CA2741650C - Fast results hybrid capture assay and system - Google Patents
Fast results hybrid capture assay and system Download PDFInfo
- Publication number
- CA2741650C CA2741650C CA2741650A CA2741650A CA2741650C CA 2741650 C CA2741650 C CA 2741650C CA 2741650 A CA2741650 A CA 2741650A CA 2741650 A CA2741650 A CA 2741650A CA 2741650 C CA2741650 C CA 2741650C
- Authority
- CA
- Canada
- Prior art keywords
- nucleic acid
- antibody
- sample
- double
- hybrid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000003556 assay Methods 0.000 title description 49
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 258
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 251
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 251
- 238000000034 method Methods 0.000 claims abstract description 82
- 239000000523 sample Substances 0.000 claims description 282
- 239000003599 detergent Substances 0.000 claims description 113
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims description 80
- 241000701806 Human papillomavirus Species 0.000 claims description 76
- 238000001514 detection method Methods 0.000 claims description 54
- 239000011324 bead Substances 0.000 claims description 52
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 48
- 239000003153 chemical reaction reagent Substances 0.000 claims description 43
- LBCZOTMMGHGTPH-UHFFFAOYSA-N 2-[2-[4-(2,4,4-trimethylpentan-2-yl)phenoxy]ethoxy]ethanol Chemical compound CC(C)(C)CC(C)(C)C1=CC=C(OCCOCCO)C=C1 LBCZOTMMGHGTPH-UHFFFAOYSA-N 0.000 claims description 35
- 239000007787 solid Substances 0.000 claims description 32
- 239000011534 wash buffer Substances 0.000 claims description 31
- 108091033319 polynucleotide Proteins 0.000 claims description 30
- 102000040430 polynucleotide Human genes 0.000 claims description 30
- 239000002157 polynucleotide Substances 0.000 claims description 30
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 claims description 28
- KXGVEGMKQFWNSR-LLQZFEROSA-N deoxycholic acid Chemical compound C([C@H]1CC2)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 KXGVEGMKQFWNSR-LLQZFEROSA-N 0.000 claims description 28
- 239000012472 biological sample Substances 0.000 claims description 26
- 229960003964 deoxycholic acid Drugs 0.000 claims description 26
- 238000004925 denaturation Methods 0.000 claims description 23
- 230000036425 denaturation Effects 0.000 claims description 23
- 230000005291 magnetic effect Effects 0.000 claims description 21
- 239000000203 mixture Substances 0.000 claims description 21
- 239000003085 diluting agent Substances 0.000 claims description 19
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 239000003550 marker Substances 0.000 claims description 11
- 229920002125 Sokalan® Polymers 0.000 claims description 9
- 239000000758 substrate Substances 0.000 claims description 9
- 230000005298 paramagnetic effect Effects 0.000 claims description 8
- 239000004584 polyacrylic acid Substances 0.000 claims description 8
- 238000005406 washing Methods 0.000 claims description 7
- 239000011780 sodium chloride Substances 0.000 claims description 6
- 239000007983 Tris buffer Substances 0.000 claims description 5
- 230000001268 conjugating effect Effects 0.000 claims description 5
- 230000002068 genetic effect Effects 0.000 claims description 5
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 5
- 229940009976 deoxycholate Drugs 0.000 claims description 2
- GPRLSGONYQIRFK-MNYXATJNSA-N triton Chemical compound [3H+] GPRLSGONYQIRFK-MNYXATJNSA-N 0.000 claims description 2
- 239000007992 BES buffer Substances 0.000 claims 1
- 239000002609 medium Substances 0.000 description 104
- 210000004027 cell Anatomy 0.000 description 34
- 108020004414 DNA Proteins 0.000 description 31
- 238000009396 hybridization Methods 0.000 description 26
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 23
- 239000008188 pellet Substances 0.000 description 20
- 238000012360 testing method Methods 0.000 description 16
- 108091006629 SLC13A2 Proteins 0.000 description 15
- 239000000872 buffer Substances 0.000 description 14
- 238000004458 analytical method Methods 0.000 description 13
- 238000006243 chemical reaction Methods 0.000 description 13
- 239000003391 RNA probe Substances 0.000 description 12
- 239000003755 preservative agent Substances 0.000 description 12
- AJTVSSFTXWNIRG-UHFFFAOYSA-N 2-[bis(2-hydroxyethyl)amino]ethanesulfonic acid Chemical compound OCC[NH+](CCO)CCS([O-])(=O)=O AJTVSSFTXWNIRG-UHFFFAOYSA-N 0.000 description 11
- 108020004518 RNA Probes Proteins 0.000 description 11
- 230000002335 preservative effect Effects 0.000 description 10
- -1 stool) or tissue Substances 0.000 description 10
- 230000008901 benefit Effects 0.000 description 9
- 230000021615 conjugation Effects 0.000 description 9
- 241001465754 Metazoa Species 0.000 description 8
- 238000012545 processing Methods 0.000 description 8
- 108090000623 proteins and genes Proteins 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 230000003321 amplification Effects 0.000 description 7
- 230000003196 chaotropic effect Effects 0.000 description 7
- 238000000605 extraction Methods 0.000 description 7
- 238000011534 incubation Methods 0.000 description 7
- 238000003199 nucleic acid amplification method Methods 0.000 description 7
- 230000003612 virological effect Effects 0.000 description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
- 206010008342 Cervix carcinoma Diseases 0.000 description 6
- 208000006105 Uterine Cervical Neoplasms Diseases 0.000 description 6
- 201000010881 cervical cancer Diseases 0.000 description 6
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 6
- 238000010438 heat treatment Methods 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 6
- 235000018102 proteins Nutrition 0.000 description 6
- 102000004169 proteins and genes Human genes 0.000 description 6
- 230000002829 reductive effect Effects 0.000 description 6
- 238000011282 treatment Methods 0.000 description 6
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 5
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 5
- 206010008263 Cervical dysplasia Diseases 0.000 description 5
- 206010028980 Neoplasm Diseases 0.000 description 5
- 108091028043 Nucleic acid sequence Proteins 0.000 description 5
- 239000003513 alkali Substances 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 239000008280 blood Substances 0.000 description 5
- 230000015556 catabolic process Effects 0.000 description 5
- 208000007951 cervical intraepithelial neoplasia Diseases 0.000 description 5
- 230000000295 complement effect Effects 0.000 description 5
- 238000006731 degradation reaction Methods 0.000 description 5
- 210000002919 epithelial cell Anatomy 0.000 description 5
- 238000006386 neutralization reaction Methods 0.000 description 5
- 239000013612 plasmid Substances 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 241000283707 Capra Species 0.000 description 4
- 241000606153 Chlamydia trachomatis Species 0.000 description 4
- 241000701022 Cytomegalovirus Species 0.000 description 4
- 239000003298 DNA probe Substances 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 201000011510 cancer Diseases 0.000 description 4
- 230000001413 cellular effect Effects 0.000 description 4
- 229940038705 chlamydia trachomatis Drugs 0.000 description 4
- 235000013365 dairy product Nutrition 0.000 description 4
- 239000013024 dilution buffer Substances 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 4
- 210000004408 hybridoma Anatomy 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000007791 liquid phase Substances 0.000 description 4
- 235000013372 meat Nutrition 0.000 description 4
- 238000002156 mixing Methods 0.000 description 4
- 230000003472 neutralizing effect Effects 0.000 description 4
- 239000013610 patient sample Substances 0.000 description 4
- 239000006228 supernatant Substances 0.000 description 4
- 210000001519 tissue Anatomy 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000321096 Adenoides Species 0.000 description 3
- 108091023037 Aptamer Proteins 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- 229920004934 Dacron® Polymers 0.000 description 3
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 3
- 206010035226 Plasma cell myeloma Diseases 0.000 description 3
- 206010036790 Productive cough Diseases 0.000 description 3
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 3
- 210000002534 adenoid Anatomy 0.000 description 3
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 3
- 238000011109 contamination Methods 0.000 description 3
- 230000009260 cross reactivity Effects 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 230000005294 ferromagnetic effect Effects 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000012634 fragment Substances 0.000 description 3
- 238000013038 hand mixing Methods 0.000 description 3
- 230000000670 limiting effect Effects 0.000 description 3
- 210000002751 lymph Anatomy 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 235000013336 milk Nutrition 0.000 description 3
- 239000008267 milk Substances 0.000 description 3
- 210000004080 milk Anatomy 0.000 description 3
- 201000000050 myeloid neoplasm Diseases 0.000 description 3
- 230000007935 neutral effect Effects 0.000 description 3
- 239000002853 nucleic acid probe Substances 0.000 description 3
- 238000009595 pap smear Methods 0.000 description 3
- 210000004910 pleural fluid Anatomy 0.000 description 3
- 239000005020 polyethylene terephthalate Substances 0.000 description 3
- 239000000047 product Substances 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 238000011084 recovery Methods 0.000 description 3
- 210000003296 saliva Anatomy 0.000 description 3
- 150000003839 salts Chemical class 0.000 description 3
- 210000000582 semen Anatomy 0.000 description 3
- 230000035945 sensitivity Effects 0.000 description 3
- 210000003802 sputum Anatomy 0.000 description 3
- 208000024794 sputum Diseases 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 239000002699 waste material Substances 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 241000606161 Chlamydia Species 0.000 description 2
- 241000711573 Coronaviridae Species 0.000 description 2
- WSFSSNUMVMOOMR-UHFFFAOYSA-N Formaldehyde Chemical compound O=C WSFSSNUMVMOOMR-UHFFFAOYSA-N 0.000 description 2
- 206010018612 Gonorrhoea Diseases 0.000 description 2
- 241000341655 Human papillomavirus type 16 Species 0.000 description 2
- XQFRJNBWHJMXHO-RRKCRQDMSA-N IDUR Chemical compound C1[C@H](O)[C@@H](CO)O[C@H]1N1C(=O)NC(=O)C(I)=C1 XQFRJNBWHJMXHO-RRKCRQDMSA-N 0.000 description 2
- 241000588652 Neisseria gonorrhoeae Species 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 244000007853 Sarothamnus scoparius Species 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- 241000224526 Trichomonas Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000012670 alkaline solution Substances 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 239000012620 biological material Substances 0.000 description 2
- 235000019846 buffering salt Nutrition 0.000 description 2
- 239000006227 byproduct Substances 0.000 description 2
- 239000002738 chelating agent Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000005292 diamagnetic effect Effects 0.000 description 2
- 239000004205 dimethyl polysiloxane Substances 0.000 description 2
- VHJLVAABSRFDPM-QWWZWVQMSA-N dithiothreitol Chemical compound SC[C@@H](O)[C@H](O)CS VHJLVAABSRFDPM-QWWZWVQMSA-N 0.000 description 2
- 239000005447 environmental material Substances 0.000 description 2
- 238000011156 evaluation Methods 0.000 description 2
- 239000000834 fixative Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 208000001786 gonorrhea Diseases 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 206010022000 influenza Diseases 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 230000003993 interaction Effects 0.000 description 2
- 235000021056 liquid food Nutrition 0.000 description 2
- 239000012139 lysis buffer Substances 0.000 description 2
- 239000000696 magnetic material Substances 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000013508 migration Methods 0.000 description 2
- 230000005012 migration Effects 0.000 description 2
- 229920001778 nylon Polymers 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 2
- 230000002285 radioactive effect Effects 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 235000021055 solid food Nutrition 0.000 description 2
- 239000006163 transport media Substances 0.000 description 2
- 201000008827 tuberculosis Diseases 0.000 description 2
- 210000002700 urine Anatomy 0.000 description 2
- 210000001215 vagina Anatomy 0.000 description 2
- 239000013598 vector Substances 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 description 1
- QAQSNXHKHKONNS-UHFFFAOYSA-N 1-ethyl-2-hydroxy-4-methyl-6-oxopyridine-3-carboxamide Chemical compound CCN1C(O)=C(C(N)=O)C(C)=CC1=O QAQSNXHKHKONNS-UHFFFAOYSA-N 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 206010003445 Ascites Diseases 0.000 description 1
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 1
- 108020004394 Complementary RNA Proteins 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 229920000858 Cyclodextrin Polymers 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 108020003215 DNA Probes Proteins 0.000 description 1
- BVTJGGGYKAMDBN-UHFFFAOYSA-N Dioxetane Chemical compound C1COO1 BVTJGGGYKAMDBN-UHFFFAOYSA-N 0.000 description 1
- 108010067770 Endopeptidase K Proteins 0.000 description 1
- 241000711475 Feline infectious peritonitis virus Species 0.000 description 1
- 208000034826 Genetic Predisposition to Disease Diseases 0.000 description 1
- 108020004996 Heterogeneous Nuclear RNA Proteins 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101000739160 Homo sapiens Secretoglobin family 3A member 1 Proteins 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- 102000008394 Immunoglobulin Fragments Human genes 0.000 description 1
- 108010021625 Immunoglobulin Fragments Proteins 0.000 description 1
- 108060004795 Methyltransferase Proteins 0.000 description 1
- 241000699666 Mus <mouse, genus> Species 0.000 description 1
- 241000699670 Mus sp. Species 0.000 description 1
- 108020003217 Nuclear RNA Proteins 0.000 description 1
- 102000043141 Nuclear RNA Human genes 0.000 description 1
- 101710163270 Nuclease Proteins 0.000 description 1
- 108020002230 Pancreatic Ribonuclease Proteins 0.000 description 1
- 102000005891 Pancreatic ribonuclease Human genes 0.000 description 1
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 229920001213 Polysorbate 20 Polymers 0.000 description 1
- 239000004793 Polystyrene Substances 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 102100037268 Secretoglobin family 3A member 1 Human genes 0.000 description 1
- 108020004682 Single-Stranded DNA Proteins 0.000 description 1
- 101710120037 Toxin CcdB Proteins 0.000 description 1
- 108020004566 Transfer RNA Proteins 0.000 description 1
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 description 1
- 229920004890 Triton X-100 Polymers 0.000 description 1
- 239000013504 Triton X-100 Substances 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 1
- 108020000999 Viral RNA Proteins 0.000 description 1
- 238000001261 affinity purification Methods 0.000 description 1
- 125000005211 alkyl trimethyl ammonium group Chemical group 0.000 description 1
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960001716 benzalkonium Drugs 0.000 description 1
- 239000003150 biochemical marker Substances 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 239000005018 casein Substances 0.000 description 1
- BECPQYXYKAMYBN-UHFFFAOYSA-N casein, tech. Chemical compound NCCCCC(C(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(CC(C)C)N=C(O)C(CCC(O)=O)N=C(O)C(CC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(C(C)O)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=N)N=C(O)C(CCC(O)=O)N=C(O)C(CCC(O)=O)N=C(O)C(COP(O)(O)=O)N=C(O)C(CCC(O)=N)N=C(O)C(N)CC1=CC=CC=C1 BECPQYXYKAMYBN-UHFFFAOYSA-N 0.000 description 1
- 235000021240 caseins Nutrition 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000012512 characterization method Methods 0.000 description 1
- 150000003841 chloride salts Chemical class 0.000 description 1
- 101150005988 cin2 gene Proteins 0.000 description 1
- 238000004737 colorimetric analysis Methods 0.000 description 1
- 239000002299 complementary DNA Substances 0.000 description 1
- 239000003184 complementary RNA Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 230000002380 cytological effect Effects 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 239000003398 denaturant Substances 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000002889 diamagnetic material Substances 0.000 description 1
- WQABCVAJNWAXTE-UHFFFAOYSA-N dimercaprol Chemical compound OCC(S)CS WQABCVAJNWAXTE-UHFFFAOYSA-N 0.000 description 1
- 230000005611 electricity Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000003623 enhancer Substances 0.000 description 1
- 210000000981 epithelium Anatomy 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000003302 ferromagnetic material Substances 0.000 description 1
- 239000011888 foil Substances 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 229960000789 guanidine hydrochloride Drugs 0.000 description 1
- ZRALSGWEFCBTJO-UHFFFAOYSA-O guanidinium Chemical compound NC(N)=[NH2+] ZRALSGWEFCBTJO-UHFFFAOYSA-O 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- DCPMPXBYPZGNDC-UHFFFAOYSA-N hydron;methanediimine;chloride Chemical compound Cl.N=C=N DCPMPXBYPZGNDC-UHFFFAOYSA-N 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 238000003018 immunoassay Methods 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 238000013101 initial test Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000006101 laboratory sample Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000006166 lysate Substances 0.000 description 1
- ADKOXSOCTOWDOP-UHFFFAOYSA-L magnesium;aluminum;dihydroxide;trihydrate Chemical compound O.O.O.[OH-].[OH-].[Mg+2].[Al] ADKOXSOCTOWDOP-UHFFFAOYSA-L 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 108020004999 messenger RNA Proteins 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 239000002923 metal particle Substances 0.000 description 1
- 238000002493 microarray Methods 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- ODLHGICHYURWBS-FOSILIAISA-N molport-023-220-444 Chemical compound CC(O)COC[C@@H]([C@@H]([C@H]([C@@H]1O)O)O[C@@H]2O[C@H]([C@H](O[C@@H]3O[C@@H](COCC(C)O)[C@@H]([C@H]([C@@H]3O)O)O[C@@H]3O[C@@H](COCC(C)O)[C@@H]([C@H]([C@@H]3O)O)O[C@@H]3O[C@@H](COCC(C)O)[C@@H]([C@H]([C@@H]3O)O)O[C@@H]3O[C@@H](COCC(C)O)[C@@H]([C@H]([C@@H]3O)O)O3)[C@@H](O)[C@@H]2O)COCC(O)C)O[C@H]1O[C@@H]1[C@@H](O)[C@H](O)[C@H]3O[C@H]1COCC(C)O ODLHGICHYURWBS-FOSILIAISA-N 0.000 description 1
- 230000035772 mutation Effects 0.000 description 1
- 125000001400 nonyl group Chemical group [H]C([*])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])[H] 0.000 description 1
- 210000000633 nuclear envelope Anatomy 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 229920002113 octoxynol Polymers 0.000 description 1
- 239000002907 paramagnetic material Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000002205 phenol-chloroform extraction Methods 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 description 1
- 229920002223 polystyrene Polymers 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000002203 pretreatment Methods 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 230000000135 prohibitive effect Effects 0.000 description 1
- 230000004850 protein–protein interaction Effects 0.000 description 1
- 239000000941 radioactive substance Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 108020004418 ribosomal RNA Proteins 0.000 description 1
- 229940016590 sarkosyl Drugs 0.000 description 1
- 108700004121 sarkosyl Proteins 0.000 description 1
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 1
- 238000013207 serial dilution Methods 0.000 description 1
- 244000000033 sexually transmitted pathogen Species 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- KSAVQLQVUXSOCR-UHFFFAOYSA-M sodium lauroyl sarcosinate Chemical compound [Na+].CCCCCCCCCCCC(=O)N(C)CC([O-])=O KSAVQLQVUXSOCR-UHFFFAOYSA-M 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000004989 spleen cell Anatomy 0.000 description 1
- 210000004988 splenocyte Anatomy 0.000 description 1
- 238000012430 stability testing Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 125000002088 tosyl group Chemical group [H]C1=C([H])C(=C([H])C([H])=C1C([H])([H])[H])S(*)(=O)=O 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
- 238000011269 treatment regimen Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6804—Nucleic acid analysis using immunogens
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/70—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
- C12Q1/701—Specific hybridization probes
- C12Q1/708—Specific hybridization probes for papilloma
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (5)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10868708P | 2008-10-27 | 2008-10-27 | |
| US61/108,687 | 2008-10-27 | ||
| US17484809P | 2009-05-01 | 2009-05-01 | |
| US61/174,848 | 2009-05-01 | ||
| PCT/US2009/062061 WO2010062556A1 (en) | 2008-10-27 | 2009-10-26 | Fast results hybrid capture assay and system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2741650A1 CA2741650A1 (en) | 2010-06-03 |
| CA2741650C true CA2741650C (en) | 2017-09-05 |
Family
ID=41401545
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2741650A Expired - Fee Related CA2741650C (en) | 2008-10-27 | 2009-10-26 | Fast results hybrid capture assay and system |
Country Status (9)
| Country | Link |
|---|---|
| US (3) | US8288520B2 (https=) |
| EP (1) | EP2347011B1 (https=) |
| JP (2) | JP2012506705A (https=) |
| CN (1) | CN102203293A (https=) |
| AU (1) | AU2009320213B2 (https=) |
| CA (1) | CA2741650C (https=) |
| ES (1) | ES2622062T3 (https=) |
| TW (3) | TWI394839B (https=) |
| WO (2) | WO2010062546A1 (https=) |
Families Citing this family (39)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8703492B2 (en) | 2007-04-06 | 2014-04-22 | Qiagen Gaithersburg, Inc. | Open platform hybrid manual-automated sample processing system |
| US8357538B2 (en) * | 2007-04-06 | 2013-01-22 | Qiagen Gaithersburg, Inc. | Automated assay and system |
| WO2010062546A1 (en) * | 2008-10-27 | 2010-06-03 | Qiagen Gaithersburg Inc. | Fast results hybrid capture assay on an automated platform |
| WO2010078574A1 (en) | 2009-01-05 | 2010-07-08 | Afl Telecommunications Llc | Universal mounting bracket |
| JP5826752B2 (ja) | 2009-09-14 | 2015-12-02 | キアジェン ゲイサーズバーグ インコーポレイテッド | 細胞学用培地に固定された組織サンプルから核酸またはタンパク質を回収するための組成物および方法 |
| US9953141B2 (en) | 2009-11-18 | 2018-04-24 | Becton, Dickinson And Company | Laboratory central control unit method and system |
| US9107652B2 (en) * | 2009-11-19 | 2015-08-18 | Qiagen Gaithersburg, Inc. | Sampling devices and methods |
| CA2787781A1 (en) | 2010-01-29 | 2011-08-04 | Qiagen Gaithersburg, Inc. | Method of determining and confirming the presence of an hpv in a sample |
| CA2787924A1 (en) | 2010-01-29 | 2011-08-04 | Qiagen Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
| JP2013528049A (ja) | 2010-05-19 | 2013-07-08 | キアゲン ガイサーズバーグ アイエヌシー． | 核酸の配列特異的な精製及び多重分析のための方法及び組成物 |
| JP6153866B2 (ja) * | 2010-05-25 | 2017-06-28 | キアゲン ガイサーズバーグ アイエヌシー． | 迅速なハイブリッド捕捉アッセイ、及び関連する戦略的に切断されたプローブ |
| CN103597095B (zh) | 2011-02-24 | 2016-08-17 | 奇亚根盖瑟斯堡股份有限公司 | 用于检测hpv核酸的材料和方法 |
| ES2625288T3 (es) | 2011-04-15 | 2017-07-19 | The Johns Hopkins University | Sistema de secuenciación segura |
| US9604213B2 (en) | 2012-02-13 | 2017-03-28 | Neumodx Molecular, Inc. | System and method for processing and detecting nucleic acids |
| US9637775B2 (en) | 2012-02-13 | 2017-05-02 | Neumodx Molecular, Inc. | System and method for processing biological samples |
| US11485968B2 (en) | 2012-02-13 | 2022-11-01 | Neumodx Molecular, Inc. | Microfluidic cartridge for processing and detecting nucleic acids |
| US11648561B2 (en) | 2012-02-13 | 2023-05-16 | Neumodx Molecular, Inc. | System and method for processing and detecting nucleic acids |
| WO2013123035A1 (en) | 2012-02-13 | 2013-08-22 | Molecular Systems Corporation | System and method for processing and detecting nucleic acids |
| WO2013123440A1 (en) * | 2012-02-17 | 2013-08-22 | Institute For Systems Biology | Rnase h-based rna profiling |
| US9260763B2 (en) | 2012-10-22 | 2016-02-16 | Qiagen Gaithersburg, Inc. | Sample processing method using tube strips and tube strip holder |
| US9310281B2 (en) | 2012-10-22 | 2016-04-12 | Qiagen Gaithersburg, Inc. | Automated pelletized sample blotting apparatus and methods |
| US9435718B2 (en) | 2012-10-22 | 2016-09-06 | Qiagen Gaithersburg, Inc. | Automated pelletized sample decanting apparatus and methods |
| US20140120544A1 (en) | 2012-10-25 | 2014-05-01 | Neumodx Molecular, Inc. | Method and materials for isolation of nucleic acid materials |
| AU2013338393C1 (en) | 2012-10-29 | 2024-07-25 | The Johns Hopkins University | Papanicolaou test for ovarian and endometrial cancers |
| JP6727127B2 (ja) | 2013-12-20 | 2020-07-29 | バイオメッド バレー ディスカバリーズ，インコーポレイティド | Ｐｉ３ｋ／ａｋｔシグナル経路阻害剤およびｅｒｋ阻害剤の組み合わせを使用するがん処置 |
| WO2015095842A2 (en) | 2013-12-20 | 2015-06-25 | Biomed Valley Discoveries, Inc. | Methods and compositions for treating non-erk mapk pathway inhibitor-resistant cancers |
| JP6678583B2 (ja) | 2013-12-20 | 2020-04-22 | バイオメッド バレー ディスカバリーズ，インコーポレイティド | ２型ｍｅｋ阻害剤およびｅｒｋ阻害剤の組み合わせを用いるがん処置 |
| CA3240745A1 (en) | 2013-12-20 | 2015-06-25 | Biomed Valley Discoveries, Inc. | Cancer treatment using combinations of erk and raf inhibitors |
| AU2014368927B2 (en) | 2013-12-20 | 2018-10-25 | Biomed Valley Discoveries, Inc. | Cancer treatments using combinations of CDK and ERK inhibitors |
| CN104020291B (zh) * | 2014-06-26 | 2016-03-30 | 南京大学 | 一种基于酶联免疫分析的hpv核酸检测试剂盒及其应用 |
| WO2016011798A1 (zh) * | 2015-01-27 | 2016-01-28 | 深圳华大基因研究院 | 用于保存生物样品的稳定剂 |
| EP3314269A4 (en) | 2015-06-26 | 2019-01-23 | Abbott Laboratories | REACTION TANK EXCHANGER DEVICE FOR DIAGNOSTIC ANALYZER |
| US11286531B2 (en) | 2015-08-11 | 2022-03-29 | The Johns Hopkins University | Assaying ovarian cyst fluid |
| WO2018160907A1 (en) * | 2017-03-03 | 2018-09-07 | Counsyl, Inc. | Extraction of nucleic acids for reduced probe count variability |
| IL319255A (en) | 2017-08-07 | 2025-04-01 | Univ Johns Hopkins | Methods and materials for cancer assessment and treatment |
| CA3142023A1 (en) * | 2019-05-28 | 2020-12-03 | Case Western Reserve University | Compositions and methods for preserving dna methylation |
| JP2023513606A (ja) | 2020-02-14 | 2023-03-31 | ザ・ジョンズ・ホプキンス・ユニバーシティー | 核酸を評価するための方法および材料 |
| AU2021281223A1 (en) | 2020-05-27 | 2023-01-05 | Case Western Reserve University | Compositions and methods for preserving DNA methylation |
| US11421217B1 (en) * | 2021-04-07 | 2022-08-23 | Convergent Genomics, Inc. | Methods and compositions for collection and preservation of biologicals |
Family Cites Families (196)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4732847A (en) * | 1981-06-09 | 1988-03-22 | University Of Hawaii | Monoclonal antibodies for DNA-RNA hybrid complexes and their uses |
| FI63596C (fi) | 1981-10-16 | 1983-07-11 | Orion Yhtymae Oy | Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser |
| US4486536A (en) * | 1982-05-28 | 1984-12-04 | Smithkline Diagnostics, Inc. | Specimen slide for occult blood testing |
| US4865980A (en) * | 1982-12-29 | 1989-09-12 | University Of Hawaii | Monoclonal antibodies for DNA-RNA hybrid complexes and their uses |
| US5288611A (en) * | 1983-01-10 | 1994-02-22 | Gen-Probe Incorporated | Method for detecting, identifying, and quantitating organisms and viruses |
| EP0131052B1 (en) | 1983-01-10 | 1993-12-08 | Gen-Probe Incorporated | Method for detecting, identifying, and quantitating organisms and viruses |
| US5200313A (en) * | 1983-08-05 | 1993-04-06 | Miles Inc. | Nucleic acid hybridization assay employing detectable anti-hybrid antibodies |
| EP0144914A3 (en) | 1983-12-12 | 1986-08-13 | Miles Inc. | Hybridization assay employing labeled pairs of hybrid binding reagents |
| US4743535A (en) | 1984-11-07 | 1988-05-10 | Miles Inc. | Hybridization assay employing labeled probe and anti-hybrid |
| US7138505B1 (en) * | 1984-01-12 | 2006-11-21 | Novartis Vaccines And Diagnostics, Inc. | Factor VIII:C nucleic acid molecules |
| AU3844485A (en) * | 1984-02-09 | 1985-08-15 | Enzo Biochem Inc. | Heterologous detection of cabeled dna |
| FI71768C (fi) * | 1984-02-17 | 1987-02-09 | Orion Yhtymae Oy | Foerbaettrade nykleinsyrareagenser och foerfarande foer deras framstaellning. |
| US4512442A (en) * | 1984-03-30 | 1985-04-23 | Westinghouse Electric Corp. | Method and apparatus for improving the servicing of an elevator system |
| US6221581B1 (en) * | 1984-04-27 | 2001-04-24 | Enzo Diagnostics, Inc. | Processes for detecting polynucleotides, determining genetic mutations or defects in genetic material, separating or isolating nucleic acid of interest from samples, and useful compositions of matter and multihybrid complex compositions |
| CA1260372A (en) * | 1984-04-27 | 1989-09-26 | Elazar Rabbani | Hybridization method for the detection of genetic materials |
| ZA853756B (en) * | 1984-06-01 | 1986-01-29 | Miles Lab | Nucleic acid hybridization assay employing immobilized rna probes |
| CA1253777A (en) | 1984-06-01 | 1989-05-09 | Robert J. Carrico | Nucleic acid hybridization assay employing immobilized rna probes |
| IL75648A0 (en) | 1984-07-05 | 1985-10-31 | Gen Probe Inc | Accelerated nucleic acid reassociation method |
| US4563417A (en) | 1984-08-31 | 1986-01-07 | Miles Laboratories, Inc. | Nucleic acid hybridization assay employing antibodies to intercalation complexes |
| US4775619A (en) | 1984-10-16 | 1988-10-04 | Chiron Corporation | Polynucleotide determination with selectable cleavage sites |
| US4689294A (en) | 1984-11-19 | 1987-08-25 | Miles Laboratories, Inc. | Enhancement of hybridization of nucleic acids by anionic polymers |
| US4751177A (en) * | 1985-06-13 | 1988-06-14 | Amgen | Methods and kits for performing nucleic acid hybridization assays |
| US5641630A (en) | 1985-06-13 | 1997-06-24 | Amgen Inc. | Method and kit for performing nucleic acid hybridization assays |
| IL79112A (en) | 1985-06-13 | 1992-01-15 | Abbott Lab | Method for the isolation of a nucleic acid sequence and kit for performing a hybridization assay |
| US4710472A (en) * | 1985-09-25 | 1987-12-01 | The United States Of America As Represented By The Secretary Of The Navy | Magnetic separation device |
| US4868105A (en) * | 1985-12-11 | 1989-09-19 | Chiron Corporation | Solution phase nucleic acid sandwich assay |
| US5750338A (en) * | 1986-10-23 | 1998-05-12 | Amoco Corporation | Target and background capture methods with amplification for affinity assays |
| US5220013A (en) * | 1986-11-17 | 1993-06-15 | Scios Nova Inc. | DNA sequence useful for the detection of Alzheimer's disease |
| JP3116353B2 (ja) | 1986-11-24 | 2000-12-11 | ジエン‐プローブ・インコーポレイテツド | 非ウイルス微生物の検出及び／又は定量用核酸プローブ |
| AU601021B2 (en) | 1987-03-11 | 1990-08-30 | Molecular Diagnostics, Inc. | Assay for necleic acid sequences in a sample |
| IL85551A0 (en) | 1987-04-01 | 1988-08-31 | Miles Inc | Rapid hybridization assay and reagent system used therein |
| US5359100A (en) | 1987-10-15 | 1994-10-25 | Chiron Corporation | Bifunctional blocked phosphoramidites useful in making nucleic acid mutimers |
| US5656731A (en) * | 1987-10-15 | 1997-08-12 | Chiron Corporation | Nucleic acid-amplified immunoassay probes |
| ATE133454T1 (de) * | 1987-12-01 | 1996-02-15 | Amoco Corp | Nachweis von salmonella |
| US5082830A (en) | 1988-02-26 | 1992-01-21 | Enzo Biochem, Inc. | End labeled nucleotide probe |
| EP0333465B1 (en) | 1988-03-18 | 1994-07-13 | Baylor College Of Medicine | Mutation detection by competitive oligonucleotide priming |
| US6326136B1 (en) | 1988-04-01 | 2001-12-04 | Digene Corporation | Macromolecular conjugate made using unsaturated aldehydes |
| US5374524A (en) * | 1988-05-10 | 1994-12-20 | E. I. Du Pont De Nemours And Company | Solution sandwich hybridization, capture and detection of amplified nucleic acids |
| NO165894C (no) | 1988-05-24 | 1991-04-24 | Gunnar Paulsen | Analysemetode for gener. |
| EP0703296B1 (en) | 1988-09-29 | 1998-07-22 | Chiron Corporation | Polynucleotide determination by displacement of a strand on a capture probe |
| GB8823869D0 (en) * | 1988-10-12 | 1988-11-16 | Medical Res Council | Production of antibodies |
| JP3046837B2 (ja) * | 1989-03-10 | 2000-05-29 | バイシス・インコーポレーテツド | 固定化されたオリゴヌクレオチドのプローブおよびそれらの使用 |
| US5106727A (en) * | 1989-04-27 | 1992-04-21 | Life Technologies, Inc. | Amplification of nucleic acid sequences using oligonucleotides of random sequences as primers |
| EP0405913B1 (en) | 1989-06-30 | 1998-12-23 | Chiron Corporation | Hydrophobic nucleic acid probe |
| US5116734A (en) * | 1989-09-01 | 1992-05-26 | Digene Diagnostics, Inc. | Highly sensitive method for detecting peroxidase |
| JPH05501650A (ja) | 1989-12-01 | 1993-04-02 | バイシス・インコーポレーテツド | Ｈｐｖ転写物の検出 |
| US5629153A (en) * | 1990-01-10 | 1997-05-13 | Chiron Corporation | Use of DNA-dependent RNA polymerase transcripts as reporter molecules for signal amplification in nucleic acid hybridization assays |
| US5792606A (en) | 1990-02-26 | 1998-08-11 | Boehringer Mannheim Gmbh | Nucleic acid hybridization based assay for determining a substance of interest |
| CA2039517C (en) * | 1990-04-03 | 2006-11-07 | David Segev | Dna probe signal amplification |
| US5695926A (en) | 1990-06-11 | 1997-12-09 | Bio Merieux | Sandwich hybridization assays using very short capture probes noncovalently bound to a hydrophobic support |
| US5545806A (en) * | 1990-08-29 | 1996-08-13 | Genpharm International, Inc. | Ransgenic non-human animals for producing heterologous antibodies |
| US5484699A (en) * | 1990-09-28 | 1996-01-16 | Abbott Laboratories | Nucleotide sequences useful as type specific probes, PCR primers and LCR probes for the amplification and detection of human papilloma virus, and related kits and methods |
| US5210704A (en) * | 1990-10-02 | 1993-05-11 | Technology International Incorporated | System for prognosis and diagnostics of failure and wearout monitoring and for prediction of life expectancy of helicopter gearboxes and other rotating equipment |
| US5474895A (en) | 1990-11-14 | 1995-12-12 | Siska Diagnostics Inc. | Non-isotopic detection of nucleic acids using a polystyrene support-based sandwich hybridization assay and compositions useful therefor |
| DE69233158T2 (de) * | 1991-03-07 | 2004-05-13 | Connaught Technology Corp., Greenville | Gentechnologisch hergestellter stamm für impfstoffe |
| US5283175A (en) * | 1991-04-15 | 1994-02-01 | The Research Foundation Of State University Of New York | Genus-specific oligomers of Borrelia and methods of using same |
| US5556748A (en) | 1991-07-30 | 1996-09-17 | Xenopore Corporation | Methods of sandwich hybridization for the quantitative analysis of oligonucleotides |
| US5981179A (en) * | 1991-11-14 | 1999-11-09 | Digene Diagnostics, Inc. | Continuous amplification reaction |
| ES2145750T3 (es) | 1991-11-14 | 2000-07-16 | Dgi Inc | Kit y dosificado que utiliza la hibridacion no radioactiva. |
| US5747244A (en) | 1991-12-23 | 1998-05-05 | Chiron Corporation | Nucleic acid probes immobilized on polystyrene surfaces |
| EP0618924B1 (en) | 1991-12-23 | 2001-02-14 | Bayer Corporation | Hbv amplifier probes for use in solution phase sandwich hybridization assays |
| US5424413A (en) * | 1992-01-22 | 1995-06-13 | Gen-Probe Incorporated | Branched nucleic acid probes |
| WO1994006859A1 (en) | 1992-09-15 | 1994-03-31 | The Dow Chemical Company | Impact modification of thermoplastics |
| DE69327454T2 (de) * | 1992-10-09 | 2000-05-11 | Vysis, Inc. | Analytisches verfahren |
| US5400267A (en) * | 1992-12-08 | 1995-03-21 | Hemostatix Corporation | Local in-device memory feature for electrically powered medical equipment |
| EP0688366B1 (en) | 1993-01-15 | 2002-05-22 | The Public Health Research Institute Of The City Of New York, Inc. | Sensitive nucleic acid sandwich hybridization assays and kits |
| WO1994028156A1 (en) * | 1993-05-20 | 1994-12-08 | Dana-Farber Cancer Institute | Compositions and methods for treatment of herpesvirus infections |
| WO1995003430A1 (en) | 1993-07-23 | 1995-02-02 | Gen-Probe Incorporated | Methods for enhancing nucleic acid amplification |
| DE4331012A1 (de) * | 1993-09-13 | 1995-03-16 | Bayer Ag | Nukleinsäuren-bindende Oligomere mit N-Verzweigung für Therapie und Diagnostik |
| US5681697A (en) | 1993-12-08 | 1997-10-28 | Chiron Corporation | Solution phase nucleic acid sandwich assays having reduced background noise and kits therefor |
| DK145493D0 (da) | 1993-12-23 | 1993-12-23 | Dako As | Antistof |
| AUPM358894A0 (en) * | 1994-01-31 | 1994-02-24 | Csl Limited | Modified papilloma virus l2 protein and vlps formed therefrom |
| DE69535500D1 (de) * | 1994-02-14 | 2007-07-05 | Macfarlane Burnet Ct For Medic | Nicht pathogene stämme des hiv-1 |
| ATE174066T1 (de) * | 1994-04-04 | 1998-12-15 | Ciba Corning Diagnostics Corp | Hybridisation-ligitation-verfahren zum nachweis von spezifischen dns sequenzen |
| CA2126952C (en) * | 1994-06-28 | 2010-12-14 | Sithian Pandian | Probe, kit, and method of amplification for increasing the sensitivity of nucleic acid hybridization assays |
| US5681702A (en) * | 1994-08-30 | 1997-10-28 | Chiron Corporation | Reduction of nonspecific hybridization by using novel base-pairing schemes |
| JP3093116B2 (ja) * | 1994-09-30 | 2000-10-03 | 株式会社豊田中央研究所 | 核酸検出方法 |
| US6057099A (en) | 1994-12-02 | 2000-05-02 | Intelligene Ltd. | Detection of nucleic acid sequences |
| US5747248A (en) * | 1994-12-05 | 1998-05-05 | Chiron Corporation | Discontinuous probe design using hybritope mapping |
| US20030104361A1 (en) | 1997-09-29 | 2003-06-05 | Susan Weininger | Method of detection of nucleic acids with a specific sequence composition |
| CA2139070C (en) * | 1994-12-23 | 2010-03-30 | Burton W. Blais | Method for enhancing detection ability of nucleic acid assays employing polymerase chain reaction |
| US5731153A (en) * | 1996-08-26 | 1998-03-24 | The Regents Of The University Of California | Identification of random nucleic acid sequence aberrations using dual capture probes which hybridize to different chromosome regions |
| US5888724A (en) * | 1995-02-17 | 1999-03-30 | The Trustees Of Columbia University In The City Of New York | Detection of high oncogenic-risk papilloma virus in high grade cervical lesions and cancers by a PCR/ELISA assay |
| US6083925A (en) * | 1995-06-07 | 2000-07-04 | Connaught Laboratories Limited | Nucleic acid respiratory syncytial virus vaccines |
| EP0832280A2 (en) | 1995-06-07 | 1998-04-01 | Abbott Laboratories | Probe masking method of reducing background in an amplification reaction |
| FR2737502B1 (fr) * | 1995-07-31 | 1997-10-24 | Genset Sa | Procede de detection d'acides nucleiques utilisant des sondes nucleotidiques permettant a la fois une capture specifique et une detection |
| CA2237891C (en) * | 1995-11-15 | 2013-07-30 | Gen-Probe Incorporated | Nucleic acid probes complementary to human papillomavirus nucleic acid and related methods and kits |
| EP2332958B1 (en) | 1996-02-09 | 2016-04-20 | Cornell Research Foundation, Inc. | Detection of nucleic and sequence differences using the ligase detection reaction with addressable arrays |
| US6453190B1 (en) * | 1996-02-15 | 2002-09-17 | Biosense, Inc. | Medical probes with field transducers |
| US20010039007A1 (en) * | 1996-10-15 | 2001-11-08 | Robert Hallowitz | Methods and compositions for determining latent viral load |
| US5853993A (en) | 1996-10-21 | 1998-12-29 | Hewlett-Packard Company | Signal enhancement method and kit |
| US6117631A (en) | 1996-10-29 | 2000-09-12 | Polyprobe, Inc. | Detection of antigens via oligonucleotide antibody conjugates |
| GB9624165D0 (en) | 1996-11-19 | 1997-01-08 | Amdex A S | Use of nucleic acids bound to carrier macromolecules |
| US6110676A (en) * | 1996-12-04 | 2000-08-29 | Boston Probes, Inc. | Methods for suppressing the binding of detectable probes to non-target sequences in hybridization assays |
| US6331181B1 (en) * | 1998-12-08 | 2001-12-18 | Intuitive Surgical, Inc. | Surgical robotic tools, data architecture, and use |
| US6132368A (en) * | 1996-12-12 | 2000-10-17 | Intuitive Surgical, Inc. | Multi-component telepresence system and method |
| US20020034737A1 (en) | 1997-03-04 | 2002-03-21 | Hyseq, Inc. | Methods and compositions for detection or quantification of nucleic acid species |
| JPH10210982A (ja) * | 1997-01-31 | 1998-08-11 | Fuji Yakuhin Kogyo Kk | 新規なタンパク質 |
| US6151039A (en) * | 1997-06-04 | 2000-11-21 | Hewlett-Packard Company | Ink level estimation using drop count and ink level sense |
| WO1998059044A1 (en) * | 1997-06-25 | 1998-12-30 | Tularik Inc. | High-throughput screening assays for modulators of primase activity |
| US6043038A (en) | 1998-03-31 | 2000-03-28 | Tularik, Inc. | High-throughput screening assays for modulators of primase activity |
| US5843995A (en) | 1997-07-07 | 1998-12-01 | University Of Medicine And Dentistry Of New Jersey | Inhibition of HIV-1 replication using oligocarbamate derivatives |
| IL121348A0 (en) * | 1997-07-21 | 1998-04-05 | Bio Rad Lab Israel Inc | System and method for device monitoring |
| AU741141B2 (en) | 1997-11-04 | 2001-11-22 | Roche Diagnostics Gmbh | Specific and sensitive method for detecting nucleic acids |
| US6225053B1 (en) | 1997-12-12 | 2001-05-01 | Digene Corporation | Detection of hepatitis B virus |
| WO1999031273A2 (en) | 1997-12-12 | 1999-06-24 | Digene Corporation | Universal collection medium |
| US20030096232A1 (en) | 1997-12-19 | 2003-05-22 | Kris Richard M. | High throughput assay system |
| ATE506449T1 (de) | 1997-12-22 | 2011-05-15 | Hitachi Chemical Co Ltd | Direkte rt-pcr auf oligonukleotid-immobilisierten pcr-mikroplatten |
| EP1047794A2 (en) | 1998-01-13 | 2000-11-02 | BioChip Technologies GmbH | Method for the detection or nucleic acid of nucleic acid sequences |
| EP1051523A2 (en) | 1998-02-02 | 2000-11-15 | Amersham Pharmacia Biotech AB | Nucleic acid analysis method |
| US7399589B2 (en) | 1998-02-06 | 2008-07-15 | Digene Corporation | Immunological detection of RNA:DNA hybrids on microarrays |
| US6686151B1 (en) | 1998-02-06 | 2004-02-03 | Digene Corporation | Immunological detection of RNA:DNA hybrids on microarrays |
| US5994079A (en) | 1998-02-06 | 1999-11-30 | Digene Corporation | Direct detection of RNA mediated by reverse transcriptase lacking RNAse H function |
| DE69836907T2 (de) * | 1998-02-10 | 2007-11-08 | Biosense Webster, Inc., Diamond Bar | Sondenanordnung zur verbesserten katheterkalibrierung |
| ES2212251T3 (es) | 1998-03-23 | 2004-07-16 | Spal S.R.L. | Ventilador de flujo axial. |
| US6183956B1 (en) | 1998-03-31 | 2001-02-06 | Tularik, Incorporated | High throughput in vitro screening assays for transcription modulators |
| US6246200B1 (en) * | 1998-08-04 | 2001-06-12 | Intuitive Surgical, Inc. | Manipulator positioning linkage for robotic surgery |
| US20020004198A1 (en) * | 1998-12-16 | 2002-01-10 | Hardwicke Mary Ann | Novel anti-infectives |
| US20010055766A1 (en) | 1999-04-02 | 2001-12-27 | Alexander Aristarkhov | Immunosorbant assay using branched bis-biotin/avidin/multiple label complex as a detection reagent |
| AU4051300A (en) | 1999-04-02 | 2000-10-23 | Tropix, Inc. | High throughput and high sensitivity detection assays |
| US7019822B1 (en) | 1999-04-29 | 2006-03-28 | Mss, Inc. | Multi-grade object sorting system and method |
| US6544732B1 (en) * | 1999-05-20 | 2003-04-08 | Illumina, Inc. | Encoding and decoding of array sensors utilizing nanocrystals |
| US6200746B1 (en) | 1999-08-25 | 2001-03-13 | Pharmacia & Upjohn Company | Methods of identifying anti-viral agents |
| US6611793B1 (en) * | 1999-09-07 | 2003-08-26 | Scimed Life Systems, Inc. | Systems and methods to identify and disable re-use single use devices based on detecting environmental changes |
| US20060275784A1 (en) | 1999-10-26 | 2006-12-07 | Ventana Medical Systems, Inc. | Detection of Human Papilloma Virus in Papanicolaou (Pap) Smears |
| US6893819B1 (en) | 2000-11-21 | 2005-05-17 | Stratagene California | Methods for detection of a nucleic acid by sequential amplification |
| US6436662B1 (en) | 2000-04-04 | 2002-08-20 | Digene Corporation | Device and method for cytology slide preparation |
| US6521190B1 (en) | 2000-05-19 | 2003-02-18 | Digene Corporation | Cell collection apparatus |
| US6828098B2 (en) | 2000-05-20 | 2004-12-07 | The Regents Of The University Of Michigan | Method of producing a DNA library using positional amplification based on the use of adaptors and nick translation |
| AU2001267191A1 (en) | 2000-06-06 | 2001-12-17 | Tm Bioscience Corporation | Capture moieties for nucleic acids and uses thereof |
| US7439016B1 (en) | 2000-06-15 | 2008-10-21 | Digene Corporation | Detection of nucleic acids by type-specific hybrid capture method |
| US7601497B2 (en) | 2000-06-15 | 2009-10-13 | Qiagen Gaithersburg, Inc. | Detection of nucleic acids by target-specific hybrid capture method |
| AU2002215488B8 (en) | 2000-06-21 | 2007-11-15 | Qiagen Gaithersburg, Inc. | Universal collection medium |
| AU2001279795A1 (en) * | 2000-08-01 | 2002-02-13 | Mirko Essling | Measuring device and measuring method for determining distance and/or position |
| US6803200B2 (en) | 2000-12-12 | 2004-10-12 | Invitrogen Corporation | Compositions and methods for the release of nucleic acid molecules from solid matrices |
| ES2322841T3 (es) | 2001-02-16 | 2009-06-30 | Promega Corporation | Aislamiento y purificacion magnetica de acidos nucleicos. |
| US6542856B2 (en) * | 2001-06-15 | 2003-04-01 | General Electric Company | System and method for monitoring gas turbine plants |
| AU2002315397A1 (en) * | 2001-06-20 | 2003-01-08 | Power Medical Interventions, Inc. | A method and system for integrated medical tracking |
| EP1806410A3 (en) | 2001-08-23 | 2007-10-24 | Merck & Co., Inc. | Fluorescent multiplex HPV PCR assays using multiple fluorophores |
| WO2003027242A2 (en) * | 2001-09-24 | 2003-04-03 | Duke University | Fas ligand |
| LU90840B1 (en) * | 2001-09-25 | 2003-03-26 | Delphi Tech Inc | Method for controlling the operation of a system sub-system or component |
| US20050032105A1 (en) | 2001-10-12 | 2005-02-10 | Bair Robert Jackson | Compositions and methods for using a solid support to purify DNA |
| US6977148B2 (en) | 2001-10-15 | 2005-12-20 | Qiagen Gmbh | Multiple displacement amplification |
| ES2654909T3 (es) | 2002-01-07 | 2018-02-15 | Pretect As | Método para detectar ARNm del virus del papiloma humano |
| JP4521152B2 (ja) * | 2002-03-05 | 2010-08-11 | 株式会社東芝 | 半導体製造装置 |
| US20030175828A1 (en) | 2002-03-15 | 2003-09-18 | Lazar James G. | Signal amplification by Hybrid Capture |
| US20030208196A1 (en) * | 2002-05-03 | 2003-11-06 | Arthrocare Corporation | Control system for limited-use device |
| US7648678B2 (en) * | 2002-12-20 | 2010-01-19 | Dako Denmark A/S | Method and system for pretreatment of tissue slides |
| WO2005003375A2 (en) * | 2003-01-29 | 2005-01-13 | 454 Corporation | Methods of amplifying and sequencing nucleic acids |
| ATE550099T1 (de) | 2003-04-04 | 2012-04-15 | Hoffmann La Roche | Verbessertes system für mehrfarbige echtzeit-pcr |
| US7129260B2 (en) * | 2003-06-02 | 2006-10-31 | Abbott Laboratories | Isoindolinone kinase inhibitors |
| EP1641809B2 (en) | 2003-07-05 | 2018-10-03 | The Johns Hopkins University | Method and compositions for detection and enumeration of genetic variations |
| DE60300339T2 (de) * | 2003-08-25 | 2006-04-13 | Mtm Laboratories Ag | Verfahren zum Nachweis von Karzinomen in solubilisierten zervikalen Körperproben |
| WO2005033333A2 (en) | 2003-10-07 | 2005-04-14 | Dako Denmark A/S | Methods and compositions for the diagnosis of cancer |
| US8012944B2 (en) * | 2003-10-30 | 2011-09-06 | Pharmascience Inc. | Method for treating cancer using IAP antisense oligomer and chemotherapeutic agent |
| US20070083286A1 (en) * | 2004-03-09 | 2007-04-12 | Olympus Corporation | Medical instrument and equipment for managing of the same |
| CN1690223B (zh) | 2004-04-27 | 2010-05-05 | 亚能生物技术（深圳）有限公司 | 人乳头瘤病毒分型基因芯片检测系统 |
| US7398181B2 (en) * | 2004-06-02 | 2008-07-08 | Hewlett-Packard Development Company, L.P. | Method for retrieving reliability data in a system |
| US20060095096A1 (en) * | 2004-09-09 | 2006-05-04 | Debenedictis Leonard C | Interchangeable tips for medical laser treatments and methods for using same |
| EP2458015B1 (en) | 2004-09-30 | 2015-12-23 | Epigenomics AG | Method for providing DNA fragments derived from an archived sample |
| AU2005302453A1 (en) | 2004-10-29 | 2006-05-11 | Medimmune, Llc | Methods of preventing and treating RSV infections and related conditions |
| US20060129269A1 (en) * | 2004-12-15 | 2006-06-15 | Xerox Corporation | Processes for using a memory storage device in conjunction with tooling |
| CN101151379A (zh) * | 2005-01-14 | 2008-03-26 | 密执安州立大学董事会 | 检测生物样品中的人乳头瘤病毒的系统、方法和组合物 |
| US20060240449A1 (en) | 2005-01-19 | 2006-10-26 | Mcglennen Ronald C | Methods and compositions for preparation of biological samples |
| CA2602185A1 (en) | 2005-04-06 | 2006-10-06 | Verenium Corporation | Enzymes and formulations for broad-specificity decontamination of chemical and biological warfare agents |
| US7972776B2 (en) | 2005-11-15 | 2011-07-05 | Oncohealth Corporation | Protein chips for HPV detection |
| US20080003565A1 (en) | 2006-05-02 | 2008-01-03 | Government Of The Us, As Represented By The Secretary, Department Of Health And Human Services | Viral nucleic acid microarray and method of use |
| US8962262B2 (en) | 2006-05-11 | 2015-02-24 | Arbor Vita Corporation | Method of protein extraction from cells |
| ATE531725T1 (de) | 2007-02-27 | 2011-11-15 | Nuclea Biomarkers Llc | Verfahren zur vorhersage ob nsclc-patienten auf eine behandlung mit einem egfr-tk-hemmer ansprechen |
| US8357538B2 (en) | 2007-04-06 | 2013-01-22 | Qiagen Gaithersburg, Inc. | Automated assay and system |
| US7985375B2 (en) | 2007-04-06 | 2011-07-26 | Qiagen Gaithersburg, Inc. | Sample preparation system and method for processing clinical specimens |
| US8703492B2 (en) | 2007-04-06 | 2014-04-22 | Qiagen Gaithersburg, Inc. | Open platform hybrid manual-automated sample processing system |
| WO2008139938A1 (ja) | 2007-05-02 | 2008-11-20 | National University Corporation Tokyo Medical And Dental University | ヒトパピローマウイルス１６型遺伝子を標的とする二本鎖核酸分子及びそれを含む医薬 |
| US8623599B2 (en) | 2007-06-08 | 2014-01-07 | Epigenomics Ag | Method for methylation analysis |
| CN101177701B (zh) | 2007-09-29 | 2010-08-18 | 潮州凯普生物化学有限公司 | 人乳头状瘤病毒基因分型检测试剂盒及其基因芯片制备方法 |
| US20100285471A1 (en) | 2007-10-11 | 2010-11-11 | The Ohio State University Research Foundation | Methods and Compositions for the Diagnosis and Treatment of Esphageal Adenocarcinomas |
| JP2009106220A (ja) | 2007-10-31 | 2009-05-21 | Genodive Pharma Kk | 基板上での等温増幅反応による標的塩基配列の捕捉及び検出方法 |
| WO2009057993A1 (en) | 2007-11-01 | 2009-05-07 | Vereniging Voor Christelijk Hoger Onderwijs, Wetenschappelijk Onderzoek En Patiëntenzorg | NEW DETECTION METHOD FOR CERVICAL HPVs |
| US20110027798A1 (en) | 2008-03-31 | 2011-02-03 | The Ohio State University Research Foundation | Hybridization Quantitation Method for Modified Micro-RNA and -DNA Based Oligonucleotides |
| JP2011518333A (ja) * | 2008-04-17 | 2011-06-23 | キアジェン ゲイサーズバーグ インコーポレイテッド | 標的核酸の存在を判別するための合成プローブを用いた組成物、方法、およびキット |
| WO2010004251A1 (en) | 2008-06-16 | 2010-01-14 | Oncomethylome Sciences Sa | Dna methylomes |
| WO2010028382A2 (en) | 2008-09-05 | 2010-03-11 | Mayo Foundation For Medical Education And Research | Collecting and processing complex macromolecular mixtures |
| US9090948B2 (en) | 2008-09-30 | 2015-07-28 | Abbott Molecular Inc. | Primers and probes for detecting human papillomavirus and human beta globin sequences in test samples |
| WO2010062546A1 (en) | 2008-10-27 | 2010-06-03 | Qiagen Gaithersburg Inc. | Fast results hybrid capture assay on an automated platform |
| EP2184368A1 (en) | 2008-11-07 | 2010-05-12 | DKFZ Deutsches Krebsforschungszentrum | Diagnostic transcript and splice patterns of HPV16 in different cervical lesions |
| AU2010208386B2 (en) * | 2009-01-27 | 2016-08-11 | Qiagen Gaithersburg | Thermophilic helicase dependent amplification technology with endpoint homogenous fluorescent detection |
| JP6108661B2 (ja) * | 2009-01-28 | 2017-04-05 | キアジェン ゲイサーズバーグ インコーポレイテッド | 配列特異的な大量試料調製方法およびアッセイ法 |
| EP2425019B1 (en) | 2009-05-01 | 2014-03-19 | QIAGEN Gaithersburg, Inc. | A non-target amplification method for detection of rna splice-forms in a sample |
| US20110053143A1 (en) * | 2009-09-03 | 2011-03-03 | Qiagen Gaithersburg Inc. | Methods and kits for determining viral load in clinical samples |
| JP5826752B2 (ja) * | 2009-09-14 | 2015-12-02 | キアジェン ゲイサーズバーグ インコーポレイテッド | 細胞学用培地に固定された組織サンプルから核酸またはタンパク質を回収するための組成物および方法 |
| BR112012016451A2 (pt) * | 2010-01-04 | 2020-09-08 | Giagen Gaithersburg, INC. | métodos, composições, e kits para recuperação de ácidos nucleicos ou proteínas de amostras de tecido fixadas |
| CA2786473A1 (en) * | 2010-01-08 | 2011-07-14 | Qiagen Gaithersburg, Inc. | Materials and methods for isothermal nucleic acid amplification |
| CA2787781A1 (en) * | 2010-01-29 | 2011-08-04 | Qiagen Gaithersburg, Inc. | Method of determining and confirming the presence of an hpv in a sample |
| CA2787924A1 (en) * | 2010-01-29 | 2011-08-04 | Qiagen Gaithersburg, Inc. | Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids |
| JP2013528049A (ja) * | 2010-05-19 | 2013-07-08 | キアゲン ガイサーズバーグ アイエヌシー． | 核酸の配列特異的な精製及び多重分析のための方法及び組成物 |
| JP6153866B2 (ja) * | 2010-05-25 | 2017-06-28 | キアゲン ガイサーズバーグ アイエヌシー． | 迅速なハイブリッド捕捉アッセイ、及び関連する戦略的に切断されたプローブ |
-
2009
- 2009-10-26 WO PCT/US2009/062041 patent/WO2010062546A1/en not_active Ceased
- 2009-10-26 ES ES09752940.8T patent/ES2622062T3/es active Active
- 2009-10-26 WO PCT/US2009/062061 patent/WO2010062556A1/en not_active Ceased
- 2009-10-26 CN CN2009801436829A patent/CN102203293A/zh active Pending
- 2009-10-26 TW TW098136140A patent/TWI394839B/zh not_active IP Right Cessation
- 2009-10-26 TW TW102108255A patent/TWI419974B/zh not_active IP Right Cessation
- 2009-10-26 CA CA2741650A patent/CA2741650C/en not_active Expired - Fee Related
- 2009-10-26 EP EP09752940.8A patent/EP2347011B1/en not_active Not-in-force
- 2009-10-26 AU AU2009320213A patent/AU2009320213B2/en not_active Ceased
- 2009-10-26 JP JP2011533405A patent/JP2012506705A/ja active Pending
- 2009-10-26 TW TW098136144A patent/TWI417389B/zh not_active IP Right Cessation
- 2009-10-26 US US12/605,540 patent/US8288520B2/en not_active Expired - Fee Related
- 2009-10-26 US US12/605,605 patent/US8877436B2/en not_active Expired - Fee Related
-
2012
- 2012-09-14 US US13/616,899 patent/US8735564B2/en not_active Expired - Fee Related
-
2015
- 2015-02-02 JP JP2015018291A patent/JP2015119719A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| AU2009320213A1 (en) | 2010-06-03 |
| WO2010062546A1 (en) | 2010-06-03 |
| US8288520B2 (en) | 2012-10-16 |
| US8877436B2 (en) | 2014-11-04 |
| TWI417389B (zh) | 2013-12-01 |
| US20100159463A1 (en) | 2010-06-24 |
| TW201323616A (zh) | 2013-06-16 |
| WO2010062556A1 (en) | 2010-06-03 |
| EP2347011B1 (en) | 2017-01-18 |
| ES2622062T3 (es) | 2017-07-05 |
| TWI419974B (zh) | 2013-12-21 |
| US20100105060A1 (en) | 2010-04-29 |
| EP2347011A1 (en) | 2011-07-27 |
| JP2012506705A (ja) | 2012-03-22 |
| CA2741650A1 (en) | 2010-06-03 |
| TW201022448A (en) | 2010-06-16 |
| TW201022447A (en) | 2010-06-16 |
| JP2015119719A (ja) | 2015-07-02 |
| AU2009320213B2 (en) | 2016-06-16 |
| US20130011830A1 (en) | 2013-01-10 |
| TWI394839B (zh) | 2013-05-01 |
| CN102203293A (zh) | 2011-09-28 |
| US8735564B2 (en) | 2014-05-27 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CA2741650C (en) | Fast results hybrid capture assay and system | |
| JP2012506705A5 (https=) | ||
| JP2011518333A (ja) | 標的核酸の存在を判別するための合成プローブを用いた組成物、方法、およびキット | |
| US9605303B2 (en) | Method of determining and confirming the presence of an HPV in a sample | |
| US9376727B2 (en) | Fast results hybrid capture assay and associated strategically truncated probes | |
| AU2010208311B2 (en) | Sequence-specific large volume sample preparation method and assay |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request |
Effective date: 20140926 |
|
| MKLA | Lapsed |
Effective date: 20191028 |