CA3212565A1 - Leader peptides for expressing a protein of interest in an eukaryotic host cell - Google Patents

Leader peptides for expressing a protein of interest in an eukaryotic host cell

Info

Publication number
CA3212565A1
CA3212565A1 CA3212565A CA3212565A CA3212565A1 CA 3212565 A1 CA3212565 A1 CA 3212565A1 CA 3212565 A CA3212565 A CA 3212565A CA 3212565 A CA3212565 A CA 3212565A CA 3212565 A1 CA3212565 A1 CA 3212565A1
Authority
CA
Canada
Prior art keywords
poi
seq
leader
amino acid
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3212565A
Other languages
English (en)
French (fr)
Inventor
Brigitte Gasser
Diethard Mattanovich
Silvia HEISS
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lonza AG
Original Assignee
Lonza AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lonza AG filed Critical Lonza AG
Publication of CA3212565A1 publication Critical patent/CA3212565A1/en
Pending legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/76—Albumins
    • C07K14/765—Serum albumin, e.g. HSA
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6478—Aspartic endopeptidases (3.4.23)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Immunology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CA3212565A 2012-10-29 2013-10-29 Leader peptides for expressing a protein of interest in an eukaryotic host cell Pending CA3212565A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12190361 2012-10-29
EP12190361.1 2012-10-29
CA2886460A CA2886460C (en) 2012-10-29 2013-10-29 Leader peptides for expressing a protein of interest in an eukaryotic host cell

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CA2886460A Division CA2886460C (en) 2012-10-29 2013-10-29 Leader peptides for expressing a protein of interest in an eukaryotic host cell

Publications (1)

Publication Number Publication Date
CA3212565A1 true CA3212565A1 (en) 2014-05-08

Family

ID=47115533

Family Applications (2)

Application Number Title Priority Date Filing Date
CA3212565A Pending CA3212565A1 (en) 2012-10-29 2013-10-29 Leader peptides for expressing a protein of interest in an eukaryotic host cell
CA2886460A Active CA2886460C (en) 2012-10-29 2013-10-29 Leader peptides for expressing a protein of interest in an eukaryotic host cell

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2886460A Active CA2886460C (en) 2012-10-29 2013-10-29 Leader peptides for expressing a protein of interest in an eukaryotic host cell

Country Status (16)

Country Link
US (2) US10160988B2 (enExample)
EP (2) EP3130598B1 (enExample)
JP (2) JP6556625B2 (enExample)
KR (1) KR102079293B1 (enExample)
CN (1) CN104837857B (enExample)
AU (1) AU2013341049B2 (enExample)
BR (1) BR112015008289A2 (enExample)
CA (2) CA3212565A1 (enExample)
DK (1) DK2912053T3 (enExample)
ES (2) ES2789299T3 (enExample)
HU (1) HUE033282T2 (enExample)
IL (2) IL238420B (enExample)
IN (1) IN2015DN02771A (enExample)
PL (1) PL2912053T3 (enExample)
SG (1) SG11201502745XA (enExample)
WO (1) WO2014067926A1 (enExample)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102079293B1 (ko) * 2012-10-29 2020-02-19 론자 리미티드 발현 서열
CN104312933B (zh) * 2014-10-17 2017-03-29 江南大学 一种优化信号肽提高胰蛋白酶胞外分泌表达的方法
WO2017021541A1 (en) * 2015-08-05 2017-02-09 Lonza Ltd Promoter variants
EP3423588A1 (en) 2016-03-02 2019-01-09 Lonza Ltd Improved fermentation process
KR102486713B1 (ko) * 2017-03-10 2023-01-10 볼트 쓰레즈, 인크. 재조합 단백질을 고분비 수율로 생산하기 위한 조성물 및 방법
CA3072134A1 (en) 2017-05-31 2018-12-06 Universitat Fur Bodenkultur Wien Yeast expressing a synthetic calvin cycle
JP7717459B2 (ja) 2018-02-12 2025-08-04 ロンザ リミテッド 目的タンパク質を産生するための宿主細胞
US20210388037A1 (en) * 2018-11-19 2021-12-16 Basf Se Leader sequence for yeast
JP7061234B2 (ja) 2019-01-11 2022-04-27 ロンザ リミテッド 組換え宿主細胞内での炭素源調節タンパク質産生
JP7570346B2 (ja) 2019-04-01 2024-10-21 ウニヴェルズィテート・フューア・ボーデンクルトゥーア・ウィーン Ｍｕｔ－メチロトローフ酵母
WO2020200414A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Protein production in mut-methylotrophic yeast
WO2020200415A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Mut- methylotrophic yeast
CN110760003A (zh) * 2019-09-10 2020-02-07 广东药科大学 一种抗her3单链抗体的制备方法
ES3034258T3 (en) 2020-04-01 2025-08-14 Lonza Ag Helper factors for expressing proteins in yeast
CN116490517A (zh) 2020-09-30 2023-07-25 龙沙有限公司 过表达翻译因子的宿主细胞
WO2022144374A2 (en) 2020-12-29 2022-07-07 Bisy Gmbh Novel yeast strains
CN117794941A (zh) * 2021-07-30 2024-03-29 赫莱纳公司 用于蛋白质合成和分泌的方法和组合物
EP4424698A3 (en) * 2021-07-30 2024-11-27 Helaina, Inc. Methods and compositions for protein synthesis and secretion
WO2024133538A1 (en) 2022-12-20 2024-06-27 Lonza Ltd Host cells with pat1 knockout for increased specific protein productivity
WO2024141641A2 (en) * 2022-12-30 2024-07-04 Biotalys NV Secretion signals
WO2026009000A1 (en) 2024-07-04 2026-01-08 Solasta Bio Limited Methods for production of insecticidal neuropeptides

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0118551A1 (en) 1982-09-15 1984-09-19 Collaborative Research Inc. Production of interferon in yeast by use of invertase promoter
US5010182A (en) 1987-07-28 1991-04-23 Chiron Corporation DNA constructs containing a Kluyveromyces alpha factor leader sequence for directing secretion of heterologous polypeptides
CA1340772C (en) 1987-12-30 1999-09-28 Patricia Tekamp-Olson Expression and secretion of heterologous protiens in yeast employing truncated alpha-factor leader sequences
JPH05184352A (ja) 1990-01-16 1993-07-27 Centro De Ing Genetica Y Biotecnol ピヒア パストリス（Ｐｉｃｈｉａ ｐａｓｔｏｒｉｓ）酵母中での異種遺伝子の発現方法、発現ベクターおよび形質転換微生物
US5268273A (en) 1990-12-14 1993-12-07 Phillips Petroleum Company Pichia pastoris acid phosphatase gene, gene regions, signal sequence and expression vectors comprising same
DK82893D0 (da) 1993-07-08 1993-07-08 Novo Nordisk As Peptid
CN101679992A (zh) 2007-04-20 2010-03-24 波利门科学生物免疫研究有限公司 酵母菌表达系统
US7741075B2 (en) 2008-06-06 2010-06-22 Board Of Regents Of The University Of Nebraska Pichia pastoris PIR1 secretion signal peptide for recombinant protein expression and Pichia pastoris PIR1 and PIR2 anchor domain peptides for recombinant surface display
US8440456B2 (en) 2009-05-22 2013-05-14 Vib, Vzw Nucleic acids of Pichia pastoris and use thereof for recombinant production of proteins
EP2258855A1 (en) * 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
DE102009055015B4 (de) 2009-12-18 2013-12-24 Technische Universität Dresden Hefe-Sekretionssystem auf Basis einer Signalsequenz aus der Hydrophobin 1-Gensequenz von Trichoderma reesei
WO2012152823A1 (en) * 2011-05-09 2012-11-15 Ablynx Nv Method for the production of immunoglobulin single variable domains
KR102079293B1 (ko) * 2012-10-29 2020-02-19 론자 리미티드 발현 서열

Also Published As

Publication number Publication date
PL2912053T3 (pl) 2017-05-31
DK2912053T3 (en) 2017-01-30
IL266700A (en) 2019-07-31
CA2886460C (en) 2023-11-14
JP2015533286A (ja) 2015-11-24
BR112015008289A2 (pt) 2017-11-28
HK1208037A1 (en) 2016-02-19
KR102079293B1 (ko) 2020-02-19
JP2019141063A (ja) 2019-08-29
CA2886460A1 (en) 2014-05-08
ES2610990T3 (es) 2017-05-04
EP2912053B1 (en) 2016-11-09
KR20150076160A (ko) 2015-07-06
ES2789299T3 (es) 2020-10-26
HUE033282T2 (en) 2017-11-28
EP3130598A2 (en) 2017-02-15
US20150232904A1 (en) 2015-08-20
AU2013341049A1 (en) 2015-04-02
WO2014067926A1 (en) 2014-05-08
EP3130598B1 (en) 2020-02-12
IN2015DN02771A (enExample) 2015-09-04
EP3130598A3 (en) 2017-05-03
IL266700B (en) 2021-08-31
EP2912053A1 (en) 2015-09-02
CN104837857B (zh) 2018-11-06
US10160988B2 (en) 2018-12-25
US20180135092A1 (en) 2018-05-17
IL238420A0 (en) 2015-06-30
JP6556625B2 (ja) 2019-08-07
US11359223B2 (en) 2022-06-14
IL238420B (en) 2019-05-30
CN104837857A (zh) 2015-08-12
SG11201502745XA (en) 2015-05-28
AU2013341049B2 (en) 2017-07-20

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