CA2957776C - Pretreatment method and nucleic-acid-extracting kit usable for the same - Google Patents
Pretreatment method and nucleic-acid-extracting kit usable for the same Download PDFInfo
- Publication number
- CA2957776C CA2957776C CA2957776A CA2957776A CA2957776C CA 2957776 C CA2957776 C CA 2957776C CA 2957776 A CA2957776 A CA 2957776A CA 2957776 A CA2957776 A CA 2957776A CA 2957776 C CA2957776 C CA 2957776C
- Authority
- CA
- Canada
- Prior art keywords
- nucleic
- acid
- silica particles
- filtering material
- sample
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
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- 108020004707 nucleic acids Proteins 0.000 claims abstract description 205
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- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 205
- 238000000034 method Methods 0.000 claims abstract description 181
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 159
- 230000008569 process Effects 0.000 claims abstract description 95
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- 238000010828 elution Methods 0.000 claims abstract description 13
- 238000001035 drying Methods 0.000 claims abstract description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 64
- 238000005406 washing Methods 0.000 claims description 56
- 238000010521 absorption reaction Methods 0.000 claims description 48
- 239000008213 purified water Substances 0.000 claims description 39
- 238000002414 normal-phase solid-phase extraction Methods 0.000 claims description 5
- 238000010448 genetic screening Methods 0.000 abstract description 23
- 238000012123 point-of-care testing Methods 0.000 abstract description 18
- 239000000243 solution Substances 0.000 description 54
- 239000000523 sample Substances 0.000 description 47
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- 239000007853 buffer solution Substances 0.000 description 9
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 8
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- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 4
- 210000000170 cell membrane Anatomy 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
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- 102000036639 antigens Human genes 0.000 description 3
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- 102000040650 (ribonucleotides)n+m Human genes 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 241000202934 Mycoplasma pneumoniae Species 0.000 description 2
- 239000007984 Tris EDTA buffer Substances 0.000 description 2
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- 230000035945 sensitivity Effects 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- 241001669680 Dormitator maculatus Species 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 208000001572 Mycoplasma Pneumonia Diseases 0.000 description 1
- 201000008235 Mycoplasma pneumoniae pneumonia Diseases 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
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- 239000011324 bead Substances 0.000 description 1
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- 239000008280 blood Substances 0.000 description 1
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- 239000001913 cellulose Substances 0.000 description 1
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- 238000011033 desalting Methods 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 239000003651 drinking water Substances 0.000 description 1
- 235000020188 drinking water Nutrition 0.000 description 1
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- 239000003365 glass fiber Substances 0.000 description 1
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- 238000003317 immunochromatography Methods 0.000 description 1
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- 238000009434 installation Methods 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
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- 239000012139 lysis buffer Substances 0.000 description 1
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- 239000004745 nonwoven fabric Substances 0.000 description 1
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- 239000013612 plasmid Substances 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
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- 239000004626 polylactic acid Substances 0.000 description 1
- 239000004810 polytetrafluoroethylene Substances 0.000 description 1
- 229920001343 polytetrafluoroethylene Polymers 0.000 description 1
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- 238000012545 processing Methods 0.000 description 1
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- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 229910021487 silica fume Inorganic materials 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
- C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Sampling And Sample Adjustment (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2014-179111 | 2014-09-03 | ||
| JP2014179111A JP6437250B2 (ja) | 2014-09-03 | 2014-09-03 | 前処理方法及びそれに用いられる核酸抽出キット |
| PCT/JP2015/074927 WO2016035812A1 (ja) | 2014-09-03 | 2015-09-02 | 前処理方法及びそれに用いられる核酸抽出キット |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2957776A1 CA2957776A1 (en) | 2016-03-10 |
| CA2957776C true CA2957776C (en) | 2021-01-05 |
Family
ID=55439872
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2957776A Active CA2957776C (en) | 2014-09-03 | 2015-09-02 | Pretreatment method and nucleic-acid-extracting kit usable for the same |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US10316350B2 (enExample) |
| EP (1) | EP3190184B1 (enExample) |
| JP (1) | JP6437250B2 (enExample) |
| KR (1) | KR101897177B1 (enExample) |
| AU (1) | AU2015312884B2 (enExample) |
| BR (1) | BR112017004241B8 (enExample) |
| CA (1) | CA2957776C (enExample) |
| DK (1) | DK3190184T3 (enExample) |
| ES (1) | ES2734068T3 (enExample) |
| MX (1) | MX379186B (enExample) |
| MY (1) | MY177446A (enExample) |
| PH (1) | PH12017500258B1 (enExample) |
| PT (1) | PT3190184T (enExample) |
| WO (1) | WO2016035812A1 (enExample) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3733846A4 (en) * | 2017-12-27 | 2021-10-27 | Toray Industries, Inc. | METHOD FOR RECOVERY OF NUCLEIC ACID |
| JP7588815B2 (ja) | 2020-08-17 | 2024-11-25 | 株式会社ミズホメディー | Ｒｎａろ過器及びｒｎａろ過方法 |
Family Cites Families (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH01192494A (ja) * | 1988-01-27 | 1989-08-02 | Mitsubishi Electric Corp | レーザービームによる抜き金型の製造方法 |
| NL8900725A (nl) | 1989-03-23 | 1990-10-16 | Az Univ Amsterdam | Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur. |
| GB9425138D0 (en) * | 1994-12-12 | 1995-02-08 | Dynal As | Isolation of nucleic acid |
| JPH10155481A (ja) | 1996-06-18 | 1998-06-16 | Rikagaku Kenkyusho | Ｄｎａの回収方法 |
| DE69719409T2 (de) | 1996-06-18 | 2004-01-15 | Rikagaku Kenkyusho | Verfahren zur Reinigung von DNS |
| JP4304348B2 (ja) | 1997-09-22 | 2009-07-29 | 独立行政法人理化学研究所 | Ｄｎａの単離方法 |
| JP3812696B2 (ja) * | 1997-11-17 | 2006-08-23 | 東洋紡績株式会社 | リボ核酸の抽出方法 |
| ATE374837T1 (de) * | 2002-01-08 | 2007-10-15 | Hoffmann La Roche | Verwendung eines silikamaterials bei der amplifikation |
| JP2004201607A (ja) | 2002-12-26 | 2004-07-22 | Asahi Kasei Corp | 核酸吸着固相体上でのｌａｍｐ反応 |
| EP1510577A1 (en) | 2003-08-29 | 2005-03-02 | Qiagen GmbH | Method for magnetic bead isolation of nucleic acids |
| JP4831725B2 (ja) * | 2004-02-13 | 2011-12-07 | 栄研化学株式会社 | 簡易的核酸抽出法 |
| US20060257907A1 (en) | 2005-04-19 | 2006-11-16 | The Regents Of The University Of California | Packed bed for nucleic acid capture and amplification |
| US20110091873A1 (en) | 2009-10-21 | 2011-04-21 | Microfluidic Systems, Inc. | Integrated sample preparation and amplification for nucleic acid detection from biological samples |
| JP6024266B2 (ja) * | 2012-08-01 | 2016-11-16 | セイコーエプソン株式会社 | Ｄｎａの抽出方法 |
-
2014
- 2014-09-03 JP JP2014179111A patent/JP6437250B2/ja active Active
-
2015
- 2015-09-02 ES ES15837848T patent/ES2734068T3/es active Active
- 2015-09-02 MY MYPI2017700659A patent/MY177446A/en unknown
- 2015-09-02 KR KR1020177004470A patent/KR101897177B1/ko active Active
- 2015-09-02 BR BR112017004241A patent/BR112017004241B8/pt active IP Right Grant
- 2015-09-02 PT PT15837848T patent/PT3190184T/pt unknown
- 2015-09-02 MX MX2017002770A patent/MX379186B/es unknown
- 2015-09-02 AU AU2015312884A patent/AU2015312884B2/en active Active
- 2015-09-02 DK DK15837848.9T patent/DK3190184T3/da active
- 2015-09-02 CA CA2957776A patent/CA2957776C/en active Active
- 2015-09-02 EP EP15837848.9A patent/EP3190184B1/en active Active
- 2015-09-02 US US15/505,329 patent/US10316350B2/en active Active
- 2015-09-02 WO PCT/JP2015/074927 patent/WO2016035812A1/ja not_active Ceased
-
2017
- 2017-02-10 PH PH12017500258A patent/PH12017500258B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2016035812A1 (ja) | 2016-03-10 |
| KR20170028443A (ko) | 2017-03-13 |
| ES2734068T3 (es) | 2019-12-04 |
| JP6437250B2 (ja) | 2018-12-12 |
| BR112017004241A2 (pt) | 2017-12-12 |
| MY177446A (en) | 2020-09-15 |
| EP3190184A1 (en) | 2017-07-12 |
| US20170335370A1 (en) | 2017-11-23 |
| AU2015312884A1 (en) | 2017-03-02 |
| BR112017004241B8 (pt) | 2021-07-27 |
| PH12017500258A1 (en) | 2017-07-03 |
| PT3190184T (pt) | 2019-06-27 |
| BR112017004241B1 (pt) | 2020-12-01 |
| EP3190184B1 (en) | 2019-04-10 |
| CA2957776A1 (en) | 2016-03-10 |
| PH12017500258B1 (en) | 2017-07-03 |
| EP3190184A4 (en) | 2018-02-14 |
| AU2015312884B2 (en) | 2018-06-28 |
| KR101897177B1 (ko) | 2018-09-10 |
| MX379186B (es) | 2025-03-10 |
| MX2017002770A (es) | 2017-05-30 |
| US10316350B2 (en) | 2019-06-11 |
| DK3190184T3 (da) | 2019-06-24 |
| JP2016052267A (ja) | 2016-04-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20170209 |
|
| EEER | Examination request |
Effective date: 20170223 |