CA2940778A1 - Hydrolyse enzymatique de disaccharides et d'oligosaccharides a l'aide d'enzymes alpha-glucosidase - Google Patents

Hydrolyse enzymatique de disaccharides et d'oligosaccharides a l'aide d'enzymes alpha-glucosidase Download PDF

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Publication number
CA2940778A1
CA2940778A1 CA2940778A CA2940778A CA2940778A1 CA 2940778 A1 CA2940778 A1 CA 2940778A1 CA 2940778 A CA2940778 A CA 2940778A CA 2940778 A CA2940778 A CA 2940778A CA 2940778 A1 CA2940778 A1 CA 2940778A1
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CA
Canada
Prior art keywords
alpha
glucan
fructose
fraction
saccharide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2940778A
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English (en)
Inventor
Kevin D. Nagy
Erwin Columbus HAGO
Jayarama K. Shetty
Susan Marie Hennessey
Robert Dicosimo
Ling Hua
Rodrigo Ramirez
Zhongmei TANG
Zheyong YU
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
EIDP Inc
Original Assignee
EI Du Pont de Nemours and Co
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Filing date
Publication date
Application filed by EI Du Pont de Nemours and Co filed Critical EI Du Pont de Nemours and Co
Publication of CA2940778A1 publication Critical patent/CA2940778A1/fr
Abandoned legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C12P19/08—Dextran
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/16—Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01005—Dextransucrase (2.4.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01024—1,4-Alpha-glucan 6-alpha-glucosyltransferase (2.4.1.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0102—Alpha-glucosidase (3.2.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0107—Glucan 1,6-alpha-glucosidase (3.2.1.70)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01084—Glucan 1,3-alpha-glucosidase (3.2.1.84), i.e. mutanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01115—Branched-dextran exo-1,2-alpha-glucosidase (3.2.1.115)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne un procédé pour l'hydrolysation d'une liaison alpha-1,5 glucosyl-fructose dans un saccharide (disaccharide ou oligosaccharide) comme le leucrose. Ce procédé consiste à mettre en contact le saccharide avec une enzyme alpha-glucosidase, comme de la transglucosidase ou de la glucoamylase, dans des conditions appropriées, étape de contact durant laquelle l'enzyme hydrolyse au moins une liaison alpha-1,5 glucosyl-fructose du saccharide. Ce procédé est utile pour réduire la quantité de leucrose dans un filtrat isolé à partir d'une réaction de synthèse de glucane, par exemple.
CA2940778A 2014-02-27 2015-02-26 Hydrolyse enzymatique de disaccharides et d'oligosaccharides a l'aide d'enzymes alpha-glucosidase Abandoned CA2940778A1 (fr)

Applications Claiming Priority (19)

Application Number Priority Date Filing Date Title
US201461945241P 2014-02-27 2014-02-27
US201461945233P 2014-02-27 2014-02-27
US61/945,233 2014-02-27
US61/945,241 2014-02-27
US201462004314P 2014-05-29 2014-05-29
US201462004308P 2014-05-29 2014-05-29
US201462004290P 2014-05-29 2014-05-29
US201462004312P 2014-05-29 2014-05-29
US201462004300P 2014-05-29 2014-05-29
US201462004305P 2014-05-29 2014-05-29
US62/004,314 2014-05-29
US62/004,305 2014-05-29
US62/004,300 2014-05-29
US62/004,290 2014-05-29
US62/004,308 2014-05-29
US62/004,312 2014-05-29
CN2015073269 2015-02-25
CNPCT/CN2015/073269 2015-02-25
PCT/US2015/017648 WO2015130883A1 (fr) 2014-02-27 2015-02-26 Hydrolyse enzymatique de disaccharides et d'oligosaccharides à l'aide d'enzymes alpha-glucosidase

Publications (1)

Publication Number Publication Date
CA2940778A1 true CA2940778A1 (fr) 2015-09-03

Family

ID=52633687

Family Applications (2)

Application Number Title Priority Date Filing Date
CA2938144A Abandoned CA2938144A1 (fr) 2014-02-27 2015-02-26 Hydrolyse enzymatique de disaccharides et d'oligosaccharides a l'aide d'enzymes alpha-glucosidases
CA2940778A Abandoned CA2940778A1 (fr) 2014-02-27 2015-02-26 Hydrolyse enzymatique de disaccharides et d'oligosaccharides a l'aide d'enzymes alpha-glucosidase

Family Applications Before (1)

Application Number Title Priority Date Filing Date
CA2938144A Abandoned CA2938144A1 (fr) 2014-02-27 2015-02-26 Hydrolyse enzymatique de disaccharides et d'oligosaccharides a l'aide d'enzymes alpha-glucosidases

Country Status (7)

Country Link
EP (2) EP3110958A1 (fr)
JP (2) JP6929499B2 (fr)
CN (2) CN106068327A (fr)
AU (2) AU2015223027B2 (fr)
BR (1) BR112016019823B1 (fr)
CA (2) CA2938144A1 (fr)
WO (2) WO2015130881A1 (fr)

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BR112017013368B1 (pt) 2014-12-22 2021-11-23 Nutrition & Biosciences USA 4, Inc. Composição de mistura polimérica
JP6963502B2 (ja) 2015-02-06 2021-11-10 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド ポリα−１，３−グルカン系ポリマーのコロイド分散液
EP3262180A1 (fr) 2015-02-25 2018-01-03 Danisco US Inc. Alpha-glucosidase, compositions et procédés
EP3303411B1 (fr) 2015-06-01 2020-11-18 DuPont Industrial Biosciences USA, LLC Compositions liquides structurées comprenant une distpersion colloïdale de poly alpha-1,3-glucane
SI3303697T1 (sl) * 2015-06-01 2020-02-28 E.I. Du Pont De Nemours And Company Fibridi poli ALFA-1,3-glukana in njegove uporabe ter postopki za izdelavo fibridov poli ALFA-1,3-glukana
ES2939179T3 (es) 2015-10-26 2023-04-19 Nutrition & Biosciences Usa 4 Inc Recubrimientos de polisacáridos para papel
KR20180074734A (ko) 2015-10-26 2018-07-03 이 아이 듀폰 디 네모아 앤드 캄파니 수불용성 알파-(1,3→글루칸) 조성물
JP2019504932A (ja) 2015-11-13 2019-02-21 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニーＥ．Ｉ．Ｄｕ Ｐｏｎｔ Ｄｅ Ｎｅｍｏｕｒｓ Ａｎｄ Ｃｏｍｐａｎｙ 洗濯ケアおよび織物ケアにおいて使用するためのグルカン繊維組成物
US10876074B2 (en) 2015-11-13 2020-12-29 Dupont Industrial Biosciences Usa, Llc Glucan fiber compositions for use in laundry care and fabric care
JP7045313B2 (ja) 2015-11-13 2022-03-31 ニュートリション・アンド・バイオサイエンシーズ・ユーエスエー・フォー，インコーポレイテッド 洗濯ケアおよび織物ケアにおいて使用するためのグルカン繊維組成物
US10895028B2 (en) 2015-12-14 2021-01-19 Dupont Industrial Biosciences Usa, Llc Nonwoven glucan webs
US10266861B2 (en) * 2015-12-14 2019-04-23 E. I. Du Pont De Nemours And Company Production and composition of fructose syrup
BR112019012291A2 (pt) * 2016-12-15 2019-11-19 Danisco Us Inc método para aumentar a produção de etanol a partir de fibra de milho em um processo de hidrólise de amido
JP2018139554A (ja) * 2017-02-28 2018-09-13 日本食品化工株式会社 水溶性食物繊維含有組成物及びその製造方法
AU2018272823B2 (en) 2017-05-23 2024-05-09 Nutrition & Biosciences USA 4, Inc. Enzymatic production of alpha-1,3-glucan
EP3415622A1 (fr) * 2017-06-14 2018-12-19 Evonik Degussa GmbH Procédé de production de produits chimiques fins au moyen d'une corynebactérie sécrétant des alpha-1,6-glucosidases modifiées
US10865393B2 (en) * 2017-09-13 2020-12-15 Dupont Industrial Biosciences Usa, Llc Engineered glucosyltransferases
CN111566128B (zh) * 2017-11-10 2022-06-21 营养与生物科学美国4公司 独特形态的多糖
EP3740583A1 (fr) * 2018-02-23 2020-11-25 Danisco US Inc. Synthèse de glucane comprenant des liaisons glycosidiques alpha-1,3 avec des enzymes phosphorylase
CN115197884A (zh) * 2022-08-24 2022-10-18 吉林大学 一种降解废弃淀粉的微生物菌剂及其应用方法

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WO2013096502A1 (fr) * 2011-12-19 2013-06-27 E. I. Du Pont De Nemours And Company Augmentation du rendement en poly(1,3-glucane) au moyen d'acide borique
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Also Published As

Publication number Publication date
JP7011393B2 (ja) 2022-02-10
BR112016019820A2 (pt) 2017-10-17
EP3110958A1 (fr) 2017-01-04
JP2017518025A (ja) 2017-07-06
AU2015223025B2 (en) 2018-09-20
CN106460023A (zh) 2017-02-22
CA2938144A1 (fr) 2015-09-03
CN106068327A (zh) 2016-11-02
WO2015130881A1 (fr) 2015-09-03
WO2015130883A1 (fr) 2015-09-03
AU2015223025A1 (en) 2016-08-04
BR112016019823A2 (pt) 2017-10-17
WO2015130883A9 (fr) 2016-08-18
AU2015223027B2 (en) 2018-09-20
AU2015223027A1 (en) 2016-08-04
JP6929499B2 (ja) 2021-09-01
JP2017518026A (ja) 2017-07-06
BR112016019823B1 (pt) 2023-05-09
EP3110959A1 (fr) 2017-01-04

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Date Code Title Description
FZDE Discontinued

Effective date: 20210831

FZDE Discontinued

Effective date: 20210831