MX2009010676A - Metodo para identificar que un alimento contiene cerdo. - Google Patents
Metodo para identificar que un alimento contiene cerdo.Info
- Publication number
- MX2009010676A MX2009010676A MX2009010676A MX2009010676A MX2009010676A MX 2009010676 A MX2009010676 A MX 2009010676A MX 2009010676 A MX2009010676 A MX 2009010676A MX 2009010676 A MX2009010676 A MX 2009010676A MX 2009010676 A MX2009010676 A MX 2009010676A
- Authority
- MX
- Mexico
- Prior art keywords
- dna
- pork
- meat
- reaction
- real
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6851—Quantitative amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2531/00—Reactions of nucleic acids characterised by
- C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
- C12Q2531/113—PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/124—Animal traits, i.e. production traits, including athletic performance or the like
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
En este estudio, se desarrolla un ensayo de PCR de tiempo real de tiempo real específico para cerdo para la autenticación de Halal. Se emplean tres especies de muestras cárnicas, que fueron cerdo, vaca, pollo. Estos tres tipos de carne están entre la carne más comúnmente consumida en Malasia y están fácilmente disponibles el mercado. Se extrajo exitosamente ADN de cada muestra de carne al natural usando el Equipo de sangre y tejido DNeasyMR (Quiagen, Hilden, Alemania). Se estima la concentración del ADN extraído por espectrofotometría de absorción UV usando el biofotometro (Eppendorf AG, Hamburg, Alemania) Antes de la reacción de PCR de tiempo real. La temperatura de fijación para los cebadores está a 58° C. Para verificar la especificidad de los cebadores diseñados, se lleva a cabo la reacción para probar los cebadores contra las otras dos muestras cárnicas para detectar posibles reacciones cruzadas. La reacción solo amplifico ADN de cerdo a Ct 22.83. El ensayo de PCR de tiempo real descrito en este artículo probó ser muy sensible con un límite bajo de detección cuando se probaron las muestras. El ensayo se hace al preparar una serie de diluciones de 10 veces iniciando desde 10º ng de ADN que se usaron para determinar la sensibilidad de la reacción. El umbral de sensibilidad fue hasta 0.001 ng de ADN de cerdo. Se ha reportado que un límite de detección de 0.1 ng de ADN de cerdo usando PCR convencional. En este contexto, el Método será útil en la detección de ADN porcino en productos alimenticios.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| MYPI20082327 MY149386A (en) | 2008-06-26 | 2008-06-26 | A method for identifying a pork content in a food |
| PCT/MY2009/000047 WO2009157750A1 (en) | 2008-06-26 | 2009-03-31 | A method for identifying a pork content in a food |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| MX2009010676A true MX2009010676A (es) | 2010-04-30 |
Family
ID=41444706
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| MX2009010676A MX2009010676A (es) | 2008-06-26 | 2009-03-31 | Metodo para identificar que un alimento contiene cerdo. |
Country Status (17)
| Country | Link |
|---|---|
| US (1) | US20100216136A1 (es) |
| EP (1) | EP2158314B1 (es) |
| JP (1) | JP5055565B2 (es) |
| KR (1) | KR101318311B1 (es) |
| CN (1) | CN101715488B (es) |
| AU (1) | AU2009227859B8 (es) |
| BR (1) | BRPI0902897A2 (es) |
| CA (1) | CA2685133C (es) |
| DK (1) | DK2158314T3 (es) |
| EG (1) | EG25651A (es) |
| ES (1) | ES2448585T3 (es) |
| MA (1) | MA31297B1 (es) |
| MX (1) | MX2009010676A (es) |
| MY (1) | MY149386A (es) |
| NZ (1) | NZ580051A (es) |
| WO (1) | WO2009157750A1 (es) |
| ZA (1) | ZA200907056B (es) |
Families Citing this family (40)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102213720A (zh) * | 2010-04-08 | 2011-10-12 | 青岛农业大学 | Sds-page法鉴别肉及肉制品中动物源性成份 |
| CN101962675B (zh) * | 2010-07-29 | 2013-02-06 | 天津大学 | 多重pcr检测食品中肉类来源的引物组及试剂盒 |
| WO2012059102A2 (en) * | 2010-11-05 | 2012-05-10 | Leo Pharma A/S | A method of detecting contaminant dna in biological samples |
| CN102433382B (zh) * | 2011-12-07 | 2013-10-23 | 上海出入境检验检疫局动植物与食品检验检疫技术中心 | 食品及饲料中火鸡成分的实时荧光pcr检测方法 |
| US20130269537A1 (en) | 2012-04-16 | 2013-10-17 | Eugenio Minvielle | Conditioning system for nutritional substances |
| US8490862B1 (en) | 2012-04-16 | 2013-07-23 | Eugenio Minvielle | Transformation system for nutritional substances |
| US20130269538A1 (en) | 2012-04-16 | 2013-10-17 | Eugenio Minvielle | Transformation system for nutritional substances |
| US9541536B2 (en) | 2012-04-16 | 2017-01-10 | Eugenio Minvielle | Preservation system for nutritional substances |
| US10219531B2 (en) | 2012-04-16 | 2019-03-05 | Iceberg Luxembourg S.A.R.L. | Preservation system for nutritional substances |
| US8550365B1 (en) | 2012-04-16 | 2013-10-08 | Eugenio Minvielle | System for managing the nutritional content for nutritional substances |
| US9460633B2 (en) | 2012-04-16 | 2016-10-04 | Eugenio Minvielle | Conditioner with sensors for nutritional substances |
| US9429920B2 (en) | 2012-04-16 | 2016-08-30 | Eugenio Minvielle | Instructions for conditioning nutritional substances |
| US9564064B2 (en) | 2012-04-16 | 2017-02-07 | Eugenio Minvielle | Conditioner with weight sensors for nutritional substances |
| US8851365B2 (en) | 2012-04-16 | 2014-10-07 | Eugenio Minvielle | Adaptive storage and conditioning systems for nutritional substances |
| US9436170B2 (en) | 2012-04-16 | 2016-09-06 | Eugenio Minvielle | Appliances with weight sensors for nutritional substances |
| US9414623B2 (en) | 2012-04-16 | 2016-08-16 | Eugenio Minvielle | Transformation and dynamic identification system for nutritional substances |
| US9080997B2 (en) | 2012-04-16 | 2015-07-14 | Eugenio Minvielle | Local storage and conditioning systems for nutritional substances |
| US9702858B1 (en) | 2012-04-16 | 2017-07-11 | Iceberg Luxembourg S.A.R.L. | Dynamic recipe control |
| US9121840B2 (en) | 2012-04-16 | 2015-09-01 | Eugenio Minvielle | Logistic transport system for nutritional substances |
| US9072317B2 (en) | 2012-04-16 | 2015-07-07 | Eugenio Minvielle | Transformation system for nutritional substances |
| US9016193B2 (en) | 2012-04-16 | 2015-04-28 | Eugenio Minvielle | Logistic transport system for nutritional substances |
| US8733631B2 (en) | 2012-04-16 | 2014-05-27 | Eugenio Minvielle | Local storage and conditioning systems for nutritional substances |
| US20140069838A1 (en) | 2012-04-16 | 2014-03-13 | Eugenio Minvielle | Nutritional Substance Label System For Adaptive Conditioning |
| US9069340B2 (en) | 2012-04-16 | 2015-06-30 | Eugenio Minvielle | Multi-conditioner control for conditioning nutritional substances |
| US9171061B2 (en) | 2012-04-16 | 2015-10-27 | Eugenio Minvielle | Local storage and conditioning systems for nutritional substances |
| US9528972B2 (en) | 2012-04-16 | 2016-12-27 | Eugenio Minvielle | Dynamic recipe control |
| EP2695092A4 (en) * | 2012-05-31 | 2014-03-26 | Eugenio Minvielle | SYSTEM AND METHOD FOR MONITORING NUTRITIONAL SUBSTANCES FOR DISPLAYING DISTURBANCES |
| CN102994637A (zh) * | 2012-11-26 | 2013-03-27 | 中国肉类食品综合研究中心 | 一种检测肉及肉制品中牛源性成分的方法 |
| CN103290105A (zh) * | 2013-02-01 | 2013-09-11 | 四川农业大学 | 一种猪源性成分实时荧光pcr检测方法及引物 |
| US10790062B2 (en) | 2013-10-08 | 2020-09-29 | Eugenio Minvielle | System for tracking and optimizing health indices |
| USD762081S1 (en) | 2014-07-29 | 2016-07-26 | Eugenio Minvielle | Device for food preservation and preparation |
| WO2016064264A1 (en) * | 2014-10-23 | 2016-04-28 | University Of Malaya | Multiplex pcr kit for the detection of pig, dog, cat, rat and monkey derived materials for halal authentication |
| CN105349626A (zh) * | 2015-10-09 | 2016-02-24 | 青岛农业大学 | 一种莱芜猪及其肉制品的鉴定方法 |
| DE102017200183A1 (de) | 2017-01-09 | 2018-07-12 | Robert Bosch Gmbh | Nucleinsäurebasierter Lateral Flow-Assay für schnelle Fleischspeziesbestimmung |
| CN106987647A (zh) * | 2017-05-25 | 2017-07-28 | 上海瑞丰农业科技有限公司 | 一种检测猪源性成分的rpa引物、试剂盒及检测方法 |
| CN108118086A (zh) * | 2018-01-30 | 2018-06-05 | 西北民族大学 | 用于检测肉制品中猪肉成分的rpa引物、探针及方法 |
| CN108624659B (zh) * | 2018-06-22 | 2021-06-08 | 武汉轻工大学 | 一种检测肉类制品成分的实时定量pcr方法 |
| WO2020089756A1 (en) * | 2018-10-30 | 2020-05-07 | Tubitak | Detection method for halal food and halal food additives |
| CN111334585B (zh) * | 2019-10-08 | 2022-06-28 | 齐鲁工业大学 | 同时检测8种动物成分的引物、试剂盒及检测方法和应用 |
| CN110904245B (zh) * | 2019-12-23 | 2023-08-15 | 中南民族大学 | 一种利用CACA基因鉴定猪肉成分的TaqMan荧光定量PCR方法及其应用 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10105056A1 (de) * | 2001-02-05 | 2002-08-08 | Congen Biotechnologie Gmbh | Verfahren und Kit zur tierartspezifischen DNA-Indifikation in einer Probe |
| JP4117370B2 (ja) * | 2001-11-30 | 2008-07-16 | 独立行政法人農林水産消費安全技術センター | プライマー配列 |
| GB0607712D0 (en) * | 2006-04-19 | 2006-05-31 | Sec Dep For Environment Food & | Detection assay |
| EP2027771B1 (en) * | 2007-08-24 | 2012-06-13 | Hermitage Pedigree Pigs Ltd. | Sequencing the mitochondrial DNA with reference to the fertility as a means for the optimization of sow breeding lines |
| CN101196463B (zh) * | 2007-12-14 | 2010-10-27 | 东北农业大学 | 一种动物源性成分的鉴别方法 |
-
2008
- 2008-06-26 MY MYPI20082327 patent/MY149386A/en unknown
-
2009
- 2009-03-31 EP EP20090748006 patent/EP2158314B1/en not_active Not-in-force
- 2009-03-31 CA CA 2685133 patent/CA2685133C/en not_active Expired - Fee Related
- 2009-03-31 NZ NZ580051A patent/NZ580051A/en not_active IP Right Cessation
- 2009-03-31 AU AU2009227859A patent/AU2009227859B8/en not_active Ceased
- 2009-03-31 WO PCT/MY2009/000047 patent/WO2009157750A1/en active Application Filing
- 2009-03-31 ES ES09748006T patent/ES2448585T3/es active Active
- 2009-03-31 DK DK09748006T patent/DK2158314T3/da active
- 2009-03-31 BR BRPI0902897-8A patent/BRPI0902897A2/pt not_active IP Right Cessation
- 2009-03-31 KR KR1020097022984A patent/KR101318311B1/ko active IP Right Grant
- 2009-03-31 CN CN2009800003153A patent/CN101715488B/zh not_active Expired - Fee Related
- 2009-03-31 US US12/594,164 patent/US20100216136A1/en not_active Abandoned
- 2009-03-31 MX MX2009010676A patent/MX2009010676A/es active IP Right Grant
- 2009-03-31 JP JP2010518134A patent/JP5055565B2/ja not_active Expired - Fee Related
- 2009-10-02 MA MA32252A patent/MA31297B1/fr unknown
- 2009-10-09 ZA ZA2009/07056A patent/ZA200907056B/en unknown
- 2009-10-15 EG EG2009101521A patent/EG25651A/xx active
Also Published As
| Publication number | Publication date |
|---|---|
| EP2158314A1 (en) | 2010-03-03 |
| AU2009227859B8 (en) | 2014-07-24 |
| JP2010530763A (ja) | 2010-09-16 |
| CN101715488A (zh) | 2010-05-26 |
| WO2009157750A1 (en) | 2009-12-30 |
| JP5055565B2 (ja) | 2012-10-24 |
| AU2009227859B2 (en) | 2014-03-13 |
| EP2158314B1 (en) | 2013-12-18 |
| EG25651A (en) | 2012-05-02 |
| AU2009227859A1 (en) | 2010-01-14 |
| KR101318311B1 (ko) | 2013-10-15 |
| BRPI0902897A2 (pt) | 2015-06-23 |
| ZA200907056B (en) | 2011-03-30 |
| EP2158314A4 (en) | 2010-08-25 |
| CN101715488B (zh) | 2012-07-18 |
| MY149386A (en) | 2013-08-30 |
| CA2685133A1 (en) | 2009-12-26 |
| KR20110020706A (ko) | 2011-03-03 |
| AU2009227859A8 (en) | 2014-07-24 |
| DK2158314T3 (da) | 2014-02-17 |
| ES2448585T3 (es) | 2014-03-14 |
| US20100216136A1 (en) | 2010-08-26 |
| CA2685133C (en) | 2014-07-08 |
| NZ580051A (en) | 2011-09-30 |
| MA31297B1 (fr) | 2010-04-01 |
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| FG | Grant or registration |