage4 +The poisoning symptoms comprise that yellow leaves are too many after leaves are turned green (caused by freezing injury in winter), the root system is short and relatively thick, the roots are shallow and shallow in soil, the overground part is vigorous, the leaves and the stems are weak and not straight, and lodging easily occurs in the grouting period, so that serious yield reduction is caused; the leaves in the middle and later stages are dark green and accompanied with green-losing necrotic spots, the filling rate after blooming is low, the wheat is withered and ripe late, the wheat blighted grains are more, the thousand-grain weight is reduced, and the yield is influenced. The kong et al (2014,2017) study found that urea or NH was overdosed4 +-N fertilizerThe metabolic disorder of wheat leaves, the reduction of mechanical strength of the stalks, the increase of lodging rate and the reduction of oxidation resistance. Therefore, in agricultural production, the urea is applied in excess, and even if no obvious NH appears in the growth of wheat4 +The toxic symptoms are also caused by NH4 +Low assimilation, reduced wheat grain yield, and nutrient waste and environmental pollution.
In order to solve the problems, a regulation mechanism and an application method thereof for the wheat filling and the grain yield by suitable exogenous substances are explored. The invention is researching the relief of NH of plants4 +On the basis of toxic mechanism, the method explores and promotes the NH of the wheat4 +The N assimilation and transport fertilization method and the chemical preparation provide a basis for wheat production, cultivation and management, and have important significance for high yield and high efficiency of wheat, ecological safety of China and the like.
Disclosure of Invention
In order to solve the technical problem, the invention provides a method for promoting NH of wheat4 +A method and a chemical preparation for assimilating and increasing the yield of grains. The invention is directed to the application of urea or NH due to the basal and/or additional application4 +Excess N fertilizer application, NH appearance in wheat at different periods4 +The phenomenon of overhigh content symptoms is that chemical preparations for soil application (before wheat jointing) and foliage application (spraying in jointing stage, spraying before blooming and spraying in early filling stage) are respectively prepared, and different chemical preparations are applied in different stages to promote NH of wheat4 +Assimilating, promoting the transportation of nutritive organs of wheat in the grain filling stage, increasing the yield of grains, resisting lodging, and overcoming NH4 +The problems of wheat leaf metabolism disorder, stalk mechanical strength reduction, lodging rate increase, oxidation resistance reduction and the like caused by over-high content.
The invention firstly provides a method for promoting NH of wheat4 +A chemical agent for assimilating and increasing grain yield, comprising:
preparation a (preparation for soil surface broadcasting or furrow application before wheat jointing): 5-8 kg/667m of potassium nitrate26-10 kg/667m potassium silicate25-8 kg/667m calcium silicate2
Preparation B (promoting wheat NH sprayed in jointing stage)4 +Assimilated foliar spray formulations): each 1L consists of the following components: 25-50 g of potassium nitrate, 20-30 g of potassium silicate, 0.1-0.2mL of JA mother liquor and the balance of water;
c preparation (sprayed before blossom to improve wheat NH)4 +Assimilated and depot strength foliar spray formulations): each 1L consists of the following components: 25-50 g of potassium nitrate, 20-30 g of potassium silicate, 5-8 g of calcium silicate, 0.1-0.2mL of JA mother liquor and the balance of water;
d preparation (improving wheat NH sprayed in early filling stage)4 +Leaf spray formulations for assimilation, increased depot strength and transport of nitrogen-containing compounds): each 1L consists of the following components: 25-50 g of potassium nitrate, 0.1-0.2mL of JA mother liquor, 0.1-0.2mL of IAA mother liquor and the balance of water.
The JA mother liquor: dissolving Jasmonic Acid (JA) in dimethyl sulfoxide (DMSO) at a ratio of 1:1(mg: mL), and storing at low temperature;
the IAA mother liquor: auxin (IAA) was dissolved in dimethyl sulfoxide at 0.175:1(mg: mL).
The invention also provides a method for promoting wheat NH4 +Assimilating and increasing the yield of seeds, characterized in that in the growth process of wheat, if NH is found in the wheat plant4 +High content (NH appears in wheat plant)4 +The content is too high, and NH in the root system or the leaves is further detected4 +Content of-N>28 ug/g fresh weight confirmation), the above chemical formulation and the following measures were used to promote the NH of wheat4 +Assimilation improves the seed grain output:
1) if NH is found before the wheat jointing4 +Too high a content (basal and/or dressing urea or NH)4 +Excessive N fertilizer, thick and short root system, yellow leaves due to winter freeze injury, and NH in root system4 +Content of-N>28 ug/g fresh weight)
The preparation A is applied to soil surface in a broadcasting way or in a furrow way, and then is irrigated (according to the irrigation requirement of the soil), so that the preparation A is quickly dissolved into the soil; then spraying a preparation C on the leaf surfaces before blooming; spraying a preparation D on the leaf surfaces at the early stage of grouting;
in addition, the method does not need to apply urea in the soil as in the traditional wheat planting.
2) If the wheat finds plant NH in the jointing stage (after the birth)4 +High content (soft and weak leaves and stems, not straight and straight, NH in leaves)4 +Content of-N>28 ug/g fresh weight)
Spraying the preparation B on the leaf surfaces only once in the jointing stage, and then spraying the preparation C on the leaf surfaces before flowering; spraying a preparation D on the leaf surfaces at the early stage of grouting;
in addition, the method does not need to apply urea in the traditional wheat planting soil.
3) If the plant NH is found before flowering4 +Hyperhydria symptoms (dark green leaves with necrotic spots of green color, NH in leaves4 +Content of-N>28 ug/g fresh weight)
Spraying the preparation C on the leaf surface before blooming; and D, spraying the preparation on the leaf surfaces at the early stage of grouting.
Remarking: 1. before flowering, it is usually within 10 days before wheat flowering. 2. Generally, NH does not reappear in the early stage of grouting4 +The content is too high, and therefore, it is not described. If necessary, spraying the preparation D.
Further, the spraying method for foliage spraying comprises the following steps: spraying after 16:00 points in windless or breezy weather for 1-2 times at intervals of 1-2 days, wherein the spraying dosage is 20L/667m2. The wheat canopy is integrally and uniformly sprayed. If the rain falls within 3 hours after the spraying, the spraying is carried out again the next day.
The technical scheme of the invention has solid biological and chemical foundations and multiple beneficial effects:
(1) NO in potassium nitrate3 -Plays an important role in signal transduction and can regulate and control multiple NH4 +Assimilation of enzyme activity to accelerate NH4 +Transforming and synthesizing protein; applying proper amount of NO in soil3-N, which reduces the absorption of NH by the root system4 +To alleviate its toxicity;
(2) silicon is a heavy constituent of plants, especially plants of gramineous cropsThe essential nutrient elements are the fourth major elements following nitrogen, phosphorus and potassium by the international soil boundary; remove NH by removing4 +Besides toxic action, the mechanical strength of the stem of the crop can be increased, so that the lodging resistance of the wheat in the later growth period can be improved by spraying at the initial jointing stage, the risk caused by natural disasters in the later growth period of the wheat is effectively reduced, and the production safety of the wheat is greatly improved;
(3) k in the above composition+Is a large amount of nutrient elements required by plants and plays a key role in the growth and development of wheat; ca in calcium silicate2+Is an essential element for the elongation of the pollen tube, so that the wheat pollination rate and the small ear grain number can be promoted by spraying before flowering, and the storage strength is improved;
(4) jasmonic acid and IAA have important signal transduction effect in plant, and can regulate plant cell C, N metabolism and promote NH4 +The assimilation efficiency of (1). Therefore, spraying the slurry with other components at the initial stage of grouting increases NH4 +The assimilation efficiency is improved, the jasmonic acid and IAA content of the grains is increased, the storage strength is improved, and the nitrogen-containing compounds are promoted to be transported into the grains.
(5) The preparation of the invention is simple, the method is easy to implement and reliable, and the preparation can be carried out simultaneously with the pest control.
Because of the traditional large-scale nitrogen application concept of farmers or the fact that the nitrogen content of soil is not evaluated, the problem of excessive application is often caused, and NH appears in wheat at different periods4 +Aiming at the problem of overhigh content, the invention establishes a set of systematic remedy measures: chemical preparations for soil application (before wheat jointing) and foliage spraying (spraying in jointing stage, spraying before blooming and spraying in early filling stage) are respectively prepared, and different chemical preparations are applied in different stages to promote NH of wheat4 +Assimilating, promoting the transportation of nutritive organs of wheat in the grain filling stage, increasing the yield of grains, resisting lodging, and overcoming NH4 +The problems of wheat leaf metabolism disorder, stalk mechanical strength reduction, lodging rate increase, oxidation resistance reduction and the like caused by over-high content.
Detailed Description
Example 1
The problems are as follows: high nitrogen plots, with excess urea base (base pure N17.4 kg/667 m)2) More yellow leaves are found in the rising period of wheat, the root system is shallow and is NH4 +-N content 30.22. mu.g/g fresh weight.
The solution measures are as follows:
1. use of soil
Soil furrow application: potassium nitrate 6kg/667m28kg/667m of potassium silicate2Calcium silicate 6kg/667m2(ii) a And (5) immediately irrigating.
2. Foliage spraying
2.2 preparation of the formulation
2.2.1 mother liquor preparation:
JA mother liquor: dissolving Jasmonic Acid (JA) in dimethyl sulfoxide (DMSO) at a ratio of 1:1(mg: mL), and storing at low temperature;
IAA mother liquor: auxin (IAA) was dissolved in dimethyl sulfoxide at 0.175:1(mg: mL) and stored at low temperature.
2.2.2 spraying before flowering
Increase of NH in wheat4 +The leaf surface spraying preparation (1L) with assimilation and strong storehouse consists of the following components: 40g of potassium nitrate, 20g of potassium silicate, 5g of calcium silicate and 0.15mL of JA mother liquor prepared in 2.2.1.
Spraying before blooming: selecting windless or breezy weather, spraying after 16:00 points for 2 times at an interval of 1 day; the preparation is prepared along with spraying, and the spraying amount is as follows: each time 20L/667m2The wheat canopy is integrally and uniformly sprayed.
2.2.3 initial stage of grouting spraying
Increase of NH in wheat4 +The leaf surface spraying preparation (1L) with assimilation and strong storehouse consists of the following components: 50g of potassium nitrate, 0.15mL of JA mother liquor prepared from 2.2.1 and 0.1mL of IAA mother liquor.
Spraying at the initial stage of grouting: selecting windless or breezy weather, spraying after 16:00 o' clock, spraying for 1 time; the preparation is prepared along with spraying, and the spraying amount is as follows: each time 20L/667m2The wheat canopy is integrally and uniformly sprayed.
3. Collecting growth index and yield index
Harvesting in 6 months and 7 days, drying in the sun in a sunning ground for 3 days, and measuring yield. The number of spikes/667 m treated by the method2The grain number of ears, the thousand grain weight and the yield are respectively increased by 3.6 percent, 4.6 percent, 4.5 percent and 13.2 percent compared with the control (the traditional management mode). Shows that the preparation effectively relieves the NH of the wheat after being treated4 +And (4) poisoning.
The traditional management mode is as follows: basic application of N (in the form of urea), P, K compound fertilizer and urea before wheat sowing, wherein the pure N is 17.4kg/667m2The urea is continuously applied in the rising period of the wheat, which is equivalent to 9.2kg/667m of pure N2. No treatment according to the invention was applied.
TABLE 1 Effect of fertilization and formulation spray on wheat grain yield and composition
Figure BDA0002242535910000051
Example 2
The problems are as follows: high-fertilizer land, excessive urea application in early stage of wheat (pure N applied in basal period before sowing wheat is 16.0kg/667 m)2(ii) a Dressing urea in the rising period of wheat, which is equivalent to 11.5kg/667m of pure N2) Before flowering, plant NH appears4 +Symptoms of excess content, NH4 +-N content 31.54. mu.g/g fresh weight.
The solution measures are as follows: spraying the preparation was sprayed as follows.
1. Preparation of mother liquor
JA mother liquor: dissolving jasmonic acid in dimethyl sulfoxide at a ratio of 1:1(mg: mL), and storing at low temperature;
IAA mother liquor: auxin (IAA) was dissolved in dimethyl sulfoxide at 0.175:1(mg: mL).
2. Spraying before blooming
Promoting wheat NH4 +The leaf surface spraying preparation (1L) for assimilating and improving the storehouse strength comprises the following components: 25g of potassium nitrate, 25g of potassium silicate, 6g of calcium silicate and 0.2mL of JA mother liquor prepared in 2.2.1.
Spraying before blooming: the preparation is prepared along with spraying, and the spraying amount is as follows: each time 20L/667m2Selecting calm orSpraying the mixture for 2 times at an interval of 1-2 days in breezy weather after 16:00 points. The canopy is integrally and uniformly sprayed.
3. Spraying at the initial stage of grouting
The foliar spray preparation (1L) consists of the following components: 35g of potassium nitrate, 0.15mL of JA mother liquor prepared in 2.2.1 and 0.2mL of IAA mother liquor.
Spraying at the initial stage of grouting: the preparation is prepared along with spraying, and the spraying amount is as follows: each time 20L/667m2Selecting windless or breezy weather, spraying after 16:00 o' clock for 2 times at an interval of 1-2 days. The canopy is integrally and uniformly sprayed.
4. Collecting growth index and yield index
Harvesting in 6 months and 5 days, drying in the sun for 3 days, and measuring yield and testing seeds. Due to rainfall and strong wind in the middle and later stages of grouting, high-proportion lodging occurs in traditional management, and the application method provided by the invention can be used for effectively promoting and relieving the NH height of the wheat plant4 +The wheat yield is improved by 18.3 percent compared with the traditional management mode of farmers.
The traditional management mode is as follows: basic application of N (in the form of urea), P, K compound fertilizer and urea before wheat sowing, wherein the pure N is 16.0kg/667m2(ii) a Dressing urea in the rising period of wheat, which is equivalent to 11.5kg/667m of pure N2. The foliar spray treatment of the present invention was not employed.
TABLE 2 Effect of spray application of formulations on wheat yield and its composition
Figure BDA0002242535910000061

Claims (8)

1. Promoting wheat NH4 +A chemical agent for assimilating and increasing grain yield, comprising:
preparation A: preparation for soil surface broadcasting or furrow application before wheat jointing: 5-8 kg/667m of potassium nitrate26-10 kg/667m potassium silicate25-8 kg/667m calcium silicate2
Preparation B: promoting wheat NH sprayed in jointing stage4 +Assimilated foliar spray formulations: each 1L consists of the following components: 25-50 g of potassium nitrate, 20-30 g of potassium silicate and 0.1-0.2mL of JA mother liquor;
preparation C: wheat NH improving agent sprayed before flowering4 +Assimilating and strong leaf surface spraying preparation: each 1L consists of the following components: 25-50 g of potassium nitrate, 20-30 g of potassium silicate, 5-8 g of calcium silicate and 0.1-0.2mL of JA mother liquor;
preparation D: improvement of wheat NH sprayed at early stage of grouting4 +Assimilating, improving the storage strength and the foliage spraying preparation transported by nitrogen-containing compounds: each 1L consists of the following components: 25-50 g of potassium nitrate, 0.1-0.2mL of JA mother liquor and 0.1-0.2mL of IAA mother liquor;
the JA mother liquor: a dimethyl sulfoxide solution of jasmonic acid;
the IAA mother liquor: a solution of auxin in dimethylsulfoxide.
2. The promoting NH of wheat of claim 14 +A chemical preparation for assimilating and increasing grain yield, which is characterized in that the JA mother liquor: jasmonic acid was dissolved in dimethyl sulfoxide at 1 mg/mL.
3. The promoting NH of wheat of claim 14 +Assimilating and improve the chemical preparation of seed grain output, characterized by, IAA mother liquor: auxin was dissolved in dimethyl sulfoxide at 0.175 mg/mL.
4. Promoting wheat NH4 +Assimilating and increasing the yield of seeds, characterized in that in the growth process of wheat, if NH is found in the wheat plant4 +Too high a content, using a chemical formulation according to any of claims 1 to 3, and promoting NH in wheat by4 +Assimilation improves the seed grain output:
1) if NH is found before the wheat jointing4 +The content is too high
The preparation A is applied to the soil surface in a broadcasting way or in a furrow way, and then irrigation is carried out, so that the preparation A is quickly dissolved into the soil; then spraying a preparation C on the leaf surfaces before blooming; spraying a preparation D on the leaf surfaces at the early stage of grouting;
2) if plant NH is found in the wheat jointing stage4 +The content is too high
Spraying the preparation B on the leaf surfaces only once in the jointing stage, and then spraying the preparation C on the leaf surfaces before flowering; spraying a preparation D on the leaf surfaces at the early stage of grouting;
3) if the plant NH is found before flowering4 +The content is too high
Spraying the preparation C on the leaf surface before blooming; and D, spraying the preparation on the leaf surfaces at the early stage of grouting.
5. The promoting NH of wheat of claim 44 +A method for assimilating and increasing the yield of grains, characterized in that, the wheat plant NH4 +The content is too high: NH in roots or leaves4 +Content of-N>28. mu.g/g fresh weight.
6. The promoting NH of wheat of claim 44 +Assimilating and increasing the yield of seeds, characterized in that in the growth process of wheat, if NH is found in the wheat plant4 +The content is too high, and urea is not applied in the soil in wheat planting.
7. The promoting NH of wheat of claim 44 +Method for assimilating and increasing the yield of kernels, characterized in that NH is found in the early stages of the filling4 +If the content is too high, the D preparation is adopted for spraying.
8. The promoting NH of wheat of claim 44 +The method for assimilating and increasing the yield of grains is characterized in that the spraying dosage of the preparation B, the preparation C and the preparation D is 20L/667m2The wheat canopy is integrally and uniformly sprayed, and the spraying times of the preparation C and the preparation D are 1-2 times at intervals of 1-2 days.
CN201911005193.9A 2019-10-22 2019-10-22 Promoting wheat NH4+Method and chemical preparation for assimilating and increasing grain yield Expired - Fee Related CN110604017B (en)

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