CA2937611C - Modulation of cell growth and glycosylation in recombinant glycoprotein production - Google Patents

Modulation of cell growth and glycosylation in recombinant glycoprotein production Download PDF

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Publication number
CA2937611C
CA2937611C CA2937611A CA2937611A CA2937611C CA 2937611 C CA2937611 C CA 2937611C CA 2937611 A CA2937611 A CA 2937611A CA 2937611 A CA2937611 A CA 2937611A CA 2937611 C CA2937611 C CA 2937611C
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Canada
Prior art keywords
zinc
manganese
copper
iron
concentrations
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CA2937611A
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English (en)
French (fr)
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CA2937611A1 (en
Inventor
Oliver Popp
Nicola Beaucamp
Georg DRABNER
Stephanie ESSLINGER
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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Application filed by F Hoffmann La Roche AG filed Critical F Hoffmann La Roche AG
Publication of CA2937611A1 publication Critical patent/CA2937611A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Immunology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
CA2937611A 2014-02-27 2015-02-24 Modulation of cell growth and glycosylation in recombinant glycoprotein production Active CA2937611C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP14157030.9 2014-02-27
EP14157030 2014-02-27
PCT/EP2015/053804 WO2015128314A1 (en) 2014-02-27 2015-02-24 Modulation of cell growth and glycosylation in recombinant glycoprotein production

Publications (2)

Publication Number Publication Date
CA2937611A1 CA2937611A1 (en) 2015-09-03
CA2937611C true CA2937611C (en) 2023-01-03

Family

ID=50184787

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2937611A Active CA2937611C (en) 2014-02-27 2015-02-24 Modulation of cell growth and glycosylation in recombinant glycoprotein production

Country Status (20)

Country Link
US (3) US20170058309A1 (https=)
EP (1) EP3110961B1 (https=)
JP (2) JP6831702B2 (https=)
KR (1) KR102280638B1 (https=)
CN (1) CN106133146B (https=)
BR (1) BR112016017660B1 (https=)
CA (1) CA2937611C (https=)
DK (1) DK3110961T3 (https=)
ES (1) ES2769003T3 (https=)
HR (1) HRP20200100T1 (https=)
HU (1) HUE047575T2 (https=)
LT (1) LT3110961T (https=)
MX (1) MX369395B (https=)
PL (1) PL3110961T3 (https=)
PT (1) PT3110961T (https=)
RS (1) RS59881B1 (https=)
RU (1) RU2712562C2 (https=)
SG (1) SG11201606856SA (https=)
SI (1) SI3110961T1 (https=)
WO (1) WO2015128314A1 (https=)

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AU2012340849B2 (en) 2011-11-21 2017-11-02 Innovation Hammer Llc Methods and systems for growing plants using silicate-based substrates, cultivation of enhanced photosynthetic productivity and photosafening by utilization of exogenous glycopyranosides for endogenous glycopyranosyl-protein derivatives, and formulations, processes and systems for the same
BR112016017660B1 (pt) * 2014-02-27 2022-04-19 F. Hoffmann-La Roche Ag Método para a produção de uma glicoproteína recombinante
CA2969225C (en) 2014-12-01 2023-08-22 Amgen Inc. Process for manipulating the level of glycan content of a glycoprotein
BR112018072235B1 (pt) 2016-04-29 2023-01-17 Innovation Hammer Llc Método para intensificar o crescimento respiratório de um organismo fotossintético que compreende aplicar uma formulação de compósito de glicano
CA3056011A1 (en) 2017-03-14 2018-09-20 Amgen Inc. Control of total afucosylated glycoforms of antibodies produced in cell culture
WO2019077628A1 (en) * 2017-10-16 2019-04-25 Council Of Scientific & Industrial Research ZINC SUPPLEMENTATION TO DECREASE GALACTOSYLATION OF RECOMBINANT GLYCOPROTEINS
MX2020009605A (es) * 2018-03-16 2021-01-08 Genzyme Corp Metodos para mejorar la viabilidad celular en un biorreactor de produccion.
US12325737B2 (en) * 2018-03-26 2025-06-10 Amgen Inc. Total afucosylated glycoforms of antibodies produced in cell culture
AU2019274782A1 (en) * 2018-05-24 2020-12-03 Ares Trading S.A. Method for controlling the afucosylation level of a glycoprotein composition
HU231514B1 (hu) 2018-11-07 2024-07-28 Richter Gedeon Nyrt. Sejttenyészetben előállított rekombináns glikoprotein glikozilációs mintázatának megváltoztatására szolgáló módszer
EA202191352A1 (ru) * 2018-11-13 2021-08-11 Янссен Байотек, Инк. Контроль содержания микроэлементов-металлов во время продукции антител к cd38
JP2020188737A (ja) * 2019-05-23 2020-11-26 東ソー株式会社 抗体依存性細胞傷害活性が向上した抗体の製造方法
US20230265204A1 (en) * 2020-04-24 2023-08-24 Hoffmann-La Roche Inc. Enzyme and pathway modulation with sulfhydryl compounds and their derivatives
CN121712803A (zh) * 2023-08-16 2026-03-20 葛兰素史密斯克莱知识产权有限公司 控制抗体糖基化谱的方法

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US4767704A (en) 1983-10-07 1988-08-30 Columbia University In The City Of New York Protein-free culture medium
IL87737A (en) 1987-09-11 1993-08-18 Genentech Inc Method for culturing polypeptide factor dependent vertebrate recombinant cells
US5705364A (en) * 1995-06-06 1998-01-06 Genentech, Inc. Mammalian cell culture process
US6030945A (en) 1996-01-09 2000-02-29 Genentech, Inc. Apo-2 ligand
AU4330597A (en) 1996-08-30 1998-03-19 Life Technologies, Inc. Serum-free mammalian cell culture medium, and uses thereof
DE69929198T2 (de) * 1998-05-29 2006-08-10 Genentech, Inc., South San Francisco Zellkulturverfahren für die produktion von glycoproteinen
JP2001120262A (ja) * 1999-10-26 2001-05-08 Welfide Corp 生理活性物質の産生増強方法
EP1360314B1 (en) 2001-02-15 2009-01-14 Centocor, Inc. Chemically defined medium for cultured mammalian cells
US20050287666A1 (en) * 2004-06-29 2005-12-29 Invitrogen Corporation Cell culture medium comprising transition metals or trace elements
TWI384069B (zh) 2004-08-27 2013-02-01 Pfizer Ireland Pharmaceuticals 多胜肽之製法
ES2361559T3 (es) 2004-11-02 2011-06-20 Ares Trading S.A. Medio de cultivo de células exento de suero para mamíferos.
US20070231895A1 (en) 2005-11-02 2007-10-04 Lee Gene W Methods for adapting mammalian cells
EP2495308A1 (en) * 2005-12-08 2012-09-05 Amgen Inc. Improved production of glycoproteins using manganese
EP3255141B1 (en) * 2006-07-13 2021-12-01 Wyeth LLC Production of antibodies with improved glycosylation pattern
ES2541546T3 (es) 2006-11-03 2015-07-21 Wyeth Llc Sustancias que inhiben la glucólisis en cultivo celular
AU2008223133A1 (en) 2007-03-02 2008-09-12 Wyeth Use of copper and glutamate in cell culture for production of polypeptides
US20110053223A1 (en) * 2009-08-14 2011-03-03 Robert Bayer Cell culture methods to make antibodies with enhanced adcc function
EP2531592A1 (en) * 2010-02-04 2012-12-12 Vivalis Fed-batch process using concentrated cell culture medium for the efficient production of biologics in eb66 cells
PL2563906T3 (pl) 2010-04-26 2018-04-30 Novartis Ag Sposób hodowli komórek cho
US9062106B2 (en) 2011-04-27 2015-06-23 Abbvie Inc. Methods for controlling the galactosylation profile of recombinantly-expressed proteins
CN105189735B (zh) 2011-04-29 2019-04-12 拜康研究有限公司 在培养期间减少乳酸累积的方法和生产多肽的方法
EP2809773B1 (en) * 2012-01-30 2020-09-02 Dr. Reddy's Laboratories Limited Process of modulating man5 and/or afucosylation content of glycoprotein composition
US9598667B2 (en) 2013-10-04 2017-03-21 Abbvie Inc. Use of metal ions for modulation of protein glycosylation profiles of recombinant proteins
BR112016017660B1 (pt) * 2014-02-27 2022-04-19 F. Hoffmann-La Roche Ag Método para a produção de uma glicoproteína recombinante

Also Published As

Publication number Publication date
SG11201606856SA (en) 2016-09-29
LT3110961T (lt) 2020-02-10
CN106133146B (zh) 2021-05-04
MX2016010236A (es) 2016-10-13
HUE047575T2 (hu) 2020-04-28
RS59881B1 (sr) 2020-03-31
PT3110961T (pt) 2020-01-29
RU2016138176A3 (https=) 2018-10-29
KR20160125514A (ko) 2016-10-31
WO2015128314A1 (en) 2015-09-03
MX369395B (es) 2019-11-07
PL3110961T3 (pl) 2020-04-30
JP2020054351A (ja) 2020-04-09
US20210222220A1 (en) 2021-07-22
US20240327890A1 (en) 2024-10-03
RU2712562C2 (ru) 2020-01-29
RU2016138176A (ru) 2018-03-29
US20170058309A1 (en) 2017-03-02
ES2769003T3 (es) 2020-06-24
JP2017506515A (ja) 2017-03-09
BR112016017660B1 (pt) 2022-04-19
DK3110961T3 (da) 2020-02-03
HRP20200100T1 (hr) 2020-04-03
CN106133146A (zh) 2016-11-16
SI3110961T1 (sl) 2020-03-31
JP6934507B2 (ja) 2021-09-15
CA2937611A1 (en) 2015-09-03
KR102280638B1 (ko) 2021-07-22
BR112016017660A2 (https=) 2017-08-08
EP3110961B1 (en) 2019-11-27
JP6831702B2 (ja) 2021-02-17
EP3110961A1 (en) 2017-01-04

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