CL2011000560A1 - Polipeptidos mutantes con actividad delta 5 desaturasa; acido nucleico y celula huesped que los comprenden, y metodo para producir acido araquidonico o acido eicosapentaenoico. - Google Patents

Polipeptidos mutantes con actividad delta 5 desaturasa; acido nucleico y celula huesped que los comprenden, y metodo para producir acido araquidonico o acido eicosapentaenoico.

Info

Publication number
CL2011000560A1
CL2011000560A1 CL2011000560A CL2011000560A CL2011000560A1 CL 2011000560 A1 CL2011000560 A1 CL 2011000560A1 CL 2011000560 A CL2011000560 A CL 2011000560A CL 2011000560 A CL2011000560 A CL 2011000560A CL 2011000560 A1 CL2011000560 A1 CL 2011000560A1
Authority
CL
Chile
Prior art keywords
acid
delta
host cell
nucleic acid
desaturase activity
Prior art date
Application number
CL2011000560A
Other languages
English (en)
Spanish (es)
Inventor
Qun Zhu
Dana M Walters Pollak
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of CL2011000560A1 publication Critical patent/CL2011000560A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
CL2011000560A 2008-09-19 2011-03-17 Polipeptidos mutantes con actividad delta 5 desaturasa; acido nucleico y celula huesped que los comprenden, y metodo para producir acido araquidonico o acido eicosapentaenoico. CL2011000560A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US9833308P 2008-09-19 2008-09-19

Publications (1)

Publication Number Publication Date
CL2011000560A1 true CL2011000560A1 (es) 2011-10-07

Family

ID=42038064

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2011000560A CL2011000560A1 (es) 2008-09-19 2011-03-17 Polipeptidos mutantes con actividad delta 5 desaturasa; acido nucleico y celula huesped que los comprenden, y metodo para producir acido araquidonico o acido eicosapentaenoico.

Country Status (10)

Country Link
US (3) US8268598B2 (enExample)
EP (1) EP2324119B1 (enExample)
JP (1) JP5762291B2 (enExample)
CN (1) CN102197047B (enExample)
AU (1) AU2009293160B2 (enExample)
BR (1) BRPI0913714A2 (enExample)
CA (1) CA2735379A1 (enExample)
CL (1) CL2011000560A1 (enExample)
DK (1) DK2324119T3 (enExample)
WO (1) WO2010033753A2 (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010033753A2 (en) * 2008-09-19 2010-03-25 E. I. Du Pont De Nemours And Company Mutant delta5 desaturases and their use in making polyunsaturated fatty acids
CN103282474A (zh) 2010-04-22 2013-09-04 纳幕尔杜邦公司 从微生物生物质中获得包含多不饱和脂肪酸的组合物的方法
WO2011154947A2 (en) * 2010-06-10 2011-12-15 Ben-Gurion University Of The Negev Research And Development Authority D5 desaturase-defective mutant gene and use thereof
AU2011293167B2 (en) 2010-08-26 2016-12-08 E. I. Du Pont De Nemours And Company Mutant delta-9 elongases and their use in making polyunsaturated fatty acids
CN103249834B (zh) 2010-08-26 2016-10-26 纳幕尔杜邦公司 生产高水平二十碳五烯酸的重组微生物宿主细胞
CN103080317B (zh) * 2010-08-26 2017-07-11 纳幕尔杜邦公司 突变型hpgg基序和hdash基序δ‑5去饱和酶以及它们在制备多不饱和脂肪酸中的用途
CA2824584A1 (en) 2010-12-30 2012-07-05 E. I. Du Pont De Nemours And Company Use of saccharomyces cerevisiae suc2 gene in yarrowia lipolytica for sucrose utilization
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
CN103841825B (zh) 2011-02-11 2017-03-22 纳幕尔杜邦公司 从微生物生物质中获取含脂质组合物的方法
CA2877279A1 (en) * 2012-06-19 2013-12-27 E. I. Du Pont De Nemours And Company Improved production of polyunsaturated fatty acids by coexpression of acyl-coa:lysophosphatidylcholine acyltransferases and phospholipid:diacylglycerol acyltransferases
CN102978177B (zh) * 2012-11-09 2015-06-03 浙江工业大学 冬虫夏草合成代谢二十碳五烯酸的△-5-脱氢酶、基因及应用
WO2014074772A1 (en) 2012-11-09 2014-05-15 Heliae Development, Llc Mixotrophic, phototrophic, and heterotrophic combination methods and systems
WO2014074770A2 (en) 2012-11-09 2014-05-15 Heliae Development, Llc Balanced mixotrophy methods
US9005940B2 (en) 2012-12-21 2015-04-14 E I Du Pont De Nemours And Company Down-regulation of a polynucleotide encoding a Sou2 sorbitol utilization protein to modify lipid production in microbial cells
JP2014193153A (ja) * 2013-02-28 2014-10-09 Euglena Co Ltd ユーグレナへの遺伝子導入方法
JP2014193154A (ja) * 2013-02-28 2014-10-09 Euglena Co Ltd ユーグレナの形質転換体
BR112015020827A2 (pt) * 2013-02-28 2017-10-10 Euglena Co Ltd método para a introdução de gene em euglena, e transformante do mesmo
DK3666082T3 (da) 2018-12-12 2022-06-20 Nippon Suisan Kaisha Ltd En sammensætning indeholdende stærkt umættet fedtsyre eller alkylester deraf og en fremgangsmåde til fremstilling af samme

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5972664A (en) 1997-04-11 1999-10-26 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
PT2166069T (pt) * 2003-08-01 2016-09-13 Basf Plant Science Gmbh Método de produção de ácidos gordos polinsaturados em organismos transgénicos
US7678560B2 (en) * 2006-05-17 2010-03-16 E.I. Du Pont De Nemours And Company Δ 5 desaturase and its use in making polyunsaturated fatty acids
US7695950B2 (en) * 2006-05-17 2010-04-13 E. I. Du Pont De Nemours And Company Δ5 desaturase and its use in making polyunsaturated fatty acids
US8846374B2 (en) 2006-12-12 2014-09-30 E I Du Pont De Nemours And Company Carotenoid production in a recombinant oleaginous yeast
WO2009046231A1 (en) * 2007-10-03 2009-04-09 E. I. Du Pont De Nemours And Company Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production
WO2010033753A2 (en) * 2008-09-19 2010-03-25 E. I. Du Pont De Nemours And Company Mutant delta5 desaturases and their use in making polyunsaturated fatty acids

Also Published As

Publication number Publication date
US8367383B2 (en) 2013-02-05
AU2009293160A1 (en) 2010-03-25
EP2324119A2 (en) 2011-05-25
EP2324119A4 (en) 2013-05-01
EP2324119B1 (en) 2016-03-30
CA2735379A1 (en) 2010-03-25
US8614075B2 (en) 2013-12-24
US20130122558A1 (en) 2013-05-16
BRPI0913714A2 (pt) 2020-10-13
AU2009293160B2 (en) 2013-11-21
WO2010033753A2 (en) 2010-03-25
CN102197047B (zh) 2014-07-09
JP5762291B2 (ja) 2015-08-12
US20100075386A1 (en) 2010-03-25
US20120309064A1 (en) 2012-12-06
JP2012502659A (ja) 2012-02-02
WO2010033753A3 (en) 2010-05-27
CN102197047A (zh) 2011-09-21
US8268598B2 (en) 2012-09-18
DK2324119T3 (en) 2016-07-18

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