MX2012000969A - Secuencias y su uso para deteccion y caracterizacion de o157:h7 de e.coli. - Google Patents

Secuencias y su uso para deteccion y caracterizacion de o157:h7 de e.coli.

Info

Publication number
MX2012000969A
MX2012000969A MX2012000969A MX2012000969A MX2012000969A MX 2012000969 A MX2012000969 A MX 2012000969A MX 2012000969 A MX2012000969 A MX 2012000969A MX 2012000969 A MX2012000969 A MX 2012000969A MX 2012000969 A MX2012000969 A MX 2012000969A
Authority
MX
Mexico
Prior art keywords
detection
characterization
coli
sequences
present
Prior art date
Application number
MX2012000969A
Other languages
English (en)
Inventor
Frank R Burns
Original Assignee
Du Pont
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont filed Critical Du Pont
Publication of MX2012000969A publication Critical patent/MX2012000969A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

Esta invención se refiere a un método rápido para detección y caracterización de serotipo O157:H7 de bacterias de Escherichia coli con base en la presencia de secuencias de ácido nucleico, en particular, a un método basado en PCR para detección, y a moléculas y reactivos de oligonucleótido y kits útiles para ello. Este método se emplea preferiblemente para detectar O157:H7 de E. coli en una muestra de alimento o agua, tal como un enriquecimiento de carne. La presente invención además se refiere a composiciones de replicación y kits para llevar a cabo el método de la presente invención.
MX2012000969A 2009-07-22 2010-07-20 Secuencias y su uso para deteccion y caracterizacion de o157:h7 de e.coli. MX2012000969A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US22762209P 2009-07-22 2009-07-22
PCT/US2010/042570 WO2011011391A1 (en) 2009-07-22 2010-07-20 Sequences and their use for detection and characterization of e. coli o157:h7

Publications (1)

Publication Number Publication Date
MX2012000969A true MX2012000969A (es) 2012-02-28

Family

ID=42782046

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2012000969A MX2012000969A (es) 2009-07-22 2010-07-20 Secuencias y su uso para deteccion y caracterizacion de o157:h7 de e.coli.

Country Status (8)

Country Link
US (4) US8846349B2 (es)
EP (1) EP2456884A1 (es)
JP (1) JP2013500008A (es)
AU (1) AU2010276352B2 (es)
BR (1) BR112012001411A2 (es)
CA (1) CA2768794C (es)
MX (1) MX2012000969A (es)
WO (1) WO2011011391A1 (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR112012001411A2 (pt) * 2009-07-22 2019-09-24 Du Pont sequencias e seu uso para detecção e caracterização de e. coli 0157:h7
ES2623859T3 (es) * 2010-03-04 2017-07-12 Miacom Diagnostics Gmbh FISH múltiple mejorada
AU2012315852B2 (en) 2011-09-28 2018-03-01 Qualicon Diagnostics, Llc Sequences and their use for detection and characterization of STEC bacteria
CN104066849B (zh) * 2011-10-11 2017-04-19 凯杰有限公司 样品处理方法和样品处理盒
US20170260576A1 (en) * 2012-02-24 2017-09-14 Seegene, Inc. Td probe and its uses
US20140038182A1 (en) 2012-07-17 2014-02-06 Dna Logix, Inc. Cooperative primers, probes, and applications thereof
RU2015103777A (ru) * 2012-07-17 2016-09-10 Дна Лоджикс, Инк. Кооперативные праймеры, зонды и их применения
EP2959012B1 (en) * 2013-02-21 2018-02-14 Qualicon Diagnostics LLC Sequences and their use for detection and characterization of e. coli o157:h7
US9451953B2 (en) * 2013-12-11 2016-09-27 Depuy Mitek, Llc Knotless collapsible sutures and methods for suturing
WO2016087621A1 (en) 2014-12-03 2016-06-09 Qiagen Gmbh Highly specific and sensitive methods for detecting enterohemorrhagic escherichia coli serotypes o157:h7 and/or o145:h28
US20210324452A1 (en) 2018-09-06 2021-10-21 Hygiena, Llc Sequences and their use for detection and characterization of escherichia coli serotype o157:h7
CN112481400A (zh) * 2020-12-24 2021-03-12 山西大学 一种食品病原菌致泻大肠埃希氏菌快速检测试剂盒及方法

Family Cites Families (39)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4762857A (en) 1986-05-30 1988-08-09 E. I. Du Pont De Nemours And Company Trehalose as stabilizer and tableting excipient
US4678812A (en) 1986-05-30 1987-07-07 E. I. Du Pont De Nemours And Company Trehalose as stabilizer and tableting excipient
CA1323293C (en) 1987-12-11 1993-10-19 Keith C. Backman Assay using template-dependent nucleic acid probe reorganization
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5538848A (en) 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
US5654417A (en) 1995-04-14 1997-08-05 Children's Hospital And Medical Center Nucleic acid probes for detecting E. coli O157:H7
ATE186750T1 (de) 1995-09-20 1999-12-15 Du Pont Eine kontrolle für die pcr
US6312930B1 (en) 1996-09-16 2001-11-06 E. I. Du Pont De Nemours And Company Method for detecting bacteria using PCR
DE69841642D1 (de) 1997-05-01 2010-06-10 Univ Sydney Nukleinsäure-moleküle spezifisch für bakterielle antigene und deren verwendungen
AUPP363498A0 (en) 1998-05-21 1998-06-11 University Of Sydney, The Antigens and their detection
GB9812768D0 (en) 1998-06-13 1998-08-12 Zeneca Ltd Methods
US20080184386A1 (en) 1998-11-16 2008-07-31 Yongwei Cao Plant genome sequences and uses thereof
US20080113342A1 (en) 1998-11-16 2008-05-15 Monsanto Technology Llc Plant Genome Sequence and Uses Thereof
US6365723B1 (en) 1998-12-04 2002-04-02 Wisconsin Alumni Research Foundation Sequences of E. coli O157
EP1185697A2 (en) 1999-04-30 2002-03-13 Qualicon, Inc. Assay based on the analysis of dna melting curves
WO2002052035A2 (en) 2000-10-30 2002-07-04 Paradigm Genetics, Inc. Methods for identification of inhibitors of cinnamyl alcohol dehydrogenase in plants
JP2002355074A (ja) 2001-01-24 2002-12-10 Univ Tsukuba 腸管出血性病原性大腸菌ｏ１５７：ｈ７に特異的な核酸分子およびポリペプチド並びにこれらの使用方法
DE60210697T2 (de) 2001-02-13 2007-04-05 Ajinomoto Co., Inc. Verfahren zur Produktion von L-Aminosäuren mittels Bakterien der Gattung Escherichia
US8119365B2 (en) 2002-01-23 2012-02-21 Wisconsin Alumni Research Foundation Insertion sequence-free bacteria
CA2474139A1 (en) * 2002-01-23 2003-07-31 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Health Major virulence factor detection and verocytotoxin type 2 subtype from clinical e. coli isolates using a one-step multiplex pcr
US20060094034A1 (en) 2003-04-30 2006-05-04 Roland Brousseau Virulence and antibiotic resistance array and uses thereof
WO2005087930A1 (en) 2004-03-11 2005-09-22 Warnex Research Inc. Polynucleotides for the detection of escherichia coli o157
US7785780B2 (en) 2004-03-19 2010-08-31 Universite Catholique De Louvain Assay for detecting and identifying micro-organisms
JP4782784B2 (ja) * 2004-06-25 2011-09-28 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー 病原性大腸菌の検出および識別のためのｄｎａ配列
CN100395348C (zh) 2004-08-20 2008-06-18 深圳太太基因工程有限公司 一种用于检测大肠杆菌o157：h7核苷酸片段的引物和探针序列
JPWO2006057341A1 (ja) 2004-11-26 2008-06-05 協和醗酵工業株式会社 工業的に有用な微生物
EP2460534A1 (en) 2004-12-22 2012-06-06 Cleveland Clinic Foundation Flagellin related polypeptides and uses thereof
CN100436596C (zh) 2005-02-05 2008-11-26 厦门大学 肠出血性大肠杆菌o157核酸检测方法及其检测试剂盒
US8178339B2 (en) 2005-08-20 2012-05-15 Scarab Genomics Llc Reduced genome E. coli
EP1770171A1 (en) 2005-09-29 2007-04-04 Universität Zu Köln DNA microarray for rapid identification of Candida albicans in blood cultures.
WO2008108805A2 (en) 2006-07-19 2008-09-12 North Carolina State University Attenuated fnr deficient enterobacteria
WO2008027560A2 (en) 2006-09-01 2008-03-06 Anza Therapeutics, Inc. Holin-enhanced vaccines and reagents, and methods of use thereof
CN101113475B (zh) 2007-05-30 2010-10-06 中国疾病预防控制中心传染病预防控制所 病原微生物检测用引物和使用所述引物的多重扩增
CN101113476B (zh) 2007-05-30 2010-05-26 中国疾病预防控制中心传染病预防控制所 一种病原微生物dna检测芯片及其制备方法和应用
CN101153331A (zh) 2007-09-21 2008-04-02 珠海市疾病预防控制中心 大肠杆菌o157:h7检测用引物、检测方法、检测试剂盒
BR112012001411A2 (pt) * 2009-07-22 2019-09-24 Du Pont sequencias e seu uso para detecção e caracterização de e. coli 0157:h7

Also Published As

Publication number Publication date
AU2010276352A1 (en) 2012-02-02
BR112012001411A2 (pt) 2019-09-24
US9481913B2 (en) 2016-11-01
US8846349B2 (en) 2014-09-30
US20140371441A1 (en) 2014-12-18
US20140371104A1 (en) 2014-12-18
AU2010276352B2 (en) 2016-05-19
CA2768794C (en) 2018-09-18
CA2768794A1 (en) 2011-01-27
WO2011011391A1 (en) 2011-01-27
US20170159110A1 (en) 2017-06-08
JP2013500008A (ja) 2013-01-07
US20110020823A1 (en) 2011-01-27
EP2456884A1 (en) 2012-05-30

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