CA2940778A1 - Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes - Google Patents

Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes Download PDF

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Publication number
CA2940778A1
CA2940778A1 CA2940778A CA2940778A CA2940778A1 CA 2940778 A1 CA2940778 A1 CA 2940778A1 CA 2940778 A CA2940778 A CA 2940778A CA 2940778 A CA2940778 A CA 2940778A CA 2940778 A1 CA2940778 A1 CA 2940778A1
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Canada
Prior art keywords
alpha
glucan
fructose
fraction
saccharide
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Abandoned
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CA2940778A
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English (en)
French (fr)
Inventor
Kevin D. Nagy
Erwin Columbus HAGO
Jayarama K. Shetty
Susan Marie Hennessey
Robert Dicosimo
Ling Hua
Rodrigo Ramirez
Zhongmei TANG
Zheyong YU
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EIDP Inc
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EI Du Pont de Nemours and Co
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Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of CA2940778A1 publication Critical patent/CA2940778A1/en
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/02Monosaccharides
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07HSUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/1048Glycosyltransferases (2.4)
    • C12N9/1051Hexosyltransferases (2.4.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2428Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/04Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/04Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C12P19/08Dextran
    • CCHEMISTRY; METALLURGY
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    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/12Disaccharides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/16Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/18Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • C12Y204/01005Dextransucrase (2.4.1.5)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y204/00Glycosyltransferases (2.4)
    • C12Y204/01Hexosyltransferases (2.4.1)
    • C12Y204/010241,4-Alpha-glucan 6-alpha-glucosyltransferase (2.4.1.24)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0102Alpha-glucosidase (3.2.1.20)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0107Glucan 1,6-alpha-glucosidase (3.2.1.70)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01084Glucan 1,3-alpha-glucosidase (3.2.1.84), i.e. mutanase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01115Branched-dextran exo-1,2-alpha-glucosidase (3.2.1.115)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2940778A 2014-02-27 2015-02-26 Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes Abandoned CA2940778A1 (en)

Applications Claiming Priority (19)

Application Number Priority Date Filing Date Title
US201461945241P 2014-02-27 2014-02-27
US201461945233P 2014-02-27 2014-02-27
US61/945,241 2014-02-27
US61/945,233 2014-02-27
US201462004312P 2014-05-29 2014-05-29
US201462004314P 2014-05-29 2014-05-29
US201462004290P 2014-05-29 2014-05-29
US201462004305P 2014-05-29 2014-05-29
US201462004300P 2014-05-29 2014-05-29
US201462004308P 2014-05-29 2014-05-29
US62/004,314 2014-05-29
US62/004,308 2014-05-29
US62/004,290 2014-05-29
US62/004,305 2014-05-29
US62/004,300 2014-05-29
US62/004,312 2014-05-29
CNPCT/CN2015/073269 2015-02-25
CN2015073269 2015-02-25
PCT/US2015/017648 WO2015130883A1 (en) 2014-02-27 2015-02-26 Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes

Publications (1)

Publication Number Publication Date
CA2940778A1 true CA2940778A1 (en) 2015-09-03

Family

ID=52633687

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2940778A Abandoned CA2940778A1 (en) 2014-02-27 2015-02-26 Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes
CA2938144A Abandoned CA2938144A1 (en) 2014-02-27 2015-02-26 Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes

Family Applications After (1)

Application Number Title Priority Date Filing Date
CA2938144A Abandoned CA2938144A1 (en) 2014-02-27 2015-02-26 Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes

Country Status (7)

Country Link
EP (2) EP3110959A1 (enExample)
JP (2) JP6929499B2 (enExample)
CN (2) CN106068327A (enExample)
AU (2) AU2015223025B2 (enExample)
BR (1) BR112016019823B1 (enExample)
CA (2) CA2940778A1 (enExample)
WO (2) WO2015130883A1 (enExample)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR102625499B1 (ko) 2014-12-22 2024-01-17 뉴트리션 앤드 바이오사이언시스 유에스에이 4, 인크. 폴리 알파-1,3-글루칸 함유 폴리머 블렌드
EP3253454B1 (en) 2015-02-06 2020-04-22 DuPont Industrial Biosciences USA, LLC Colloidal dispersions of poly alpha-1,3-glucan based polymers
EP3262180A1 (en) 2015-02-25 2018-01-03 Danisco US Inc. Alpha-glucosidase, compositions & methods
EP3303411B1 (en) 2015-06-01 2020-11-18 DuPont Industrial Biosciences USA, LLC Structured liquid compositions comprising colloidal dispersions of poly alpha-1,3-glucan
HRP20191813T1 (hr) 2015-06-01 2019-12-27 E. I. Du Pont De Nemours And Company Fibridi poli alfa-1,3-glukana i njihove uporabe i postupci za pripremu fibrida poli alfa-1,3-glukana
CA2997563C (en) 2015-10-26 2022-03-22 E. I. Du Pont De Nemours And Company Polysaccharide coatings for paper
KR102746152B1 (ko) 2015-10-26 2024-12-26 뉴트리션 앤드 바이오사이언시스 유에스에이 4, 인크. 수불용성 알파-(1,3-글루칸) 조성물
EP3374400B1 (en) * 2015-11-13 2022-04-13 Nutrition & Biosciences USA 4, Inc. Glucan fiber compositions for use in laundry care and fabric care
WO2017083229A1 (en) * 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
EP3374488B1 (en) 2015-11-13 2020-10-14 DuPont Industrial Biosciences USA, LLC Glucan fiber compositions for use in laundry care and fabric care
US10266861B2 (en) * 2015-12-14 2019-04-23 E. I. Du Pont De Nemours And Company Production and composition of fructose syrup
US10895028B2 (en) 2015-12-14 2021-01-19 Dupont Industrial Biosciences Usa, Llc Nonwoven glucan webs
WO2018112376A1 (en) * 2016-12-15 2018-06-21 Danisco Us Inc. Method for increasing the production of ethanol from corn fiber in a starch hydrolysis process
JP2018139554A (ja) * 2017-02-28 2018-09-13 日本食品化工株式会社 水溶性食物繊維含有組成物及びその製造方法
US10774352B2 (en) 2017-05-23 2020-09-15 Dupont Industrial Biosciences Usa, Llc Enzymatic production of alpha-1,3-glucan
EP3415622A1 (en) * 2017-06-14 2018-12-19 Evonik Degussa GmbH Method for production of fine chemicals using a corynebacterium secreting modified alpha-1,6-glucosidases
EP3668970A1 (en) * 2017-09-13 2020-06-24 DuPont Industrial Biosciences USA, LLC Engineered glucosyltransferases
EP3707168B1 (en) * 2017-11-10 2022-03-30 Nutrition & Biosciences USA 4, Inc. Unique morphological polysaccharide
WO2019165060A1 (en) * 2018-02-23 2019-08-29 Danisco Us Inc Synthesis of glucan comprising alpha-1,3 glycosidic linkages with phosphorylase enzymes
CN115197884A (zh) * 2022-08-24 2022-10-18 吉林大学 一种降解废弃淀粉的微生物菌剂及其应用方法
CN120118963A (zh) * 2023-12-08 2025-06-10 宁夏伊品生物科技股份有限公司 aglA基因在制备L-氨基酸中的应用

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WO2013096511A1 (en) * 2011-12-19 2013-06-27 E. I. Du Pont De Nemours And Company Increased poly (alpha - 1, 3 - glucan) yield using tetraborate
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Publication number Publication date
CN106460023A (zh) 2017-02-22
AU2015223027A1 (en) 2016-08-04
AU2015223025A1 (en) 2016-08-04
JP7011393B2 (ja) 2022-02-10
EP3110958A1 (en) 2017-01-04
JP6929499B2 (ja) 2021-09-01
EP3110959A1 (en) 2017-01-04
JP2017518025A (ja) 2017-07-06
AU2015223025B2 (en) 2018-09-20
BR112016019823A2 (pt) 2017-10-17
BR112016019823B1 (pt) 2023-05-09
JP2017518026A (ja) 2017-07-06
CA2938144A1 (en) 2015-09-03
WO2015130881A1 (en) 2015-09-03
CN106068327A (zh) 2016-11-02
WO2015130883A9 (en) 2016-08-18
AU2015223027B2 (en) 2018-09-20
BR112016019820A2 (pt) 2017-10-17
WO2015130883A1 (en) 2015-09-03

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