CA2875919C - Method for detecting and identifying enterohemorrhagic escherichia coli. - Google Patents

Method for detecting and identifying enterohemorrhagic escherichia coli. Download PDF

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Publication number
CA2875919C
CA2875919C CA2875919A CA2875919A CA2875919C CA 2875919 C CA2875919 C CA 2875919C CA 2875919 A CA2875919 A CA 2875919A CA 2875919 A CA2875919 A CA 2875919A CA 2875919 C CA2875919 C CA 2875919C
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ehec
primers
seq
crispr
espk
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French (fr)
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CA2875919A1 (en
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Patrick Fach
Sabine Delannoy
Lothar Beutin
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BUNDESINSTITUT fur RISIKOBEWERTUNG
Agence National de Securite Sanitaire de l'Alimentation de l'Environnement Et du Travail
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BUNDESINSTITUT fur RISIKOBEWERTUNG
Agence National de Securite Sanitaire de l'Alimentation de l'Environnement Et du Travail
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2875919A 2012-06-14 2013-06-14 Method for detecting and identifying enterohemorrhagic escherichia coli. Active CA2875919C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP12171941.3 2012-06-14
EP12171941.3A EP2674501A1 (en) 2012-06-14 2012-06-14 Method for detecting and identifying enterohemorrhagic Escherichia coli
PCT/IB2013/054888 WO2013186754A2 (en) 2012-06-14 2013-06-14 Method for detecting and identifying enterohemorrhagic escherichia coli

Publications (2)

Publication Number Publication Date
CA2875919A1 CA2875919A1 (en) 2013-12-19
CA2875919C true CA2875919C (en) 2021-08-31

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CA2875919A Active CA2875919C (en) 2012-06-14 2013-06-14 Method for detecting and identifying enterohemorrhagic escherichia coli.

Country Status (12)

Country Link
US (1) US10190175B2 (enExample)
EP (2) EP2674501A1 (enExample)
JP (1) JP6166365B2 (enExample)
KR (1) KR102065053B1 (enExample)
CN (1) CN104619860B (enExample)
AU (1) AU2013276084B2 (enExample)
BR (1) BR112014031370B1 (enExample)
CA (1) CA2875919C (enExample)
CL (1) CL2014003377A1 (enExample)
ES (1) ES2659469T3 (enExample)
MX (1) MX357697B (enExample)
WO (1) WO2013186754A2 (enExample)

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US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
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US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
CN111218447B (zh) 2013-11-07 2024-10-11 爱迪塔斯医药有限公司 使用统治型gRNA的CRISPR相关方法和组合物
US20150166984A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting alpha-antitrypsin point mutations
AU2015298571B2 (en) 2014-07-30 2020-09-03 President And Fellows Of Harvard College Cas9 proteins including ligand-dependent inteins
WO2016087621A1 (en) * 2014-12-03 2016-06-09 Qiagen Gmbh Highly specific and sensitive methods for detecting enterohemorrhagic escherichia coli serotypes o157:h7 and/or o145:h28
WO2016115441A1 (en) * 2015-01-16 2016-07-21 Kansas State University Research Foundation Co-detection and association of multiple genes from the same genome in a sample
WO2017040348A1 (en) 2015-08-28 2017-03-09 The General Hospital Corporation Engineered crispr-cas9 nucleases
US9512446B1 (en) 2015-08-28 2016-12-06 The General Hospital Corporation Engineered CRISPR-Cas9 nucleases
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CN105821124A (zh) * 2016-04-06 2016-08-03 山东省滨州畜牧兽医研究院 一种大肠杆菌 o26、o45、o121血清型三重pcr检测试剂盒及其引物组
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AU2017308889B2 (en) 2016-08-09 2023-11-09 President And Fellows Of Harvard College Programmable Cas9-recombinase fusion proteins and uses thereof
WO2018039438A1 (en) 2016-08-24 2018-03-01 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
KR20240007715A (ko) 2016-10-14 2024-01-16 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
KR20190123328A (ko) 2017-03-09 2019-10-31 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 암 백신
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Also Published As

Publication number Publication date
EP2674501A1 (en) 2013-12-18
WO2013186754A3 (en) 2014-02-20
US20150176064A1 (en) 2015-06-25
BR112014031370B1 (pt) 2022-06-07
AU2013276084A1 (en) 2015-01-29
EP2861762B1 (en) 2017-12-13
JP6166365B2 (ja) 2017-07-19
KR20150030783A (ko) 2015-03-20
ES2659469T3 (es) 2018-03-15
AU2013276084A8 (en) 2015-02-12
BR112014031370A2 (pt) 2017-07-25
CA2875919A1 (en) 2013-12-19
KR102065053B1 (ko) 2020-01-10
CL2014003377A1 (es) 2015-08-07
MX2014015130A (es) 2015-08-06
WO2013186754A2 (en) 2013-12-19
CN104619860B (zh) 2018-01-30
AU2013276084B2 (en) 2019-04-04
EP2861762A2 (en) 2015-04-22
MX357697B (es) 2018-07-19
JP2015519075A (ja) 2015-07-09
CN104619860A (zh) 2015-05-13
US10190175B2 (en) 2019-01-29

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