CL2011000560A1 - Polipeptidos mutantes con actividad delta 5 desaturasa; acido nucleico y celula huesped que los comprenden, y metodo para producir acido araquidonico o acido eicosapentaenoico. - Google Patents

Polipeptidos mutantes con actividad delta 5 desaturasa; acido nucleico y celula huesped que los comprenden, y metodo para producir acido araquidonico o acido eicosapentaenoico.

Info

Publication number
CL2011000560A1
CL2011000560A1 CL2011000560A CL2011000560A CL2011000560A1 CL 2011000560 A1 CL2011000560 A1 CL 2011000560A1 CL 2011000560 A CL2011000560 A CL 2011000560A CL 2011000560 A CL2011000560 A CL 2011000560A CL 2011000560 A1 CL2011000560 A1 CL 2011000560A1
Authority
CL
Chile
Prior art keywords
acid
delta
host cell
nucleic acid
desaturase activity
Prior art date
Application number
CL2011000560A
Other languages
English (en)
Spanish (es)
Inventor
Qun Zhu
Dana M Walters Pollak
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of CL2011000560A1 publication Critical patent/CL2011000560A1/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CL2011000560A 2008-09-19 2011-03-17 Polipeptidos mutantes con actividad delta 5 desaturasa; acido nucleico y celula huesped que los comprenden, y metodo para producir acido araquidonico o acido eicosapentaenoico. CL2011000560A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US9833308P 2008-09-19 2008-09-19

Publications (1)

Publication Number Publication Date
CL2011000560A1 true CL2011000560A1 (es) 2011-10-07

Family

ID=42038064

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2011000560A CL2011000560A1 (es) 2008-09-19 2011-03-17 Polipeptidos mutantes con actividad delta 5 desaturasa; acido nucleico y celula huesped que los comprenden, y metodo para producir acido araquidonico o acido eicosapentaenoico.

Country Status (10)

Country Link
US (3) US8268598B2 (enExample)
EP (1) EP2324119B1 (enExample)
JP (1) JP5762291B2 (enExample)
CN (1) CN102197047B (enExample)
AU (1) AU2009293160B2 (enExample)
BR (1) BRPI0913714A2 (enExample)
CA (1) CA2735379A1 (enExample)
CL (1) CL2011000560A1 (enExample)
DK (1) DK2324119T3 (enExample)
WO (1) WO2010033753A2 (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2324119B1 (en) * 2008-09-19 2016-03-30 E. I. du Pont de Nemours and Company Mutant delta5 desaturases and their use in making polyunsaturated fatty acids
EP2561050A1 (en) 2010-04-22 2013-02-27 E.I. Du Pont De Nemours And Company Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass
US20140093910A1 (en) * 2010-06-10 2014-04-03 Bengurion University Of The Negev Research And Development Authority D5 desaturase-defective mutant gene and use thereof
CN103249834B (zh) 2010-08-26 2016-10-26 纳幕尔杜邦公司 生产高水平二十碳五烯酸的重组微生物宿主细胞
EP2609202A1 (en) * 2010-08-26 2013-07-03 E.I. Du Pont De Nemours And Company Mutant hpgg motif and hdash motif delta-5 desaturases and their use in making polyunsaturated fatty acids
US8980589B2 (en) 2010-08-26 2015-03-17 E I Du Pont De Nemours And Company Mutant delta-9 elongases and their use in making polyunsaturated fatty acids
JP2014503216A (ja) 2010-12-30 2014-02-13 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー スクロース利用のための、ヤロウィア・リポリティカ（Ｙａｒｒｏｗｉａｌｉｐｏｌｙｔｉｃａ）におけるサッカロミセス・セレビシア（Ｓａｃｃｈａｒｏｍｙｃｅｓｃｅｒｅｖｉｓｉａｅ）ＳＵＣ２遺伝子の使用
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
CN103841825B (zh) 2011-02-11 2017-03-22 纳幕尔杜邦公司 从微生物生物质中获取含脂质组合物的方法
CA2877279A1 (en) * 2012-06-19 2013-12-27 E. I. Du Pont De Nemours And Company Improved production of polyunsaturated fatty acids by coexpression of acyl-coa:lysophosphatidylcholine acyltransferases and phospholipid:diacylglycerol acyltransferases
CN102978177B (zh) * 2012-11-09 2015-06-03 浙江工业大学 冬虫夏草合成代谢二十碳五烯酸的△-5-脱氢酶、基因及应用
WO2014074770A2 (en) 2012-11-09 2014-05-15 Heliae Development, Llc Balanced mixotrophy methods
WO2014074772A1 (en) 2012-11-09 2014-05-15 Heliae Development, Llc Mixotrophic, phototrophic, and heterotrophic combination methods and systems
US9005940B2 (en) 2012-12-21 2015-04-14 E I Du Pont De Nemours And Company Down-regulation of a polynucleotide encoding a Sou2 sorbitol utilization protein to modify lipid production in microbial cells
JP2014193153A (ja) * 2013-02-28 2014-10-09 Euglena Co Ltd ユーグレナへの遺伝子導入方法
WO2014133159A1 (ja) * 2013-02-28 2014-09-04 株式会社ユーグレナ ユーグレナへの遺伝子導入方法及びその形質転換体
JP2014193154A (ja) * 2013-02-28 2014-10-09 Euglena Co Ltd ユーグレナの形質転換体
ES2915598T3 (es) 2018-12-12 2022-06-23 Nippon Suisan Kaisha Ltd Una composición que contiene un ácido graso altamente insaturado o un éster alquílico del mismo y un método para producirla

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5972664A (en) * 1997-04-11 1999-10-26 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
EP2166067B1 (de) * 2003-08-01 2015-07-01 BASF Plant Science GmbH Verfahren zur Herstellung mehrfach ungesättigter Fettsäuren in transgenen Organismen
US7678560B2 (en) * 2006-05-17 2010-03-16 E.I. Du Pont De Nemours And Company Δ 5 desaturase and its use in making polyunsaturated fatty acids
US7695950B2 (en) * 2006-05-17 2010-04-13 E. I. Du Pont De Nemours And Company Δ5 desaturase and its use in making polyunsaturated fatty acids
US8846374B2 (en) 2006-12-12 2014-09-30 E I Du Pont De Nemours And Company Carotenoid production in a recombinant oleaginous yeast
JP5711968B2 (ja) 2007-10-03 2015-05-07 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 高エイコサペンタエン酸を生成するためのヤロウィア・リポリティカ（ＹａｒｒｏｗｉａＬｉｐｏｌｙｔｉｃａ）の最適化株
EP2324119B1 (en) * 2008-09-19 2016-03-30 E. I. du Pont de Nemours and Company Mutant delta5 desaturases and their use in making polyunsaturated fatty acids

Also Published As

Publication number Publication date
DK2324119T3 (en) 2016-07-18
CN102197047B (zh) 2014-07-09
US20120309064A1 (en) 2012-12-06
JP5762291B2 (ja) 2015-08-12
US20100075386A1 (en) 2010-03-25
US20130122558A1 (en) 2013-05-16
BRPI0913714A2 (pt) 2020-10-13
AU2009293160B2 (en) 2013-11-21
EP2324119B1 (en) 2016-03-30
AU2009293160A1 (en) 2010-03-25
CN102197047A (zh) 2011-09-21
JP2012502659A (ja) 2012-02-02
WO2010033753A2 (en) 2010-03-25
US8614075B2 (en) 2013-12-24
US8268598B2 (en) 2012-09-18
CA2735379A1 (en) 2010-03-25
US8367383B2 (en) 2013-02-05
EP2324119A2 (en) 2011-05-25
WO2010033753A3 (en) 2010-05-27
EP2324119A4 (en) 2013-05-01

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