MX357697B - Método para detectar e identificar escherichia colienterohemorrágica. - Google Patents

Método para detectar e identificar escherichia colienterohemorrágica.

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Publication number
MX357697B
MX357697B MX2014015130A MX2014015130A MX357697B MX 357697 B MX357697 B MX 357697B MX 2014015130 A MX2014015130 A MX 2014015130A MX 2014015130 A MX2014015130 A MX 2014015130A MX 357697 B MX357697 B MX 357697B
Authority
MX
Mexico
Prior art keywords
escherichia coli
detecting
enterohemorrhagic escherichia
identifying
ehec
Prior art date
Application number
MX2014015130A
Other languages
English (en)
Other versions
MX2014015130A (es
Inventor
Fach Patrick
Delannoy Sabine
Beutin Lothar
Original Assignee
Agence Nat De Securite Sanitaire De L´Alimentation De L´Environnement Et Du Travail
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by Agence Nat De Securite Sanitaire De L´Alimentation De L´Environnement Et Du Travail filed Critical Agence Nat De Securite Sanitaire De L´Alimentation De L´Environnement Et Du Travail
Publication of MX2014015130A publication Critical patent/MX2014015130A/es
Publication of MX357697B publication Critical patent/MX357697B/es

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/156—Polymorphic or mutational markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/158—Expression markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención se refiere a métodos para predecir si una muestra contiene o no Escherichia coli enterohemorrágica (EHEC) de al menos uno de los serotipos 0157: [H7], 0145: [H28], 0103: [H2], 0111: [H8], 0121: [H19], 026: [H11], 045: [H2] o 0104: [H4] de EHEC, y para identificar los serotipos, a través de la detección del genes espK en asociación con al menos uno de los marcadores genéticos Z1151, Z1153, Z1154, Z1155, Z1156, Z6065, Z2098, ureD o espV y/o a través de la detección de las secuencias CRISPR serotipo-específicas. El método comprende realizar una análisis de PCR a dicha muestra o ADN aislado de la mima con una combinación de cebadores dirigidos a dichas secuencias CRISPR.
MX2014015130A 2012-06-14 2013-06-14 Método para detectar e identificar escherichia colienterohemorrágica. MX357697B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP12171941.3A EP2674501A1 (en) 2012-06-14 2012-06-14 Method for detecting and identifying enterohemorrhagic Escherichia coli
PCT/IB2013/054888 WO2013186754A2 (en) 2012-06-14 2013-06-14 Method for detecting and identifying enterohemorrhagic escherichia coli

Publications (2)

Publication Number Publication Date
MX2014015130A MX2014015130A (es) 2015-08-06
MX357697B true MX357697B (es) 2018-07-19

Family

ID=48949190

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014015130A MX357697B (es) 2012-06-14 2013-06-14 Método para detectar e identificar escherichia colienterohemorrágica.

Country Status (12)

Country Link
US (1) US10190175B2 (es)
EP (2) EP2674501A1 (es)
JP (1) JP6166365B2 (es)
KR (1) KR102065053B1 (es)
CN (1) CN104619860B (es)
AU (1) AU2013276084B2 (es)
BR (1) BR112014031370B1 (es)
CA (1) CA2875919C (es)
CL (1) CL2014003377A1 (es)
ES (1) ES2659469T3 (es)
MX (1) MX357697B (es)
WO (1) WO2013186754A2 (es)

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Also Published As

Publication number Publication date
BR112014031370A2 (pt) 2017-07-25
KR20150030783A (ko) 2015-03-20
AU2013276084A8 (en) 2015-02-12
AU2013276084A1 (en) 2015-01-29
CA2875919C (en) 2021-08-31
US20150176064A1 (en) 2015-06-25
EP2861762A2 (en) 2015-04-22
CA2875919A1 (en) 2013-12-19
AU2013276084B2 (en) 2019-04-04
CL2014003377A1 (es) 2015-08-07
MX2014015130A (es) 2015-08-06
EP2674501A1 (en) 2013-12-18
BR112014031370B1 (pt) 2022-06-07
EP2861762B1 (en) 2017-12-13
JP6166365B2 (ja) 2017-07-19
WO2013186754A2 (en) 2013-12-19
CN104619860A (zh) 2015-05-13
US10190175B2 (en) 2019-01-29
ES2659469T3 (es) 2018-03-15
KR102065053B1 (ko) 2020-01-10
WO2013186754A3 (en) 2014-02-20
JP2015519075A (ja) 2015-07-09
CN104619860B (zh) 2018-01-30

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