WO2011078667A3 - Method of finding a biocatalyst having ammonia lyase activity - Google Patents

Method of finding a biocatalyst having ammonia lyase activity Download PDF

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Publication number
WO2011078667A3
WO2011078667A3 PCT/NL2010/050876 NL2010050876W WO2011078667A3 WO 2011078667 A3 WO2011078667 A3 WO 2011078667A3 NL 2010050876 W NL2010050876 W NL 2010050876W WO 2011078667 A3 WO2011078667 A3 WO 2011078667A3
Authority
WO
WIPO (PCT)
Prior art keywords
ammonia lyase
lyase activity
biocatalyst
finding
cells
Prior art date
Application number
PCT/NL2010/050876
Other languages
French (fr)
Other versions
WO2011078667A2 (en
Inventor
Petronella Catharina Raemakers-Franken
Guido Meurer
Jürgen Wolfgang ECK
Klaus Jürgen LIEBETON
Axel Christoph Trefzer
Original Assignee
Dsm Ip Assets B.V.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets B.V. filed Critical Dsm Ip Assets B.V.
Publication of WO2011078667A2 publication Critical patent/WO2011078667A2/en
Publication of WO2011078667A3 publication Critical patent/WO2011078667A3/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/527—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving lyase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C229/00—Compounds containing amino and carboxyl groups bound to the same carbon skeleton
    • C07C229/02—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton
    • C07C229/04—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated
    • C07C229/06—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having only one amino and one carboxyl group bound to the carbon skeleton
    • C07C229/08—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having only one amino and one carboxyl group bound to the carbon skeleton the nitrogen atom of the amino group being further bound to hydrogen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C229/00—Compounds containing amino and carboxyl groups bound to the same carbon skeleton
    • C07C229/02—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton
    • C07C229/04—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated
    • C07C229/26—Compounds containing amino and carboxyl groups bound to the same carbon skeleton having amino and carboxyl groups bound to acyclic carbon atoms of the same carbon skeleton the carbon skeleton being acyclic and saturated having more than one amino group bound to the carbon skeleton, e.g. lysine
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07C—ACYCLIC OR CARBOCYCLIC COMPOUNDS
    • C07C233/00—Carboxylic acid amides
    • C07C233/01—Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms
    • C07C233/45—Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by carboxyl groups
    • C07C233/46—Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by carboxyl groups with the substituted hydrocarbon radical bound to the nitrogen atom of the carboxamide group by an acyclic carbon atom
    • C07C233/47—Carboxylic acid amides having carbon atoms of carboxamide groups bound to hydrogen atoms or to acyclic carbon atoms having the nitrogen atom of at least one of the carboxamide groups bound to a carbon atom of a hydrocarbon radical substituted by carboxyl groups with the substituted hydrocarbon radical bound to the nitrogen atom of the carboxamide group by an acyclic carbon atom having the carbon atom of the carboxamide group bound to a hydrogen atom or to a carbon atom of an acyclic saturated carbon skeleton
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/005—Amino acids other than alpha- or beta amino acids, e.g. gamma amino acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

A method of finding a biocatalyst having ammonia lyase activity, in particular a lysine ammonia lyase, comprising - providing a library comprising a plurality of cells in one or more cell cultures, which cells are candidates for having an ammonia lyase activity, which one or more cultures comprise a culture medium containing at least one nitrogen source selected from the group of amines, including amino acids, and structural analogues thereof, as sole nitrogen source or sources for the cells; - selecting at least one candidate which grows in said culture medium; and - screening the selected candidate or candidates which grow in said culture for the ammonia lyase activity.
PCT/NL2010/050876 2009-12-22 2010-12-22 Method of finding a biocatalyst having ammonia lyase activity WO2011078667A2 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP09180416 2009-12-22
EP09180416.1 2009-12-22

Publications (2)

Publication Number Publication Date
WO2011078667A2 WO2011078667A2 (en) 2011-06-30
WO2011078667A3 true WO2011078667A3 (en) 2011-08-18

Family

ID=42102600

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/NL2010/050876 WO2011078667A2 (en) 2009-12-22 2010-12-22 Method of finding a biocatalyst having ammonia lyase activity

Country Status (1)

Country Link
WO (1) WO2011078667A2 (en)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2870123A4 (en) * 2012-07-06 2016-02-17 Univ Monash Processes for producing amine compounds
EP2971023A4 (en) 2013-03-15 2017-03-15 Kiverdi, Inc. Methods of using natural and engineered organisms to produce small molecules for industrial application

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050065176A1 (en) * 2003-09-22 2005-03-24 Field Mark John Combinations
EP2123768A1 (en) * 2008-05-20 2009-11-25 DSM IP Assets B.V. Preparation of (Z)-6,7-dihydro-1H-azepin-2(5H)-one
WO2009142489A2 (en) * 2008-05-20 2009-11-26 Dsm Ip Assets B.V. Preparation of alpha-amino-epsilon-caprolactam via lysine cyclisation

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR100516986B1 (en) 1997-02-19 2005-09-26 코닌클리즈케 디에스엠 엔.브이. Process for the preparation of caprolactam in the absence of catalysts by contacting 6-aminocaproic acid derivatives with superheated steam
WO2005068643A2 (en) 2004-01-19 2005-07-28 Dsm Ip Assets B.V. Biochemical synthesis of 6-amino caproic acid
US7537923B2 (en) 2007-08-17 2009-05-26 Biomarin Pharmaceutical Inc. Compositions of prokaryotic phenylalanine ammonia-lyase and methods of treating cancer using compositions thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20050065176A1 (en) * 2003-09-22 2005-03-24 Field Mark John Combinations
EP2123768A1 (en) * 2008-05-20 2009-11-25 DSM IP Assets B.V. Preparation of (Z)-6,7-dihydro-1H-azepin-2(5H)-one
WO2009142489A2 (en) * 2008-05-20 2009-11-26 Dsm Ip Assets B.V. Preparation of alpha-amino-epsilon-caprolactam via lysine cyclisation

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
KREIMEYER ANNETT ET AL: "Identification of the last unknown genes in the fermentation pathway of lysine.", THE JOURNAL OF BIOLOGICAL CHEMISTRY 9 MAR 2007 LNKD- PUBMED:17166837, vol. 282, no. 10, 9 March 2007 (2007-03-09), pages 7191 - 7197, XP002579490, ISSN: 0021-9258 *
PETER S J CHEETHAM: "Chapter 2. Case studies in applied biocatalysis-from ideas to products", APPLIED CATALYSIS,, 1 January 1994 (1994-01-01), pages 47 - 108, XP009132517 *
THIRUVENGADAM T K ET AL: "BIOSYNTHESIS OF STREPTOTHRICIN F 5. FORMATION OF BETA LYSINE BY STREPTOMYCES L-1689-23", JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, vol. 105, no. 16, 1983, pages 5470 - 5476, XP002635052, ISSN: 0002-7863 *

Also Published As

Publication number Publication date
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