BR112014031370B1 - Método para a identificação de serotipo(s) de escherichia coli enterohemorrágica em uma amostra - Google Patents
Método para a identificação de serotipo(s) de escherichia coli enterohemorrágica em uma amostra Download PDFInfo
- Publication number
- BR112014031370B1 BR112014031370B1 BR112014031370-9A BR112014031370A BR112014031370B1 BR 112014031370 B1 BR112014031370 B1 BR 112014031370B1 BR 112014031370 A BR112014031370 A BR 112014031370A BR 112014031370 B1 BR112014031370 B1 BR 112014031370B1
- Authority
- BR
- Brazil
- Prior art keywords
- ehec
- seq
- primers
- crispr
- sequence
- Prior art date
Links
- 241000588724 Escherichia coli Species 0.000 title claims abstract description 123
- 238000000034 method Methods 0.000 title claims abstract description 37
- 101150082166 espK gene Proteins 0.000 claims abstract description 104
- 101100021923 Helicobacter pylori (strain ATCC 700392 / 26695) lspA gene Proteins 0.000 claims abstract description 41
- 101150070667 ureD gene Proteins 0.000 claims abstract description 41
- 108091033409 CRISPR Proteins 0.000 claims abstract 35
- 238000010354 CRISPR gene editing Methods 0.000 claims abstract 35
- 238000001514 detection method Methods 0.000 claims description 94
- 230000003321 amplification Effects 0.000 claims description 80
- 238000003199 nucleic acid amplification method Methods 0.000 claims description 80
- 230000008685 targeting Effects 0.000 claims description 41
- 108090000623 proteins and genes Proteins 0.000 claims description 32
- 238000010222 PCR analysis Methods 0.000 claims description 24
- 108020004414 DNA Proteins 0.000 claims description 18
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 claims description 4
- 238000002944 PCR assay Methods 0.000 claims 2
- 230000002068 genetic effect Effects 0.000 abstract description 61
- 239000013615 primer Substances 0.000 description 107
- 239000000523 sample Substances 0.000 description 80
- 108010079723 Shiga Toxin Proteins 0.000 description 24
- 230000001717 pathogenic effect Effects 0.000 description 23
- 230000035945 sensitivity Effects 0.000 description 19
- 101150107911 eae gene Proteins 0.000 description 18
- 101100500479 Hafnia alvei eaeA gene Proteins 0.000 description 17
- 238000004458 analytical method Methods 0.000 description 16
- 238000009826 distribution Methods 0.000 description 14
- 238000003753 real-time PCR Methods 0.000 description 13
- 230000001018 virulence Effects 0.000 description 12
- 206010014896 Enterocolitis haemorrhagic Diseases 0.000 description 10
- 208000032759 Hemolytic-Uremic Syndrome Diseases 0.000 description 10
- 238000013459 approach Methods 0.000 description 10
- -1 phages or plasmids Chemical class 0.000 description 10
- 239000002987 primer (paints) Substances 0.000 description 10
- 235000013305 food Nutrition 0.000 description 9
- 102000004169 proteins and genes Human genes 0.000 description 9
- 108091028043 Nucleic acid sequence Proteins 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 8
- 208000015181 infectious disease Diseases 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 241000282412 Homo Species 0.000 description 7
- 239000003550 marker Substances 0.000 description 7
- 201000010099 disease Diseases 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 239000012636 effector Substances 0.000 description 6
- 230000036541 health Effects 0.000 description 6
- 238000012163 sequencing technique Methods 0.000 description 6
- 238000012408 PCR amplification Methods 0.000 description 4
- 238000011002 quantification Methods 0.000 description 4
- 125000006850 spacer group Chemical group 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 102000053602 DNA Human genes 0.000 description 3
- 101100001161 Escherichia coli aggR gene Proteins 0.000 description 3
- 108010046334 Urease Proteins 0.000 description 3
- 230000037029 cross reaction Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 230000000369 enteropathogenic effect Effects 0.000 description 3
- 239000003999 initiator Substances 0.000 description 3
- 238000011835 investigation Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 238000003752 polymerase chain reaction Methods 0.000 description 3
- 238000001712 DNA sequencing Methods 0.000 description 2
- 206010012735 Diarrhoea Diseases 0.000 description 2
- 101710201576 Putative membrane protein Proteins 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000002759 chromosomal effect Effects 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 210000004901 leucine-rich repeat Anatomy 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 239000013612 plasmid Substances 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000011895 specific detection Methods 0.000 description 2
- WCKQPPQRFNHPRJ-UHFFFAOYSA-N 4-[[4-(dimethylamino)phenyl]diazenyl]benzoic acid Chemical compound C1=CC(N(C)C)=CC=C1N=NC1=CC=C(C(O)=O)C=C1 WCKQPPQRFNHPRJ-UHFFFAOYSA-N 0.000 description 1
- COCMHKNAGZHBDZ-UHFFFAOYSA-N 4-carboxy-3-[3-(dimethylamino)-6-dimethylazaniumylidenexanthen-9-yl]benzoate Chemical compound C=12C=CC(=[N+](C)C)C=C2OC2=CC(N(C)C)=CC=C2C=1C1=CC(C([O-])=O)=CC=C1C(O)=O COCMHKNAGZHBDZ-UHFFFAOYSA-N 0.000 description 1
- WQZIDRAQTRIQDX-UHFFFAOYSA-N 6-carboxy-x-rhodamine Chemical compound OC(=O)C1=CC=C(C([O-])=O)C=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 WQZIDRAQTRIQDX-UHFFFAOYSA-N 0.000 description 1
- 108091093088 Amplicon Proteins 0.000 description 1
- 241000589877 Campylobacter coli Species 0.000 description 1
- 244000135860 Capparis spinosa subsp spinosa Species 0.000 description 1
- 101100516697 Citrobacter rodentium nleB gene Proteins 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 108020001019 DNA Primers Proteins 0.000 description 1
- 239000003155 DNA primer Substances 0.000 description 1
- 239000003298 DNA probe Substances 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000059459 Escherichia coli O104 Species 0.000 description 1
- 241001036088 Escherichia coli O104:H4 Species 0.000 description 1
- 101100240631 Escherichia coli O127:H6 (strain E2348/69 / EPEC) nleB1 gene Proteins 0.000 description 1
- 241001646719 Escherichia coli O157:H7 Species 0.000 description 1
- 101710116034 Immunity protein Proteins 0.000 description 1
- 238000007397 LAMP assay Methods 0.000 description 1
- 239000006137 Luria-Bertani broth Substances 0.000 description 1
- 101150000110 Nle gene Proteins 0.000 description 1
- 108020005187 Oligonucleotide Probes Proteins 0.000 description 1
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 1
- 108010017898 Shiga Toxins Proteins 0.000 description 1
- 230000001594 aberrant effect Effects 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010804 cDNA synthesis Methods 0.000 description 1
- 244000309466 calf Species 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 108010056602 colicin immunity proteins Proteins 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 210000001842 enterocyte Anatomy 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 230000002550 fecal effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 210000003495 flagella Anatomy 0.000 description 1
- 238000012268 genome sequencing Methods 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 230000005802 health problem Effects 0.000 description 1
- 208000031169 hemorrhagic disease Diseases 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000002687 intercalation Effects 0.000 description 1
- 238000009830 intercalation Methods 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000010606 normalization Methods 0.000 description 1
- 239000002751 oligonucleotide probe Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000007918 pathogenicity Effects 0.000 description 1
- 230000002688 persistence Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000012502 risk assessment Methods 0.000 description 1
- 238000011896 sensitive detection Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 101150080727 stx gene Proteins 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000012070 whole genome sequencing analysis Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12171941.3A EP2674501A1 (en) | 2012-06-14 | 2012-06-14 | Method for detecting and identifying enterohemorrhagic Escherichia coli |
| EP12171941.3 | 2012-06-14 | ||
| PCT/IB2013/054888 WO2013186754A2 (en) | 2012-06-14 | 2013-06-14 | Method for detecting and identifying enterohemorrhagic escherichia coli |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR112014031370A2 BR112014031370A2 (pt) | 2017-07-25 |
| BR112014031370B1 true BR112014031370B1 (pt) | 2022-06-07 |
Family
ID=48949190
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112014031370-9A BR112014031370B1 (pt) | 2012-06-14 | 2013-06-14 | Método para a identificação de serotipo(s) de escherichia coli enterohemorrágica em uma amostra |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US10190175B2 (enExample) |
| EP (2) | EP2674501A1 (enExample) |
| JP (1) | JP6166365B2 (enExample) |
| KR (1) | KR102065053B1 (enExample) |
| CN (1) | CN104619860B (enExample) |
| AU (1) | AU2013276084B2 (enExample) |
| BR (1) | BR112014031370B1 (enExample) |
| CA (1) | CA2875919C (enExample) |
| CL (1) | CL2014003377A1 (enExample) |
| ES (1) | ES2659469T3 (enExample) |
| MX (1) | MX357697B (enExample) |
| WO (1) | WO2013186754A2 (enExample) |
Families Citing this family (55)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2734621B1 (en) | 2011-07-22 | 2019-09-04 | President and Fellows of Harvard College | Evaluation and improvement of nuclease cleavage specificity |
| GB201122458D0 (en) | 2011-12-30 | 2012-02-08 | Univ Wageningen | Modified cascade ribonucleoproteins and uses thereof |
| SI3401400T1 (sl) | 2012-05-25 | 2019-10-30 | Univ California | Postopki in sestavki za RNA usmerjeno modifikacijo tarčne DNA in za RNA usmerjeno modulacijo prepisovanja |
| EP3138911B1 (en) | 2012-12-06 | 2018-12-05 | Sigma Aldrich Co. LLC | Crispr-based genome modification and regulation |
| US20140315985A1 (en) | 2013-03-14 | 2014-10-23 | Caribou Biosciences, Inc. | Compositions and methods of nucleic acid-targeting nucleic acids |
| US9885033B2 (en) | 2013-03-15 | 2018-02-06 | The General Hospital Corporation | Increasing specificity for RNA-guided genome editing |
| US10760064B2 (en) | 2013-03-15 | 2020-09-01 | The General Hospital Corporation | RNA-guided targeting of genetic and epigenomic regulatory proteins to specific genomic loci |
| US10011850B2 (en) | 2013-06-21 | 2018-07-03 | The General Hospital Corporation | Using RNA-guided FokI Nucleases (RFNs) to increase specificity for RNA-Guided Genome Editing |
| US20150044192A1 (en) | 2013-08-09 | 2015-02-12 | President And Fellows Of Harvard College | Methods for identifying a target site of a cas9 nuclease |
| US9526784B2 (en) | 2013-09-06 | 2016-12-27 | President And Fellows Of Harvard College | Delivery system for functional nucleases |
| US9340800B2 (en) | 2013-09-06 | 2016-05-17 | President And Fellows Of Harvard College | Extended DNA-sensing GRNAS |
| US9388430B2 (en) | 2013-09-06 | 2016-07-12 | President And Fellows Of Harvard College | Cas9-recombinase fusion proteins and uses thereof |
| CA2930015A1 (en) | 2013-11-07 | 2015-05-14 | Editas Medicine, Inc. | Crispr-related methods and compositions with governing grnas |
| US9840699B2 (en) | 2013-12-12 | 2017-12-12 | President And Fellows Of Harvard College | Methods for nucleic acid editing |
| WO2016022363A2 (en) | 2014-07-30 | 2016-02-11 | President And Fellows Of Harvard College | Cas9 proteins including ligand-dependent inteins |
| US10920285B2 (en) | 2014-12-03 | 2021-02-16 | Qiagen Gmbh | Highly specific and sensitive methods for detecting enterohemorrhagic Escherichia coli serotypes O157:H7 and/or O145:H28 |
| WO2016115441A1 (en) * | 2015-01-16 | 2016-07-21 | Kansas State University Research Foundation | Co-detection and association of multiple genes from the same genome in a sample |
| US9512446B1 (en) | 2015-08-28 | 2016-12-06 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| KR102668608B1 (ko) | 2015-08-28 | 2024-05-24 | 더 제너럴 하스피탈 코포레이션 | 조작된 crispr-cas9 뉴클레아제 |
| US9926546B2 (en) | 2015-08-28 | 2018-03-27 | The General Hospital Corporation | Engineered CRISPR-Cas9 nucleases |
| IL258821B (en) | 2015-10-23 | 2022-07-01 | Harvard College | Nucleobase editors and their uses |
| CN105779584A (zh) * | 2015-12-18 | 2016-07-20 | 珠海出入境检验检疫局检验检疫技术中心 | 大肠杆菌o111检测用的引物和探针组、试剂盒及pcr检测方法 |
| CN105821124A (zh) * | 2016-04-06 | 2016-08-03 | 山东省滨州畜牧兽医研究院 | 一种大肠杆菌 o26、o45、o121血清型三重pcr检测试剂盒及其引物组 |
| KR20250103795A (ko) | 2016-08-03 | 2025-07-07 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 아데노신 핵염기 편집제 및 그의 용도 |
| CA3033327A1 (en) | 2016-08-09 | 2018-02-15 | President And Fellows Of Harvard College | Programmable cas9-recombinase fusion proteins and uses thereof |
| WO2018039438A1 (en) | 2016-08-24 | 2018-03-01 | President And Fellows Of Harvard College | Incorporation of unnatural amino acids into proteins using base editing |
| JP7588390B2 (ja) | 2016-10-14 | 2024-11-22 | プレジデント アンド フェローズ オブ ハーバード カレッジ | 核酸塩基エディターのａａｖ送達 |
| WO2018119359A1 (en) | 2016-12-23 | 2018-06-28 | President And Fellows Of Harvard College | Editing of ccr5 receptor gene to protect against hiv infection |
| WO2018165504A1 (en) | 2017-03-09 | 2018-09-13 | President And Fellows Of Harvard College | Suppression of pain by gene editing |
| CN110662556A (zh) | 2017-03-09 | 2020-01-07 | 哈佛大学的校长及成员们 | 癌症疫苗 |
| JP2020510439A (ja) | 2017-03-10 | 2020-04-09 | プレジデント アンド フェローズ オブ ハーバード カレッジ | シトシンからグアニンへの塩基編集因子 |
| BR112019019655A2 (pt) | 2017-03-23 | 2020-04-22 | Harvard College | editores de nucleobase que compreendem proteínas de ligação a dna programáveis por ácido nucleico |
| CN110799525A (zh) | 2017-04-21 | 2020-02-14 | 通用医疗公司 | 具有改变的PAM特异性的CPF1(CAS12a)的变体 |
| WO2018209320A1 (en) | 2017-05-12 | 2018-11-15 | President And Fellows Of Harvard College | Aptazyme-embedded guide rnas for use with crispr-cas9 in genome editing and transcriptional activation |
| JP7324713B2 (ja) | 2017-05-25 | 2023-08-10 | ザ ジェネラル ホスピタル コーポレイション | 改善された精度および特異性を有する塩基エディター |
| JP2020534795A (ja) | 2017-07-28 | 2020-12-03 | プレジデント アンド フェローズ オブ ハーバード カレッジ | ファージによって支援される連続的進化（ｐａｃｅ）を用いて塩基編集因子を進化させるための方法および組成物 |
| US11319532B2 (en) | 2017-08-30 | 2022-05-03 | President And Fellows Of Harvard College | High efficiency base editors comprising Gam |
| KR20250107288A (ko) | 2017-10-16 | 2025-07-11 | 더 브로드 인스티튜트, 인코퍼레이티드 | 아데노신 염기 편집제의 용도 |
| WO2019118949A1 (en) | 2017-12-15 | 2019-06-20 | The Broad Institute, Inc. | Systems and methods for predicting repair outcomes in genetic engineering |
| WO2019133756A1 (en) * | 2017-12-29 | 2019-07-04 | Clear Labs, Inc. | Automated priming and library loading device |
| US10597714B2 (en) | 2017-12-29 | 2020-03-24 | Clear Labs, Inc. | Automated priming and library loading device |
| US10101328B1 (en) | 2017-12-29 | 2018-10-16 | Clear Labs, Inc. | Detection of microorganisms in food samples and food processing facilities |
| WO2019226953A1 (en) | 2018-05-23 | 2019-11-28 | The Broad Institute, Inc. | Base editors and uses thereof |
| CN108950033B (zh) * | 2018-07-26 | 2022-01-11 | 江苏省农业科学院 | 同时检测肠出血性大肠杆菌o45和o145的纳米pcr试剂盒及其应用 |
| US20210324452A1 (en) * | 2018-09-06 | 2021-10-21 | Hygiena, Llc | Sequences and their use for detection and characterization of escherichia coli serotype o157:h7 |
| CN111100862B (zh) * | 2018-10-26 | 2023-09-29 | 厦门大学 | 一种检测细菌血清型的方法 |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| WO2020163396A1 (en) | 2019-02-04 | 2020-08-13 | The General Hospital Corporation | Adenine dna base editor variants with reduced off-target rna editing |
| EP3942042A1 (en) | 2019-03-19 | 2022-01-26 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| EP3956349A1 (en) | 2019-04-17 | 2022-02-23 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| WO2021226558A1 (en) | 2020-05-08 | 2021-11-11 | The Broad Institute, Inc. | Methods and compositions for simultaneous editing of both strands of a target double-stranded nucleotide sequence |
| US20230279442A1 (en) | 2021-12-15 | 2023-09-07 | Versitech Limited | Engineered cas9-nucleases and method of use thereof |
| CN116903710B (zh) * | 2023-09-12 | 2023-11-14 | 山东省农业科学院畜牧兽医研究所 | 噬菌体靶向蛋白分子及其在鉴定o103抗原血清型的产志贺毒素大肠杆菌中的应用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002355074A (ja) * | 2001-01-24 | 2002-12-10 | Univ Tsukuba | 腸管出血性病原性大腸菌ｏ１５７：ｈ７に特異的な核酸分子およびポリペプチド並びにこれらの使用方法 |
| US20060051751A1 (en) * | 2002-01-23 | 2006-03-09 | Gehua Wang | Major virulence factor detection and verocytontoxin type 2 subtype from clinical e. coli isolates using a one-step multiplex pcr |
| EP2292799A1 (en) | 2009-08-11 | 2011-03-09 | Agence Française de Securité Sanitaire des Aliments | An assay for determining a molecular risk assessment of stec isolates |
-
2012
- 2012-06-14 EP EP12171941.3A patent/EP2674501A1/en not_active Withdrawn
-
2013
- 2013-06-14 MX MX2014015130A patent/MX357697B/es active IP Right Grant
- 2013-06-14 WO PCT/IB2013/054888 patent/WO2013186754A2/en not_active Ceased
- 2013-06-14 EP EP13747516.6A patent/EP2861762B1/en active Active
- 2013-06-14 BR BR112014031370-9A patent/BR112014031370B1/pt active IP Right Grant
- 2013-06-14 JP JP2015516741A patent/JP6166365B2/ja active Active
- 2013-06-14 CN CN201380030965.9A patent/CN104619860B/zh active Active
- 2013-06-14 ES ES13747516.6T patent/ES2659469T3/es active Active
- 2013-06-14 US US14/407,884 patent/US10190175B2/en active Active
- 2013-06-14 KR KR1020157000638A patent/KR102065053B1/ko active Active
- 2013-06-14 CA CA2875919A patent/CA2875919C/en active Active
- 2013-06-14 AU AU2013276084A patent/AU2013276084B2/en active Active
-
2014
- 2014-12-12 CL CL2014003377A patent/CL2014003377A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| MX357697B (es) | 2018-07-19 |
| BR112014031370A2 (pt) | 2017-07-25 |
| KR20150030783A (ko) | 2015-03-20 |
| AU2013276084A8 (en) | 2015-02-12 |
| AU2013276084A1 (en) | 2015-01-29 |
| CA2875919C (en) | 2021-08-31 |
| US20150176064A1 (en) | 2015-06-25 |
| EP2861762A2 (en) | 2015-04-22 |
| CA2875919A1 (en) | 2013-12-19 |
| AU2013276084B2 (en) | 2019-04-04 |
| CL2014003377A1 (es) | 2015-08-07 |
| MX2014015130A (es) | 2015-08-06 |
| EP2674501A1 (en) | 2013-12-18 |
| EP2861762B1 (en) | 2017-12-13 |
| JP6166365B2 (ja) | 2017-07-19 |
| WO2013186754A2 (en) | 2013-12-19 |
| CN104619860A (zh) | 2015-05-13 |
| US10190175B2 (en) | 2019-01-29 |
| ES2659469T3 (es) | 2018-03-15 |
| KR102065053B1 (ko) | 2020-01-10 |
| WO2013186754A3 (en) | 2014-02-20 |
| JP2015519075A (ja) | 2015-07-09 |
| CN104619860B (zh) | 2018-01-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| BR112014031370B1 (pt) | Método para a identificação de serotipo(s) de escherichia coli enterohemorrágica em uma amostra | |
| Perelle et al. | Detection by 5′-nuclease PCR of Shiga-toxin producing Escherichia coli O26, O55, O91, O103, O111, O113, O145 and O157: H7, associated with the world's most frequent clinical cases | |
| LaGier et al. | A real-time multiplexed PCR assay for rapid detection and differentiation of Campylobacter jejuni and Campylobacter coli | |
| US9481913B2 (en) | Sequences and their use for detection and characterization of E. coli O157:H7 | |
| Liu et al. | High-resolution melt curve PCR assay for specific detection of E. coli O157: H7 in beef | |
| Madic et al. | Simplex and multiplex real‐time PCR assays for the detection of flagellar (H‐antigen) fliC alleles and intimin (eae) variants associated with enterohaemorrhagic Escherichia coli (EHEC) serotypes O26: H11, O103: H2, O111: H8, O145: H28 and O157: H7 | |
| JP5990099B2 (ja) | Ｅｈｅｃを含有すると疑われる複雑な多菌サンプルの分子的リスク評価を決定するアッセイ | |
| CA2920636A1 (en) | Sequences and their use for detection of salmonella enteritidis and/or salmonella typhimurium | |
| Ferdous | Shiga toxin-producing Escherichia coli (STEC) from Humans in the Netherlands: Novel diagnostic approach, molecular characterization and phylogenetic background | |
| Elder | Serogrouping of clinically significant Shiga toxin-producing Escherichia coli by single nucleotide polymorphisms in the GND gene |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B06I | Publication of requirement cancelled [chapter 6.9 patent gazette] |
Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2462 DE 13/03/2018 POR TER SIDO INDEVIDA. |
|
| B06U | Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette] | ||
| B350 | Update of information on the portal [chapter 15.35 patent gazette] | ||
| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 14/06/2013, OBSERVADAS AS CONDICOES LEGAIS |