JP5762291B2 - 変異δ５デサチュラーゼと、多価不飽和脂肪酸の製造におけるそれらの使用 - Google Patents

変異δ５デサチュラーゼと、多価不飽和脂肪酸の製造におけるそれらの使用 Download PDF

Info

Publication number
JP5762291B2
JP5762291B2 JP2011527976A JP2011527976A JP5762291B2 JP 5762291 B2 JP5762291 B2 JP 5762291B2 JP 2011527976 A JP2011527976 A JP 2011527976A JP 2011527976 A JP2011527976 A JP 2011527976A JP 5762291 B2 JP5762291 B2 JP 5762291B2
Authority
JP
Japan
Prior art keywords
desaturase
seq
acid
gene
mutant
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
JP2011527976A
Other languages
English (en)
Japanese (ja)
Other versions
JP2012502659A5 (enExample
JP2012502659A (ja
Inventor
クン ズー
クン ズー
ダナ エム ウォルターズ ポラック
ダナ エム ウォルターズ ポラック
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of JP2012502659A publication Critical patent/JP2012502659A/ja
Publication of JP2012502659A5 publication Critical patent/JP2012502659A5/ja
Application granted granted Critical
Publication of JP5762291B2 publication Critical patent/JP5762291B2/ja
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
    • C12P7/6432—Eicosapentaenoic acids [EPA]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
JP2011527976A 2008-09-19 2009-09-18 変異δ５デサチュラーゼと、多価不飽和脂肪酸の製造におけるそれらの使用 Expired - Fee Related JP5762291B2 (ja)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US9833308P 2008-09-19 2008-09-19
US61/098,333 2008-09-19
PCT/US2009/057393 WO2010033753A2 (en) 2008-09-19 2009-09-18 Mutant delta5 desaturases and their use in making polyunsaturated fatty acids

Publications (3)

Publication Number Publication Date
JP2012502659A JP2012502659A (ja) 2012-02-02
JP2012502659A5 JP2012502659A5 (enExample) 2012-10-11
JP5762291B2 true JP5762291B2 (ja) 2015-08-12

Family

ID=42038064

Family Applications (1)

Application Number Title Priority Date Filing Date
JP2011527976A Expired - Fee Related JP5762291B2 (ja) 2008-09-19 2009-09-18 変異δ５デサチュラーゼと、多価不飽和脂肪酸の製造におけるそれらの使用

Country Status (10)

Country Link
US (3) US8268598B2 (enExample)
EP (1) EP2324119B1 (enExample)
JP (1) JP5762291B2 (enExample)
CN (1) CN102197047B (enExample)
AU (1) AU2009293160B2 (enExample)
BR (1) BRPI0913714A2 (enExample)
CA (1) CA2735379A1 (enExample)
CL (1) CL2011000560A1 (enExample)
DK (1) DK2324119T3 (enExample)
WO (1) WO2010033753A2 (enExample)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2324119T3 (en) * 2008-09-19 2016-07-18 Du Pont Mutant DELTA5 Desaturases AND USE THEREOF FOR THE PRODUCTION OF polyunsaturated fatty acids
CN103282474A (zh) 2010-04-22 2013-09-04 纳幕尔杜邦公司 从微生物生物质中获得包含多不饱和脂肪酸的组合物的方法
WO2011154947A2 (en) * 2010-06-10 2011-12-15 Ben-Gurion University Of The Negev Research And Development Authority D5 desaturase-defective mutant gene and use thereof
AU2011293189B2 (en) * 2010-08-26 2017-02-16 E. I. Du Pont De Nemours And Company Mutant HPGG motif and HDASH motif delta-5 desaturases and their use in making polyunsaturated fatty acids
US8703473B2 (en) 2010-08-26 2014-04-22 E I Du Pont De Nemours And Company Recombinant microbial host cells for high eicosapentaenoic acid production
WO2012027676A1 (en) 2010-08-26 2012-03-01 E. I. Du Pont De Nemours And Company Mutant delta-9 elongases and their use in making polyunsaturated fatty acids
BR112013016769A2 (pt) 2010-12-30 2016-10-11 Du Pont yarrowia lipolytica transformada e método para produzir pelo menos um produto não nativo de interesse
US20130040340A1 (en) 2011-02-07 2013-02-14 E. I. Du Pont De Nemours And Company Production of alcohol esters in situ using alcohols and fatty acids produced by microorganisms
JP6061866B2 (ja) 2011-02-11 2017-01-18 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 微生物バイオマスから脂質含有組成物を得る方法
WO2013192007A1 (en) * 2012-06-19 2013-12-27 E. I. Du Pont De Nemours And Company MUTANT ACYL-CoA:LYSOPHOSPHATIDYLCHOLINE ACYLTRANSFERASES
CN102978177B (zh) * 2012-11-09 2015-06-03 浙江工业大学 冬虫夏草合成代谢二十碳五烯酸的△-5-脱氢酶、基因及应用
WO2014074772A1 (en) 2012-11-09 2014-05-15 Heliae Development, Llc Mixotrophic, phototrophic, and heterotrophic combination methods and systems
WO2014074770A2 (en) 2012-11-09 2014-05-15 Heliae Development, Llc Balanced mixotrophy methods
CA2892516A1 (en) 2012-12-21 2014-06-26 E. I. Du Pont De Nemours And Company Down-regulation of a polynucleotide encoding a sou2 sorbitol utilization protein to modify lipid production in microbial cells
US20160010070A1 (en) * 2013-02-28 2016-01-14 Kinki University Method for introducing gene to euglena, and transformant therefrom
JP2014193153A (ja) * 2013-02-28 2014-10-09 Euglena Co Ltd ユーグレナへの遺伝子導入方法
JP2014193154A (ja) * 2013-02-28 2014-10-09 Euglena Co Ltd ユーグレナの形質転換体
EP3666082B1 (en) 2018-12-12 2022-05-04 Nippon Suisan Kaisha, Ltd. A composition containing highly unsaturated fatty acid or alkyl ester thereof and a method for producing the same

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5972664A (en) * 1997-04-11 1999-10-26 Abbott Laboratories Methods and compositions for synthesis of long chain poly-unsaturated fatty acids
US7238482B2 (en) 2003-05-07 2007-07-03 E. I. Du Pont De Nemours And Company Production of polyunsaturated fatty acids in oleaginous yeasts
PT2172536T (pt) * 2003-08-01 2016-08-17 Basf Plant Science Gmbh Processo para a produção de ácidos gordos poli-insaturados em organismos transgénicos
US7678560B2 (en) * 2006-05-17 2010-03-16 E.I. Du Pont De Nemours And Company Δ 5 desaturase and its use in making polyunsaturated fatty acids
US7695950B2 (en) * 2006-05-17 2010-04-13 E. I. Du Pont De Nemours And Company Δ5 desaturase and its use in making polyunsaturated fatty acids
US8846374B2 (en) 2006-12-12 2014-09-30 E I Du Pont De Nemours And Company Carotenoid production in a recombinant oleaginous yeast
US8323935B2 (en) 2007-10-03 2012-12-04 E I Du Pont De Nemours And Company Optimized strains of Yarrowia lipolytica for high eicosapentaenoic acid production
DK2324119T3 (en) * 2008-09-19 2016-07-18 Du Pont Mutant DELTA5 Desaturases AND USE THEREOF FOR THE PRODUCTION OF polyunsaturated fatty acids

Also Published As

Publication number Publication date
BRPI0913714A2 (pt) 2020-10-13
CL2011000560A1 (es) 2011-10-07
AU2009293160B2 (en) 2013-11-21
US8367383B2 (en) 2013-02-05
DK2324119T3 (en) 2016-07-18
CA2735379A1 (en) 2010-03-25
EP2324119A2 (en) 2011-05-25
EP2324119A4 (en) 2013-05-01
US20100075386A1 (en) 2010-03-25
AU2009293160A1 (en) 2010-03-25
US8268598B2 (en) 2012-09-18
US8614075B2 (en) 2013-12-24
WO2010033753A2 (en) 2010-03-25
JP2012502659A (ja) 2012-02-02
CN102197047A (zh) 2011-09-21
US20120309064A1 (en) 2012-12-06
EP2324119B1 (en) 2016-03-30
WO2010033753A3 (en) 2010-05-27
CN102197047B (zh) 2014-07-09
US20130122558A1 (en) 2013-05-16

Similar Documents

Publication Publication Date Title
JP5762291B2 (ja) 変異δ５デサチュラーゼと、多価不飽和脂肪酸の製造におけるそれらの使用
JP5193216B2 (ja) Δ１７デサチュラーゼおよび多価不飽和脂肪酸の製造におけるその使用
US7790156B2 (en) Δ-8 desaturases and their use in making polyunsaturated fatty acids
JP5572620B2 (ja) Δ４デサチュラーゼ、および多価不飽和脂肪酸の製造におけるその使用
EP2010648B1 (en) Delta-17 desaturases and their use in making polyunsaturated fatty acids
WO2008073271A9 (en) Mutant delta8 desaturase genes engineered by targeted mutagenesis and their use in making polyunsaturated fatty acids
JP5963749B2 (ja) 変異δ９エロンガーゼおよび多価不飽和脂肪酸の製造におけるそれらの使用
US8658413B2 (en) Mutant HPGG motif and HDASH motif delta-5 desaturases and their use in making polyunsaturated fatty acids
JP2011529689A (ja) Δ６デサチュラーゼおよび多価不飽和脂肪酸生成におけるそれらの使用

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20120827

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20120827

A131 Notification of reasons for refusal

Free format text: JAPANESE INTERMEDIATE CODE: A131

Effective date: 20140108

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20140313

A02 Decision of refusal

Free format text: JAPANESE INTERMEDIATE CODE: A02

Effective date: 20141001

A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20150202

A911 Transfer to examiner for re-examination before appeal (zenchi)

Free format text: JAPANESE INTERMEDIATE CODE: A911

Effective date: 20150316

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20150511

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20150609

R150 Certificate of patent or registration of utility model

Ref document number: 5762291

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

R250 Receipt of annual fees

Free format text: JAPANESE INTERMEDIATE CODE: R250

LAPS Cancellation because of no payment of annual fees