CA2765406C - Stem accelerated isothermal nucleic acid amplification technology - Google Patents

Stem accelerated isothermal nucleic acid amplification technology Download PDF

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Publication number
CA2765406C
CA2765406C CA2765406A CA2765406A CA2765406C CA 2765406 C CA2765406 C CA 2765406C CA 2765406 A CA2765406 A CA 2765406A CA 2765406 A CA2765406 A CA 2765406A CA 2765406 C CA2765406 C CA 2765406C
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Canada
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primer
primers
stem
region
segment
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CA2765406A
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English (en)
French (fr)
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CA2765406A1 (en
Inventor
Laurence Carlo Tisi
Olga Gandelmann
Guy Kiddle
Cathal Joseph Mcelgunn
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Lumora Ltd
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Lumora Ltd
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2765406A 2009-06-15 2010-06-15 Stem accelerated isothermal nucleic acid amplification technology Active CA2765406C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
GB0910302.9 2009-06-15
GBGB0910302.9A GB0910302D0 (en) 2009-06-15 2009-06-15 Nucleic acid amplification
PCT/GB2010/001169 WO2010146349A1 (en) 2009-06-15 2010-06-15 Stem accelerated isothermal nucleic acid amplification technology

Publications (2)

Publication Number Publication Date
CA2765406A1 CA2765406A1 (en) 2010-12-23
CA2765406C true CA2765406C (en) 2019-02-26

Family

ID=40940851

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2765406A Active CA2765406C (en) 2009-06-15 2010-06-15 Stem accelerated isothermal nucleic acid amplification technology

Country Status (10)

Country Link
US (1) US9410190B2 (https=)
EP (1) EP2443253B1 (https=)
JP (2) JP5907864B2 (https=)
CN (1) CN102652176B (https=)
AU (1) AU2010261541B2 (https=)
CA (1) CA2765406C (https=)
ES (1) ES2589079T3 (https=)
GB (1) GB0910302D0 (https=)
WO (1) WO2010146349A1 (https=)
ZA (1) ZA201109493B (https=)

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JP5843614B2 (ja) 2009-01-30 2016-01-13 オックスフォード ナノポア テクノロジーズ リミテッド 膜貫通配列決定における核酸構築物のためのアダプター
AU2011319755B2 (en) 2010-10-27 2017-02-23 President And Fellows Of Harvard College Compositions of toehold primer duplexes and methods of use
ES2537189T3 (es) * 2011-05-24 2015-06-03 Elitech Holding B.V. Detección de estafilococos áureos resistentes a la meticilina
AU2012288629B2 (en) 2011-07-25 2017-02-02 Oxford Nanopore Technologies Limited Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
KR102168813B1 (ko) 2013-03-08 2020-10-22 옥스포드 나노포어 테크놀로지즈 리미티드 효소 정지 방법
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
AU2014233145A1 (en) 2013-03-15 2015-09-17 Theranos Ip Company, Llc Nucleic acid amplification
JP2016521120A (ja) * 2013-03-15 2016-07-21 セラノス， インコーポレイテッド 核酸増幅
US9677981B2 (en) 2013-03-15 2017-06-13 3M Innovative Properties Company Sample concentrator and method of use
US10450595B2 (en) 2013-03-15 2019-10-22 Theranos Ip Company, Llc Nucleic acid amplification
CN105452480B (zh) * 2013-08-09 2020-01-31 基因生物系统股份有限公司 经由剪刀状结构的dna扩增(dasl)
WO2015035260A1 (en) 2013-09-06 2015-03-12 Theranos, Inc. Systems and methods for detecting infectious diseases
CN103497947B (zh) * 2013-09-13 2015-08-12 浙江国际旅行卫生保健中心 一种体外新型快速环介导等温扩增方法、体外检测甲型h1n1流感病毒的试剂及方法
AU2014353462A1 (en) * 2013-11-22 2016-05-05 Theranos Ip Company, Llc Nucleic acid amplification
US11859246B2 (en) 2013-12-11 2024-01-02 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
US11286519B2 (en) 2013-12-11 2022-03-29 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
CN104946737B (zh) 2013-12-11 2019-02-22 安可济控股有限公司 用于检测罕见序列变体的组合物和方法
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US10716587B2 (en) 2014-06-13 2020-07-21 Surgis Medical Llc Surgical device with light
CN106661600B (zh) * 2014-07-16 2021-04-06 唐恩生物科技股份有限公司 用于扩增核酸样品的等温方法
US11559801B2 (en) 2014-11-03 2023-01-24 Tangen Biosciences, Inc. Apparatus and method for cell, spore, or virus capture and disruption
ES2891085T3 (es) 2015-01-30 2022-01-26 Harvard College Obtención de imágenes sin microscopio
CN107208162B (zh) 2015-02-26 2020-12-29 株式会社日立高新技术 核酸分子的构建方法
US10260090B2 (en) 2015-07-16 2019-04-16 Arizona Board Of Regents On Behalf Of Arizona State University Accelerated isothermal amplification of DNA
AU2016334233B2 (en) 2015-10-09 2023-01-05 Accuragen Holdings Limited Methods and compositions for enrichment of amplification products
CN108699505A (zh) 2015-12-03 2018-10-23 安可济控股有限公司 用于形成连接产物的方法和组合物
CN116949140A (zh) 2016-02-17 2023-10-27 哈佛学院院长及董事 分子编程工具
CA3024630A1 (en) 2016-05-16 2017-11-23 Accuragen Holdings Limited Method of improved sequencing by strand identification
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
CN107488656B (zh) * 2016-06-13 2020-07-17 陆欣华 一种核酸等温自扩增方法
CA3033749C (en) 2016-08-15 2026-03-24 Accuragen Holdings Limited Compositions and methods for detecting rare sequence variants
EP3568492B1 (en) * 2017-01-10 2025-01-01 President and Fellows of Harvard College Multiplexed signal amplification
EP3724322A1 (en) 2017-12-14 2020-10-21 Medicinal Genomics Corporation Methods and kits for classifying cannabinoid production in cannabis plants
CN111630179B (zh) 2018-01-26 2024-07-19 哈佛学院院长及董事 邻近检测方法和组合物
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
US12049665B2 (en) 2018-06-12 2024-07-30 Accuragen Holdings Limited Methods and compositions for forming ligation products
US12553080B2 (en) 2021-06-14 2026-02-17 Delta Electronics Int'l (Singapore) Pte Ltd Lamp primer set and method for amplifying nucleic acids using the same
CN114045330B (zh) * 2021-12-23 2024-02-09 川北医学院附属医院 一种基于滑动复制的核酸等温扩增方法
CN118621045A (zh) * 2024-08-14 2024-09-10 天健生物制药（天津）有限公司 基于lamp的鸟分枝杆菌检测的组合物和试剂盒
WO2026050948A1 (zh) * 2024-09-04 2026-03-12 深圳华大生命科学研究院 核酸文库的构建方法及应用

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US5866336A (en) * 1996-07-16 1999-02-02 Oncor, Inc. Nucleic acid amplification oligonucleotides with molecular energy transfer labels and methods based thereon
ATE437225T1 (de) * 2000-09-19 2009-08-15 Eiken Chemical Verfahren zur polynukleotidsynthese
WO2002090538A1 (en) * 2001-05-08 2002-11-14 Eiken Kagaku Kabushiki Kaisha Method of synthesizing nucleic acid
GB0218215D0 (en) 2002-08-06 2002-09-11 Tepnel Medical Ltd Amplification of nucleic acids
ATE532878T1 (de) 2005-08-24 2011-11-15 Life Technologies Corp Verfahren zur quantifizierung von sirnas, mirnas und polymorphen mirnas
JP4580875B2 (ja) * 2006-01-20 2010-11-17 株式会社東芝 核酸検出法のためのプライマーの設計方法
JP2008029333A (ja) 2006-07-03 2008-02-14 Fujifilm Corp 新規遺伝子増幅法に用いられるプライマー
US8252558B2 (en) 2006-10-09 2012-08-28 Oxitec Limited Methods for amplifying and detecting nucleic acid sequences
JP4490988B2 (ja) 2007-04-26 2010-06-30 株式会社東芝 血清アミロイドａ１（ｓａａ１）の遺伝子型を検出するための核酸プライマーセット、キット、及び該プライマーセットを用いた検出方法
US8748100B2 (en) 2007-08-30 2014-06-10 The Chinese University Of Hong Kong Methods and kits for selectively amplifying, detecting or quantifying target DNA with specific end sequences

Also Published As

Publication number Publication date
EP2443253A1 (en) 2012-04-25
ZA201109493B (en) 2015-07-29
JP6106732B2 (ja) 2017-04-05
JP2012529904A (ja) 2012-11-29
CA2765406A1 (en) 2010-12-23
JP5907864B2 (ja) 2016-04-26
AU2010261541B2 (en) 2016-03-10
CN102652176B (zh) 2016-09-28
EP2443253B1 (en) 2016-06-08
US20120157326A1 (en) 2012-06-21
CN102652176A (zh) 2012-08-29
US9410190B2 (en) 2016-08-09
AU2010261541A1 (en) 2012-02-02
WO2010146349A1 (en) 2010-12-23
GB0910302D0 (en) 2009-07-29
ES2589079T3 (es) 2016-11-10
JP2016041070A (ja) 2016-03-31

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