CN116888271A - 用于选择性扩增等位基因的合成多核苷酸和方法 - Google Patents
用于选择性扩增等位基因的合成多核苷酸和方法 Download PDFInfo
- Publication number
- CN116888271A CN116888271A CN202280012877.5A CN202280012877A CN116888271A CN 116888271 A CN116888271 A CN 116888271A CN 202280012877 A CN202280012877 A CN 202280012877A CN 116888271 A CN116888271 A CN 116888271A
- Authority
- CN
- China
- Prior art keywords
- selector
- polynucleotide
- optionally
- residue
- dna
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Analytical Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- General Chemical & Material Sciences (AREA)
- Oncology (AREA)
- Hospice & Palliative Care (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163144723P | 2021-02-02 | 2021-02-02 | |
| US63/144,723 | 2021-02-02 | ||
| PCT/US2022/014908 WO2022169846A1 (en) | 2021-02-02 | 2022-02-02 | Synthetic polynucleotides and methods for selectively amplifying alleles |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116888271A true CN116888271A (zh) | 2023-10-13 |
Family
ID=82741675
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202280012877.5A Pending CN116888271A (zh) | 2021-02-02 | 2022-02-02 | 用于选择性扩增等位基因的合成多核苷酸和方法 |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20240301481A1 (https=) |
| EP (1) | EP4288557A4 (https=) |
| JP (1) | JP2024505119A (https=) |
| KR (1) | KR20230141826A (https=) |
| CN (1) | CN116888271A (https=) |
| AU (1) | AU2022216246A1 (https=) |
| CA (1) | CA3206960A1 (https=) |
| WO (1) | WO2022169846A1 (https=) |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2222510A1 (en) * | 1995-06-07 | 1996-12-19 | Gen-Probe Incorporated | Template and primer based synthesis of enzymatically cleavable oligonucleotides |
| US5916777A (en) * | 1995-06-07 | 1999-06-29 | Gen-Probe Incorporated | Enzymatic synthesis of oligonucleotides using 3'-ribonucleotide primers |
| CN1254548C (zh) * | 2000-08-23 | 2006-05-03 | 宝生物工程株式会社 | 核酸扩增方法 |
| WO2002064833A1 (en) * | 2001-02-15 | 2002-08-22 | Takara Bio Inc. | Method of detecting nucleotide polymorphism |
| US10227641B2 (en) * | 2008-04-30 | 2019-03-12 | Integrated Dna Technologies, Inc. | RNase H-based assays utilizing modified RNA monomers |
| ES2384681T3 (es) * | 2008-08-12 | 2012-07-10 | F. Hoffmann-La Roche Ag | Extensión de cebadores correctora |
| WO2011085075A2 (en) * | 2010-01-07 | 2011-07-14 | Gen9, Inc. | Assembly of high fidelity polynucleotides |
| CA2863808C (en) * | 2012-02-20 | 2020-07-21 | Speedx Pty Ltd | Methods and oligonucleotides for amplifying and/or detecting nucleic acids |
| UA116205C2 (uk) * | 2012-04-09 | 2018-02-26 | Енвіролоджикс Інк. | Спосіб кількісного визначення послідовності нуклеїнової кислоти в зразку |
| WO2017142989A1 (en) * | 2016-02-17 | 2017-08-24 | Admera Health LLC | Nucleic acid preparation and analysis |
-
2022
- 2022-02-02 AU AU2022216246A patent/AU2022216246A1/en active Pending
- 2022-02-02 EP EP22750306.7A patent/EP4288557A4/en active Pending
- 2022-02-02 KR KR1020237029568A patent/KR20230141826A/ko active Pending
- 2022-02-02 WO PCT/US2022/014908 patent/WO2022169846A1/en not_active Ceased
- 2022-02-02 US US18/274,453 patent/US20240301481A1/en active Pending
- 2022-02-02 JP JP2023571247A patent/JP2024505119A/ja active Pending
- 2022-02-02 CA CA3206960A patent/CA3206960A1/en active Pending
- 2022-02-02 CN CN202280012877.5A patent/CN116888271A/zh active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP4288557A4 (en) | 2024-12-25 |
| EP4288557A1 (en) | 2023-12-13 |
| CA3206960A1 (en) | 2022-08-11 |
| WO2022169846A1 (en) | 2022-08-11 |
| AU2022216246A1 (en) | 2023-09-14 |
| KR20230141826A (ko) | 2023-10-10 |
| JP2024505119A (ja) | 2024-02-02 |
| US20240301481A1 (en) | 2024-09-12 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |