CN102197047B - 突变的δ5去饱和酶及其在制备多不饱和脂肪酸中的用途 - Google Patents

突变的δ5去饱和酶及其在制备多不饱和脂肪酸中的用途 Download PDF

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Publication number
CN102197047B
CN102197047B CN200980136806.0A CN200980136806A CN102197047B CN 102197047 B CN102197047 B CN 102197047B CN 200980136806 A CN200980136806 A CN 200980136806A CN 102197047 B CN102197047 B CN 102197047B
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desaturase
seq
acid
host cell
mutant
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Expired - Fee Related
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Chinese (zh)
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CN102197047A (zh
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Q·朱
D·M·W·波拉克
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EIDP Inc
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EI Du Pont de Nemours and Co
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CN200980136806.0A 2008-09-19 2009-09-18 突变的δ5去饱和酶及其在制备多不饱和脂肪酸中的用途 Expired - Fee Related CN102197047B (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US9833308P 2008-09-19 2008-09-19
US61/098333 2008-09-19
US61/098,333 2008-09-19
PCT/US2009/057393 WO2010033753A2 (en) 2008-09-19 2009-09-18 Mutant delta5 desaturases and their use in making polyunsaturated fatty acids

Publications (2)

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CN102197047A CN102197047A (zh) 2011-09-21
CN102197047B true CN102197047B (zh) 2014-07-09

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CN200980136806.0A Expired - Fee Related CN102197047B (zh) 2008-09-19 2009-09-18 突变的δ5去饱和酶及其在制备多不饱和脂肪酸中的用途

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US (3) US8268598B2 (enExample)
EP (1) EP2324119B1 (enExample)
JP (1) JP5762291B2 (enExample)
CN (1) CN102197047B (enExample)
AU (1) AU2009293160B2 (enExample)
BR (1) BRPI0913714A2 (enExample)
CA (1) CA2735379A1 (enExample)
CL (1) CL2011000560A1 (enExample)
DK (1) DK2324119T3 (enExample)
WO (1) WO2010033753A2 (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2324119T3 (en) * 2008-09-19 2016-07-18 Du Pont Mutant DELTA5 Desaturases AND USE THEREOF FOR THE PRODUCTION OF polyunsaturated fatty acids
WO2011133610A1 (en) 2010-04-22 2011-10-27 E. I. Du Pont De Nemours And Company Method for obtaining polyunsaturated fatty acid-containing compositions from microbial biomass
US20140093910A1 (en) * 2010-06-10 2014-04-03 Bengurion University Of The Negev Research And Development Authority D5 desaturase-defective mutant gene and use thereof
CA2808002A1 (en) * 2010-08-26 2012-03-01 E. I. Du Pont De Nemours And Company Mutant hpgg motif and hdash motif delta-5 desaturases and their use in making polyunsaturated fatty acids
DK2609203T3 (da) 2010-08-26 2018-10-01 Du Pont Rekombinante mikrobielle værtsceller til større fremstilling af eicosapentaensyre
WO2012027676A1 (en) 2010-08-26 2012-03-01 E. I. Du Pont De Nemours And Company Mutant delta-9 elongases and their use in making polyunsaturated fatty acids
KR20140000711A (ko) 2010-12-30 2014-01-03 이 아이 듀폰 디 네모아 앤드 캄파니 수크로스 이용에 있어서의 야로위아 리포라이티카에서의 사카로마이세스 세레비지애 suc2 유전자의 용도
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
CA3026436A1 (en) 2011-02-11 2012-08-16 E. I. Du Pont De Nemours And Company Method for obtaining a lipid-containing composition from microbial biomass
BR112014031784A2 (pt) * 2012-06-19 2019-10-01 Du Pont acil-coa:lisofosfatidilcolina aciltransferases mutantes
WO2014074770A2 (en) 2012-11-09 2014-05-15 Heliae Development, Llc Balanced mixotrophy methods
WO2014074772A1 (en) 2012-11-09 2014-05-15 Heliae Development, Llc Mixotrophic, phototrophic, and heterotrophic combination methods and systems
CN102978177B (zh) * 2012-11-09 2015-06-03 浙江工业大学 冬虫夏草合成代谢二十碳五烯酸的△-5-脱氢酶、基因及应用
CA2892516A1 (en) 2012-12-21 2014-06-26 E. I. Du Pont De Nemours And Company Down-regulation of a polynucleotide encoding a sou2 sorbitol utilization protein to modify lipid production in microbial cells
JP2014193154A (ja) * 2013-02-28 2014-10-09 Euglena Co Ltd ユーグレナの形質転換体
WO2014133159A1 (ja) * 2013-02-28 2014-09-04 株式会社ユーグレナ ユーグレナへの遺伝子導入方法及びその形質転換体
JP2014193153A (ja) * 2013-02-28 2014-10-09 Euglena Co Ltd ユーグレナへの遺伝子導入方法
EP3666082B1 (en) 2018-12-12 2022-05-04 Nippon Suisan Kaisha, Ltd. A composition containing highly unsaturated fatty acid or alkyl ester thereof and a method for producing the same

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20070271632A1 (en) * 2006-05-17 2007-11-22 Howard Glenn Damude Delta 5 desaturase and its use in making polyunsaturated fatty acids
US20080155705A1 (en) * 2003-08-01 2008-06-26 Basf Plant Science Gmbh Method For the Production of Multiple-Unsaturated Fatty Acids in Transgenic Organisms

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5972664A (en) * 1997-04-11 1999-10-26 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
US7678560B2 (en) * 2006-05-17 2010-03-16 E.I. Du Pont De Nemours And Company Δ 5 desaturase and its use in making polyunsaturated fatty acids
US8846374B2 (en) 2006-12-12 2014-09-30 E I Du Pont De Nemours And Company Carotenoid production in a recombinant oleaginous yeast
US8323935B2 (en) 2007-10-03 2012-12-04 E I Du Pont De Nemours And Company Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production
DK2324119T3 (en) * 2008-09-19 2016-07-18 Du Pont Mutant DELTA5 Desaturases AND USE THEREOF FOR THE PRODUCTION OF polyunsaturated fatty acids

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20080155705A1 (en) * 2003-08-01 2008-06-26 Basf Plant Science Gmbh Method For the Production of Multiple-Unsaturated Fatty Acids in Transgenic Organisms
US20070271632A1 (en) * 2006-05-17 2007-11-22 Howard Glenn Damude Delta 5 desaturase and its use in making polyunsaturated fatty acids

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
The alternative pathway C20 D8-desaturase from the non-photosynthetic organism Acanthamoeba castellanii is an atypical cytochrome b5-fusion desaturase.;SAYANOVA等;《FEBS》;20061231;第580卷;1946-1952 *
肖靓等.实时PCR分析Δ5脱饱和酶在花生四烯酸合成中的作用.《中国生物工程杂志》.2006,第26卷(第8期),57-61. *

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Publication number Publication date
US8367383B2 (en) 2013-02-05
EP2324119B1 (en) 2016-03-30
BRPI0913714A2 (pt) 2020-10-13
CN102197047A (zh) 2011-09-21
CL2011000560A1 (es) 2011-10-07
US20120309064A1 (en) 2012-12-06
CA2735379A1 (en) 2010-03-25
JP2012502659A (ja) 2012-02-02
JP5762291B2 (ja) 2015-08-12
AU2009293160A1 (en) 2010-03-25
EP2324119A4 (en) 2013-05-01
US20100075386A1 (en) 2010-03-25
WO2010033753A2 (en) 2010-03-25
US20130122558A1 (en) 2013-05-16
DK2324119T3 (en) 2016-07-18
AU2009293160B2 (en) 2013-11-21
US8614075B2 (en) 2013-12-24
WO2010033753A3 (en) 2010-05-27
US8268598B2 (en) 2012-09-18
EP2324119A2 (en) 2011-05-25

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