AU2009293160B2 - Mutant delta5 desaturases and their use in making polyunsaturated fatty acids - Google Patents

Mutant delta5 desaturases and their use in making polyunsaturated fatty acids Download PDF

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Publication number
AU2009293160B2
AU2009293160B2 AU2009293160A AU2009293160A AU2009293160B2 AU 2009293160 B2 AU2009293160 B2 AU 2009293160B2 AU 2009293160 A AU2009293160 A AU 2009293160A AU 2009293160 A AU2009293160 A AU 2009293160A AU 2009293160 B2 AU2009293160 B2 AU 2009293160B2
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AU
Australia
Prior art keywords
seq
mutant
desaturase
amino acid
host cell
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Ceased
Application number
AU2009293160A
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English (en)
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AU2009293160A1 (en
Inventor
Dana M. Walters Pollak
Qun Zhu
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EIDP Inc
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EI Du Pont de Nemours and Co
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Publication of AU2009293160A1 publication Critical patent/AU2009293160A1/en
Application granted granted Critical
Publication of AU2009293160B2 publication Critical patent/AU2009293160B2/en
Ceased legal-status Critical Current
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
AU2009293160A 2008-09-19 2009-09-18 Mutant delta5 desaturases and their use in making polyunsaturated fatty acids Ceased AU2009293160B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US9833308P 2008-09-19 2008-09-19
US61/098,333 2008-09-19
PCT/US2009/057393 WO2010033753A2 (en) 2008-09-19 2009-09-18 Mutant delta5 desaturases and their use in making polyunsaturated fatty acids

Publications (2)

Publication Number Publication Date
AU2009293160A1 AU2009293160A1 (en) 2010-03-25
AU2009293160B2 true AU2009293160B2 (en) 2013-11-21

Family

ID=42038064

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2009293160A Ceased AU2009293160B2 (en) 2008-09-19 2009-09-18 Mutant delta5 desaturases and their use in making polyunsaturated fatty acids

Country Status (10)

Country Link
US (3) US8268598B2 (enExample)
EP (1) EP2324119B1 (enExample)
JP (1) JP5762291B2 (enExample)
CN (1) CN102197047B (enExample)
AU (1) AU2009293160B2 (enExample)
BR (1) BRPI0913714A2 (enExample)
CA (1) CA2735379A1 (enExample)
CL (1) CL2011000560A1 (enExample)
DK (1) DK2324119T3 (enExample)
WO (1) WO2010033753A2 (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2010033753A2 (en) * 2008-09-19 2010-03-25 E. I. Du Pont De Nemours And Company Mutant delta5 desaturases and their use in making polyunsaturated fatty acids
EP2561050A1 (en) 2010-04-22 2013-02-27 E.I. Du Pont De Nemours And Company Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass
WO2011154947A2 (en) * 2010-06-10 2011-12-15 Ben-Gurion University Of The Negev Research And Development Authority D5 desaturase-defective mutant gene and use thereof
JP2013535988A (ja) * 2010-08-26 2013-09-19 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 変異ｈｐｇｇモチーフおよびｈｄａｓｈモチーフδ５デサチュラーゼおよび多価不飽和脂肪酸の製造におけるそれらの使用
CA2807834C (en) 2010-08-26 2019-06-25 E. I. Du Pont De Nemours And Company Recombinant microbial host cells for high eicosapentaenoic acid production
JP5963749B2 (ja) 2010-08-26 2016-08-03 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 変異δ９エロンガーゼおよび多価不飽和脂肪酸の製造におけるそれらの使用
AU2011353002A1 (en) 2010-12-30 2013-05-30 E. I. Du Pont De Nemours And Company Use of Saccharomyces cerevisiae SUC2 gene in Yarrowia lipolytica for sucrose utilization
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
CA2825039C (en) 2011-02-11 2020-04-28 E.I. Du Pont De Nemours And Company Method for obtaining a lipid-containing composition from microbial biomass
WO2013192002A1 (en) * 2012-06-19 2013-12-27 E. I. Du Pont De Nemours And Company IMPROVED PRODUCTION OF POLYUNSATURATED FATTY ACIDS BY COEXPRESSION OF ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES AND PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASES
CN102978177B (zh) * 2012-11-09 2015-06-03 浙江工业大学 冬虫夏草合成代谢二十碳五烯酸的△-5-脱氢酶、基因及应用
WO2014074772A1 (en) 2012-11-09 2014-05-15 Heliae Development, Llc Mixotrophic, phototrophic, and heterotrophic combination methods and systems
WO2014074770A2 (en) 2012-11-09 2014-05-15 Heliae Development, Llc Balanced mixotrophy methods
WO2014100061A1 (en) 2012-12-21 2014-06-26 E. I. Du Pont De Nemours And Company Down-regulation of a polynucleotide encoding a sou2 sorbitol utilization protein to modify lipid production in microbial cells
WO2014133159A1 (ja) * 2013-02-28 2014-09-04 株式会社ユーグレナ ユーグレナへの遺伝子導入方法及びその形質転換体
JP2014193154A (ja) * 2013-02-28 2014-10-09 Euglena Co Ltd ユーグレナの形質転換体
JP2014193153A (ja) * 2013-02-28 2014-10-09 Euglena Co Ltd ユーグレナへの遺伝子導入方法
EP3666082B1 (en) 2018-12-12 2022-05-04 Nippon Suisan Kaisha, Ltd. A composition containing highly unsaturated fatty acid or alkyl ester thereof and a method for producing the same

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007136671A2 (en) * 2006-05-17 2007-11-29 E. I. Du Pont De Nemours And Company Delta5 desaturase and its use in making polyunsaturated fatty acids
WO2009046231A1 (en) * 2007-10-03 2009-04-09 E. I. Du Pont De Nemours And Company Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5972664A (en) 1997-04-11 1999-10-26 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
NO347177B1 (no) * 2003-08-01 2023-06-19 Basf Plant Science Gmbh Fremgangsmåte for fremstilling av flerumettede fettsyrer i transgene organismer
US7695950B2 (en) * 2006-05-17 2010-04-13 E. I. Du Pont De Nemours And Company Δ5 desaturase and its use in making polyunsaturated fatty acids
US8846374B2 (en) 2006-12-12 2014-09-30 E I Du Pont De Nemours And Company Carotenoid production in a recombinant oleaginous yeast
WO2010033753A2 (en) * 2008-09-19 2010-03-25 E. I. Du Pont De Nemours And Company Mutant delta5 desaturases and their use in making polyunsaturated fatty acids

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007136671A2 (en) * 2006-05-17 2007-11-29 E. I. Du Pont De Nemours And Company Delta5 desaturase and its use in making polyunsaturated fatty acids
WO2009046231A1 (en) * 2007-10-03 2009-04-09 E. I. Du Pont De Nemours And Company Optimized strains of yarrowia lipolytica for high eicosapentaenoic acid production

Also Published As

Publication number Publication date
CN102197047B (zh) 2014-07-09
EP2324119A4 (en) 2013-05-01
US8614075B2 (en) 2013-12-24
EP2324119B1 (en) 2016-03-30
US20100075386A1 (en) 2010-03-25
BRPI0913714A2 (pt) 2020-10-13
JP2012502659A (ja) 2012-02-02
US8268598B2 (en) 2012-09-18
CN102197047A (zh) 2011-09-21
DK2324119T3 (en) 2016-07-18
US20130122558A1 (en) 2013-05-16
CL2011000560A1 (es) 2011-10-07
AU2009293160A1 (en) 2010-03-25
WO2010033753A3 (en) 2010-05-27
US8367383B2 (en) 2013-02-05
US20120309064A1 (en) 2012-12-06
WO2010033753A2 (en) 2010-03-25
CA2735379A1 (en) 2010-03-25
JP5762291B2 (ja) 2015-08-12
EP2324119A2 (en) 2011-05-25

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FGA Letters patent sealed or granted (standard patent)
MK14 Patent ceased section 143(a) (annual fees not paid) or expired