CA2806858C - Reprogramming immortalized b cells - Google Patents
Reprogramming immortalized b cells Download PDFInfo
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- CA2806858C CA2806858C CA2806858A CA2806858A CA2806858C CA 2806858 C CA2806858 C CA 2806858C CA 2806858 A CA2806858 A CA 2806858A CA 2806858 A CA2806858 A CA 2806858A CA 2806858 C CA2806858 C CA 2806858C
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- cells
- pluripotent stem
- induced pluripotent
- reprogramming
- immortalized
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- C—CHEMISTRY; METALLURGY
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- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C12N2501/235—Leukemia inhibitory factor [LIF]
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- C12N2501/603—Oct-3/4
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- C12N2501/604—Klf-4
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- C12N2501/605—Nanog
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/606—Transcription factors c-Myc
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/60—Transcription factors
- C12N2501/608—Lin28
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- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/70—Enzymes
- C12N2501/72—Transferases [EC 2.]
- C12N2501/727—Kinases (EC 2.7.)
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
- C12N2506/11—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from blood or immune system cells
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- C12N2510/00—Genetically modified cells
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- C12N2510/00—Genetically modified cells
- C12N2510/04—Immortalised cells
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- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16211—Lymphocryptovirus, e.g. human herpesvirus 4, Epstein-Barr Virus
- C12N2710/16241—Use of virus, viral particle or viral elements as a vector
- C12N2710/16243—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
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- C12N2800/00—Nucleic acids vectors
- C12N2800/10—Plasmid DNA
- C12N2800/108—Plasmid DNA episomal vectors
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Developmental Biology & Embryology (AREA)
- Transplantation (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Materials For Medical Uses (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US37061510P | 2010-08-04 | 2010-08-04 | |
| US61/370,615 | 2010-08-04 | ||
| US201161441885P | 2011-02-11 | 2011-02-11 | |
| US61/441,885 | 2011-02-11 | ||
| PCT/US2011/046452 WO2012018933A2 (en) | 2010-08-04 | 2011-08-03 | Reprogramming immortalized b cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2806858A1 CA2806858A1 (en) | 2012-02-09 |
| CA2806858C true CA2806858C (en) | 2021-06-15 |
Family
ID=45560057
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2806858A Active CA2806858C (en) | 2010-08-04 | 2011-08-03 | Reprogramming immortalized b cells |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US8765470B2 (https=) |
| EP (1) | EP2601289B1 (https=) |
| JP (2) | JP5936218B2 (https=) |
| CA (1) | CA2806858C (https=) |
| WO (1) | WO2012018933A2 (https=) |
Families Citing this family (56)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2010141801A2 (en) | 2009-06-05 | 2010-12-09 | Cellular Dynamics International, Inc. | Reprogramming t cells and hematophietic cells |
| CA2770412C (en) | 2009-08-07 | 2018-09-11 | Kyoto University | Method of efficiently establishing induced pluripotent stem cells |
| EP2655601A4 (en) | 2010-12-22 | 2014-09-10 | Fate Therapeutics Inc | CELL CULTURAL PLATFORM FOR INDIVIDUAL CELL SIZING AND IMPROVED RESOLUTION OF IPSCS |
| JP2014520551A (ja) * | 2011-07-11 | 2014-08-25 | セルラー ダイナミクス インターナショナル, インコーポレイテッド | 細胞のリプログラミング方法およびゲノムの改変方法 |
| CN102604894B (zh) * | 2012-02-29 | 2014-07-30 | 中国科学院广州生物医药与健康研究院 | 用于制备神经干细胞的培养基及其用途 |
| AU2013264708B2 (en) | 2012-05-23 | 2019-03-07 | Kyoto University | Highly efficient method for establishing artificial pluripotent stem cell |
| WO2014152832A1 (en) | 2013-03-14 | 2014-09-25 | Immusoft Corporation | Methods for in vitro memory b cell differentiation and transduction with vsv-g pseudotyped viral vectors |
| WO2014186766A1 (en) * | 2013-05-17 | 2014-11-20 | The Broad Institute, Inc. | Reprogrammed cells and methods of production and use thereof |
| US10738323B2 (en) | 2013-07-12 | 2020-08-11 | Cedars-Sinai Medical Center | Generation of induced pluripotent stem cells from normal human mammary epithelial cells |
| CA2941004A1 (en) | 2014-03-04 | 2015-09-11 | Peter Flynn | Improved reprogramming methods and cell culture platforms |
| CN105154386B (zh) * | 2014-05-30 | 2018-04-24 | 中国人民解放军第二军医大学东方肝胆外科医院 | 人肝细胞长期维持和增殖传代培养的专用培养基和培养方法 |
| DK3234107T3 (da) * | 2014-12-19 | 2022-10-17 | Immusoft Corp | B-celler til in vivo-levering af terapeutiske midler |
| KR101768581B1 (ko) * | 2015-08-13 | 2017-08-17 | 전북대학교 산학협력단 | 자발적 불멸화된 체세포의 단일 세포로부터 리프로그래밍 세포주의 제조방법 |
| CN117737124A (zh) | 2015-10-16 | 2024-03-22 | 菲特治疗公司 | 用于诱导和维护基态多能性的平台 |
| WO2017123789A1 (en) * | 2016-01-12 | 2017-07-20 | Lonza Walkersville, Inc. | Methods and vectors to produce vector free induced pluripotent stem cells |
| US10213511B2 (en) | 2016-03-02 | 2019-02-26 | Frequency Therapeutics, Inc. | Thermoreversible compositions for administration of therapeutic agents |
| US20190060371A1 (en) * | 2016-03-02 | 2019-02-28 | Frequency Therapeutics, Inc. | Methods for controlled proliferation of vestibular stem cells / generating inner ear hair cells using wnt and tgfbeta-inhibition |
| US10201540B2 (en) | 2016-03-02 | 2019-02-12 | Frequency Therapeutics, Inc. | Solubilized compositions for controlled proliferation of stem cells / generating inner ear hair cells using GSK3 inhibitors: I |
| US11185555B2 (en) | 2016-04-11 | 2021-11-30 | Noah James Harrison | Method to kill pathogenic microbes in a patient |
| WO2017217756A2 (ko) * | 2016-06-15 | 2017-12-21 | 한국생명공학연구원 | 자살유전자를 포함한 에피솜 벡터 및 이를 이용한 리프로그래밍 방법 |
| US11572545B2 (en) | 2016-06-16 | 2023-02-07 | Cedars-Sinai Medical Center | Efficient method for reprogramming blood to induced pluripotent stem cells |
| US10221395B2 (en) | 2016-06-16 | 2019-03-05 | Cedars-Sinai Medical Center | Efficient method for reprogramming blood to induced pluripotent stem cells |
| US11525119B2 (en) | 2016-09-06 | 2022-12-13 | The Children's Medical Center Corporation | Immune cells derived from induced pluripotent stem cell |
| CN108148808B (zh) * | 2016-12-05 | 2020-12-11 | 同济大学 | 有助于诱导生成神经前体细胞的诱导培养基 |
| WO2018135902A1 (ko) * | 2017-01-19 | 2018-07-26 | 가톨릭대학교 산학협력단 | 줄기 세포로부터 분화 유도된 연골세포의 제조방법 |
| US10767164B2 (en) | 2017-03-30 | 2020-09-08 | The Research Foundation For The State University Of New York | Microenvironments for self-assembly of islet organoids from stem cells differentiation |
| CN110621323B (zh) * | 2017-05-02 | 2022-10-14 | 田边刚士 | 医药品组合物及化妆品组合物 |
| EP3621630A4 (en) | 2017-06-14 | 2021-03-10 | The Children's Medical Center | HEMATOPOIETIC STEM AND PRECURSOR CELLS FROM HEMOGENIC ENDOTHELIAL CELLS BY EPISOMAL PLASMID GENERAL TRANSFER |
| JP7554670B2 (ja) | 2017-10-11 | 2024-09-20 | フェイト セラピューティクス,インコーポレイテッド | 一時的かつ一過性プラスミドベクター発現システムを用いる細胞のリプログラミング |
| WO2019106163A1 (en) * | 2017-12-01 | 2019-06-06 | Cellectis | Reprogramming of genetically engineered primary immune cells |
| CN113881624A (zh) * | 2018-01-29 | 2022-01-04 | 中国科学院动物研究所 | 一种细胞诱导的方法 |
| JP2021533788A (ja) | 2018-08-17 | 2021-12-09 | フリークエンシー セラピューティクス インコーポレイテッド | Jag−1を上方制御することにより有毛細胞を生成するための組成物及び方法 |
| US11617745B2 (en) | 2018-08-17 | 2023-04-04 | Frequency Therapeutics, Inc. | Compositions and methods for generating hair cells by downregulating FOXO |
| JP2022513355A (ja) * | 2018-10-12 | 2022-02-07 | リニューロン リミテッド | 条件付き不死化に関する制御可能な導入遺伝子を含む人工多能性細胞 |
| JP2022527629A (ja) | 2019-04-05 | 2022-06-02 | アーリー・インコーポレイテッド | 合成バイオマーカーのための改良された方法及び組成物 |
| US12582726B1 (en) | 2019-04-05 | 2026-03-24 | Earli Inc. | Synthetic cancer-specific promoters |
| WO2021150919A1 (en) | 2020-01-23 | 2021-07-29 | The Children's Medical Center Corporation | Stroma-free t cell differentiation from human pluripotent stem cells |
| CA3175071A1 (en) | 2020-03-11 | 2021-09-16 | Bit Bio Limited | Method of generating hepatic cells |
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| JP2024514522A (ja) | 2021-04-07 | 2024-04-02 | センチュリー セラピューティクス,インコーポレイテッド | 遺伝子導入ベクターおよび細胞を操作する方法 |
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| JP2024539519A (ja) * | 2021-10-20 | 2024-10-28 | エクシール, インク. | 非ヒト霊長類の個別化されたゲノムアセンブリ及び人工多能性幹細胞株を前臨床評価に使用するための組成物及び方法 |
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| EP4519319A1 (en) | 2022-05-04 | 2025-03-12 | Century Therapeutics, Inc. | Cells engineered with an hla-e and hla-g transgene |
| US20250090582A1 (en) | 2022-06-08 | 2025-03-20 | Century Therapeutics, Inc. | Genetically engineered cells having anti-cd133 / anti-egfr chimeric antigen receptors, and uses thereof |
| JP2025522360A (ja) | 2022-06-08 | 2025-07-15 | センチュリー セラピューティクス,インコーポレイテッド | Cd16変異体およびnkg2dを発現する遺伝子操作した細胞ならびにその使用 |
| CN119698467A (zh) | 2022-06-08 | 2025-03-25 | 世纪治疗股份有限公司 | 衍生自遗传工程化以具有膜结合的il12的诱导多能干细胞的免疫效应细胞及其用途 |
| WO2024102838A1 (en) | 2022-11-09 | 2024-05-16 | Century Therapeutics, Inc. | Engineered interleukin-7 receptors and uses thereof |
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| EP2601289A4 (en) | 2014-01-22 |
| US8765470B2 (en) | 2014-07-01 |
| JP2016039828A (ja) | 2016-03-24 |
| JP2013534140A (ja) | 2013-09-02 |
| JP5936218B2 (ja) | 2016-06-22 |
| WO2012018933A3 (en) | 2012-05-10 |
| US20120058562A1 (en) | 2012-03-08 |
| US20140349397A1 (en) | 2014-11-27 |
| EP2601289B1 (en) | 2017-07-12 |
| EP2601289A2 (en) | 2013-06-12 |
| WO2012018933A2 (en) | 2012-02-09 |
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