BR112016019823B1 - Método de síntese de glucano e hidrólise de subproduto de sacarídeo solúvel - Google Patents

Método de síntese de glucano e hidrólise de subproduto de sacarídeo solúvel Download PDF

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Publication number
BR112016019823B1
BR112016019823B1 BR112016019823-9A BR112016019823A BR112016019823B1 BR 112016019823 B1 BR112016019823 B1 BR 112016019823B1 BR 112016019823 A BR112016019823 A BR 112016019823A BR 112016019823 B1 BR112016019823 B1 BR 112016019823B1
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Brazil
Prior art keywords
alpha
glucan
enzyme
fraction
saccharide
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BR112016019823-9A
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English (en)
Portuguese (pt)
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BR112016019823A2 (pt
Inventor
Kevin D Nagy
Erwin Columbus Hago
Jayarama K Shetty
Susan Marie Hennessey
Robert DiCosimo
Ling Hua
Rodrigo Ramirez
Zhongmei Tang
Zheyong Yu
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Nutrition & Biosciences Usa 4, Inc
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Publication of BR112016019823A2 publication Critical patent/BR112016019823A2/pt
Publication of BR112016019823B1 publication Critical patent/BR112016019823B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/16—Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C12P19/08—Dextran
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01005—Dextransucrase (2.4.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01024—1,4-Alpha-glucan 6-alpha-glucosyltransferase (2.4.1.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0102—Alpha-glucosidase (3.2.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0107—Glucan 1,6-alpha-glucosidase (3.2.1.70)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01084—Glucan 1,3-alpha-glucosidase (3.2.1.84), i.e. mutanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01115—Branched-dextran exo-1,2-alpha-glucosidase (3.2.1.115)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
BR112016019823-9A 2014-02-27 2015-02-26 Método de síntese de glucano e hidrólise de subproduto de sacarídeo solúvel BR112016019823B1 (pt)

Applications Claiming Priority (15)

Application Number Priority Date Filing Date Title
US201461945241P 2014-02-27 2014-02-27
US201461945233P 2014-02-27 2014-02-27
US61/945,241 2014-02-27
US61/945,233 2014-02-27
US201462004290P 2014-05-29 2014-05-29
US201462004312P 2014-05-29 2014-05-29
US201462004300P 2014-05-29 2014-05-29
US201462004308P 2014-05-29 2014-05-29
US201462004314P 2014-05-29 2014-05-29
US201462004305P 2014-05-29 2014-05-29
US62/004,308 2014-05-29
US62/004,312 2014-05-29
CNPCT/CN2015/073269 2015-02-25
CN2015073269 2015-02-25
PCT/US2015/017644 WO2015130881A1 (en) 2014-02-27 2015-02-26 Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes

Publications (2)

Publication Number Publication Date
BR112016019823A2 BR112016019823A2 (pt) 2017-10-17
BR112016019823B1 true BR112016019823B1 (pt) 2023-05-09

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BR112016019823-9A BR112016019823B1 (pt) 2014-02-27 2015-02-26 Método de síntese de glucano e hidrólise de subproduto de sacarídeo solúvel

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Country Link
EP (2) EP3110959A1 (enExample)
JP (2) JP6929499B2 (enExample)
CN (2) CN106068327A (enExample)
AU (2) AU2015223027B2 (enExample)
BR (1) BR112016019823B1 (enExample)
CA (2) CA2940778A1 (enExample)
WO (2) WO2015130881A1 (enExample)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2015369929B2 (en) 2014-12-22 2020-08-20 Nutrition & Biosciences USA 4, Inc. Polymeric blend containing poly alpha-1,3-glucan
WO2016126685A1 (en) 2015-02-06 2016-08-11 E. I. Du Pont De Nemours And Company Colloidal dispersions of poly alpha-1,3-glucan based polymers
WO2016138315A1 (en) * 2015-02-25 2016-09-01 Danisco Us Inc Alpha-glucosidase, compositions & methods
CN107995923B (zh) 2015-06-01 2021-11-02 营养与生物科学美国4公司 包含聚α-1,3-葡聚糖的胶体分散体的结构化的液体组合物
JP6956013B2 (ja) 2015-06-01 2021-10-27 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド ポリα−１，３−グルカンフィブリッド及びその使用、並びにポリα−１，３−グルカンフィブリッドを製造する方法
US11230812B2 (en) 2015-10-26 2022-01-25 Nutrition & Biosciences Usa 4, Inc Polysaccharide coatings for paper
US10731297B2 (en) 2015-10-26 2020-08-04 Dupont Industrial Biosciences Usa, Llc Water insoluble alpha-(1,3-glucan) composition
JP6997706B2 (ja) 2015-11-13 2022-01-18 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド 洗濯ケアおよび織物ケアにおいて使用するためのグルカン繊維組成物
WO2017083229A1 (en) * 2015-11-13 2017-05-18 E. I. Du Pont De Nemours And Company Glucan fiber compositions for use in laundry care and fabric care
JP7045313B2 (ja) 2015-11-13 2022-03-31 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド 洗濯ケアおよび織物ケアにおいて使用するためのグルカン繊維組成物
US10895028B2 (en) 2015-12-14 2021-01-19 Dupont Industrial Biosciences Usa, Llc Nonwoven glucan webs
US10266861B2 (en) * 2015-12-14 2019-04-23 E. I. Du Pont De Nemours And Company Production and composition of fructose syrup
CA3047306A1 (en) * 2016-12-15 2018-06-21 Danisco Us Inc. Method for increasing the production of ethanol from corn fiber in a starch hydrolysis process
JP2018139554A (ja) * 2017-02-28 2018-09-13 日本食品化工株式会社 水溶性食物繊維含有組成物及びその製造方法
BR112019020201A2 (pt) 2017-05-23 2020-06-30 E. I. Du Pont De Nemours And Company método de produção de alfa-1,3-glucano e composições
EP3415622A1 (en) * 2017-06-14 2018-12-19 Evonik Degussa GmbH Method for production of fine chemicals using a corynebacterium secreting modified alpha-1,6-glucosidases
AU2018334514B2 (en) * 2017-09-13 2025-01-30 Nutrition & Biosciences USA 4, Inc. Engineered glucosyltransferases
US20200262984A1 (en) * 2017-11-10 2020-08-20 Dupont Industrial Biosciences Usa, Llc Unique morphological polysaccharide
CN112004938A (zh) * 2018-02-23 2020-11-27 丹尼斯科美国公司 用磷酸化酶合成包含α-1,3糖苷键的葡聚糖
CN115197884A (zh) * 2022-08-24 2022-10-18 吉林大学 一种降解废弃淀粉的微生物菌剂及其应用方法
CN120118963A (zh) * 2023-12-08 2025-06-10 宁夏伊品生物科技股份有限公司 aglA基因在制备L-氨基酸中的应用

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1173381A (en) * 1980-03-05 1984-08-28 Peter L. Rogers Ethanol production in a continuous process with cell recycle
FR2626583B1 (fr) * 1988-01-29 1991-03-15 Bioeurope Procede de preparation enzymatique d'oligodextranes utiles dans la fabrication de substituts du sucre, et nouveaux oligodextranes
JPH06133791A (ja) * 1992-10-23 1994-05-17 Fujisawa Pharmaceut Co Ltd 低分子デキストラン
EP1109916A1 (en) * 1998-09-02 2001-06-27 Planttec Biotechnologie GmbH Nucleic acid molecules encoding an amylosucrase
EP1165867B1 (en) * 1999-01-25 2004-04-14 E.I. Du Pont De Nemours And Company Polysaccharide fibers
US6562600B1 (en) * 2001-06-25 2003-05-13 The United States Of America As Represented By The Secretary Of Agriculture Production of cyclic alternan tetrasaccharides from oligosaccharide substrates
JP2005185182A (ja) * 2003-12-25 2005-07-14 Lion Corp α−１，３−グルコオリゴ糖の製造方法、該製造方法により得られるα−１，３−グルコオリゴ糖およびこれを含有する製剤組成物
US20080121227A1 (en) * 2004-09-01 2008-05-29 Novozymes A/S Liquefaction and Saccharification Process
US9127287B2 (en) * 2008-06-11 2015-09-08 Syngenta Participations Ag Compositions and methods for producing fermentable carbohydrates
JP2012523852A (ja) * 2009-04-20 2012-10-11 クテロス， インコーポレイテッド バイオマス発酵のための組成物および方法
WO2010129839A1 (en) * 2009-05-07 2010-11-11 Tate & Lyle Ingredients France SAS Compositions and methods for making alpha-(1,2)-branched alpha-(1,6) oligodextrans
JP2012055307A (ja) * 2010-08-09 2012-03-22 Tottori Univ きのこを用いたバイオマスからの効率的エタノール生産システムの開発
US9080195B2 (en) * 2011-09-09 2015-07-14 E I Du Pont De Nemours And Company High titer production of poly (α 1,3 glucan)
US8642757B2 (en) * 2011-09-09 2014-02-04 E I Du Pont De Nemours And Company High titer production of highly linear poly (α 1,3 glucan)
US8828689B2 (en) * 2011-12-19 2014-09-09 E I Du Pont De Nemours And Company Increased poly (α 1, 3 glucan) yield using boric acid
US8962282B2 (en) * 2011-12-19 2015-02-24 E I Du Pont De Nemours And Company Increased poly (alpha 1,3 glucan) yield using tetraborate

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EP3110958A1 (en) 2017-01-04
JP2017518026A (ja) 2017-07-06
CA2938144A1 (en) 2015-09-03
CN106460023B (zh) 2026-02-24
JP2017518025A (ja) 2017-07-06
JP6929499B2 (ja) 2021-09-01
EP3110959A1 (en) 2017-01-04
AU2015223027A1 (en) 2016-08-04
AU2015223027B2 (en) 2018-09-20
BR112016019823A2 (pt) 2017-10-17
WO2015130881A1 (en) 2015-09-03
CA2940778A1 (en) 2015-09-03
AU2015223025A1 (en) 2016-08-04
JP7011393B2 (ja) 2022-02-10
WO2015130883A9 (en) 2016-08-18
CN106460023A (zh) 2017-02-22
BR112016019820A2 (pt) 2017-10-17
AU2015223025B2 (en) 2018-09-20
WO2015130883A1 (en) 2015-09-03
CN106068327A (zh) 2016-11-02

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