CA2792433C - Full cold-pcr enrichment with reference blocking sequence - Google Patents

Full cold-pcr enrichment with reference blocking sequence Download PDF

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Publication number
CA2792433C
CA2792433C CA2792433A CA2792433A CA2792433C CA 2792433 C CA2792433 C CA 2792433C CA 2792433 A CA2792433 A CA 2792433A CA 2792433 A CA2792433 A CA 2792433A CA 2792433 C CA2792433 C CA 2792433C
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sequence
target
pcr
nucleic acid
reaction mixture
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French (fr)
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CA2792433A1 (en
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Gerassimos Makrigiorgos
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Dana Farber Cancer Institute Inc
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Dana Farber Cancer Institute Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
CA2792433A 2010-03-08 2011-03-08 Full cold-pcr enrichment with reference blocking sequence Active CA2792433C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US31164210P 2010-03-08 2010-03-08
US61/311,642 2010-03-08
PCT/US2011/027473 WO2011112534A1 (en) 2010-03-08 2011-03-08 Full cold-pcr enrichment with reference blocking sequence

Publications (2)

Publication Number Publication Date
CA2792433A1 CA2792433A1 (en) 2011-09-15
CA2792433C true CA2792433C (en) 2016-10-11

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CA2792433A Active CA2792433C (en) 2010-03-08 2011-03-08 Full cold-pcr enrichment with reference blocking sequence

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US (3) US8623603B2 (enExample)
EP (1) EP2545189B1 (enExample)
JP (1) JP5795341B2 (enExample)
KR (2) KR101550489B1 (enExample)
CN (1) CN102859003B (enExample)
AU (1) AU2011224534B2 (enExample)
CA (1) CA2792433C (enExample)
DK (1) DK2545189T3 (enExample)
ES (1) ES2665500T3 (enExample)
WO (1) WO2011112534A1 (enExample)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP2545189B1 (en) 2010-03-08 2018-01-10 Dana-Farber Cancer Institute, Inc. Full cold-pcr enrichment with reference blocking sequence
EP3144396B1 (en) 2010-10-27 2020-01-01 President and Fellows of Harvard College Methods of use of toehold hairpin primer
JP2014507950A (ja) 2011-02-28 2014-04-03 トランスゲノミック，インク． 混合個体群における標的ｄｎａを配列決定するためのキットおよび方法
US11130992B2 (en) 2011-03-31 2021-09-28 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable multiplex COLD-PCR
WO2012151328A2 (en) 2011-05-02 2012-11-08 President And Fellows Of Harvard College Spatial sequestration of dynamic nucleic acid circuits
US9617392B2 (en) 2011-07-10 2017-04-11 President And Fellows Of Harvard College Compositions and methods for self-assembly of polymers with complementary macroscopic and microscopic scale units
CN103131756B (zh) * 2011-11-24 2014-08-27 百奥迈科生物技术有限公司 一种小核酸的检测方法及其应用
JP5846496B2 (ja) * 2012-04-19 2016-01-20 国立大学法人 鹿児島大学 ＬＮＡクランプ技術による植物内生菌のＳＳＵｒＲＮＡ遺伝子の選択的ＰＣＲ増幅法
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
US9279146B2 (en) 2012-12-21 2016-03-08 Roche Molecular Systems, Inc. Compounds and methods for the enrichment of mutated nucleic acid from a mixture
EP2948564B1 (en) 2013-01-23 2019-04-10 Brandeis University Reagents for improving pcr accuracy
US10913977B2 (en) 2013-07-24 2021-02-09 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR
KR20160064192A (ko) 2013-10-03 2016-06-07 얀센 바이오테크 인코포레이티드 프로톡신-ii 변이체 및 사용 방법
US20160115556A1 (en) * 2013-10-19 2016-04-28 Trovagene, Inc. Detecting mutations in disease over time
CA2926722A1 (en) * 2013-10-19 2015-04-23 Trovagene, Inc. Detecting mutations in disease over time
CA2922261A1 (en) * 2013-10-20 2015-05-21 Trovagene, Inc. Synthesis and enrichment of nucleic acid sequences
JP2016535987A (ja) * 2013-10-20 2016-11-24 トローバジーン インコーポレイテッド 核酸配列の合成及び濃縮
US9873908B2 (en) 2013-11-27 2018-01-23 Roche Molecular Systems, Inc. Methods for the enrichment of mutated nucleic acid from a mixture
CN103602748A (zh) * 2013-11-28 2014-02-26 瑞希基因科技（北京）股份有限公司 一种结合荧光定量pcr技术的焦磷酸测序方法
JP7016215B2 (ja) * 2014-01-27 2022-02-04 ザ ジェネラル ホスピタル コーポレイション 配列決定のために核酸を調製する方法
WO2015196154A1 (en) * 2014-06-20 2015-12-23 Richardson Katherine System, method, data module and kit for detecting variant nucleotide sequences
BR112017020896A2 (pt) 2015-04-02 2018-07-10 Janssen Biotech Inc variantes de protoxina-ii e métodos de uso
WO2016172265A1 (en) * 2015-04-20 2016-10-27 Neogenomics Laboratories, Inc. Method to increase sensitivity of next generation sequencing
JP6941568B2 (ja) 2015-06-24 2021-09-29 デイナ ファーバー キャンサー インスティチュート，インコーポレイテッド ヌクレアーゼを使用する野生型ｄｎａの選択的分解および突然変異体対立遺伝子の濃縮
CN105063208B (zh) * 2015-08-10 2018-03-06 北京吉因加科技有限公司 一种血浆中游离的目标dna低频突变富集测序方法
WO2017070339A1 (en) * 2015-10-20 2017-04-27 Richardson Katherine Microfluidic device for enrichment of nucleic acid sequence alterations
CN107338240B (zh) * 2015-11-25 2020-11-24 葛猛 对样品中目标核酸序列进行偏向扩增的方法和试剂盒
CN105821120B (zh) * 2016-02-03 2019-04-30 广州医科大学附属肿瘤医院 一种基于巢式复合cold-pcr的t790m突变检测方法
WO2018111835A1 (en) 2016-12-12 2018-06-21 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection
CA3059840C (en) * 2017-04-23 2022-04-26 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
ES2937929T3 (es) 2017-04-23 2023-04-03 Illumina Inc Composiciones y métodos para mejorar la identificación de muestras en bibliotecas de ácido nucleico indexadas
WO2018197945A1 (en) 2017-04-23 2018-11-01 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
AU2018260627B2 (en) 2017-04-23 2024-08-22 Illumina Cambridge Limited Compositions and methods for improving sample identification in indexed nucleic acid libraries
WO2019023243A1 (en) 2017-07-24 2019-01-31 Dana-Farber Cancer Institute, Inc. METHODS AND COMPOSITIONS FOR SELECTING AND AMPLIFYING DNA TARGETS IN A SINGLE REACTION MIXTURE
CN107858401A (zh) * 2017-10-31 2018-03-30 重庆邮电大学 一种低丰度基因突变的富集方法
CN120856897A (zh) 2019-02-02 2025-10-28 北京字节跳动网络技术有限公司 用于视频编解码中的帧内块复制的缓冲区更新
KR20240117647A (ko) 2019-02-02 2024-08-01 베이징 바이트댄스 네트워크 테크놀로지 컴퍼니, 리미티드 비디오 코딩에서 인트라 블록 복사를 위한 버퍼 관리
CN113545068B (zh) * 2019-03-01 2023-09-15 北京字节跳动网络技术有限公司 用于视频编解码中的帧内块复制的基于顺序的更新
EP3915265A4 (en) 2019-03-01 2022-06-22 Beijing Bytedance Network Technology Co., Ltd. DIRECTION-BASED PREDICTION FOR INTRA BLOCK COPY IN VIDEO CODING
CN117640927A (zh) 2019-03-04 2024-03-01 北京字节跳动网络技术有限公司 视频编解码中的帧内块复制中的实施方式方面
CA3146016C (en) 2019-07-06 2024-05-07 Beijing Bytedance Network Technology Co., Ltd. Virtual prediction buffer for intra block copy in video coding
KR102635519B1 (ko) 2019-07-10 2024-02-07 베이징 바이트댄스 네트워크 테크놀로지 컴퍼니, 리미티드 비디오 코딩에서 인트라 블록 카피를 위한 샘플 식별
AU2020334071A1 (en) 2019-08-21 2022-01-20 Dana-Farber Cancer Institute, Inc. Multi-site enrichment of deletions in DNA microsatellites
JP2024531713A (ja) * 2021-09-13 2024-08-29 ネクスジェン キャンサー ディテクション エルエルシー 試料中の標的ポリヌクレオチドを検出するための方法
WO2025079958A1 (ko) * 2023-10-11 2025-04-17 고려대학교 산학협력단 높은 처리량의 돌연변이 특이적 아답터 리게이션 방법

Family Cites Families (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4458066A (en) 1980-02-29 1984-07-03 University Patents, Inc. Process for preparing polynucleotides
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4800159A (en) 1986-02-07 1989-01-24 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences
US5310652A (en) 1986-08-22 1994-05-10 Hoffman-La Roche Inc. Reverse transcription with thermostable DNA polymerase-high temperature reverse transcription
US5541308A (en) 1986-11-24 1996-07-30 Gen-Probe Incorporated Nucleic acid probes for detection and/or quantitation of non-viral organisms
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
GB2228086A (en) 1988-11-25 1990-08-15 Ici Plc Characterisation of genomic DNA
KR920700360A (ko) 1989-03-22 1992-02-19 하리크 프리드리히 미끄럼 베어링
US5075217A (en) 1989-04-21 1991-12-24 Marshfield Clinic Length polymorphisms in (dC-dA)n ·(dG-dT)n sequences
AU5645690A (en) 1989-05-05 1990-11-29 Lifecodes Corporation Method for genetic analysis of a nucleic acid sample
US5256775A (en) * 1989-06-05 1993-10-26 Gilead Sciences, Inc. Exonuclease-resistant oligonucleotides
CA2020958C (en) 1989-07-11 2005-01-11 Daniel L. Kacian Nucleic acid sequence amplification methods
US5045450A (en) 1989-07-13 1991-09-03 Massachusetts Institute Of Technology Determination of a mutational spectrum
DE3938907C2 (de) 1989-11-24 1999-11-04 Dade Behring Marburg Gmbh Mittel zum Lagern und Suspendieren von Zellen, insbesondere Erythrozyten
US5427930A (en) 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
EP0439182B1 (en) 1990-01-26 1996-04-24 Abbott Laboratories Improved method of amplifying target nucleic acids applicable to both polymerase and ligase chain reactions
EP0516753A4 (en) 1990-02-26 1994-05-18 Us Health A method for the fluorescent detection of a dna sequence in real time
WO1991014003A2 (en) 1990-03-09 1991-09-19 Ig Laboratories, Inc. Characterization and analysis of polymorphic vntr loci
DE69220084T2 (de) 1990-05-07 1997-09-11 Daikin Ind Ltd Diagnostische Anwendungen der Bildung von doppelten D-Loops.
US5846710A (en) 1990-11-02 1998-12-08 St. Louis University Method for the detection of genetic diseases and gene sequence variations by single nucleotide primer extension
US5994056A (en) 1991-05-02 1999-11-30 Roche Molecular Systems, Inc. Homogeneous methods for nucleic acid amplification and detection
US5648211A (en) 1994-04-18 1997-07-15 Becton, Dickinson And Company Strand displacement amplification using thermophilic enzymes
GB2293238A (en) 1994-09-13 1996-03-20 Inceltec Ltd Primers for replication and/or amplification reactions
US5629178A (en) * 1994-10-28 1997-05-13 Genetics & Ivf Institute Method for enhancing amplification in the polymerase chain reaction employing peptide nucleic acid (PNA)
US5565340A (en) 1995-01-27 1996-10-15 Clontech Laboratories, Inc. Method for suppressing DNA fragment amplification during PCR
US5631147A (en) 1995-09-21 1997-05-20 Becton, Dickinson And Company Detection of nucleic acids in cells by thermophilic strand displacement amplification
US6143495A (en) 1995-11-21 2000-11-07 Yale University Unimolecular segment amplification and sequencing
US5612473A (en) 1996-01-16 1997-03-18 Gull Laboratories Methods, kits and solutions for preparing sample material for nucleic acid amplification
US6010878A (en) 1996-05-20 2000-01-04 Smithkline Beecham Corporation Interleukin-1 β converting enzyme like apoptotic protease-6
US6599704B2 (en) 1997-03-04 2003-07-29 Christoph Wagener Method for detecting mutated alleles
US5849497A (en) * 1997-04-03 1998-12-15 The Research Foundation Of State University Of New York Specific inhibition of the polymerase chain reaction using a non-extendable oligonucleotide blocker
JP3785517B2 (ja) 1997-06-05 2006-06-14 東ソー株式会社 核酸融解温度測定法
JP4236812B2 (ja) 1997-09-12 2009-03-11 エクシコン エ／エス オリゴヌクレオチド類似体
US6174680B1 (en) 1998-12-30 2001-01-16 Dana-Farber Cancer Institute, Inc. Method for identifying mismatch repair glycosylase reactive sites, compounds and uses thereof
FR2779154B1 (fr) 1998-05-27 2002-07-12 Bio Merieux Procede d'amplification d'au moins une sequence nucleotidique particuliere et amorces de mise en oeuvre
US6994962B1 (en) 1998-12-09 2006-02-07 Massachusetts Institute Of Technology Methods of identifying point mutations in a genome
US6850846B2 (en) 2000-01-11 2005-02-01 Affymetrix, Inc. Computer software for genotyping analysis using pattern recognition
DE10012540B4 (de) 2000-03-15 2004-09-23 Vermicon Ag Oligonukleotide und Verfahren zum spezifischen Nachweis von Mikroorganismen durch Polymerase-Kettenreaktion
JP2004520812A (ja) 2000-08-30 2004-07-15 ハプロゲン・エルエルシー 対立遺伝子を決定するための方法
EP1253205A1 (en) 2001-04-24 2002-10-30 LION Bioscience AG Method of blocking amplification of selected sequences
US6762049B2 (en) 2001-07-05 2004-07-13 Institute Of Microelectronics Miniaturized multi-chamber thermal cycler for independent thermal multiplexing
DE10158516A1 (de) 2001-11-29 2003-06-12 Focusgenomics Gmbh Verfahren zum Nachweis von Hybridisierungsereignissen in Nukleinsäuren
AUPS076802A0 (en) 2002-02-26 2002-03-21 Commonwealth Scientific And Industrial Research Organisation Selective nucleic acid amplification
AUPS076902A0 (en) 2002-02-26 2002-03-21 Commonwealth Scientific And Industrial Research Organisation Novel selective polymerase chain reaction
AU2003231121B2 (en) 2002-04-26 2008-08-07 University Of Utah Research Foundation Characterization of single stranded nucleic acids by melting analysis using a double strand specific nucleic acid dye
US20040022764A1 (en) * 2002-07-31 2004-02-05 Hanan Polansky Inhibition of microcompetition with a foreign polynucleotide as treatment of chronic disease
WO2004055193A1 (en) 2002-12-18 2004-07-01 Dingbang Xu Pcr method and application by transnormal low thermo-denaturation temperature
WO2004058990A2 (en) * 2002-12-20 2004-07-15 Applera Corporation Genetic polymorphisms associated with stenosis, methods of detection and uses thereof
GB0406863D0 (en) 2004-03-26 2004-04-28 Qiagen As Nucleic acid sequencing
US7749707B2 (en) 2005-03-01 2010-07-06 Wako Pure Chemical Industries, Ltd. Method for obtaining subtraction polynucleotide
US7456281B2 (en) * 2005-04-20 2008-11-25 Idaho Technology, Inc. Nucleic acid melting analysis with saturation dyes
US8076082B2 (en) 2005-10-14 2011-12-13 Cleveland State University Methods for identifying multiple DNA alteration markers in a large background of wild-type DNA
US20070154892A1 (en) 2005-12-30 2007-07-05 Simon Wain-Hobson Differential amplification of mutant nucleic acids by PCR in a mixure of nucleic acids
WO2007087310A2 (en) 2006-01-23 2007-08-02 Population Genetics Technologies Ltd. Nucleic acid analysis using sequence tokens
US20100009355A1 (en) * 2006-03-14 2010-01-14 Kolodney Michael S Selective amplification of minority mutations using primer blocking high-affinity oligonucleotides
US8501403B2 (en) 2006-03-28 2013-08-06 Commonwealth Scientific And Industrial Research Organisation Amplification of DNA fragments
US20080269068A1 (en) 2007-02-06 2008-10-30 President And Fellows Of Harvard College Multiplex decoding of sequence tags in barcodes
US7635566B2 (en) 2007-06-29 2009-12-22 Population Genetics Technologies Ltd. Methods and compositions for isolating nucleic acid sequence variants
CA2695264C (en) 2007-08-01 2015-01-27 Dana-Farber Cancer Institute Enrichment of a target sequence
US8071338B2 (en) * 2007-08-08 2011-12-06 Roche Molecular Systems, Inc. Suppression of amplification using an oligonucleotide and a polymerase significantly lacking 5′-3′ nuclease activity
WO2010065626A1 (en) 2008-12-02 2010-06-10 Globeimmune, Inc. Genotyping tools, methods and kits
US8206929B2 (en) * 2009-01-07 2012-06-26 Roche Molecular Systems, Inc. Nucleic acid amplification with allele-specific suppression of sequence variants
EP3018218B1 (en) 2009-07-21 2018-01-24 Gen-Probe Incorporated Methods for detection of nucleic acid sequences over an extended dynamic range
EP2545189B1 (en) 2010-03-08 2018-01-10 Dana-Farber Cancer Institute, Inc. Full cold-pcr enrichment with reference blocking sequence
JP2014507950A (ja) 2011-02-28 2014-04-03 トランスゲノミック，インク． 混合個体群における標的ｄｎａを配列決定するためのキットおよび方法
US9260753B2 (en) 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
US11130992B2 (en) 2011-03-31 2021-09-28 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable multiplex COLD-PCR
JP6040227B2 (ja) 2011-05-19 2016-12-07 アジーナ バイオサイエンス， インコーポレイテッド マルチプレックス核酸同定のための製品およびプロセス
US9238836B2 (en) 2012-03-30 2016-01-19 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing modified nucleic acids
US9133490B2 (en) 2012-05-16 2015-09-15 Transgenomic, Inc. Step-up method for COLD-PCR enrichment
KR102213886B1 (ko) 2013-03-14 2021-02-05 다카라 바이오 가부시키가이샤 내열성 미스매치 엔도뉴클레아제의 이용방법
US9797001B2 (en) 2013-04-17 2017-10-24 Pioneer Hi-Bred International, Inc. Methods for characterizing a target DNA sequence composition in a plant genome
US10913977B2 (en) 2013-07-24 2021-02-09 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable enrichment of minor DNA alleles by limiting denaturation time in PCR or simply enable enrichment of minor DNA alleles by limiting the denaturation time in PCR
US9873908B2 (en) 2013-11-27 2018-01-23 Roche Molecular Systems, Inc. Methods for the enrichment of mutated nucleic acid from a mixture
WO2018111835A1 (en) 2016-12-12 2018-06-21 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of dna molecules prior to mutation enrichment and/or mutation detection

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Publication number Publication date
CN102859003A (zh) 2013-01-02
JP2013521776A (ja) 2013-06-13
DK2545189T3 (en) 2018-04-23
US8623603B2 (en) 2014-01-07
US20110217714A1 (en) 2011-09-08
CN102859003B (zh) 2014-07-30
KR20150067398A (ko) 2015-06-17
US20140106362A1 (en) 2014-04-17
ES2665500T3 (es) 2018-04-26
US9957556B2 (en) 2018-05-01
CA2792433A1 (en) 2011-09-15
JP5795341B2 (ja) 2015-10-14
EP2545189A1 (en) 2013-01-16
US20180282798A1 (en) 2018-10-04
AU2011224534A1 (en) 2012-09-27
AU2011224534B2 (en) 2014-10-09
US11174510B2 (en) 2021-11-16
KR20120139780A (ko) 2012-12-27
EP2545189B1 (en) 2018-01-10
KR101550489B1 (ko) 2015-09-07
WO2011112534A1 (en) 2011-09-15

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