CL2009000912A1 - Metodo para la produccion de bmp-2 que comprende; cultivar una celula huesped que comprende la molecula de adn que codifica bmp-2 en medio de cultivo en presencia de hierro, un compuesto polianionico y piridoxal; recuperar la proteina de interes donde la celula huesped se cultiva en bioreactor. - Google Patents

Metodo para la produccion de bmp-2 que comprende; cultivar una celula huesped que comprende la molecula de adn que codifica bmp-2 en medio de cultivo en presencia de hierro, un compuesto polianionico y piridoxal; recuperar la proteina de interes donde la celula huesped se cultiva en bioreactor.

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Publication number
CL2009000912A1
CL2009000912A1 CL2009000912A CL2009000912A CL2009000912A1 CL 2009000912 A1 CL2009000912 A1 CL 2009000912A1 CL 2009000912 A CL2009000912 A CL 2009000912A CL 2009000912 A CL2009000912 A CL 2009000912A CL 2009000912 A1 CL2009000912 A1 CL 2009000912A1
Authority
CL
Chile
Prior art keywords
bmp
host cell
production
pyridoxal
iron
Prior art date
Application number
CL2009000912A
Other languages
English (en)
Inventor
Denis Drapeau
Wenge Wang
Jose Manuel Gomes
Yen-Tung Luan
Gregg Nyberg
Terry Cardoza
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Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=40740265&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=CL2009000912(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed filed Critical
Publication of CL2009000912A1 publication Critical patent/CL2009000912A1/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • A61P19/08—Drugs for skeletal disorders for bone diseases, e.g. rachitism, Paget's disease
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/51—Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C12N5/0037—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2500/00—Specific components of cell culture medium
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    • C12N2500/00—Specific components of cell culture medium
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/90—Polysaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Cell Biology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Tropical Medicine & Parasitology (AREA)
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  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Rheumatology (AREA)
  • Reproductive Health (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)

Abstract

SE PROPORCIONAN MÉTODOS Y PROCESOS PARA LA PRODUCCIÓN DE PROTEÍNA RECOMBINANTE MEJORADA. LOS MÉTODOS SON ÚTILES PARA LA PRODUCCIÓN DE FACTORES DE CRECIMIENTO, EN PARTICULAR LOS DE LA SUPERFAMILIA DE TGF-B, QUE INCLUYEN PROTEÍNAS MORFOGENÉTICAS ÓSEAS (BMP), TALES COMO BMP-2. LAS CÉLULAS HUÉSPED ADECUADAS SE CULTIVAN EN UN MEDIO EN DONDE EL HIERRO ESTÁ PRESENTE EN UNA CONCENTRACIÓN DE AL MENOS 2,25 UM Y SI EL PIRIDOXAL ESTÁ PRESENTE, CONSTITUYE MENOS DE 55% DE LA CONCENTRACIÓN MOLAR DE VITAMINA B6 DEL MEDIO.
CL2009000912A 2008-04-17 2009-04-16 Metodo para la produccion de bmp-2 que comprende; cultivar una celula huesped que comprende la molecula de adn que codifica bmp-2 en medio de cultivo en presencia de hierro, un compuesto polianionico y piridoxal; recuperar la proteina de interes donde la celula huesped se cultiva en bioreactor. CL2009000912A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US4564308P 2008-04-17 2008-04-17

Publications (1)

Publication Number Publication Date
CL2009000912A1 true CL2009000912A1 (es) 2009-10-09

Family

ID=40740265

Family Applications (1)

Application Number Title Priority Date Filing Date
CL2009000912A CL2009000912A1 (es) 2008-04-17 2009-04-16 Metodo para la produccion de bmp-2 que comprende; cultivar una celula huesped que comprende la molecula de adn que codifica bmp-2 en medio de cultivo en presencia de hierro, un compuesto polianionico y piridoxal; recuperar la proteina de interes donde la celula huesped se cultiva en bioreactor.

Country Status (19)

Country Link
US (1) US8039231B2 (es)
EP (1) EP2310523B1 (es)
JP (2) JP5739324B2 (es)
KR (1) KR101286895B1 (es)
CN (2) CN102037132A (es)
AR (2) AR071484A1 (es)
AU (1) AU2009236187B2 (es)
CA (1) CA2720980C (es)
CL (1) CL2009000912A1 (es)
DK (1) DK2310523T3 (es)
ES (1) ES2543061T3 (es)
HU (1) HUE027035T2 (es)
IL (1) IL208732A (es)
MX (1) MX2010011356A (es)
PL (1) PL2310523T3 (es)
PT (1) PT2310523E (es)
SI (1) SI2310523T1 (es)
WO (1) WO2009129379A1 (es)
ZA (1) ZA201007099B (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101286895B1 (ko) * 2008-04-17 2013-07-16 와이어쓰 엘엘씨 골 형태발생 단백질의 개선된 생산 방법
US9809635B2 (en) * 2010-03-19 2017-11-07 Lifenet Health BMP-2 peptides and methods of use
WO2011119833A2 (en) * 2010-03-24 2011-09-29 Lifenet Health Bmp-7 peptides & methods of use
US11078248B2 (en) 2010-03-19 2021-08-03 Lifenet Health BMP peptides and methods of use
US9951112B2 (en) * 2010-03-24 2018-04-24 Lifenet Health BMP-4 peptides and methods of use
JP5953502B2 (ja) 2010-07-08 2016-07-20 バクスアルタ ゲーエムベーハー 細胞培養による組換え型高分子量ｖＷＦ産生方法
US20130045471A1 (en) * 2011-02-25 2013-02-21 Bio-Rad Laboratories, Inc. Training system for investigations of bioengineered proteins
TW201247874A (en) * 2011-05-25 2012-12-01 United Biomedical Inc Method for producing the activity of bone activating protein
US20130281355A1 (en) * 2012-04-24 2013-10-24 Genentech, Inc. Cell culture compositions and methods for polypeptide production
EP3683316A1 (en) * 2014-01-30 2020-07-22 Coherus Biosciences, Inc. Perfusion media
WO2018194413A1 (en) * 2017-04-21 2018-10-25 Yuhan Corporation Method for producing dual function proteins and its derivatives
KR102056075B1 (ko) * 2018-06-05 2020-01-22 한국과학기술원 목적 단백질의 생산을 향상시키기 위한 동물세포 배양용 배지 조성물 및 이를 이용한 목적 단백질 생산 방법

Family Cites Families (12)

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US5013649A (en) 1986-07-01 1991-05-07 Genetics Institute, Inc. DNA sequences encoding osteoinductive products
US5318898A (en) * 1991-04-02 1994-06-07 Genetics Institute, Inc. Production of recombinant bone-inducing proteins
US5830761A (en) 1995-06-07 1998-11-03 Genetics Institute, Inc. Medium and methods for culturing mammalian cho cells
US20030036629A1 (en) 1997-12-12 2003-02-20 Barry Foster Novel tgf-beta protein purification methods
WO2002077006A1 (en) 2001-03-23 2002-10-03 Human Genome Sciences, Inc. Bone morphogenic protein polynucleotides, polypeptides, and antibodies
EP1404813A4 (en) 2001-06-13 2004-11-24 Genentech Inc METHOD FOR CULTIVATING ANIMAL CELLS AND POLYPEPTIDE PRODUCTION IN ANIMAL CELLS
EP1623019B2 (en) 2003-05-15 2017-01-25 Wyeth LLC Restricted glucose feed for animal cell culture
US7300773B2 (en) * 2004-08-27 2007-11-27 Wyeth Research Ireland Limited Production of TNFR-Ig
TWI374935B (en) * 2004-08-27 2012-10-21 Pfizer Ireland Pharmaceuticals Production of α-abeta
US7294484B2 (en) * 2004-08-27 2007-11-13 Wyeth Research Ireland Limited Production of polypeptides
DE602005026548D1 (de) * 2004-11-02 2011-04-07 Ares Trading Sa Serumfreies kulturmedium für säugerzellen
KR101286895B1 (ko) * 2008-04-17 2013-07-16 와이어쓰 엘엘씨 골 형태발생 단백질의 개선된 생산 방법

Also Published As

Publication number Publication date
KR101286895B1 (ko) 2013-07-16
CN102037132A (zh) 2011-04-27
US20090317867A1 (en) 2009-12-24
EP2310523B1 (en) 2015-06-10
AU2009236187A1 (en) 2009-10-22
JP2015109854A (ja) 2015-06-18
US8039231B2 (en) 2011-10-18
CA2720980C (en) 2014-02-25
AR109907A2 (es) 2019-02-06
DK2310523T3 (en) 2015-07-20
IL208732A (en) 2014-07-31
JP2011518175A (ja) 2011-06-23
WO2009129379A1 (en) 2009-10-22
MX2010011356A (es) 2010-11-09
KR20110005874A (ko) 2011-01-19
AR071484A1 (es) 2010-06-23
ZA201007099B (en) 2011-06-29
PL2310523T3 (pl) 2015-10-30
SI2310523T1 (sl) 2015-10-30
HUE027035T2 (hu) 2016-08-29
JP5739324B2 (ja) 2015-06-24
CN107090481A (zh) 2017-08-25
CA2720980A1 (en) 2009-10-22
ES2543061T3 (es) 2015-08-14
AU2009236187B2 (en) 2012-08-02
IL208732A0 (en) 2010-12-30
EP2310523A1 (en) 2011-04-20
PT2310523E (pt) 2015-09-07

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