CA3019589A1 - Accurate molecular barcoding - Google Patents
Accurate molecular barcoding Download PDFInfo
- Publication number
- CA3019589A1 CA3019589A1 CA3019589A CA3019589A CA3019589A1 CA 3019589 A1 CA3019589 A1 CA 3019589A1 CA 3019589 A CA3019589 A CA 3019589A CA 3019589 A CA3019589 A CA 3019589A CA 3019589 A1 CA3019589 A1 CA 3019589A1
- Authority
- CA
- Canada
- Prior art keywords
- oligonucleotide species
- barcode
- unique oligonucleotide
- unique
- molecule
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
- C12Q1/6837—Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201662330500P | 2016-05-02 | 2016-05-02 | |
| US62/330,500 | 2016-05-02 | ||
| PCT/US2017/030097 WO2017192387A1 (en) | 2016-05-02 | 2017-04-28 | Accurate molecular barcoding |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA3019589A1 true CA3019589A1 (en) | 2017-11-09 |
Family
ID=59285319
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA3019589A Pending CA3019589A1 (en) | 2016-05-02 | 2017-04-28 | Accurate molecular barcoding |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US10822643B2 (https=) |
| EP (2) | EP3452614B1 (https=) |
| JP (2) | JP7129343B2 (https=) |
| CN (1) | CN109072288A (https=) |
| AU (2) | AU2017261189B2 (https=) |
| CA (1) | CA3019589A1 (https=) |
| ES (1) | ES2956757T3 (https=) |
| WO (1) | WO2017192387A1 (https=) |
Families Citing this family (45)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| CA2865575C (en) | 2012-02-27 | 2024-01-16 | Cellular Research, Inc. | Compositions and kits for molecular counting |
| KR102758333B1 (ko) | 2013-08-28 | 2025-01-23 | 벡톤 디킨슨 앤드 컴퍼니 | 대량의 동시 단일 세포 분석 |
| US9582877B2 (en) | 2013-10-07 | 2017-02-28 | Cellular Research, Inc. | Methods and systems for digitally counting features on arrays |
| EP3259371B1 (en) | 2015-02-19 | 2020-09-02 | Becton, Dickinson and Company | High-throughput single-cell analysis combining proteomic and genomic information |
| WO2016138496A1 (en) | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
| WO2016160844A2 (en) | 2015-03-30 | 2016-10-06 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
| EP4582556A3 (en) | 2015-04-23 | 2025-10-08 | Becton, Dickinson and Company | Methods and compositions for whole transcriptome amplification |
| WO2016196229A1 (en) | 2015-06-01 | 2016-12-08 | Cellular Research, Inc. | Methods for rna quantification |
| JP6940484B2 (ja) | 2015-09-11 | 2021-09-29 | セルラー リサーチ， インコーポレイテッド | ライブラリー正規化のための方法および組成物 |
| JP7129343B2 (ja) | 2016-05-02 | 2022-09-01 | ベクトン・ディキンソン・アンド・カンパニー | 正確な分子バーコーディング |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| ES2979395T3 (es) | 2016-05-26 | 2024-09-25 | Becton Dickinson Co | Métodos de ajuste del recuento de etiquetas moleculares |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| CN109791157B (zh) | 2016-09-26 | 2022-06-07 | 贝克顿迪金森公司 | 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达 |
| ES2961580T3 (es) | 2017-01-13 | 2024-03-12 | Cellular Res Inc | Revestimiento hidrófilo de canales de fluidos |
| CN110382708A (zh) | 2017-02-01 | 2019-10-25 | 赛卢拉研究公司 | 使用阻断性寡核苷酸进行选择性扩增 |
| CN110719959B (zh) | 2017-06-05 | 2021-08-06 | 贝克顿迪金森公司 | 针对单细胞的样品索引 |
| EP3728636B1 (en) | 2017-12-19 | 2024-09-11 | Becton, Dickinson and Company | Particles associated with oligonucleotides |
| JP7358388B2 (ja) | 2018-05-03 | 2023-10-10 | ベクトン・ディキンソン・アンド・カンパニー | 反対側の転写物末端における分子バーコーディング |
| CN112272710A (zh) | 2018-05-03 | 2021-01-26 | 贝克顿迪金森公司 | 高通量多组学样品分析 |
| ES2992135T3 (es) | 2018-10-01 | 2024-12-09 | Becton Dickinson Co | Determinar secuencias de transcripción 5 |
| US11932849B2 (en) | 2018-11-08 | 2024-03-19 | Becton, Dickinson And Company | Whole transcriptome analysis of single cells using random priming |
| US11492660B2 (en) | 2018-12-13 | 2022-11-08 | Becton, Dickinson And Company | Selective extension in single cell whole transcriptome analysis |
| US11371076B2 (en) | 2019-01-16 | 2022-06-28 | Becton, Dickinson And Company | Polymerase chain reaction normalization through primer titration |
| ES2945227T3 (es) | 2019-01-23 | 2023-06-29 | Becton Dickinson Co | Oligonucleótidos asociados con anticuerpos |
| WO2020167920A1 (en) | 2019-02-14 | 2020-08-20 | Cellular Research, Inc. | Hybrid targeted and whole transcriptome amplification |
| US11965208B2 (en) | 2019-04-19 | 2024-04-23 | Becton, Dickinson And Company | Methods of associating phenotypical data and single cell sequencing data |
| CN114423873B (zh) * | 2019-07-10 | 2025-06-13 | 阿尔缇玛基因组学公司 | Rna测序方法 |
| CN120099137A (zh) | 2019-07-22 | 2025-06-06 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| US10927409B1 (en) | 2019-10-14 | 2021-02-23 | Pioneer Hi-Bred International, Inc. | Detection of sequences uniquely associated with a dna target region |
| JP7522189B2 (ja) | 2019-11-08 | 2024-07-24 | ベクトン・ディキンソン・アンド・カンパニー | 免疫レパートリーシーケンシングのための完全長ｖ（ｄ）ｊ情報を得るためのランダムプライミングの使用 |
| US11649497B2 (en) | 2020-01-13 | 2023-05-16 | Becton, Dickinson And Company | Methods and compositions for quantitation of proteins and RNA |
| WO2021146219A1 (en) | 2020-01-13 | 2021-07-22 | Becton, Dickinson And Company | Cell capture using du-containing oligonucleotides |
| WO2021155057A1 (en) | 2020-01-29 | 2021-08-05 | Becton, Dickinson And Company | Barcoded wells for spatial mapping of single cells through sequencing |
| WO2021173719A1 (en) | 2020-02-25 | 2021-09-02 | Becton, Dickinson And Company | Bi-specific probes to enable the use of single-cell samples as single color compensation control |
| KR102482668B1 (ko) * | 2020-03-10 | 2022-12-29 | 사회복지법인 삼성생명공익재단 | 고유 분자 식별자의 표지 정확도를 증진하는 방법 |
| WO2021195277A1 (en) * | 2020-03-24 | 2021-09-30 | President And Fellows Of Harvard College | Multiplexed methods for detecting target rnas |
| US11661625B2 (en) | 2020-05-14 | 2023-05-30 | Becton, Dickinson And Company | Primers for immune repertoire profiling |
| US12157913B2 (en) | 2020-06-02 | 2024-12-03 | Becton, Dickinson And Company | Oligonucleotides and beads for 5 prime gene expression assay |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| US12391940B2 (en) | 2020-07-31 | 2025-08-19 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| EP4247967A1 (en) | 2020-11-20 | 2023-09-27 | Becton, Dickinson and Company | Profiling of highly expressed and lowly expressed proteins |
| CN116685850A (zh) | 2020-12-15 | 2023-09-01 | 贝克顿迪金森公司 | 单细胞分泌组分析 |
Family Cites Families (324)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4510244A (en) | 1980-04-17 | 1985-04-09 | The Board Of Trustees Of The Leland Stanford Jr. University | Cell labeling with antigen-coupled microspheres |
| US4725536A (en) | 1985-09-19 | 1988-02-16 | Genetics Institute, Inc. | Reagent polynucleotide complex with multiple target binding regions, and kit and methods |
| US6150517A (en) | 1986-11-24 | 2000-11-21 | Gen-Probe | Methods for making oligonucleotide probes for the detection and/or quantitation of non-viral organisms |
| WO1989001050A1 (en) | 1987-07-31 | 1989-02-09 | The Board Of Trustees Of The Leland Stanford Junior University | Selective amplification of target polynucleotide sequences |
| US5149625A (en) | 1987-08-11 | 1992-09-22 | President And Fellows Of Harvard College | Multiplex analysis of DNA |
| US5124246A (en) | 1987-10-15 | 1992-06-23 | Chiron Corporation | Nucleic acid multimers and amplified nucleic acid hybridization assays using same |
| US5656731A (en) | 1987-10-15 | 1997-08-12 | Chiron Corporation | Nucleic acid-amplified immunoassay probes |
| EP0426756A4 (en) | 1988-07-26 | 1991-11-21 | Genelabs Incorporated | Rna and dna amplification techniques |
| US5800992A (en) | 1989-06-07 | 1998-09-01 | Fodor; Stephen P.A. | Method of detecting nucleic acids |
| US5871928A (en) | 1989-06-07 | 1999-02-16 | Fodor; Stephen P. A. | Methods for nucleic acid analysis |
| US5424186A (en) | 1989-06-07 | 1995-06-13 | Affymax Technologies N.V. | Very large scale immobilized polymer synthesis |
| US5925525A (en) | 1989-06-07 | 1999-07-20 | Affymetrix, Inc. | Method of identifying nucleotide differences |
| US5143854A (en) | 1989-06-07 | 1992-09-01 | Affymax Technologies N.V. | Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof |
| US5744101A (en) | 1989-06-07 | 1998-04-28 | Affymax Technologies N.V. | Photolabile nucleoside protecting groups |
| US6040138A (en) | 1995-09-15 | 2000-03-21 | Affymetrix, Inc. | Expression monitoring by hybridization to high density oligonucleotide arrays |
| US6309822B1 (en) | 1989-06-07 | 2001-10-30 | Affymetrix, Inc. | Method for comparing copy number of nucleic acid sequences |
| US6551784B2 (en) | 1989-06-07 | 2003-04-22 | Affymetrix Inc | Method of comparing nucleic acid sequences |
| US5200314A (en) | 1990-03-23 | 1993-04-06 | Chiron Corporation | Polynucleotide capture assay employing in vitro amplification |
| EP0834575B1 (en) | 1990-12-06 | 2001-11-28 | Affymetrix, Inc. (a Delaware Corporation) | Identification of nucleic acids in samples |
| US5981179A (en) | 1991-11-14 | 1999-11-09 | Digene Diagnostics, Inc. | Continuous amplification reaction |
| US5424413A (en) | 1992-01-22 | 1995-06-13 | Gen-Probe Incorporated | Branched nucleic acid probes |
| US5573905A (en) | 1992-03-30 | 1996-11-12 | The Scripps Research Institute | Encoded combinatorial chemical libraries |
| US5981176A (en) | 1992-06-17 | 1999-11-09 | City Of Hope | Method of detecting and discriminating between nucleic acid sequences |
| DE69431719T2 (de) | 1993-06-25 | 2003-09-18 | Affymetrix, Inc. (N.D.Ges.D.Staates Delaware) | Hybridisierung und sequenzierung von nukleinsäuren |
| US6087186A (en) | 1993-07-16 | 2000-07-11 | Irori | Methods and apparatus for synthesizing labeled combinatorial chemistry libraries |
| US5500356A (en) | 1993-08-10 | 1996-03-19 | Life Technologies, Inc. | Method of nucleic acid sequence selection |
| US6309823B1 (en) | 1993-10-26 | 2001-10-30 | Affymetrix, Inc. | Arrays of nucleic acid probes for analyzing biotransformation genes and methods of using the same |
| US5681697A (en) | 1993-12-08 | 1997-10-28 | Chiron Corporation | Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor |
| US5714330A (en) | 1994-04-04 | 1998-02-03 | Lynx Therapeutics, Inc. | DNA sequencing by stepwise ligation and cleavage |
| US5604097A (en) | 1994-10-13 | 1997-02-18 | Spectragen, Inc. | Methods for sorting polynucleotides using oligonucleotide tags |
| US6013445A (en) | 1996-06-06 | 2000-01-11 | Lynx Therapeutics, Inc. | Massively parallel signature sequencing by ligation of encoded adaptors |
| US5695934A (en) | 1994-10-13 | 1997-12-09 | Lynx Therapeutics, Inc. | Massively parallel sequencing of sorted polynucleotides |
| US5846719A (en) | 1994-10-13 | 1998-12-08 | Lynx Therapeutics, Inc. | Oligonucleotide tags for sorting and identification |
| US6600996B2 (en) | 1994-10-21 | 2003-07-29 | Affymetrix, Inc. | Computer-aided techniques for analyzing biological sequences |
| DE69535240T2 (de) | 1994-10-28 | 2007-06-06 | Gen-Probe Inc., San Diego | Zusammensetzungen und Verfahren für die gleichzeitige Detektion und Quantifizierung von einer Mehrheit spezifischer Nuklein Säure Sequenzen |
| CA2216645A1 (en) | 1995-04-25 | 1996-11-21 | Irori | Remotely programmable matrices with memories and uses thereof |
| US5648245A (en) | 1995-05-09 | 1997-07-15 | Carnegie Institution Of Washington | Method for constructing an oligonucleotide concatamer library by rolling circle replication |
| US5968740A (en) | 1995-07-24 | 1999-10-19 | Affymetrix, Inc. | Method of Identifying a Base in a Nucleic Acid |
| US5763175A (en) | 1995-11-17 | 1998-06-09 | Lynx Therapeutics, Inc. | Simultaneous sequencing of tagged polynucleotides |
| US5854033A (en) | 1995-11-21 | 1998-12-29 | Yale University | Rolling circle replication reporter systems |
| US6613544B1 (en) | 1995-12-22 | 2003-09-02 | Amgen Inc. | Osteoprotegerin |
| US5962271A (en) | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
| US6458530B1 (en) | 1996-04-04 | 2002-10-01 | Affymetrix Inc. | Selecting tag nucleic acids |
| EP2369007B1 (en) | 1996-05-29 | 2015-07-29 | Cornell Research Foundation, Inc. | Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions |
| JPH1021373A (ja) | 1996-07-05 | 1998-01-23 | Fuji Photo Film Co Ltd | 画像解析装置 |
| US5935793A (en) | 1996-09-27 | 1999-08-10 | The Chinese University Of Hong Kong | Parallel polynucleotide sequencing method using tagged primers |
| US6124092A (en) | 1996-10-04 | 2000-09-26 | The Perkin-Elmer Corporation | Multiplex polynucleotide capture methods and compositions |
| US6117631A (en) | 1996-10-29 | 2000-09-12 | Polyprobe, Inc. | Detection of antigens via oligonucleotide antibody conjugates |
| US6046005A (en) | 1997-01-15 | 2000-04-04 | Incyte Pharmaceuticals, Inc. | Nucleic acid sequencing with solid phase capturable terminators comprising a cleavable linking group |
| JP4294740B2 (ja) | 1997-05-23 | 2009-07-15 | ソレクサ・インコーポレイテッド | 分析物の系列的プロセシングのためのシステムおよび装置 |
| US6399334B1 (en) | 1997-09-24 | 2002-06-04 | Invitrogen Corporation | Normalized nucleic acid libraries and methods of production thereof |
| AU1603199A (en) | 1997-12-03 | 1999-06-16 | Curagen Corporation | Methods and devices for measuring differential gene expression |
| US6265163B1 (en) | 1998-01-09 | 2001-07-24 | Lynx Therapeutics, Inc. | Solid phase selection of differentially expressed genes |
| US6787308B2 (en) | 1998-07-30 | 2004-09-07 | Solexa Ltd. | Arrayed biomolecules and their use in sequencing |
| WO2000014282A1 (en) | 1998-09-04 | 2000-03-16 | Lynx Therapeutics, Inc. | Method of screening for genetic polymorphism |
| US6480791B1 (en) | 1998-10-28 | 2002-11-12 | Michael P. Strathmann | Parallel methods for genomic analysis |
| US6197554B1 (en) | 1998-11-20 | 2001-03-06 | Shi-Lung Lin | Method for generating full-length cDNA library from single cells |
| US6436675B1 (en) | 1999-09-28 | 2002-08-20 | Maxygen, Inc. | Use of codon-varied oligonucleotide synthesis for synthetic shuffling |
| PT1033364E (pt) * | 1999-03-01 | 2005-07-29 | Pfizer Prod Inc | Ciano com acidos oxamicos e derivados como ligandos de receptores de tiroide |
| US6629040B1 (en) | 1999-03-19 | 2003-09-30 | University Of Washington | Isotope distribution encoded tags for protein identification |
| JP2002539849A (ja) | 1999-03-26 | 2002-11-26 | ホワイトヘッド インスチチュート フォアー バイオメディカル リサーチ | ユニバーサルアレイ |
| US6355431B1 (en) | 1999-04-20 | 2002-03-12 | Illumina, Inc. | Detection of nucleic acid amplification reactions using bead arrays |
| EP1046717B1 (en) | 1999-04-20 | 2010-10-06 | National Institute of Advanced Industrial Science and Technology | Method and probes for determining a concentration of target nucleic acid molecules and method for analyzing data obtained by the method |
| US6372813B1 (en) | 1999-06-25 | 2002-04-16 | Motorola | Methods and compositions for attachment of biomolecules to solid supports, hydrogels, and hydrogel arrays |
| US6326148B1 (en) | 1999-07-12 | 2001-12-04 | The Regents Of The University Of California | Detection of copy number changes in colon cancer |
| US6440706B1 (en) | 1999-08-02 | 2002-08-27 | Johns Hopkins University | Digital amplification |
| SE9903988D0 (sv) | 1999-11-03 | 1999-11-03 | Amersham Pharm Biotech Ab | Method of analysis |
| US6489114B2 (en) | 1999-12-17 | 2002-12-03 | Bio Merieux | Process for labeling a ribonucleic acid, and labeled RNA fragments which are obtained thereby |
| US6235483B1 (en) | 2000-01-31 | 2001-05-22 | Agilent Technologies, Inc. | Methods and kits for indirect labeling of nucleic acids |
| DK1259643T3 (da) | 2000-02-07 | 2009-02-23 | Illumina Inc | Fremgangsmåde til detektion af nukleinsyre ved anvendelse af universel priming |
| GB2364054B (en) | 2000-03-24 | 2002-05-29 | Smithkline Beecham Corp | Method of amplifying quinolone-resistance-determining-regions and identifying polymorphic variants thereof |
| EP2278030B1 (en) | 2000-04-10 | 2017-05-24 | Taxon Biosciences, Inc. | Methods for the survey and genetic analysis of populations |
| US20030207300A1 (en) | 2000-04-28 | 2003-11-06 | Matray Tracy J. | Multiplex analytical platform using molecular tags |
| US7630063B2 (en) | 2000-08-02 | 2009-12-08 | Honeywell International Inc. | Miniaturized cytometer for detecting multiple species in a sample |
| ATE377093T1 (de) | 2000-07-07 | 2007-11-15 | Visigen Biotechnologies Inc | Sequenzbestimmung in echtzeit |
| DE60131903T2 (de) | 2000-10-24 | 2008-11-27 | The Board of Trustees of the Leland S. Stanford Junior University, Palo Alto | Direkte multiplex charakterisierung von genomischer dna |
| US20020142345A1 (en) | 2000-12-22 | 2002-10-03 | Nelsen Anita J. | Methods for encoding and decoding complex mixtures in arrayed assays |
| US20030049616A1 (en) | 2001-01-08 | 2003-03-13 | Sydney Brenner | Enzymatic synthesis of oligonucleotide tags |
| WO2002070684A2 (en) | 2001-01-11 | 2002-09-12 | Lion Bioscience Ag | Gene library for screening methods |
| ES2382542T3 (es) * | 2001-01-25 | 2012-06-11 | Luminex Molecular Diagnostics, Inc. | Polinucleótidos para su utilización como etiquetas y complementos de etiqueta, fabricación y utilización de los mismos |
| US20030165865A1 (en) | 2001-01-29 | 2003-09-04 | Hinkel Christopher A. | Methods of analysis of nucleic acids |
| JP2005233974A (ja) | 2001-03-21 | 2005-09-02 | Olympus Corp | 生化学的検査方法 |
| US7027932B2 (en) | 2001-03-21 | 2006-04-11 | Olympus Optical Co., Ltd. | Biochemical examination method |
| AUPR480901A0 (en) | 2001-05-04 | 2001-05-31 | Genomics Research Partners Pty Ltd | Diagnostic method for assessing a condition of a performance animal |
| US6849404B2 (en) | 2001-05-07 | 2005-02-01 | Bioneer Corporation | Polymerase chain reaction of DNA of which base sequence is completely unidentified |
| US6905827B2 (en) | 2001-06-08 | 2005-06-14 | Expression Diagnostics, Inc. | Methods and compositions for diagnosing or monitoring auto immune and chronic inflammatory diseases |
| JP4244534B2 (ja) | 2001-06-12 | 2009-03-25 | 横河電機株式会社 | バイオチップ |
| US7297494B2 (en) | 2001-06-25 | 2007-11-20 | Georgia Tech Research Corporation | Activatable probes and methods for in vivo gene detection |
| US7473767B2 (en) | 2001-07-03 | 2009-01-06 | The Institute For Systems Biology | Methods for detection and quantification of analytes in complex mixtures |
| US6830931B2 (en) | 2001-07-12 | 2004-12-14 | Automated Cell, Inc. | Method and apparatus for monitoring of proteins and cells |
| WO2003033741A1 (en) | 2001-10-16 | 2003-04-24 | Aclara Biosciences, Inc. | Universal e-tag primer and probe compositions and methods |
| JP2005515792A (ja) | 2002-01-29 | 2005-06-02 | グローバル ジェノミクス アクティエボラーグ | 核酸を操作するための方法および手段 |
| US20030186251A1 (en) | 2002-04-01 | 2003-10-02 | Brookhaven Science Associates, Llc | Genome sequence tags |
| US20070178478A1 (en) | 2002-05-08 | 2007-08-02 | Dhallan Ravinder S | Methods for detection of genetic disorders |
| US20040096368A1 (en) | 2002-06-28 | 2004-05-20 | Igen International, Inc. | Assay systems and components |
| US20050019776A1 (en) | 2002-06-28 | 2005-01-27 | Callow Matthew James | Universal selective genome amplification and universal genotyping system |
| US7192700B2 (en) | 2002-12-20 | 2007-03-20 | Orchid Cellmark Inc. | Methods and compositions for conducting primer extension and polymorphism detection reactions |
| EP2315027A1 (en) | 2002-08-16 | 2011-04-27 | Decision Biomarkers Incorporated | Substrates for isolating, reacting and microscopically analyzing materials |
| US7361821B2 (en) | 2002-09-20 | 2008-04-22 | Intel Corporation | Controlled alignment of nanobarcodes encoding specific information for scanning probe microscopy (SPM) reading |
| DE60229890D1 (de) | 2002-09-30 | 2008-12-24 | Hoffmann La Roche | Oligonukleotide zur genotypisierung des thymidylat-synthase gens |
| WO2004040001A2 (en) | 2002-10-02 | 2004-05-13 | California Institute Of Technology | Microfluidic nucleic acid analysis |
| EP2418287B1 (en) | 2002-10-11 | 2013-09-18 | Erasmus Universiteit Rotterdam | Nucleic acid amplification primers for PCR-based clonality studies of the TCR-beta gene |
| US7822555B2 (en) | 2002-11-11 | 2010-10-26 | Affymetrix, Inc. | Methods for identifying DNA copy number changes |
| EP1578994A2 (en) | 2002-11-11 | 2005-09-28 | Affymetrix, Inc. | Methods for identifying dna copy number changes |
| US7704687B2 (en) | 2002-11-15 | 2010-04-27 | The Johns Hopkins University | Digital karyotyping |
| US7575865B2 (en) | 2003-01-29 | 2009-08-18 | 454 Life Sciences Corporation | Methods of amplifying and sequencing nucleic acids |
| ATE331044T1 (de) | 2003-03-04 | 2006-07-15 | Pamgene Bv | Verfahren zur integrierten integritätsbewertung und analyse von nukleinsäuren |
| US8067205B2 (en) | 2003-03-28 | 2011-11-29 | Japan As Represented By Director General Of National Rehabilitation Center For Persons With Disabilities | Method of synthesizing cDNA |
| WO2006102264A1 (en) | 2005-03-18 | 2006-09-28 | Fluidigm Corporation | Thermal reaction device and method for using the same |
| US7269518B2 (en) | 2003-04-30 | 2007-09-11 | Agilent Technologies, Inc. | Chemical array reading |
| US7202036B2 (en) | 2003-06-12 | 2007-04-10 | Los Alamos National Security, Llc | Quenching methods for background reduction in luminescence-based probe-target binding assays |
| US20040259118A1 (en) | 2003-06-23 | 2004-12-23 | Macevicz Stephen C. | Methods and compositions for nucleic acid sequence analysis |
| US8048627B2 (en) | 2003-07-05 | 2011-11-01 | The Johns Hopkins University | Method and compositions for detection and enumeration of genetic variations |
| GB0319332D0 (en) | 2003-08-16 | 2003-09-17 | Astrazeneca Ab | Amplification |
| US20050048498A1 (en) | 2003-08-29 | 2005-03-03 | Applera Corporation | Compositions, methods, and kits for assembling probes |
| US7354706B2 (en) | 2003-09-09 | 2008-04-08 | The Regents Of The University Of Colorado, A Body Corporate | Use of photopolymerization for amplification and detection of a molecular recognition event |
| AU2004286201B2 (en) | 2003-09-10 | 2010-09-09 | Altheadx, Inc. | Expression profiling using microarrays |
| US20060024690A1 (en) | 2003-09-19 | 2006-02-02 | Kao H P | Normalization of data using controls |
| WO2005047521A2 (en) | 2003-11-10 | 2005-05-26 | Investigen, Inc. | Methods of preparing nucleic acid for detection |
| WO2005071110A2 (en) | 2004-01-23 | 2005-08-04 | Lingvitae As | Improving polynucleotide ligation reactions |
| WO2005080604A2 (en) | 2004-02-12 | 2005-09-01 | Compass Genetics, Llc | Genetic analysis by sequence-specific sorting |
| US20100216153A1 (en) | 2004-02-27 | 2010-08-26 | Helicos Biosciences Corporation | Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities |
| WO2005111242A2 (en) | 2004-05-10 | 2005-11-24 | Parallele Bioscience, Inc. | Digital profiling of polynucleotide populations |
| AU2005252242A1 (en) | 2004-06-07 | 2005-12-22 | Irm Llc | Dispensing systems, software, and related methods |
| US20060035258A1 (en) | 2004-08-06 | 2006-02-16 | Affymetrix, Inc. | Methods for identifying DNA copy number changes |
| US20060041385A1 (en) | 2004-08-18 | 2006-02-23 | Bauer Kenneth D | Method of quantitating proteins and genes in cells using a combination of immunohistochemistry and in situ hybridization |
| US7713697B2 (en) | 2004-08-27 | 2010-05-11 | Gen-Probe Incorporated | Methods and kits for amplifying DNA |
| US20060073506A1 (en) | 2004-09-17 | 2006-04-06 | Affymetrix, Inc. | Methods for identifying biological samples |
| WO2006071770A2 (en) | 2004-12-23 | 2006-07-06 | I-Stat Corporation | Molecular diagnostics system and methods |
| EP1836302B1 (en) | 2004-12-23 | 2010-04-14 | Amersham Biosciences Corp. | Ligation-based rna amplification |
| US7393665B2 (en) | 2005-02-10 | 2008-07-01 | Population Genetics Technologies Ltd | Methods and compositions for tagging and identifying polynucleotides |
| WO2006085616A1 (ja) | 2005-02-10 | 2006-08-17 | Riken | 核酸配列増幅方法 |
| US20060223122A1 (en) | 2005-03-08 | 2006-10-05 | Agnes Fogo | Classifying and predicting glomerulosclerosis using a proteomics approach |
| WO2006099604A2 (en) | 2005-03-16 | 2006-09-21 | Compass Genetics, Llc | Methods and compositions for assay readouts on multiple analytical platforms |
| GB2424946A (en) | 2005-04-05 | 2006-10-11 | Stratec Biomedical Systems Ag | A detection system for substance binding using up-converting fluorescent probes |
| WO2006116127A2 (en) | 2005-04-21 | 2006-11-02 | Isis Pharmaceuticals, Inc. | Compositions for use in identification of bacteria |
| US20060263789A1 (en) | 2005-05-19 | 2006-11-23 | Robert Kincaid | Unique identifiers for indicating properties associated with entities to which they are attached, and methods for using |
| US7695886B2 (en) | 2005-05-19 | 2010-04-13 | Fuji Xerox Co., Ltd. | Process for producing resin particle liquid dispersion for electrostatic image developing toner, electrostatic image developing toner and production process thereof |
| US8407013B2 (en) | 2005-06-07 | 2013-03-26 | Peter K. Rogan | AB initio generation of single copy genomic probes |
| US7796815B2 (en) | 2005-06-10 | 2010-09-14 | The Cleveland Clinic Foundation | Image analysis of biological objects |
| US20070020640A1 (en) | 2005-07-21 | 2007-01-25 | Mccloskey Megan L | Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis |
| JP2009508495A (ja) | 2005-09-16 | 2009-03-05 | ４５４ ライフ サイエンシーズ コーポレイション | ｃＤＮＡライブラリー調製 |
| US8313711B2 (en) | 2005-11-01 | 2012-11-20 | Freeslate, Inc. | Liquid dispensing for high-throughput experimentation |
| US7375211B2 (en) | 2005-11-18 | 2008-05-20 | Kou Zhong C | Method for detection and quantification of T-cell receptor Vβ repertoire |
| US20080070303A1 (en) | 2005-11-21 | 2008-03-20 | West Michael D | Methods to accelerate the isolation of novel cell strains from pluripotent stem cells and cells obtained thereby |
| US7636465B2 (en) | 2005-12-09 | 2009-12-22 | Cytyc Corporation | Cross-frame object reconstruction for image-based cytology applications |
| WO2007087310A2 (en) | 2006-01-23 | 2007-08-02 | Population Genetics Technologies Ltd. | Nucleic acid analysis using sequence tokens |
| WO2007087312A2 (en) | 2006-01-23 | 2007-08-02 | Population Genetics Technologies Ltd. | Molecular counting |
| US20080038727A1 (en) | 2006-03-10 | 2008-02-14 | Applera Corporation | MicroRNA and Messenger RNA Detection on Arrays |
| US7880142B2 (en) | 2006-04-04 | 2011-02-01 | Sidec Technologies Ab | Extended electron tomography |
| US20080194414A1 (en) | 2006-04-24 | 2008-08-14 | Albert Thomas J | Enrichment and sequence analysis of genomic regions |
| WO2007136874A2 (en) | 2006-05-18 | 2007-11-29 | President And Fellows Of Harvard College | Genomic library construction |
| WO2007147079A2 (en) | 2006-06-14 | 2007-12-21 | Living Microsystems, Inc. | Rare cell analysis using sample splitting and dna tags |
| AU2007284651B2 (en) | 2006-08-09 | 2014-03-20 | Institute For Systems Biology | Organ-specific proteins and methods of their use |
| WO2008147428A1 (en) | 2006-10-05 | 2008-12-04 | Nanopoint, Inc. | Systems and methods for active microfluidic cell handling |
| CA2669728C (en) | 2006-11-15 | 2017-04-11 | Biospherex Llc | Multitag sequencing and ecogenomics analysis |
| US8050476B2 (en) | 2006-11-22 | 2011-11-01 | General Electric Company | Heart rate demodulation of periodic movement in ultrasound data for object identification |
| GB2457851B (en) | 2006-12-14 | 2011-01-05 | Ion Torrent Systems Inc | Methods and apparatus for measuring analytes using large scale fet arrays |
| WO2008087869A1 (ja) | 2007-01-16 | 2008-07-24 | Olympus Corporation | 蛍光信号解析装置および蛍光信号解析方法 |
| US9063133B2 (en) | 2007-01-30 | 2015-06-23 | The Regents Of The University Of California | Methods and devices for biomolecular arrays |
| US20080269068A1 (en) | 2007-02-06 | 2008-10-30 | President And Fellows Of Harvard College | Multiplex decoding of sequence tags in barcodes |
| WO2008096318A2 (en) | 2007-02-09 | 2008-08-14 | Koninklijke Philips Electronics N.V. | Identification system |
| KR100882711B1 (ko) | 2007-03-12 | 2009-02-06 | 성균관대학교산학협력단 | 사이크로박터 스피시스 ｈｊ147 균주 유래의 우라실-ｄｎａ글리코실라제 및 이의 용도 |
| JP2008256428A (ja) | 2007-04-03 | 2008-10-23 | Intec Systems Institute Inc | マイクロアレイ画像のブロック位置検出方法 |
| US20080274458A1 (en) | 2007-05-01 | 2008-11-06 | Latham Gary J | Nucleic acid quantitation methods |
| US20090061513A1 (en) | 2007-05-15 | 2009-03-05 | Picovitro Ab | Cell sorting and cell cultivation methods |
| JP2010528608A (ja) | 2007-06-01 | 2010-08-26 | ４５４ ライフ サイエンシーズ コーポレイション | 複合的な混合物から個々の試料を特定するためのシステムおよび方法 |
| EP2171097A2 (en) | 2007-06-29 | 2010-04-07 | Population Genetics Technologies LTD. | Methods and compositions for isolating nucleic acid sequence variants |
| WO2009105670A2 (en) | 2008-02-21 | 2009-08-27 | Gentel Biosciences, Inc. | Substrates for multiplexed assays and uses thereof |
| US8687189B2 (en) | 2008-03-03 | 2014-04-01 | Ajjer, Llc | Analysis of arrays by laser induced breakdown spectroscopy |
| JP5528333B2 (ja) | 2008-05-08 | 2014-06-25 | 天野エンザイム株式会社 | β−アミラーゼ、それをコードする遺伝子及びその製造法 |
| DE102008025656B4 (de) | 2008-05-28 | 2016-07-28 | Genxpro Gmbh | Verfahren zur quantitativen Analyse von Nukleinsäuren, Marker dafür und deren Verwendung |
| US20090298709A1 (en) | 2008-05-28 | 2009-12-03 | Affymetrix, Inc. | Assays for determining telomere length and repeated sequence copy number |
| WO2009148560A2 (en) | 2008-05-30 | 2009-12-10 | Board Of Regents, The Universtiy Of Texas System | Methods and compositions for nucleic acid sequencing |
| US20100069250A1 (en) | 2008-08-16 | 2010-03-18 | The Board Of Trustees Of The Leland Stanford Junior University | Digital PCR Calibration for High Throughput Sequencing |
| US9896720B2 (en) | 2008-09-10 | 2018-02-20 | Rutgers, The State University Of New Jersey | Imaging individual mRNA molecules using multiple singly labeled probes |
| US8476013B2 (en) | 2008-09-16 | 2013-07-02 | Sequenom, Inc. | Processes and compositions for methylation-based acid enrichment of fetal nucleic acid from a maternal sample useful for non-invasive prenatal diagnoses |
| US20100159533A1 (en) * | 2008-11-24 | 2010-06-24 | Helicos Biosciences Corporation | Simplified sample preparation for rna analysis |
| US8492096B2 (en) | 2008-12-24 | 2013-07-23 | Boris Pasche | TGFBR1 expression modifies risk for colorectal cancer |
| AU2010206843B2 (en) | 2009-01-20 | 2015-01-29 | The Board Of Trustees Of The Leland Stanford Junior University | Single cell gene expression for diagnosis, prognosis and identification of drug targets |
| US20100323348A1 (en) | 2009-01-31 | 2010-12-23 | The Regents Of The University Of Colorado, A Body Corporate | Methods and Compositions for Using Error-Detecting and/or Error-Correcting Barcodes in Nucleic Acid Amplification Process |
| CA2754446A1 (en) | 2009-03-05 | 2010-09-10 | Daiichi Sankyo Company, Limited | Pyridine derivative |
| EP2230312A1 (en) | 2009-03-19 | 2010-09-22 | Helmholtz-Zentrum für Infektionsforschung GmbH | Probe compound for detecting and isolating enzymes and means and methods using the same |
| EP2414548B1 (en) | 2009-03-30 | 2015-10-21 | Illumina, Inc. | Gene expression analysis in single cells |
| SG174617A1 (en) | 2009-04-02 | 2011-10-28 | Fluidigm Corp | Multi-primer amplification method for barcoding of target nucleic acids |
| US9085798B2 (en) | 2009-04-30 | 2015-07-21 | Prognosys Biosciences, Inc. | Nucleic acid constructs and methods of use |
| FR2945545B1 (fr) | 2009-05-14 | 2011-08-05 | Univ Aix Marseille Ii | Methode de detection d'adn procaryote extrait d'un echantillon de selles |
| US8673627B2 (en) | 2009-05-29 | 2014-03-18 | Life Technologies Corporation | Apparatus and methods for performing electrochemical reactions |
| GB0909923D0 (en) | 2009-06-09 | 2009-07-22 | Oxford Gene Tech Ip Ltd | Picowell capture devices for analysing single cells or other particles |
| US9587270B2 (en) | 2009-06-29 | 2017-03-07 | Luminex Corporation | Chimeric primers with hairpin conformations and methods of using same |
| US8330957B2 (en) | 2009-06-29 | 2012-12-11 | Hager Enviromental and Atmospheric Technologies, LLC | Device and method for quantification of gases in plumes by remote sensing |
| EP3029141A1 (en) | 2009-08-20 | 2016-06-08 | Population Genetics Technologies Ltd. | Compositions and methods for intramolecular nucleic acid rearrangement |
| WO2011027268A2 (en) | 2009-09-01 | 2011-03-10 | Koninklijke Philips Electronics N.V. | Devices and methods for microarray selection |
| WO2011028818A2 (en) | 2009-09-01 | 2011-03-10 | Trustees Of Boston University | High throughput multichannel reader and uses thereof |
| US9625454B2 (en) | 2009-09-04 | 2017-04-18 | The Research Foundation For The State University Of New York | Rapid and continuous analyte processing in droplet microfluidic devices |
| WO2011041308A1 (en) | 2009-09-30 | 2011-04-07 | Quest Diagnostics Investments Incorporated | Bcr-abl truncation mutations |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| US9315857B2 (en) | 2009-12-15 | 2016-04-19 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse label-tags |
| EP2370599B1 (en) | 2010-01-19 | 2015-01-21 | Verinata Health, Inc | Method for determining copy number variations |
| EP2513341B1 (en) | 2010-01-19 | 2017-04-12 | Verinata Health, Inc | Identification of polymorphic sequences in mixtures of genomic dna by whole genome sequencing |
| CN102725424B (zh) | 2010-01-25 | 2014-07-09 | Rd生物科技公司 | 靶核酸的自折叠扩增 |
| EP2531608B1 (en) | 2010-02-05 | 2020-08-19 | Siemens Healthcare Diagnostics Inc. | Methods for increasing multiplex level by externalization of passive reference in polymerase chain reactions |
| US9494520B2 (en) | 2010-02-12 | 2016-11-15 | Raindance Technologies, Inc. | Digital analyte analysis |
| EP4484577A3 (en) | 2010-02-12 | 2025-03-26 | Bio-Rad Laboratories, Inc. | Digital analyte analysis |
| JP5901046B2 (ja) | 2010-02-19 | 2016-04-06 | 国立大学法人 千葉大学 | ＯＡＴＰ１Ｂ３ｍＲＮＡの新規な選択的スプライシングバリアント |
| ES2623859T3 (es) | 2010-03-04 | 2017-07-12 | Miacom Diagnostics Gmbh | FISH múltiple mejorada |
| US8951940B2 (en) | 2010-04-01 | 2015-02-10 | Illumina, Inc. | Solid-phase clonal amplification and related methods |
| WO2011143659A2 (en) | 2010-05-14 | 2011-11-17 | Fluidigm Corporation | Nucleic acid isolation methods |
| US8828688B2 (en) | 2010-05-27 | 2014-09-09 | Affymetrix, Inc. | Multiplex amplification methods |
| US9080210B2 (en) | 2010-06-09 | 2015-07-14 | Keygene N.V. | High throughput screening using combinatorial sequence barcodes |
| EP2407242A1 (en) | 2010-07-13 | 2012-01-18 | Dublin City University | Direct clone analysis and selection technology |
| US20120040843A1 (en) | 2010-08-12 | 2012-02-16 | Dublin City University | Centrifugal capture system |
| ES2523140T3 (es) | 2010-09-21 | 2014-11-21 | Population Genetics Technologies Ltd. | Aumento de la confianza en las identificaciones de alelos con el recuento molecular |
| WO2012042374A2 (en) | 2010-10-01 | 2012-04-05 | Anssi Jussi Nikolai Taipale | Method of determining number or concentration of molecules |
| US20120088691A1 (en) | 2010-10-08 | 2012-04-12 | Gao Chen | Highly multiplexed real-time pcr using encoded microbeads |
| GB2497912B (en) | 2010-10-08 | 2014-06-04 | Harvard College | High-throughput single cell barcoding |
| CA3210003A1 (en) * | 2010-10-22 | 2012-04-26 | Cold Spring Harbor Laboratory | Varietal counting of nucleic acids for obtaining genomic copy number information |
| US20130225623A1 (en) | 2010-10-27 | 2013-08-29 | Mount Sinai School Of Medicine | Methods of Treating Psychiatric or Neurological Disorders with MGLUR Antagonists |
| WO2012075028A1 (en) | 2010-11-29 | 2012-06-07 | Dako Denmark A/S | Methods and systems for analyzing images of specimens processed by a programmable quantitative assay |
| US9394511B2 (en) | 2010-12-05 | 2016-07-19 | Wenbin Jiang | Rapid single cell based parallel biological cell sorter |
| CN107159328B (zh) | 2010-12-09 | 2020-04-14 | 阿科尼生物系统公司 | 样本分析系统 |
| WO2012083225A2 (en) | 2010-12-16 | 2012-06-21 | Gigagen, Inc. | System and methods for massively parallel analysis of nycleic acids in single cells |
| US10241075B2 (en) | 2010-12-30 | 2019-03-26 | Life Technologies Corporation | Methods, systems, and computer readable media for nucleic acid sequencing |
| US20120190020A1 (en) | 2011-01-25 | 2012-07-26 | Aria Diagnostics, Inc. | Detection of genetic abnormalities |
| JP6247934B2 (ja) | 2011-02-04 | 2017-12-13 | ザ トラスティーズ オブ ザ ユニバーシティ オブ ペンシルバニア | 単一細胞において染色体構造および遺伝子発現を同時に検出するための方法 |
| US9365897B2 (en) | 2011-02-08 | 2016-06-14 | Illumina, Inc. | Selective enrichment of nucleic acids |
| WO2012112804A1 (en) | 2011-02-18 | 2012-08-23 | Raindance Technoligies, Inc. | Compositions and methods for molecular labeling |
| WO2012129363A2 (en) | 2011-03-24 | 2012-09-27 | President And Fellows Of Harvard College | Single cell nucleic acid detection and analysis |
| KR20120110431A (ko) | 2011-03-29 | 2012-10-10 | 에스케이하이닉스 주식회사 | 반도체 메모리 장치 |
| GB201106254D0 (en) | 2011-04-13 | 2011-05-25 | Frisen Jonas | Method and product |
| ES2986436T3 (es) | 2011-04-15 | 2024-11-11 | Univ Johns Hopkins | Sistema de secuenciación segura |
| EP3395957B1 (en) | 2011-04-25 | 2020-08-12 | Bio-Rad Laboratories, Inc. | Methods and compositions for nucleic acid analysis |
| SG10201605049QA (en) | 2011-05-20 | 2016-07-28 | Fluidigm Corp | Nucleic acid encoding reactions |
| WO2012162161A1 (en) | 2011-05-20 | 2012-11-29 | Phthisis Diagnostics | Microsporidia detection system and method |
| PL3892295T3 (pl) | 2011-05-24 | 2023-07-24 | BioNTech SE | Zindywidualizowane szczepionki przeciwnowotworowe |
| WO2012162621A1 (en) | 2011-05-26 | 2012-11-29 | Brandeis University | Methods for suppression pcr |
| US8841071B2 (en) | 2011-06-02 | 2014-09-23 | Raindance Technologies, Inc. | Sample multiplexing |
| US9752176B2 (en) | 2011-06-15 | 2017-09-05 | Ginkgo Bioworks, Inc. | Methods for preparative in vitro cloning |
| EP2724160A2 (en) | 2011-06-27 | 2014-04-30 | Life Technologies Corporation | Acoustic cytometry methods and protocols |
| KR101454886B1 (ko) | 2011-08-01 | 2014-11-03 | 주식회사 셀레믹스 | 핵산분자의 제조방법 |
| US10364464B2 (en) | 2011-08-08 | 2019-07-30 | The Broad Institute, Inc. | Compositions and methods for co-amplifying subsequences of a nucleic acid fragment sequence |
| JP5816486B2 (ja) | 2011-08-18 | 2015-11-18 | オリンパス株式会社 | 蛍光観察装置および蛍光観察システム並びに蛍光観察装置の蛍光画像処理方法 |
| KR101337094B1 (ko) | 2011-11-30 | 2013-12-05 | 삼성에스디에스 주식회사 | 염기 서열 정렬 장치 및 그 방법 |
| WO2013117595A2 (en) | 2012-02-07 | 2013-08-15 | Illumina Cambridge Limited | Targeted enrichment and amplification of nucleic acids on a support |
| WO2013120089A1 (en) | 2012-02-10 | 2013-08-15 | Raindance Technologies, Inc. | Molecular diagnostic screening assay |
| EP3309262B1 (en) | 2012-02-24 | 2019-09-25 | Bio-Rad Laboratories, Inc. | Labeling and sample preparation for sequencing |
| CA2865575C (en) | 2012-02-27 | 2024-01-16 | Cellular Research, Inc. | Compositions and kits for molecular counting |
| EP2820174B1 (en) | 2012-02-27 | 2019-12-25 | The University of North Carolina at Chapel Hill | Methods and uses for molecular tags |
| US9803239B2 (en) | 2012-03-29 | 2017-10-31 | Complete Genomics, Inc. | Flow cells for high density array chips |
| HUE029357T2 (en) * | 2012-05-08 | 2017-02-28 | Adaptive Biotechnologies Corp | Preparations and devices for measuring and calibrating amplification distortion in multiplex PCR reactions |
| PT2850211T (pt) | 2012-05-14 | 2021-11-29 | Irepertoire Inc | Método para aumentar a precisão na deteção quantitativa de polinucleótidos |
| WO2013177206A2 (en) | 2012-05-21 | 2013-11-28 | Fluidigm Corporation | Single-particle analysis of particle populations |
| JP6200948B2 (ja) | 2012-05-25 | 2017-09-20 | ザ・ユニヴァーシティ・オヴ・ノース・キャロライナ・アト・チャペル・ヒル | マイクロ流体デバイス |
| SG11201408128WA (en) | 2012-06-15 | 2015-01-29 | Adaptive Biotechnologies Corp | Uniquely tagged rearranged adaptive immune receptor genes in a complex gene set |
| EP2861760B1 (en) | 2012-06-15 | 2020-01-01 | The Board of Regents of The University of Texas System | High throughput sequencing of multiple transcripts of a single cell |
| CA2877094A1 (en) | 2012-06-18 | 2013-12-27 | Nugen Technologies, Inc. | Compositions and methods for negative selection of non-desired nucleic acid sequences |
| CA2876505A1 (en) | 2012-07-17 | 2014-01-23 | Counsyl, Inc. | System and methods for detecting genetic variation |
| WO2014015098A1 (en) | 2012-07-18 | 2014-01-23 | Siemens Healthcare Diagnostics Inc. | A method of normalizing biological samples |
| EP2877604A1 (en) | 2012-07-24 | 2015-06-03 | Sequenta, Inc. | Single cell analysis using sequence tags |
| EP2882868B1 (en) | 2012-08-08 | 2019-07-31 | H. Hoffnabb-La Roche Ag | Increasing dynamic range for identifying multiple epitopes in cells |
| US20140378345A1 (en) | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US20140378322A1 (en) | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US20140378349A1 (en) | 2012-08-14 | 2014-12-25 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US10221442B2 (en) | 2012-08-14 | 2019-03-05 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US20150005200A1 (en) | 2012-08-14 | 2015-01-01 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| AU2013302756C1 (en) | 2012-08-14 | 2018-05-17 | 10X Genomics, Inc. | Microcapsule compositions and methods |
| US20150005199A1 (en) | 2012-08-14 | 2015-01-01 | 10X Technologies, Inc. | Compositions and methods for sample processing |
| US9951386B2 (en) | 2014-06-26 | 2018-04-24 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| WO2014071361A1 (en) | 2012-11-05 | 2014-05-08 | Rubicon Genomics | Barcoding nucleic acids |
| CA2894694C (en) | 2012-12-14 | 2023-04-25 | 10X Genomics, Inc. | Methods and systems for processing polynucleotides |
| US9683230B2 (en) | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
| WO2014108850A2 (en) | 2013-01-09 | 2014-07-17 | Yeda Research And Development Co. Ltd. | High throughput transcriptome analysis |
| US9562269B2 (en) | 2013-01-22 | 2017-02-07 | The Board Of Trustees Of The Leland Stanford Junior University | Haplotying of HLA loci with ultra-deep shotgun sequencing |
| CN104955576A (zh) | 2013-01-24 | 2015-09-30 | 沙特基础全球技术有限公司 | 由聚酯-聚碳酸酯制成的微孔板 |
| US10190963B2 (en) | 2013-02-01 | 2019-01-29 | Becton, Dickinson And Company | Methods and systems for assessing sample behavior in a flow cytometer |
| KR20200140929A (ko) | 2013-02-08 | 2020-12-16 | 10엑스 제노믹스, 인크. | 폴리뉴클레오티드 바코드 생성 |
| WO2014126937A1 (en) | 2013-02-14 | 2014-08-21 | The Board Of Trustees Of The Leland Stanford Junior University | Suspension arrays and multiplexed assays based thereon |
| US20140274811A1 (en) | 2013-03-14 | 2014-09-18 | Lyle J. Arnold | Methods for Amplifying a Complete Genome or Transcriptome |
| GB2525568B (en) | 2013-03-15 | 2020-10-14 | Abvitro Llc | Single cell barcoding for antibody discovery |
| KR20150132479A (ko) * | 2013-03-15 | 2015-11-25 | 어댑티브 바이오테크놀로지스 코포레이션 | 복합 유전자 세트에서의 독특하게 태그되고 재배열된 적응 면역 수용체 유전자 |
| US20140303005A1 (en) | 2013-04-05 | 2014-10-09 | Raindance Technologies, Inc. | Rare cell analysis after negative selection |
| EP2805769A1 (en) | 2013-05-24 | 2014-11-26 | European Molecular Biology Laboratory | Methods for nano-scale single cell analysis |
| WO2014201273A1 (en) | 2013-06-12 | 2014-12-18 | The Broad Institute, Inc. | High-throughput rna-seq |
| AU2014302277A1 (en) | 2013-06-27 | 2015-12-24 | 10X Genomics, Inc. | Compositions and methods for sample processing |
| US9708657B2 (en) | 2013-07-01 | 2017-07-18 | Adaptive Biotechnologies Corp. | Method for generating clonotype profiles using sequence tags |
| KR102758333B1 (ko) | 2013-08-28 | 2025-01-23 | 벡톤 디킨슨 앤드 컴퍼니 | 대량의 동시 단일 세포 분석 |
| US10395758B2 (en) | 2013-08-30 | 2019-08-27 | 10X Genomics, Inc. | Sequencing methods |
| EP3041957A4 (en) | 2013-09-04 | 2017-03-29 | Fluidigm Corporation | Proximity assays for detecting nucleic acids and proteins in a single cell |
| GB201317301D0 (en) | 2013-09-30 | 2013-11-13 | Linnarsson Sten | Method for capturing and encoding nucleic acid from a plurality of single cells |
| CN105745334B (zh) | 2013-09-30 | 2019-12-10 | 吴迪 | 通过使用邻近条形编码分析分子复合物的概况的方法 |
| US9582877B2 (en) | 2013-10-07 | 2017-02-28 | Cellular Research, Inc. | Methods and systems for digitally counting features on arrays |
| DK3089822T3 (da) | 2013-12-30 | 2022-05-02 | Atreca Inc | Analyse af nukleinsyrer associeret med enkelte celler under anvendelse af nukleinsyrestregkoder |
| WO2015134787A2 (en) | 2014-03-05 | 2015-09-11 | Adaptive Biotechnologies Corporation | Methods using randomer-containing synthetic molecules |
| US20150298091A1 (en) | 2014-04-21 | 2015-10-22 | President And Fellows Of Harvard College | Systems and methods for barcoding nucleic acids |
| CA2953469A1 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Analysis of nucleic acid sequences |
| WO2015200893A2 (en) | 2014-06-26 | 2015-12-30 | 10X Genomics, Inc. | Methods of analyzing nucleic acids from individual cells or cell populations |
| EP3248018B1 (en) | 2015-01-22 | 2020-01-08 | Becton, Dickinson and Company | Devices and systems for molecular barcoding of nucleic acid targets in single cells |
| US10760120B2 (en) | 2015-01-23 | 2020-09-01 | Qiagen Sciences, Llc | High multiplex PCR with molecular barcoding |
| EP3259371B1 (en) | 2015-02-19 | 2020-09-02 | Becton, Dickinson and Company | High-throughput single-cell analysis combining proteomic and genomic information |
| WO2016138500A1 (en) * | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Methods and compositions for barcoding nucleic acids for sequencing |
| WO2016138496A1 (en) | 2015-02-27 | 2016-09-01 | Cellular Research, Inc. | Spatially addressable molecular barcoding |
| WO2016160844A2 (en) | 2015-03-30 | 2016-10-06 | Cellular Research, Inc. | Methods and compositions for combinatorial barcoding |
| EP4582556A3 (en) | 2015-04-23 | 2025-10-08 | Becton, Dickinson and Company | Methods and compositions for whole transcriptome amplification |
| US9618871B2 (en) | 2015-04-28 | 2017-04-11 | Kyocera Document Solutions Inc. | Image forming apparatus |
| WO2016196229A1 (en) | 2015-06-01 | 2016-12-08 | Cellular Research, Inc. | Methods for rna quantification |
| JP6940484B2 (ja) | 2015-09-11 | 2021-09-29 | セルラー リサーチ， インコーポレイテッド | ライブラリー正規化のための方法および組成物 |
| JP7129343B2 (ja) | 2016-05-02 | 2022-09-01 | ベクトン・ディキンソン・アンド・カンパニー | 正確な分子バーコーディング |
| ES2979395T3 (es) | 2016-05-26 | 2024-09-25 | Becton Dickinson Co | Métodos de ajuste del recuento de etiquetas moleculares |
| TWI600309B (zh) | 2016-05-28 | 2017-09-21 | Hon Hai Prec Ind Co Ltd | 角度調整機構 |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| US20180037942A1 (en) | 2016-08-03 | 2018-02-08 | Cellular Research, Inc. | Enzyme-independent molecular indexing |
| CN109791157B (zh) | 2016-09-26 | 2022-06-07 | 贝克顿迪金森公司 | 使用具有条形码化的寡核苷酸序列的试剂测量蛋白质表达 |
| JP7228510B2 (ja) | 2016-11-08 | 2023-02-24 | ベクトン・ディキンソン・アンド・カンパニー | 細胞標識分類の方法 |
| IL266681B2 (en) * | 2016-11-18 | 2025-01-01 | Twist Bioscience Corp | Polynucleotide libraries with controlled stoichiometry and their synthesis |
| US20190177800A1 (en) | 2017-12-08 | 2019-06-13 | 10X Genomics, Inc. | Methods and compositions for labeling cells |
| CN110234772B (zh) | 2017-01-31 | 2024-01-19 | 路德维格癌症研究所有限公司 | 增强的免疫细胞受体测序方法 |
| CN117887804A (zh) | 2017-02-02 | 2024-04-16 | 纽约基因组研究中心公司 | 用于识别或量化在生物样品中的靶标的方法和组合物 |
| CN110719959B (zh) | 2017-06-05 | 2021-08-06 | 贝克顿迪金森公司 | 针对单细胞的样品索引 |
| WO2019084046A1 (en) | 2017-10-23 | 2019-05-02 | The Broad Institute, Inc. | ENRICHING SINGLE CELL CELLULAR CONSTITUENT OF BARCODE SEQUENCING LIBRARY |
| WO2019084055A1 (en) | 2017-10-23 | 2019-05-02 | Massachusetts Institute Of Technology | CLASSIFICATION OF GENETIC VARIATION FROM UNICELLULAR TRANSCRIPTOMS |
| US20220062394A1 (en) | 2018-12-17 | 2022-03-03 | The Broad Institute, Inc. | Methods for identifying neoantigens |
| US11371076B2 (en) | 2019-01-16 | 2022-06-28 | Becton, Dickinson And Company | Polymerase chain reaction normalization through primer titration |
| US20200255888A1 (en) | 2019-02-12 | 2020-08-13 | Becton, Dickinson And Company | Determining expressions of transcript variants and polyadenylation sites |
-
2017
- 2017-04-28 JP JP2018557413A patent/JP7129343B2/ja active Active
- 2017-04-28 WO PCT/US2017/030097 patent/WO2017192387A1/en not_active Ceased
- 2017-04-28 US US15/581,914 patent/US10822643B2/en not_active Ceased
- 2017-04-28 EP EP17735675.5A patent/EP3452614B1/en active Active
- 2017-04-28 CA CA3019589A patent/CA3019589A1/en active Pending
- 2017-04-28 EP EP23181147.2A patent/EP4269616A3/en active Pending
- 2017-04-28 ES ES17735675T patent/ES2956757T3/es active Active
- 2017-04-28 AU AU2017261189A patent/AU2017261189B2/en active Active
- 2017-04-28 CN CN201780026011.9A patent/CN109072288A/zh active Pending
-
2022
- 2022-06-30 JP JP2022106377A patent/JP7607614B2/ja active Active
- 2022-10-27 US US18/050,449 patent/USRE50636E1/en active Active
-
2023
- 2023-05-03 AU AU2023202747A patent/AU2023202747B2/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| AU2023202747A1 (en) | 2023-05-25 |
| JP2022153390A (ja) | 2022-10-12 |
| USRE50636E1 (en) | 2025-10-14 |
| JP7607614B2 (ja) | 2024-12-27 |
| EP3452614B1 (en) | 2023-06-28 |
| JP2019520046A (ja) | 2019-07-18 |
| US20170314067A1 (en) | 2017-11-02 |
| EP4269616A3 (en) | 2024-02-14 |
| AU2023202747B2 (en) | 2025-09-04 |
| ES2956757T3 (es) | 2023-12-27 |
| WO2017192387A1 (en) | 2017-11-09 |
| US10822643B2 (en) | 2020-11-03 |
| AU2017261189A1 (en) | 2018-10-11 |
| CN109072288A (zh) | 2018-12-21 |
| AU2017261189B2 (en) | 2023-02-09 |
| EP4269616A2 (en) | 2023-11-01 |
| EP3452614A1 (en) | 2019-03-13 |
| JP7129343B2 (ja) | 2022-09-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| USRE50636E1 (en) | Accurate molecular barcoding | |
| EP3861134B1 (en) | Determining 5' transcript sequences | |
| CN113574178B (zh) | 与抗体关联的寡核苷酸 | |
| JP7584420B2 (ja) | 相補的ｄｎａを調製するための方法およびキット | |
| EP4407041A2 (en) | Using random priming to obtain full-length v(d)j information for immune repertoire sequencing | |
| DK2374900T3 (en) | Polynucleotides for amplification and analysis of the total genomic and total transcription libraries generated by a DNA polymerization | |
| US20250257350A1 (en) | Integrative DNA and RNA Library Preparations and Uses Thereof | |
| US9970048B2 (en) | Oligonucleotides and methods for the preparation of RNA libraries | |
| US12545950B2 (en) | Methods for preparing CDNA samples for RNA sequencing, and CDNA samples and uses thereof | |
| EP4256077A1 (en) | Whole transcriptome analysis in single cells | |
| WO2025059808A1 (zh) | 用于高通量标记细胞核酸分子的方法和试剂盒 | |
| US20240384336A1 (en) | Optimized Set Of Oligonucleotides For Bulk RNA Barcoding And Sequencing | |
| EP4536854A1 (en) | Optimised set of oligonucleotides for bulk rna barcoding and sequencing | |
| HK40117221A (zh) | 用於高通量标记细胞核酸分子的方法和试剂盒 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20220309 |
|
| D15 | Examination report completed |
Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D15-D126 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: EXAMINER'S REPORT Effective date: 20240805 |
|
| P11 | Amendment of application requested |
Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P100 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT RECEIVED - RESPONSE TO EXAMINER'S REQUISITION Effective date: 20241204 |
|
| W00 | Other event occurred |
Free format text: ST27 STATUS EVENT CODE: A-2-2-W10-W00-W111 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: CORRESPONDENT DETERMINED COMPLIANT Effective date: 20250206 |
|
| MFA | Maintenance fee for application paid |
Free format text: FEE DESCRIPTION TEXT: MF (APPLICATION, 8TH ANNIV.) - STANDARD Year of fee payment: 8 |
|
| U00 | Fee paid |
Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U00-U101 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE REQUEST RECEIVED Effective date: 20250324 |
|
| U11 | Full renewal or maintenance fee paid |
Free format text: ST27 STATUS EVENT CODE: A-2-2-U10-U11-U102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: MAINTENANCE FEE PAYMENT PAID IN FULL Effective date: 20251003 |
|
| P11 | Amendment of application requested |
Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P11-P102 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: AMENDMENT DETERMINED COMPLIANT Effective date: 20251024 |
|
| P13 | Application amended |
Free format text: ST27 STATUS EVENT CODE: A-2-2-P10-P13-X000 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: APPLICATION AMENDED Effective date: 20251024 |
|
| D15 | Examination report completed |
Free format text: ST27 STATUS EVENT CODE: A-2-2-D10-D15-D126 (AS PROVIDED BY THE NATIONAL OFFICE); EVENT TEXT: EXAMINER'S REPORT Effective date: 20251224 |