KR101897177B1 - 전처리 방법 및 그것에 사용되는 핵산 추출 키트 - Google Patents

전처리 방법 및 그것에 사용되는 핵산 추출 키트 Download PDF

Info

Publication number
KR101897177B1
KR101897177B1 KR1020177004470A KR20177004470A KR101897177B1 KR 101897177 B1 KR101897177 B1 KR 101897177B1 KR 1020177004470 A KR1020177004470 A KR 1020177004470A KR 20177004470 A KR20177004470 A KR 20177004470A KR 101897177 B1 KR101897177 B1 KR 101897177B1
Authority
KR
South Korea
Prior art keywords
nucleic acid
silica particles
acid amplification
complex
extraction
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
KR1020177004470A
Other languages
English (en)
Korean (ko)
Other versions
KR20170028443A (ko
Inventor
카즈토미 야마카와
타카시 나가노
Original Assignee
가부시키가이샤 미즈호 메디
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 가부시키가이샤 미즈호 메디 filed Critical 가부시키가이샤 미즈호 메디
Publication of KR20170028443A publication Critical patent/KR20170028443A/ko
Application granted granted Critical
Publication of KR101897177B1 publication Critical patent/KR101897177B1/ko
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Sampling And Sample Adjustment (AREA)
KR1020177004470A 2014-09-03 2015-09-02 전처리 방법 및 그것에 사용되는 핵산 추출 키트 Active KR101897177B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JPJP-P-2014-179111 2014-09-03
JP2014179111A JP6437250B2 (ja) 2014-09-03 2014-09-03 前処理方法及びそれに用いられる核酸抽出キット
PCT/JP2015/074927 WO2016035812A1 (ja) 2014-09-03 2015-09-02 前処理方法及びそれに用いられる核酸抽出キット

Publications (2)

Publication Number Publication Date
KR20170028443A KR20170028443A (ko) 2017-03-13
KR101897177B1 true KR101897177B1 (ko) 2018-09-10

Family

ID=55439872

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020177004470A Active KR101897177B1 (ko) 2014-09-03 2015-09-02 전처리 방법 및 그것에 사용되는 핵산 추출 키트

Country Status (14)

Country Link
US (1) US10316350B2 (enExample)
EP (1) EP3190184B1 (enExample)
JP (1) JP6437250B2 (enExample)
KR (1) KR101897177B1 (enExample)
AU (1) AU2015312884B2 (enExample)
BR (1) BR112017004241B8 (enExample)
CA (1) CA2957776C (enExample)
DK (1) DK3190184T3 (enExample)
ES (1) ES2734068T3 (enExample)
MX (1) MX379186B (enExample)
MY (1) MY177446A (enExample)
PH (1) PH12017500258B1 (enExample)
PT (1) PT3190184T (enExample)
WO (1) WO2016035812A1 (enExample)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3733846A4 (en) * 2017-12-27 2021-10-27 Toray Industries, Inc. METHOD FOR RECOVERY OF NUCLEIC ACID
JP7588815B2 (ja) 2020-08-17 2024-11-25 株式会社ミズホメディー Ｒｎａろ過器及びｒｎａろ過方法

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004201607A (ja) * 2002-12-26 2004-07-22 Asahi Kasei Corp 核酸吸着固相体上でのｌａｍｐ反応

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH01192494A (ja) * 1988-01-27 1989-08-02 Mitsubishi Electric Corp レーザービームによる抜き金型の製造方法
NL8900725A (nl) 1989-03-23 1990-10-16 Az Univ Amsterdam Werkwijze en combinatie van middelen voor het isoleren van nucleinezuur.
GB9425138D0 (en) * 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
JPH10155481A (ja) 1996-06-18 1998-06-16 Rikagaku Kenkyusho Ｄｎａの回収方法
DE69719409T2 (de) 1996-06-18 2004-01-15 Rikagaku Kenkyusho Verfahren zur Reinigung von DNS
JP4304348B2 (ja) 1997-09-22 2009-07-29 独立行政法人理化学研究所 Ｄｎａの単離方法
JP3812696B2 (ja) * 1997-11-17 2006-08-23 東洋紡績株式会社 リボ核酸の抽出方法
ATE374837T1 (de) * 2002-01-08 2007-10-15 Hoffmann La Roche Verwendung eines silikamaterials bei der amplifikation
EP1510577A1 (en) 2003-08-29 2005-03-02 Qiagen GmbH Method for magnetic bead isolation of nucleic acids
JP4831725B2 (ja) * 2004-02-13 2011-12-07 栄研化学株式会社 簡易的核酸抽出法
US20060257907A1 (en) 2005-04-19 2006-11-16 The Regents Of The University Of California Packed bed for nucleic acid capture and amplification
US20110091873A1 (en) 2009-10-21 2011-04-21 Microfluidic Systems, Inc. Integrated sample preparation and amplification for nucleic acid detection from biological samples
JP6024266B2 (ja) * 2012-08-01 2016-11-16 セイコーエプソン株式会社 Ｄｎａの抽出方法

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2004201607A (ja) * 2002-12-26 2004-07-22 Asahi Kasei Corp 核酸吸着固相体上でのｌａｍｐ反応

Also Published As

Publication number Publication date
WO2016035812A1 (ja) 2016-03-10
KR20170028443A (ko) 2017-03-13
ES2734068T3 (es) 2019-12-04
JP6437250B2 (ja) 2018-12-12
BR112017004241A2 (pt) 2017-12-12
CA2957776C (en) 2021-01-05
MY177446A (en) 2020-09-15
EP3190184A1 (en) 2017-07-12
US20170335370A1 (en) 2017-11-23
AU2015312884A1 (en) 2017-03-02
BR112017004241B8 (pt) 2021-07-27
PH12017500258A1 (en) 2017-07-03
PT3190184T (pt) 2019-06-27
BR112017004241B1 (pt) 2020-12-01
EP3190184B1 (en) 2019-04-10
CA2957776A1 (en) 2016-03-10
PH12017500258B1 (en) 2017-07-03
EP3190184A4 (en) 2018-02-14
AU2015312884B2 (en) 2018-06-28
MX379186B (es) 2025-03-10
MX2017002770A (es) 2017-05-30
US10316350B2 (en) 2019-06-11
DK3190184T3 (da) 2019-06-24
JP2016052267A (ja) 2016-04-14

Similar Documents

Publication Publication Date Title
US6746841B1 (en) FTA- coated media for use as a molecular diagnostic tool
JP7354327B2 (ja) 生体液からの細胞外小胞の単離及びセルフリーｄｎａの同時単離のための自動及び手動方法
CA2999521C (en) Liquid to liquid biological particle concentrator with disposable fluid path
CN103415617B (zh) 用于处理样品的装置和方法
US20090043087A1 (en) DNA purification and recovery from high particulate and solids samples
WO2002016383A1 (en) Method for the isolation of nucleic acids and for quantitative dna extraction and detection for leukocyte evaluation in blood products
CA2684311A1 (en) Microorganism detection method and apparatus
CN205115443U (zh) 一种集提取、扩增和检测一体化的基因检测装置
EP1177420B1 (en) Fta-coated media for use as a molecular diagnostic tool
JP6628794B2 (ja) クロマトグラフ濃縮を使用する微生物の検出方法
KR101897177B1 (ko) 전처리 방법 및 그것에 사용되는 핵산 추출 키트
JP2017530366A (ja) クロマトグラフ濃縮を使用する検体の検出方法
JP2022144722A (ja) 標的粒子の分析方法、分析試薬及び分析装置
TWI694146B (zh) 前處理方法及用於其之核酸萃取套組
JP2005533239A (ja) 複雑な基質中の特定の物質を単離および検出する装置および方法
WO2017116695A1 (en) Assembly and method for field filtration of water samples
WO2018013426A2 (en) Methods and devices for performing flow-through capture of low-concentration analytes

Legal Events

Date Code Title Description
A201 Request for examination
PA0105 International application

St.27 status event code: A-0-1-A10-A15-nap-PA0105

PA0201 Request for examination

St.27 status event code: A-1-2-D10-D11-exm-PA0201

PG1501 Laying open of application

St.27 status event code: A-1-1-Q10-Q12-nap-PG1501

PN2301 Change of applicant

St.27 status event code: A-3-3-R10-R13-asn-PN2301

St.27 status event code: A-3-3-R10-R11-asn-PN2301

D13-X000 Search requested

St.27 status event code: A-1-2-D10-D13-srh-X000

D14-X000 Search report completed

St.27 status event code: A-1-2-D10-D14-srh-X000

E902 Notification of reason for refusal
PE0902 Notice of grounds for rejection

St.27 status event code: A-1-2-D10-D21-exm-PE0902

R17-X000 Change to representative recorded

St.27 status event code: A-3-3-R10-R17-oth-X000

T11-X000 Administrative time limit extension requested

St.27 status event code: U-3-3-T10-T11-oth-X000

E13-X000 Pre-grant limitation requested

St.27 status event code: A-2-3-E10-E13-lim-X000

P11-X000 Amendment of application requested

St.27 status event code: A-2-2-P10-P11-nap-X000

P13-X000 Application amended

St.27 status event code: A-2-2-P10-P13-nap-X000

E701 Decision to grant or registration of patent right
PE0701 Decision of registration

St.27 status event code: A-1-2-D10-D22-exm-PE0701

GRNT Written decision to grant
PR0701 Registration of establishment

St.27 status event code: A-2-4-F10-F11-exm-PR0701

PR1002 Payment of registration fee

St.27 status event code: A-2-2-U10-U12-oth-PR1002

Fee payment year number: 1

PG1601 Publication of registration

St.27 status event code: A-4-4-Q10-Q13-nap-PG1601

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 4

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 5

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 6

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 7

PR1001 Payment of annual fee

St.27 status event code: A-4-4-U10-U11-oth-PR1001

Fee payment year number: 8

U11 Full renewal or maintenance fee paid

Free format text: ST27 STATUS EVENT CODE: A-4-4-U10-U11-OTH-PR1001 (AS PROVIDED BY THE NATIONAL OFFICE)

Year of fee payment: 8