AU2011224534B2 - Full COLD-PCR enrichment with reference blocking sequence - Google Patents

Full COLD-PCR enrichment with reference blocking sequence Download PDF

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Publication number
AU2011224534B2
AU2011224534B2 AU2011224534A AU2011224534A AU2011224534B2 AU 2011224534 B2 AU2011224534 B2 AU 2011224534B2 AU 2011224534 A AU2011224534 A AU 2011224534A AU 2011224534 A AU2011224534 A AU 2011224534A AU 2011224534 B2 AU2011224534 B2 AU 2011224534B2
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Australia
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sequence
nucleic acid
target
strands
pcr
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AU2011224534A1 (en
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Gerassimos Makrigiorgos
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Dana Farber Cancer Institute Inc
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Dana Farber Cancer Institute Inc
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/107—Temperature of melting, i.e. Tm

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
AU2011224534A 2010-03-08 2011-03-08 Full COLD-PCR enrichment with reference blocking sequence Ceased AU2011224534B2 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US31164210P 2010-03-08 2010-03-08
US61/311,642 2010-03-08
PCT/US2011/027473 WO2011112534A1 (en) 2010-03-08 2011-03-08 Full cold-pcr enrichment with reference blocking sequence

Publications (2)

Publication Number Publication Date
AU2011224534A1 AU2011224534A1 (en) 2012-09-27
AU2011224534B2 true AU2011224534B2 (en) 2014-10-09

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AU2011224534A Ceased AU2011224534B2 (en) 2010-03-08 2011-03-08 Full COLD-PCR enrichment with reference blocking sequence

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US (3) US8623603B2 (enExample)
EP (1) EP2545189B1 (enExample)
JP (1) JP5795341B2 (enExample)
KR (2) KR101550489B1 (enExample)
CN (1) CN102859003B (enExample)
AU (1) AU2011224534B2 (enExample)
CA (1) CA2792433C (enExample)
DK (1) DK2545189T3 (enExample)
ES (1) ES2665500T3 (enExample)
WO (1) WO2011112534A1 (enExample)

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AU2011224534A1 (en) 2012-09-27
JP5795341B2 (ja) 2015-10-14
CA2792433C (en) 2016-10-11
US8623603B2 (en) 2014-01-07
KR20120139780A (ko) 2012-12-27
US20110217714A1 (en) 2011-09-08
ES2665500T3 (es) 2018-04-26
CA2792433A1 (en) 2011-09-15
CN102859003B (zh) 2014-07-30
KR101550489B1 (ko) 2015-09-07
CN102859003A (zh) 2013-01-02
US20140106362A1 (en) 2014-04-17
JP2013521776A (ja) 2013-06-13
US20180282798A1 (en) 2018-10-04
US11174510B2 (en) 2021-11-16
WO2011112534A1 (en) 2011-09-15
EP2545189A1 (en) 2013-01-16
DK2545189T3 (en) 2018-04-23
KR20150067398A (ko) 2015-06-17
EP2545189B1 (en) 2018-01-10
US9957556B2 (en) 2018-05-01

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