CA2774776A1 - Novel glycosyl hydrolase enzymes and uses thereof - Google Patents

Novel glycosyl hydrolase enzymes and uses thereof Download PDF

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Publication number
CA2774776A1
CA2774776A1 CA2774776A CA2774776A CA2774776A1 CA 2774776 A1 CA2774776 A1 CA 2774776A1 CA 2774776 A CA2774776 A CA 2774776A CA 2774776 A CA2774776 A CA 2774776A CA 2774776 A1 CA2774776 A1 CA 2774776A1
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Canada
Prior art keywords
seq
polypeptide
composition
amino acid
residues
Prior art date
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Abandoned
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CA2774776A
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English (en)
French (fr)
Inventor
Benjamin Bower
Megan Yee Hsi
Thijs Kaper
Bradley R. Kelemen
Suzanne E. Lantz
Edmund A. Larenas
Colin Mitchinson
Steven Kim
William D. Hitz
Mark Emptage
Keith Dumont Wing
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Danisco US Inc
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Danisco US Inc
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Application filed by Danisco US Inc filed Critical Danisco US Inc
Publication of CA2774776A1 publication Critical patent/CA2774776A1/en
Abandoned legal-status Critical Current

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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0065—Oxidoreductases (1.) acting on hydrogen peroxide as acceptor (1.11)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2445—Beta-glucosidase (3.2.1.21)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • C12N9/2485—Xylan endo-1,3-beta-xylosidase (3.2.1.32), i.e. endo-1,3-beta-xylanase
    • C—CHEMISTRY; METALLURGY
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    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
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    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/08—Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
    • C—CHEMISTRY; METALLURGY
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    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01037—Xylan 1,4-beta-xylosidase (3.2.1.37)
    • C—CHEMISTRY; METALLURGY
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    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01055—Alpha-N-arabinofuranosidase (3.2.1.55)
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    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
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    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01021—Beta-glucosidase (3.2.1.21)
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

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  • Chemical & Material Sciences (AREA)
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  • Bioinformatics & Cheminformatics (AREA)
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  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
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  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2774776A 2009-09-23 2010-09-22 Novel glycosyl hydrolase enzymes and uses thereof Abandoned CA2774776A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US24527309P 2009-09-23 2009-09-23
US61/245,273 2009-09-23
US28988609P 2009-12-23 2009-12-23
US61/289,886 2009-12-23
PCT/US2010/049849 WO2011038019A2 (en) 2009-09-23 2010-09-22 Novel glycosyl hydrolase enzymes and uses thereof

Publications (1)

Publication Number Publication Date
CA2774776A1 true CA2774776A1 (en) 2011-03-31

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ID=43796468

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2774776A Abandoned CA2774776A1 (en) 2009-09-23 2010-09-22 Novel glycosyl hydrolase enzymes and uses thereof

Country Status (16)

Country Link
US (3) US20130177947A1 (cg-RX-API-DMAC7.html)
EP (2) EP3296394B1 (cg-RX-API-DMAC7.html)
JP (2) JP5932648B2 (cg-RX-API-DMAC7.html)
KR (1) KR20120115206A (cg-RX-API-DMAC7.html)
CN (1) CN102791853B (cg-RX-API-DMAC7.html)
AU (3) AU2010298308A1 (cg-RX-API-DMAC7.html)
BR (2) BR122018015957B1 (cg-RX-API-DMAC7.html)
CA (1) CA2774776A1 (cg-RX-API-DMAC7.html)
CO (1) CO6630115A2 (cg-RX-API-DMAC7.html)
DK (2) DK2480660T5 (cg-RX-API-DMAC7.html)
IN (1) IN2012DN01492A (cg-RX-API-DMAC7.html)
MX (2) MX353237B (cg-RX-API-DMAC7.html)
RU (1) RU2012116081A (cg-RX-API-DMAC7.html)
UA (1) UA115219C2 (cg-RX-API-DMAC7.html)
WO (1) WO2011038019A2 (cg-RX-API-DMAC7.html)
ZA (1) ZA201201147B (cg-RX-API-DMAC7.html)

Families Citing this family (51)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
MX353237B (es) 2009-09-23 2018-01-08 Danisco Us Inc Enzimas de glicosil hidrolasa novedosas y usos de las mismas.
US10138499B2 (en) * 2009-12-23 2018-11-27 Danisco Us Inc. Methods for improving the efficiency of simultaneous saccharification and fermentation reactions
US9701969B2 (en) 2010-06-03 2017-07-11 Danisco Us Inc. Filamentous fungal host strains and DNA constructs, and methods of use thereof
CA2808777C (en) 2010-08-25 2019-10-29 Danisco Us Inc. Filamentous fungi having an altered viscosity phenotype
MX2013010512A (es) 2011-03-17 2013-10-07 Danisco Inc Metodo para reducir la viscosidad en un proceso de sacarificacion.
WO2012125937A2 (en) * 2011-03-17 2012-09-20 Danisco Us Inc. Glycosyl hydrolase enzymes and uses thereof for biomass hydrolysis
BR112014008493A2 (pt) * 2011-11-11 2019-09-24 Novozymes Inc polipeptídeo isolado, composição, polinucleotídeo isolado, célula hospedeira recombinante, métodos para produção de um polipeptídeo, de um mutante ou célula de origem, e de uma proteína, planta transgênica, parte da planta ou célula vegetal, molécula de rna inibidora de fita dupla, método para inibir a expressão de um polipeptídeo, processos para degradar um material celulósico ou contendo xilano, para produzir um produto de fermentação, e para fermentação de um material celulósico ou contendo xilano, e, formulação de caldo completo ou composição de cultura de células
DK2785732T3 (en) 2011-12-01 2017-06-19 Novozymes Inc POLYPEPTIDES WITH BETA-XYLOSIDASE ACTIVITY AND POLYNUCLEOTIDES CODING THEM
BR112014031024A2 (pt) 2012-06-11 2017-06-27 Codexis Inc xilanases e xilosidases fúngicas.
KR20150038584A (ko) * 2012-08-03 2015-04-08 듀폰 뉴트리션 바이오사이언시즈 에이피에스 옥수수 및 옥수수 부산물에 있어서 자일라나아제의 용도
CN104781385A (zh) 2012-09-20 2015-07-15 丹尼斯科美国公司 用于对细胞培养物控释培养组分的微量滴定板
WO2014049138A1 (en) * 2012-09-28 2014-04-03 Dsm Ip Assets B.V. Process for the digestion of organic material
CA2889193A1 (en) 2012-10-31 2014-05-08 Danisco Us Inc. Beta-glucosidase from neurospora crassa
CA2888757A1 (en) 2012-10-31 2014-05-08 Danisco Us Inc. Compositions and methods of use
CN104781399A (zh) 2012-10-31 2015-07-15 丹尼斯科美国公司 来自稻瘟病菌的β-葡糖苷酶
CA2892054A1 (en) * 2012-12-07 2014-06-12 Ling Hua Compositions and methods of use
MX2015007234A (es) 2012-12-12 2015-10-29 Danisco Us Inc Variantes de celobiohidrolasas.
US8846357B2 (en) 2012-12-20 2014-09-30 E I Du Pont De Nemours And Company Stabilized chlorine dioxide for contamination control in Zymomonas fermentation
CN103146725B (zh) * 2013-02-07 2014-07-02 广西大学 热稳定β-葡萄糖苷酶基因及其应用
US8759040B1 (en) * 2013-02-12 2014-06-24 Novozymes Inc. Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
US20140273105A1 (en) 2013-03-12 2014-09-18 E I Du Pont De Nemours And Company Gradient pretreatment of lignocellulosic biomass
WO2015017255A1 (en) 2013-07-29 2015-02-05 Danisco Us Inc. Variant enzymes
US20150087041A1 (en) 2013-09-26 2015-03-26 E I Du Pont De Nemours And Company Production of ethanol with reduced contaminants in a cellulosic biomass based process
US20150087040A1 (en) 2013-09-26 2015-03-26 E I Du Pont De Nemours And Company Production of ethanol and recycle water in a cellulosic fermentation process
US9873846B2 (en) 2013-10-10 2018-01-23 E I Du Pont De Nemours And Company Fuel compositions containing lignocellulosic biomass fermentation process syrup
US9725363B2 (en) 2013-10-10 2017-08-08 E I Du Pont De Nemours And Company Lignocellulosic biomass fermentation process co-product fuel for cement kiln
US20150101751A1 (en) 2013-10-10 2015-04-16 E I Du Pont De Nemours And Company Lignocellulosic biomass fermentation process syrup binder and adhesive
US20150147786A1 (en) 2013-11-24 2015-05-28 E I Du Pont De Nemours And Company High force and high stress destructuring for starch biomass processing
JP2015136324A (ja) * 2014-01-22 2015-07-30 本田技研工業株式会社 糖化酵素組成物
GB201401680D0 (en) * 2014-01-31 2014-03-19 Dupont Nutrition Biosci Aps Compositions and methods comprising a xylanase enzyme variant
SG11201701961YA (en) * 2014-09-26 2017-04-27 Xyleco Inc Solubilized enzyme and uses thereof
JP6346853B2 (ja) * 2014-12-04 2018-06-20 本田技研工業株式会社 麹菌変異株、形質転換体及び糖化酵素の生産方法
MX2017016625A (es) 2015-07-07 2018-05-15 Danisco Us Inc Induccion de la expresion genica usando una mezcla de azucar de alta concentracion.
WO2017103159A2 (en) * 2015-12-18 2017-06-22 Novozymes A/S Polypeptides having xylanase activity and polynucleotides encoding same
EP3974527A1 (en) * 2016-03-31 2022-03-30 Toray Industries, Inc. Description method for producing protein
WO2018053058A1 (en) 2016-09-14 2018-03-22 Danisco Us Inc. Lignocellulosic biomass fermentation-based processes
KR102593668B1 (ko) 2016-10-04 2023-10-24 다니스코 유에스 인크. 유도 기질 부재 하의 사상 진균 세포에서의 단백질 생산
WO2018106656A1 (en) 2016-12-06 2018-06-14 Danisco Us Inc Truncated lpmo enzymes and use thereof
US20190330577A1 (en) 2016-12-21 2019-10-31 Dupont Nutrition Biosciences Aps Methods of using thermostable serine proteases
US20200109354A1 (en) * 2017-04-04 2020-04-09 Novozymes A/S Polypeptides
US11407964B2 (en) 2017-04-06 2022-08-09 Novozymes A/S Cleaning compositions and uses thereof
WO2019074828A1 (en) 2017-10-09 2019-04-18 Danisco Us Inc CELLOBIOSE DEHYDROGENASE VARIANTS AND METHODS OF USE
CN108342400B (zh) * 2018-03-05 2021-04-06 武汉康复得生物科技股份有限公司 重组表达草酸氧化酶的基因工程菌及其构建方法和应用
US11913046B2 (en) 2018-04-23 2024-02-27 Inbiose N.V. Increasing export of 2'fucosyllactose from microbial cells through the expression of a heterologous nucleic acid
US12071614B2 (en) 2018-04-23 2024-08-27 Inbiose N.V. Increasing activity of 2′fucosyllactose transporters endogenous to microbial cells
CN109370995B (zh) * 2018-12-11 2021-05-25 中国农业科学院北京畜牧兽医研究所 锰过氧化物酶CsMnP及其编码基因和应用
CN109957514A (zh) * 2019-01-25 2019-07-02 南昌大学 一种粗壮脉纹孢菌的新用途
US12232512B2 (en) * 2019-06-05 2025-02-25 Danisco Us Inc. Methods for improving the amino acid content of animal feed products
CN110862976B (zh) * 2019-12-11 2021-04-27 云南师范大学 一种盐耐受性改良的木糖苷酶突变体k321dh328d及其应用
WO2024137389A1 (en) * 2022-12-20 2024-06-27 International N&H Denmark Aps Use of a fungal protein comprising an over expressed native polypeptide to provide a plant-based meat alternative
CN119752849B (zh) * 2024-11-19 2025-09-23 安徽医科大学 一种酶学性质提高的木聚糖酶突变体及其应用

Family Cites Families (135)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5366558A (en) 1979-03-23 1994-11-22 Brink David L Method of treating biomass material
US4797361A (en) 1983-10-24 1989-01-10 Lehigh University Microorganism and process
CA1333777C (en) 1988-07-01 1995-01-03 Randy M. Berka Aspartic proteinase deficient filamentous fungi
US5536655A (en) 1989-09-26 1996-07-16 Midwest Research Institute Gene coding for the E1 endoglucanase
DK16490D0 (da) 1990-01-19 1990-01-19 Novo Nordisk As Enzym
US5326477A (en) 1990-05-07 1994-07-05 Bio-Sep, Inc. Process for digesting solid waste
DE69107455T3 (de) 1990-05-09 2004-09-23 Novozymes A/S Eine ein endoglucanase enzym enthaltende zellulasezubereitung.
DK115890D0 (da) 1990-05-09 1990-05-09 Novo Nordisk As Enzym
US6103464A (en) 1990-12-10 2000-08-15 Genencor International, Inc. Method of detecting DNA encoding a β-glucosidase from a filamentous fungus
DK0562003T4 (en) 1990-12-10 2015-07-13 Danisco Us Inc Improved saccharification of cellulose by cloning and amplification of.-Glucosidase gene from Tricodermareesei
ATE166386T1 (de) 1991-03-18 1998-06-15 Gist Brocades Nv Klonierung und expression einer acetylxylanesterase aus fungi
US6021536A (en) 1993-03-04 2000-02-08 Wasinger; Eric Mechanical desizing and abrading device
US5405769A (en) 1993-04-08 1995-04-11 National Research Council Of Canada Construction of thermostable mutants of a low molecular mass xylanase
DK81293D0 (da) 1993-07-06 1993-07-06 Novo Nordisk As Enzym
DK83993D0 (cg-RX-API-DMAC7.html) 1993-07-13 1993-07-13 Novo Nordisk As
US5437992A (en) 1994-04-28 1995-08-01 Genencor International, Inc. Five thermostable xylanases from microtetraspora flexuosa for use in delignification and/or bleaching of pulp
US6099844A (en) 1994-08-22 2000-08-08 Triarco Industries, Inc. Increasing yield of extractable substances from botanicals with an enzyme composition
US6426200B1 (en) 1994-09-15 2002-07-30 University Of Georgia Research Foundation, Inc. Methods for enzymatic deinking of waste paper
US5705369A (en) 1994-12-27 1998-01-06 Midwest Research Institute Prehydrolysis of lignocellulose
ES2169219T5 (es) 1995-01-26 2009-09-01 Novozymes A/S Aditivos alimenticios para animales que comprenden xilanasa.
CN102080070B (zh) 1995-03-17 2016-01-20 诺沃奇梅兹有限公司 新的内切葡聚糖酶
WO1997000964A1 (en) 1995-06-23 1997-01-09 Danisco Ingredients A/S (Danisco A/S) Novel beta-xylosidase, nucleotide sequence encoding it, and use thereof
WO1997004160A1 (en) 1995-07-19 1997-02-06 Novo Nordisk A/S Treatment of fabrics
EP1021513B1 (en) 1995-11-15 2004-02-18 Novozymes A/S A process for combined desizing and "stone-washing" of dyed denim
AU1438397A (en) 1996-01-29 1997-08-22 Novo Nordisk A/S Process for desizing cellulosic fabric
US6254722B1 (en) 1996-03-27 2001-07-03 North Carolina State University Method for making dissolving pulp from paper products containing hardwood fibers
US5811381A (en) 1996-10-10 1998-09-22 Mark A. Emalfarb Cellulase compositions and methods of use
US7883872B2 (en) 1996-10-10 2011-02-08 Dyadic International (Usa), Inc. Construction of highly efficient cellulase compositions for enzymatic hydrolysis of cellulose
US6069122A (en) 1997-06-16 2000-05-30 The Procter & Gamble Company Dishwashing detergent compositions containing organic diamines for improved grease cleaning, sudsing, low temperature stability and dissolution
EP0988402A1 (en) 1997-06-10 2000-03-29 Xyrofin Oy Process for the production of sylose from a paper-grade, hardwood pulp
BR9810701A (pt) 1997-07-11 2000-08-08 Genencor Int Proteìna "swollenina" de trichoderma reesei e seqâências de codificação de dna
US6413928B1 (en) 1997-11-10 2002-07-02 The Procter & Gamble Company Process for preparing a detergent tablet
US6187580B1 (en) 1997-11-24 2001-02-13 Novo Nordisk A/S Pectate lyases
WO1999057360A1 (en) 1998-05-01 1999-11-11 Novo Nordisk A/S Enhancers such as n-hydroxyacetanilide
DE19824705A1 (de) 1998-06-03 1999-12-09 Henkel Kgaa Amylase und Protease enthaltende Wasch- und Reinigungsmittel
US5980581A (en) 1998-09-08 1999-11-09 The Virkler Company Process for desizing and cleaning woven fabrics and garments
US20020084046A1 (en) 1998-09-29 2002-07-04 Jay Chiehlung Hsu Enzymatic paper and process of making thereof
KR100618495B1 (ko) 1998-10-06 2006-08-31 마크 아론 에말파브 섬유상의 진균성 숙주 영역에서의 형질전환 시스템:크리소스포륨속에서
US6024766A (en) 1999-01-27 2000-02-15 Wasinger; Eric M. Process for enzymatic desizing of garments and enzyme deactivation
US6409841B1 (en) 1999-11-02 2002-06-25 Waste Energy Integrated Systems, Llc. Process for the production of organic products from diverse biomass sources
US6248535B1 (en) 1999-12-20 2001-06-19 University Of Southern California Method for isolation of RNA from formalin-fixed paraffin-embedded tissue specimens
DK1242601T3 (da) 1999-12-30 2005-06-13 Genencor Int Trichoderma reesei-xylanase
US7354755B2 (en) 2000-05-01 2008-04-08 Midwest Research Institute Stable zymomonas mobilis xylose and arabinose fermenting strains
US6423145B1 (en) 2000-08-09 2002-07-23 Midwest Research Institute Dilute acid/metal salt hydrolysis of lignocellulosics
DE10043662A1 (de) 2000-08-30 2001-02-22 Saechsisches Inst Fuer Angewan Verfahren zur enzymatischen Aktivierung von lignocellulosen Faserstoffen zur Herstellung von Werkstoffen
CN1509330A (zh) 2001-05-18 2004-06-30 ŵ��ø�ɷ����޹�˾ 具有纤维二糖酶活性的多肽和编码其的多核苷酸
US6982159B2 (en) 2001-09-21 2006-01-03 Genencor International, Inc. Trichoderma β-glucosidase
US7045331B2 (en) 2001-12-18 2006-05-16 Genencor International, Inc. EGVII endoglucanase and nucleic acids encoding the same
US7049125B2 (en) 2001-12-18 2006-05-23 Genencor International, Inc. EGVIII endoglucanase and nucleic acids encoding the same
US7056721B2 (en) 2001-12-18 2006-06-06 Genencor International, Inc. EGVI endoglucanase and nucleic acids encoding the same
US7045332B2 (en) 2001-12-18 2006-05-16 Genencor International, Inc. BGL4 β-glucosidase and nucleic acids encoding the same
US7005289B2 (en) 2001-12-18 2006-02-28 Genencor International, Inc. BGL5 β-glucosidase and nucleic acids encoding the same
US20040005674A1 (en) 2002-04-30 2004-01-08 Athenix Corporation Methods for enzymatic hydrolysis of lignocellulose
EP2305801B1 (en) 2002-08-16 2017-07-05 Danisco US Inc. Novel variant hyprocrea jecorina CBH1 cellulases
DK1545217T3 (da) 2002-09-10 2013-06-03 Genencor Int Induktion af genekspression ved anvendelse af en højkoncentrationssukkerblanding
US7371558B2 (en) 2002-10-04 2008-05-13 E.I. Du Pont De Nemours And Company Process for the biological production of 1,3-propanediol with high yield
DK1556512T3 (en) 2002-11-07 2016-09-12 Danisco Us Inc BGL6 BETA-GLUCOSIDASE AND NUCLEIC ACIDS THEREOF CODED
US7407788B2 (en) 2002-11-21 2008-08-05 Danisco A/S, Genencor Division BGL7 beta-glucosidase and nucleic acids encoding the same
US7449550B2 (en) 2003-02-27 2008-11-11 Alliance For Sustainable Energy, Llc Superactive cellulase formulation using cellobiohydrolase-1 from Penicillium funiculosum
US20040231060A1 (en) 2003-03-07 2004-11-25 Athenix Corporation Methods to enhance the activity of lignocellulose-degrading enzymes
JP2007534294A (ja) 2003-03-21 2007-11-29 ジェネンコー・インターナショナル・インク Ｃｂｈ１相同体及び変異体ｃｂｈ１セルラーゼ
SI1627050T1 (sl) 2003-04-01 2014-01-31 Danisco Us Inc. Varianta humicola grisea cbh1.1
CA2525333C (en) 2003-05-29 2016-03-01 Genencor International, Inc. Trichoderma genes
CN101415823B (zh) 2003-09-15 2012-10-17 金克克国际有限公司 修饰酶、生产修饰酶的方法及其用途
CN1902315B (zh) 2003-12-03 2012-05-02 明治制果药业株式会社 内切葡聚糖酶stce和含有内切葡聚糖酶的纤维素酶配制品
CN104726431B (zh) 2004-01-30 2018-06-01 诺维信股份有限公司 具有分解纤维增强活性的多肽及其编码多核苷酸
DK1733033T3 (da) 2004-02-06 2012-09-24 Novozymes Inc Polypeptider med cellulolytisk forbedrende aktivitet og polynukleotider, der koder for disse
US8097445B2 (en) 2004-03-25 2012-01-17 Danisco Us Inc. Exo-endo cellulase fusion protein
WO2005093050A2 (en) 2004-03-25 2005-10-06 Genencor International, Inc. Cellulase fusion protein and heterologous cellulase fusion construct encoding the same
DK1735454T3 (en) 2004-03-25 2017-08-28 Novozymes Inc PROCEDURES FOR DEVELOPMENT OR CONVERSION OF PLANT CELL CELL POLICY ACCHARIDES
EP1751281A2 (en) 2004-05-27 2007-02-14 Genencor International, Inc. Aspergillus kawachi acid-stable alpha amylase and applications in granular starch hydrolysis
WO2005118769A1 (en) 2004-06-03 2005-12-15 Novozymes A/S Mashing process and enzyme composition useful therein
US20060075522A1 (en) 2004-07-31 2006-04-06 Jaclyn Cleveland Genes and uses for plant improvement
CN101094917B (zh) 2004-12-30 2012-04-25 金克克国际有限公司 新型变体红褐肉座菌cbh2纤维素酶
US7781191B2 (en) 2005-04-12 2010-08-24 E. I. Du Pont De Nemours And Company Treatment of biomass to obtain a target chemical
US7998713B2 (en) 2005-04-12 2011-08-16 E. I. Du Pont De Nemours And Company Treatment of biomass to obtain ethanol
EP1877568B9 (en) 2005-04-26 2021-05-12 Novozymes A/S Hydrolysis of arabinoxylan
CN104404106A (zh) 2005-09-30 2015-03-11 诺维信股份有限公司 用于增强纤维素材料降解或转化的方法
US7256032B2 (en) 2005-12-22 2007-08-14 Ab Enzymes Oy Enzymes
FI120045B (fi) 2005-12-22 2009-06-15 Roal Oy Selluloosamateriaalin käsittely ja siinä käyttökelpoiset entsyymit
NZ595499A (en) 2006-02-10 2013-05-31 Verenium Corp Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
US8304212B2 (en) 2006-07-10 2012-11-06 Dyadic International, Inc. Methods and compositions for degradation of lignocellulosic material
US8017373B2 (en) 2006-08-31 2011-09-13 Iogen Energy Corporation Process for enzymatic hydrolysis of pretreated lignocellulosic feedstocks
WO2008039370A1 (en) 2006-09-22 2008-04-03 Danisco Us, Inc., Genencor Division Acetolactate synthase (als) selectable marker from trichoderma reesei
US7993884B2 (en) 2006-10-10 2011-08-09 The United States Of America As Represented By The Secretary Of Agriculture Beta-xylosidase for conversion of plant cell wall carbohydrates to simple sugars
PL2082054T5 (pl) 2006-11-13 2019-03-29 Danisco Inc Sposób poprawy uzysku procesów konwersji celulozy
DK2069491T3 (en) 2007-05-10 2018-04-09 Novozymes Inc COMPOSITIONS AND PROCEDURES FOR IMPROVING THE DECOMPOSITION OR CONVERSION OF CELLULOSE SUBSTANCES
WO2008153712A2 (en) 2007-05-21 2008-12-18 Danisco Us, Inc., Genencor Division Method for introducing nucleic acids into fungal cells
MX2009012844A (es) 2007-05-31 2009-12-15 Novozymes Inc Composiciones para degradar material celulosico.
US9453213B2 (en) 2007-05-31 2016-09-27 Novozymes, Inc. Methods of increasing the cellulolytic enhancing activity of a polypeptide
EA201300048A1 (ru) 2007-06-08 2013-09-30 ДАНИСКО ЮЭс, ИНК., ДЖЕНЕНКОР ДИВИЖН Система экспрессии гетерологичных и гомологичных целлюлаз
NZ582843A (en) 2007-06-27 2012-03-30 Novozymes As Methods for producing fermentation products
US20110265221A1 (en) 2007-07-10 2011-10-27 Monsanto Technology Llc Transgenic plants with enhanced agronomic traits
US7819976B2 (en) * 2007-08-22 2010-10-26 E. I. Du Pont De Nemours And Company Biomass treatment method
CA2698765A1 (en) 2007-09-07 2009-03-19 Danisco Us Inc. Beta-glucosidase enhanced filamentous fungal whole cellulase compositions and methods of use
WO2009033071A2 (en) * 2007-09-07 2009-03-12 Dyadic International, Inc. Novel fungal enzymes
MX2010003600A (es) * 2007-10-03 2010-12-14 Verenium Corp Xilanasas, acidos nucleicos que las codifican, y metodos para hacerlas y usarlas.
WO2009049067A1 (en) 2007-10-12 2009-04-16 Danisco Us Inc., Genencor Division Methods and compositions for enhanced production of organic sustances from fermenting microorganisms
WO2009074685A1 (en) 2007-12-12 2009-06-18 Novozymes A/S Enzymatic degradation of biomass substrates comprising mannan
AU2008334945C1 (en) 2007-12-13 2013-09-05 Danisco Us Inc. Compositions and methods for producing isoprene
US20100306881A1 (en) 2007-12-19 2010-12-02 Novozymes A/S Polypeptides having Cellulolytic Enhancing Activity and Polynucleotides Encoding Same
BRPI0908879B1 (pt) 2008-02-29 2019-07-09 University Of Central Florida Research Foundation, Inc. Método de degradação de planta, coquetel de degradação de plantas, material de planta homogeneizado e coquetel de enzimas
BRPI0910252A2 (pt) 2008-03-07 2015-09-01 Danisco Us Inc Expressão de catalise em trichoderma
KR20100124787A (ko) 2008-03-21 2010-11-29 다니스코 유에스 인크. 바이오매스의 가수분해를 향상시키기 위한 헤미셀룰라아제 강화 조성물
US20110165635A1 (en) 2008-04-21 2011-07-07 Chromatin, Inc. Methods and materials for processing a feedstock
WO2009134878A1 (en) 2008-04-29 2009-11-05 Danisco Us Inc. Swollenin compositions and methods of increasing the efficiency of a cellulase
DK2288698T3 (en) 2008-06-06 2017-03-20 Danisco Us Inc COMPOSITIONS AND PROCEDURES INCLUDING CELLULASE-VARIABLE VARIETIES FOR CELLULOSE-FREE MATERIALS
US8518684B2 (en) 2008-11-18 2013-08-27 Novozymes, Inc. Methods and compositions for degrading cellulosic material
JP5663497B2 (ja) 2009-02-20 2015-02-04 ダニスコ・ユーエス・インク 培養液製剤
BRPI1013167A2 (pt) 2009-02-27 2015-10-06 Iogen Energy Corp enzimas celulases resistentes a lignina
BRPI1011597B1 (pt) 2009-05-29 2019-08-20 Novozymes, Inc. Métodos para degradar um material celulósico, para produzir um produto de fermentação, e para fermentar um material celulósico, e, composição de enzima
WO2010141779A1 (en) 2009-06-03 2010-12-09 Danisco Us Inc. Cellulase variants with improved expression, activity and/or stability, and use thereof
US8772010B2 (en) 2009-06-16 2014-07-08 Codexis, Inc. β-Glucosidase variants
MX353237B (es) 2009-09-23 2018-01-08 Danisco Us Inc Enzimas de glicosil hidrolasa novedosas y usos de las mismas.
JP2013511280A (ja) 2009-11-20 2013-04-04 ダニスコ・ユーエス・インク 改良された特性を有するβ−グルコシダーゼＩ変異体
US10138499B2 (en) * 2009-12-23 2018-11-27 Danisco Us Inc. Methods for improving the efficiency of simultaneous saccharification and fermentation reactions
US8647850B2 (en) * 2009-12-23 2014-02-11 E I Du Pont De Nemours And Company Process for simultaneous saccharification and fermentation for production of ethanol
US8721794B2 (en) 2010-04-28 2014-05-13 E I Du Pont De Nemours And Company Production of high solids syrup from lignocellulosic biomass hydrolysate fermentation broth
US8906235B2 (en) * 2010-04-28 2014-12-09 E I Du Pont De Nemours And Company Process for liquid/solid separation of lignocellulosic biomass hydrolysate fermentation broth
EP2576626B1 (en) 2010-06-01 2018-08-01 California Institute of Technology Stable, functional chimeric cellobiohydrolase class i enzymes
EP2397491A1 (en) 2010-06-21 2011-12-21 Technische Universität Wien LeaA from Trichoderma reesei
EP2611901B1 (en) 2010-08-30 2016-05-11 Novozymes A/S Polypeptides having beta-glucosidase activity, beta-xylosidase activity, or beta-glucosidase and beta-xylosidase activity and polynucleotides encoding same
US8476048B2 (en) 2010-12-17 2013-07-02 E I Du Pont De Nemours And Company Xylose utilizing zymomonas mobilis with improved ethanol production in biomass hydrolysate medium
MX2013010512A (es) 2011-03-17 2013-10-07 Danisco Inc Metodo para reducir la viscosidad en un proceso de sacarificacion.
AU2012228968B2 (en) 2011-03-17 2017-05-18 Danisco Us Inc. Cellulase compositions and methods of using the same for improved conversion of lignocellulosic biomass into fermentable sugars
WO2012125937A2 (en) 2011-03-17 2012-09-20 Danisco Us Inc. Glycosyl hydrolase enzymes and uses thereof for biomass hydrolysis
BR112013027242A2 (pt) 2011-05-10 2016-11-29 Danisco Us Inc anidrases carbônicas termoestáveis e métodos de uso das mesmas
EP2748317B1 (en) 2011-08-22 2017-04-19 Codexis, Inc. Gh61 glycoside hydrolase protein variants and cofactors that enhance gh61 activity
KR20140122704A (ko) 2011-12-13 2014-10-20 다니스코 유에스 인크. 혼합 배양으로부터 제조된 효소 칵테일
CN104781399A (zh) 2012-10-31 2015-07-15 丹尼斯科美国公司 来自稻瘟病菌的β-葡糖苷酶
CA2888757A1 (en) 2012-10-31 2014-05-08 Danisco Us Inc. Compositions and methods of use
MX2015007234A (es) 2012-12-12 2015-10-29 Danisco Us Inc Variantes de celobiohidrolasas.
WO2014188012A1 (es) 2013-05-20 2014-11-27 Abengoa Bioenergía Nuevas Tecnologías, S. A. Expresión de enzimas beta-xilosidasas recombinantes
WO2015017255A1 (en) 2013-07-29 2015-02-05 Danisco Us Inc. Variant enzymes
US20160298157A1 (en) 2013-12-04 2016-10-13 Danisco Us Inc. Compositions comprising a beta-glucosidase polypeptide and methods of use

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