AU2015223025B2 - Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes - Google Patents

Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes Download PDF

Info

Publication number
AU2015223025B2
AU2015223025B2 AU2015223025A AU2015223025A AU2015223025B2 AU 2015223025 B2 AU2015223025 B2 AU 2015223025B2 AU 2015223025 A AU2015223025 A AU 2015223025A AU 2015223025 A AU2015223025 A AU 2015223025A AU 2015223025 B2 AU2015223025 B2 AU 2015223025B2
Authority
AU
Australia
Prior art keywords
ala
ser
thr
gly
val
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
AU2015223025A
Other languages
English (en)
Other versions
AU2015223025A1 (en
Inventor
Robert Dicosimo
Erwin Columbus HAGO
Susan Marie Hennessey
Ling Hua
Kevin D. Nagy
Rodrigo Ramirez
Jayarama K. Shetty
Zhongmei TANG
Zheyong YU
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nutrition and Biosciences USA 4 Inc
Original Assignee
Nutrition and Biosciences USA 4 Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nutrition and Biosciences USA 4 Inc filed Critical Nutrition and Biosciences USA 4 Inc
Publication of AU2015223025A1 publication Critical patent/AU2015223025A1/en
Application granted granted Critical
Publication of AU2015223025B2 publication Critical patent/AU2015223025B2/en
Assigned to Nutrition & Biosciences USA 4, Inc. reassignment Nutrition & Biosciences USA 4, Inc. Request for Assignment Assignors: E. I. DU PONT DE NEMOURS AND COMPANY
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/16—Preparation of compounds containing saccharide radicals produced by the action of an alpha-1, 6-glucosidase, e.g. amylose, debranched amylopectin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • C12P19/08—Dextran
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/12—Disaccharides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01005—Dextransucrase (2.4.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01024—1,4-Alpha-glucan 6-alpha-glucosyltransferase (2.4.1.24)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01003—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0102—Alpha-glucosidase (3.2.1.20)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/0107—Glucan 1,6-alpha-glucosidase (3.2.1.70)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01084—Glucan 1,3-alpha-glucosidase (3.2.1.84), i.e. mutanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01115—Branched-dextran exo-1,2-alpha-glucosidase (3.2.1.115)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AU2015223025A 2014-02-27 2015-02-26 Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes Active AU2015223025B2 (en)

Applications Claiming Priority (19)

Application Number Priority Date Filing Date Title
US201461945233P 2014-02-27 2014-02-27
US201461945241P 2014-02-27 2014-02-27
US61/945,241 2014-02-27
US61/945,233 2014-02-27
US201462004314P 2014-05-29 2014-05-29
US201462004312P 2014-05-29 2014-05-29
US201462004290P 2014-05-29 2014-05-29
US201462004300P 2014-05-29 2014-05-29
US201462004308P 2014-05-29 2014-05-29
US201462004305P 2014-05-29 2014-05-29
US62/004,314 2014-05-29
US62/004,290 2014-05-29
US62/004,312 2014-05-29
US62/004,300 2014-05-29
US62/004,308 2014-05-29
US62/004,305 2014-05-29
CNPCT/CN2015/073269 2015-02-25
CN2015073269 2015-02-25
PCT/US2015/017644 WO2015130881A1 (en) 2014-02-27 2015-02-26 Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes

Publications (2)

Publication Number Publication Date
AU2015223025A1 AU2015223025A1 (en) 2016-08-04
AU2015223025B2 true AU2015223025B2 (en) 2018-09-20

Family

ID=52633687

Family Applications (2)

Application Number Title Priority Date Filing Date
AU2015223025A Active AU2015223025B2 (en) 2014-02-27 2015-02-26 Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes
AU2015223027A Active AU2015223027B2 (en) 2014-02-27 2015-02-26 Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes

Family Applications After (1)

Application Number Title Priority Date Filing Date
AU2015223027A Active AU2015223027B2 (en) 2014-02-27 2015-02-26 Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes

Country Status (7)

Country Link
EP (2) EP3110958A1 (enExample)
JP (2) JP6929499B2 (enExample)
CN (2) CN106068327A (enExample)
AU (2) AU2015223025B2 (enExample)
BR (1) BR112016019823B1 (enExample)
CA (2) CA2940778A1 (enExample)
WO (2) WO2015130881A1 (enExample)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP6785242B2 (ja) 2014-12-22 2020-11-18 デュポン・インダストリアル・バイオサイエンシーズ・ユーエスエイ・エルエルシー ポリアルファ−１，３−グルカンを含有するポリマーブレンド
BR112017012481B1 (pt) 2015-02-06 2021-10-19 Nutrition & Biosciences USA 4, Inc. Dispersão coloidal, produto de cuidados pessoais, produto alimentício e processo de fabricação de uma dispersão coloidal
EP3262180A1 (en) * 2015-02-25 2018-01-03 Danisco US Inc. Alpha-glucosidase, compositions & methods
WO2016196022A1 (en) * 2015-06-01 2016-12-08 E I Du Pont De Nemours And Company Poly alpha-1,3-glucan fibrids and uses thereof and processes to make poly alpha-1,3-glucan fibrids
CN107995923B (zh) 2015-06-01 2021-11-02 营养与生物科学美国4公司 包含聚α-1,3-葡聚糖的胶体分散体的结构化的液体组合物
ES2937288T3 (es) 2015-10-26 2023-03-27 Nutrition & Biosciences Usa 4 Inc Composición de alfa-(1,3->glucano) insoluble en agua
CA2997563C (en) 2015-10-26 2022-03-22 E. I. Du Pont De Nemours And Company Polysaccharide coatings for paper
US10876074B2 (en) 2015-11-13 2020-12-29 Dupont Industrial Biosciences Usa, Llc Glucan fiber compositions for use in laundry care and fabric care
US10844324B2 (en) 2015-11-13 2020-11-24 Dupont Industrial Biosciences Usa, Llc Glucan fiber compositions for use in laundry care and fabric care
EP3374401B1 (en) * 2015-11-13 2022-04-06 Nutrition & Biosciences USA 4, Inc. Glucan fiber compositions for use in laundry care and fabric care
US10266861B2 (en) * 2015-12-14 2019-04-23 E. I. Du Pont De Nemours And Company Production and composition of fructose syrup
US10895028B2 (en) 2015-12-14 2021-01-19 Dupont Industrial Biosciences Usa, Llc Nonwoven glucan webs
WO2018112376A1 (en) * 2016-12-15 2018-06-21 Danisco Us Inc. Method for increasing the production of ethanol from corn fiber in a starch hydrolysis process
JP2018139554A (ja) * 2017-02-28 2018-09-13 日本食品化工株式会社 水溶性食物繊維含有組成物及びその製造方法
AU2018272823B2 (en) 2017-05-23 2024-05-09 Nutrition & Biosciences USA 4, Inc. Enzymatic production of alpha-1,3-glucan
EP3415622A1 (en) * 2017-06-14 2018-12-19 Evonik Degussa GmbH Method for production of fine chemicals using a corynebacterium secreting modified alpha-1,6-glucosidases
EP3668970A1 (en) * 2017-09-13 2020-06-24 DuPont Industrial Biosciences USA, LLC Engineered glucosyltransferases
AU2018365877A1 (en) * 2017-11-10 2020-05-28 Nutrition & Biosciences USA 4, Inc. Unique morphological polysaccharide
JP2021513862A (ja) * 2018-02-23 2021-06-03 ダニスコ・ユーエス・インク ホスホリラーゼ酵素による、アルファ−１，３グリコシド結合を含むグルカンの合成
CN115197884A (zh) * 2022-08-24 2022-10-18 吉林大学 一种降解废弃淀粉的微生物菌剂及其应用方法
CN120118963A (zh) * 2023-12-08 2025-06-10 宁夏伊品生物科技股份有限公司 aglA基因在制备L-氨基酸中的应用

Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5141858A (en) * 1988-01-29 1992-08-25 Bioeurope Method for the production of α(1→2) oligodextrans using Leuconostoc mesenteroides B-1299

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1174191A (en) * 1980-03-05 1984-09-11 Peter L. Rogers Ethanol production
JPH06133791A (ja) * 1992-10-23 1994-05-17 Fujisawa Pharmaceut Co Ltd 低分子デキストラン
WO2000014249A1 (en) * 1998-09-02 2000-03-16 Planttec Biotechnologie Gmbh Nucleic acid molecules encoding an amylosucrase
US7000000B1 (en) * 1999-01-25 2006-02-14 E. I. Du Pont De Nemours And Company Polysaccharide fibers
US6562600B1 (en) * 2001-06-25 2003-05-13 The United States Of America As Represented By The Secretary Of Agriculture Production of cyclic alternan tetrasaccharides from oligosaccharide substrates
JP2005185182A (ja) * 2003-12-25 2005-07-14 Lion Corp α−１，３−グルコオリゴ糖の製造方法、該製造方法により得られるα−１，３−グルコオリゴ糖およびこれを含有する製剤組成物
US20080121227A1 (en) * 2004-09-01 2008-05-29 Novozymes A/S Liquefaction and Saccharification Process
AR072105A1 (es) * 2008-06-11 2010-08-04 Syngenta Participations Ag Composiciones y metodos para la produccion de carbohidratos fermenta-bles en plantas
CA2759726A1 (en) * 2009-04-20 2010-10-28 Qteros, Inc. Compositions and methods for fermentation of biomass
KR101783383B1 (ko) * 2009-05-07 2017-09-29 테이트 ＆ 라일 인그레디언츠 프랑스 에스에이에스 알파-(1,2)-분지형 알파-(1,6) 올리고덱스트란을 제조하기 위한 조성물 및 방법
JP2012055307A (ja) * 2010-08-09 2012-03-22 Tottori Univ きのこを用いたバイオマスからの効率的エタノール生産システムの開発
US8642757B2 (en) * 2011-09-09 2014-02-04 E I Du Pont De Nemours And Company High titer production of highly linear poly (α 1,3 glucan)
US9080195B2 (en) * 2011-09-09 2015-07-14 E I Du Pont De Nemours And Company High titer production of poly (α 1,3 glucan)
WO2013096511A1 (en) * 2011-12-19 2013-06-27 E. I. Du Pont De Nemours And Company Increased poly (alpha - 1, 3 - glucan) yield using tetraborate
US8828689B2 (en) * 2011-12-19 2014-09-09 E I Du Pont De Nemours And Company Increased poly (α 1, 3 glucan) yield using boric acid

Patent Citations (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5141858A (en) * 1988-01-29 1992-08-25 Bioeurope Method for the production of α(1→2) oligodextrans using Leuconostoc mesenteroides B-1299

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
K. POKUSAEVA ET AL, "Characterization of Two Novel -Glucosidases from Bifidobacterium breve UCC2003", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, (2008-12-29), vol. 75, no. 4, doi:10.1128/AEM.02391-08, ISSN 0099-2240, pages 1135 - 1143 *
VAN DEN BROEK L A M ET AL, "Cloning and characterization of two alpha-glucosidases from Bifidobacterium adolescentis DSM20083.", APPLIED MICROBIOLOGY AND BIOTECHNOLOGY MAR 2003, (2003-03), vol. 61, no. 1, ISSN 0175-7598, pages 55 - 60 *

Also Published As

Publication number Publication date
JP2017518026A (ja) 2017-07-06
JP6929499B2 (ja) 2021-09-01
EP3110958A1 (en) 2017-01-04
AU2015223027A1 (en) 2016-08-04
CA2940778A1 (en) 2015-09-03
WO2015130881A1 (en) 2015-09-03
WO2015130883A1 (en) 2015-09-03
WO2015130883A9 (en) 2016-08-18
JP2017518025A (ja) 2017-07-06
CN106068327A (zh) 2016-11-02
BR112016019823B1 (pt) 2023-05-09
AU2015223025A1 (en) 2016-08-04
CA2938144A1 (en) 2015-09-03
AU2015223027B2 (en) 2018-09-20
EP3110959A1 (en) 2017-01-04
BR112016019820A2 (pt) 2017-10-17
JP7011393B2 (ja) 2022-02-10
CN106460023A (zh) 2017-02-22
BR112016019823A2 (pt) 2017-10-17

Similar Documents

Publication Publication Date Title
AU2015223025B2 (en) Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes
US10472657B2 (en) Enzymatic hydrolysis of disaccharides and oligosaccharides using alpha-glucosidase enzymes
EP2421965B1 (en) Carbohydrate degrading polypeptide and uses thereof
EP3890499A1 (en) Use of lactase to provide high gos fiber level and low lactose level
CN112601459A (zh) 葡糖基转移酶在发酵奶基产品中提供改善的质地的用途
JP2015500041A (ja) 混合培養物から調製された酵素カクテル
US9751917B2 (en) Polynucleotide for cell surface layer expression
Singh et al. Microbial inulinases and pullulanases in the food industry
Naidoo et al. Purification and Characterization of an Endoinulinase from Xanthomonas campestris pv. phaseoli KM 24 Mutant
WO2015021601A1 (en) Simultanenous liquifaction and malto-saccharification
WO2013096244A1 (en) Endoglucanase 1b (eg1b) variants
RU2646136C2 (ru) Рекомбинантный штамм мицелиального гриба penicillium verruculosum ( варианты) и способ получения ферментного препарата с его использованием (варианты)
Melkumyan et al. Peculiarity of exoinulinase of a novel producer of citric acid from inulin
BR112016019820B1 (pt) Método de síntese de glucano e hidrólise de subproduto de leucrose
Jegal et al. Continuous biocatalytic production of trehalose in a fixed-bed bioreactor using immobilized trehalose synthase from Pseudomonas stutzeri
CN117821348A (zh) 一种胞外分泌表达κ-卡拉胶酶的重组枯草芽孢杆菌
RU2605635C1 (ru) СПОСОБ ПОЛУЧЕНИЯ ФЕРМЕНТНОГО ПРЕПАРАТА С ЭНДОИНУЛИНАЗНОЙ И САХАРАЗНОЙ АКТИВНОСТЬЮ ПУТЕМ КУЛЬТИВИРОВАНИЯ РЕКОМБИНАНТНОГО ШТАММА МИЦЕЛИАЛЬНОГО ГРИБА PENICILLIUM CANESCENS Sopp INUA3
WO2015072413A1 (ja) キシラン含有組成物の製造方法、及びグルカン含有組成物の製造方法
EP3990655A1 (en) Enzymatic production of levan-based, prebiotic fructooligosaccharides
Kameshnee Naidoo et al. Purification and characterization of an endoinulinase from Xanthomonas campestris pv. phaseoli KM 24 mutant.

Legal Events

Date Code Title Description
FGA Letters patent sealed or granted (standard patent)
PC Assignment registered

Owner name: NUTRITION & BIOSCIENCES USA 4, INC.

Free format text: FORMER OWNER(S): E. I. DU PONT DE NEMOURS AND COMPANY