CA3206960A1 - Synthetic polynucleotides and methods for selectively amplifying alleles - Google Patents

Synthetic polynucleotides and methods for selectively amplifying alleles Download PDF

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Publication number
CA3206960A1
CA3206960A1 CA3206960A CA3206960A CA3206960A1 CA 3206960 A1 CA3206960 A1 CA 3206960A1 CA 3206960 A CA3206960 A CA 3206960A CA 3206960 A CA3206960 A CA 3206960A CA 3206960 A1 CA3206960 A1 CA 3206960A1
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CA
Canada
Prior art keywords
selector
polynucleotide
optionally
residue
dna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CA3206960A
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English (en)
French (fr)
Inventor
Charles Rodi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Rhodx Inc
Original Assignee
Rhodx Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Rhodx Inc filed Critical Rhodx Inc
Publication of CA3206960A1 publication Critical patent/CA3206960A1/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/26Preparation of nitrogen-containing carbohydrates
    • C12P19/28N-glycosides
    • C12P19/30Nucleotides
    • C12P19/34Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6853Nucleic acid amplification reactions using modified primers or templates
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Pathology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oncology (AREA)
  • Hospice & Palliative Care (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CA3206960A 2021-02-02 2022-02-02 Synthetic polynucleotides and methods for selectively amplifying alleles Pending CA3206960A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163144723P 2021-02-02 2021-02-02
US63/144,723 2021-02-02
PCT/US2022/014908 WO2022169846A1 (en) 2021-02-02 2022-02-02 Synthetic polynucleotides and methods for selectively amplifying alleles

Publications (1)

Publication Number Publication Date
CA3206960A1 true CA3206960A1 (en) 2022-08-11

Family

ID=82741675

Family Applications (1)

Application Number Title Priority Date Filing Date
CA3206960A Pending CA3206960A1 (en) 2021-02-02 2022-02-02 Synthetic polynucleotides and methods for selectively amplifying alleles

Country Status (8)

Country Link
US (1) US20240301481A1 (https=)
EP (1) EP4288557A4 (https=)
JP (1) JP2024505119A (https=)
KR (1) KR20230141826A (https=)
CN (1) CN116888271A (https=)
AU (1) AU2022216246A1 (https=)
CA (1) CA3206960A1 (https=)
WO (1) WO2022169846A1 (https=)

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2222510A1 (en) * 1995-06-07 1996-12-19 Gen-Probe Incorporated Template and primer based synthesis of enzymatically cleavable oligonucleotides
US5916777A (en) * 1995-06-07 1999-06-29 Gen-Probe Incorporated Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers
CN1254548C (zh) * 2000-08-23 2006-05-03 宝生物工程株式会社 核酸扩增方法
WO2002064833A1 (en) * 2001-02-15 2002-08-22 Takara Bio Inc. Method of detecting nucleotide polymorphism
US10227641B2 (en) * 2008-04-30 2019-03-12 Integrated Dna Technologies, Inc. RNase H-based assays utilizing modified RNA monomers
ES2384681T3 (es) * 2008-08-12 2012-07-10 F. Hoffmann-La Roche Ag Extensión de cebadores correctora
WO2011085075A2 (en) * 2010-01-07 2011-07-14 Gen9, Inc. Assembly of high fidelity polynucleotides
CA2863808C (en) * 2012-02-20 2020-07-21 Speedx Pty Ltd Methods and oligonucleotides for amplifying and/or detecting nucleic acids
UA116205C2 (uk) * 2012-04-09 2018-02-26 Енвіролоджикс Інк. Спосіб кількісного визначення послідовності нуклеїнової кислоти в зразку
WO2017142989A1 (en) * 2016-02-17 2017-08-24 Admera Health LLC Nucleic acid preparation and analysis

Also Published As

Publication number Publication date
EP4288557A4 (en) 2024-12-25
EP4288557A1 (en) 2023-12-13
WO2022169846A1 (en) 2022-08-11
AU2022216246A1 (en) 2023-09-14
KR20230141826A (ko) 2023-10-10
JP2024505119A (ja) 2024-02-02
US20240301481A1 (en) 2024-09-12
CN116888271A (zh) 2023-10-13

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