CA2875919C - Method for detecting and identifying enterohemorrhagic escherichia coli. - Google Patents
Method for detecting and identifying enterohemorrhagic escherichia coli. Download PDFInfo
- Publication number
- CA2875919C CA2875919C CA2875919A CA2875919A CA2875919C CA 2875919 C CA2875919 C CA 2875919C CA 2875919 A CA2875919 A CA 2875919A CA 2875919 A CA2875919 A CA 2875919A CA 2875919 C CA2875919 C CA 2875919C
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- ehec
- primers
- seq
- crispr
- espk
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12171941.3A EP2674501A1 (en) | 2012-06-14 | 2012-06-14 | Method for detecting and identifying enterohemorrhagic Escherichia coli |
| EP12171941.3 | 2012-06-14 | ||
| PCT/IB2013/054888 WO2013186754A2 (en) | 2012-06-14 | 2013-06-14 | Method for detecting and identifying enterohemorrhagic escherichia coli |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2875919A1 CA2875919A1 (en) | 2013-12-19 |
| CA2875919C true CA2875919C (en) | 2021-08-31 |
Family
ID=48949190
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2875919A Active CA2875919C (en) | 2012-06-14 | 2013-06-14 | Method for detecting and identifying enterohemorrhagic escherichia coli. |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US10190175B2 (enExample) |
| EP (2) | EP2674501A1 (enExample) |
| JP (1) | JP6166365B2 (enExample) |
| KR (1) | KR102065053B1 (enExample) |
| CN (1) | CN104619860B (enExample) |
| AU (1) | AU2013276084B2 (enExample) |
| BR (1) | BR112014031370B1 (enExample) |
| CA (1) | CA2875919C (enExample) |
| CL (1) | CL2014003377A1 (enExample) |
| ES (1) | ES2659469T3 (enExample) |
| MX (1) | MX357697B (enExample) |
| WO (1) | WO2013186754A2 (enExample) |
Families Citing this family (57)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013066438A2 (en) | 2011-07-22 | 2013-05-10 | President And Fellows Of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| LT3401400T (lt) | 2012-05-25 | 2019-06-10 | The Regents Of The University Of California | Būdai ir kompozicijos, skirtos rnr molekulės nukreipiamai tikslinės dnr modifikacijai ir rnr molekulės nukreipiamam transkripcijos moduliavimui |
| EP3135765A1 (en) | 2012-12-06 | 2017-03-01 | Sigma-Aldrich Co. LLC | Crispr-based genome modification and regulation |
| SG11201507378UA (en) | 2013-03-14 | 2015-10-29 | Caribou Biosciences Inc | Compositions and methods of nucleic acid-targeting nucleic acids |
| US10378027B2 (en) | 2013-03-15 | 2019-08-13 | The General Hospital Corporation | RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
| US10760064B2 (en) | 2013-03-15 | 2020-09-01 | The General Hospital Corporation | RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
| WO2014204578A1 (en) | 2013-06-21 | 2014-12-24 | The General Hospital Corporation | Using rna-guided foki nucleases (rfns) to increase specificity for rna-guided genome editing |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9340799B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | MRNA-sensing switchable gRNAs |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| CN106459995B (zh) | 2013-11-07 | 2020-02-21 | 爱迪塔斯医药有限公司 | 使用统治型gRNA的CRISPR相关方法和组合物 |
| US20150166984A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting alpha-antitrypsin point mutations |
| WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| US10920285B2 (en) | 2014-12-03 | 2021-02-16 | Qiagen Gmbh | Highly specific and sensitive methods for detecting enterohemorrhagic Escherichia coli serotypes O157:H7 and/or O145:H28 |
| US10233505B2 (en) | 2015-01-16 | 2019-03-19 | Kansas State University Research Foundation | Co-detection and association of multiple genes from the same genome in a sample |
| US9512446B1 (en) | 2015-08-28 | 2016-12-06 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| AU2016316845B2 (en) | 2015-08-28 | 2022-03-10 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| US9926546B2 (en) | 2015-08-28 | 2018-03-27 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| SG10202104041PA (en) | 2015-10-23 | 2021-06-29 | Harvard College | Nucleobase editors and uses thereof |
| CN105779584A (zh) * | 2015-12-18 | 2016-07-20 | 珠海出入境检验检疫局检验检疫技术中心 | 大肠杆菌o111检测用的引物和探针组、试剂盒及pcr检测方法 |
| CN105821124A (zh) * | 2016-04-06 | 2016-08-03 | 山东省滨州畜牧兽医研究院 | 一种大肠杆菌 o26、o45、o121血清型三重pcr检测试剂盒及其引物组 |
| KR20250103795A (ko) | 2016-08-03 | 2025-07-07 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| US11661590B2 (en) | 2016-08-09 | 2023-05-30 | President And Fellows Of Harvard College | Programmable CAS9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| SG11201903089RA (en) | 2016-10-14 | 2019-05-30 | Harvard College | Aav delivery of nucleobase editors |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| EP3592381A1 (en) | 2017-03-09 | 2020-01-15 | President and Fellows of Harvard College | Cancer vaccine |
| US11898179B2 (en) | 2017-03-09 | 2024-02-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| WO2018165629A1 (en) | 2017-03-10 | 2018-09-13 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| WO2018176009A1 (en) | 2017-03-23 | 2018-09-27 | President And Fellows Of Harvard College | Nucleobase editors comprising nucleic acid programmable dna binding proteins |
| ES2992912T3 (en) | 2017-04-21 | 2024-12-19 | Massachusetts Gen Hospital | Variants of cpf1 (cas12a) with altered pam specificity |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| AU2018273986A1 (en) | 2017-05-25 | 2019-12-12 | The General Hospital Corporation | Bipartite base editor (BBE) architectures and type-II-C-Cas9 zinc finger editing |
| CN111801345A (zh) | 2017-07-28 | 2020-10-20 | 哈佛大学的校长及成员们 | 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物 |
| EP3676376B1 (en) | 2017-08-30 | 2025-01-15 | President and Fellows of Harvard College | High efficiency base editors comprising gam |
| US11795443B2 (en) | 2017-10-16 | 2023-10-24 | The Broad Institute, Inc. | Uses of adenosine base editors |
| WO2019118949A1 (en) | 2017-12-15 | 2019-06-20 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| US10101328B1 (en) | 2017-12-29 | 2018-10-16 | Clear Labs, Inc. | Detection of microorganisms in food samples and food processing facilities |
| SG11201903333SA (en) * | 2017-12-29 | 2019-08-27 | Clear Labs Inc | Automated priming and library loading services |
| US10597714B2 (en) | 2017-12-29 | 2020-03-24 | Clear Labs, Inc. | Automated priming and library loading device |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| EP3820495A4 (en) | 2018-07-09 | 2022-07-20 | The Broad Institute Inc. | RNA-PROGRAMMABLE EPIGENETIC RNA MODIFIERS AND THEIR USES |
| CN108950033B (zh) * | 2018-07-26 | 2022-01-11 | 江苏省农业科学院 | 同时检测肠出血性大肠杆菌o45和o145的纳米pcr试剂盒及其应用 |
| WO2020051301A1 (en) * | 2018-09-06 | 2020-03-12 | Hygiena, Llc | Sequences and their use for detection and characterization of escherichia coli serotype o157:h7 |
| CN111100862B (zh) * | 2018-10-26 | 2023-09-29 | 厦门大学 | 一种检测细菌血清型的方法 |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| EP3921417A4 (en) | 2019-02-04 | 2022-11-09 | The General Hospital Corporation | VARIANTS OF ADENINE DNA BASES WITH REDUCED OFF-TARGET RNA EDIT |
| EP3942043A2 (en) | 2019-03-19 | 2022-01-26 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| WO2020214842A1 (en) | 2019-04-17 | 2020-10-22 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| BR112022022603A2 (pt) | 2020-05-08 | 2023-01-17 | Broad Inst Inc | Métodos e composições para edição simultânea de ambas as fitas de sequência alvo de nucleotídeos de fita dupla |
| US20230279442A1 (en) | 2021-12-15 | 2023-09-07 | Versitech Limited | Engineered cas9-nucleases and method of use thereof |
| CN116903710B (zh) * | 2023-09-12 | 2023-11-14 | 山东省农业科学院畜牧兽医研究所 | 噬菌体靶向蛋白分子及其在鉴定o103抗原血清型的产志贺毒素大肠杆菌中的应用 |
| WO2026006542A2 (en) | 2024-06-26 | 2026-01-02 | Yale University | Compositions and methods for crispr/cas9 based reactivation of human angelman syndrome |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002355074A (ja) * | 2001-01-24 | 2002-12-10 | Univ Tsukuba | 腸管出血性病原性大腸菌ｏ１５７：ｈ７に特異的な核酸分子およびポリペプチド並びにこれらの使用方法 |
| US20060051751A1 (en) * | 2002-01-23 | 2006-03-09 | Gehua Wang | Major virulence factor detection and verocytontoxin type 2 subtype from clinical e. coli isolates using a one-step multiplex pcr |
| EP2292799A1 (en) | 2009-08-11 | 2011-03-09 | Agence Française de Securité Sanitaire des Aliments | An assay for determining a molecular risk assessment of stec isolates |
-
2012
- 2012-06-14 EP EP12171941.3A patent/EP2674501A1/en not_active Withdrawn
-
2013
- 2013-06-14 EP EP13747516.6A patent/EP2861762B1/en active Active
- 2013-06-14 US US14/407,884 patent/US10190175B2/en active Active
- 2013-06-14 CA CA2875919A patent/CA2875919C/en active Active
- 2013-06-14 WO PCT/IB2013/054888 patent/WO2013186754A2/en not_active Ceased
- 2013-06-14 CN CN201380030965.9A patent/CN104619860B/zh active Active
- 2013-06-14 ES ES13747516.6T patent/ES2659469T3/es active Active
- 2013-06-14 BR BR112014031370-9A patent/BR112014031370B1/pt active IP Right Grant
- 2013-06-14 AU AU2013276084A patent/AU2013276084B2/en active Active
- 2013-06-14 JP JP2015516741A patent/JP6166365B2/ja active Active
- 2013-06-14 MX MX2014015130A patent/MX357697B/es active IP Right Grant
- 2013-06-14 KR KR1020157000638A patent/KR102065053B1/ko active Active
-
2014
- 2014-12-12 CL CL2014003377A patent/CL2014003377A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CN104619860B (zh) | 2018-01-30 |
| JP2015519075A (ja) | 2015-07-09 |
| MX2014015130A (es) | 2015-08-06 |
| CA2875919A1 (en) | 2013-12-19 |
| US20150176064A1 (en) | 2015-06-25 |
| ES2659469T3 (es) | 2018-03-15 |
| KR20150030783A (ko) | 2015-03-20 |
| AU2013276084A1 (en) | 2015-01-29 |
| JP6166365B2 (ja) | 2017-07-19 |
| BR112014031370A2 (pt) | 2017-07-25 |
| WO2013186754A3 (en) | 2014-02-20 |
| AU2013276084B2 (en) | 2019-04-04 |
| US10190175B2 (en) | 2019-01-29 |
| WO2013186754A2 (en) | 2013-12-19 |
| CL2014003377A1 (es) | 2015-08-07 |
| BR112014031370B1 (pt) | 2022-06-07 |
| EP2861762B1 (en) | 2017-12-13 |
| EP2674501A1 (en) | 2013-12-18 |
| EP2861762A2 (en) | 2015-04-22 |
| AU2013276084A8 (en) | 2015-02-12 |
| KR102065053B1 (ko) | 2020-01-10 |
| CN104619860A (zh) | 2015-05-13 |
| MX357697B (es) | 2018-07-19 |
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