CA2706224A1 - Transgenic chickens comprising an inactivated endogenous immunoglobulin light chain gene and methods to produce such chickens using genetically modified primordial germ cells - Google Patents

Transgenic chickens comprising an inactivated endogenous immunoglobulin light chain gene and methods to produce such chickens using genetically modified primordial germ cells Download PDF

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Publication number
CA2706224A1
CA2706224A1 CA2706224A CA2706224A CA2706224A1 CA 2706224 A1 CA2706224 A1 CA 2706224A1 CA 2706224 A CA2706224 A CA 2706224A CA 2706224 A CA2706224 A CA 2706224A CA 2706224 A1 CA2706224 A1 CA 2706224A1
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CA
Canada
Prior art keywords
gene
cells
pgcs
chicken
transgene
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA2706224A
Other languages
English (en)
French (fr)
Inventor
Marie-Cecile Van De Lavoir
Philip Albert Leighton
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Synageva Biopharma Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2706224A1 publication Critical patent/CA2706224A1/en
Abandoned legal-status Critical Current

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Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027New or modified breeds of vertebrates
    • A01K67/0275Genetically modified vertebrates, e.g. transgenic
    • A01K67/0276Knock-out vertebrates
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/465Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from birds
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K16/00Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90Stable introduction of foreign DNA into chromosome
    • C12N15/902Stable introduction of foreign DNA into chromosome using homologous recombination
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/05Animals comprising random inserted nucleic acids (transgenic)
    • A01K2217/052Animals comprising random inserted nucleic acids (transgenic) inducing gain of function
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/07Animals genetically altered by homologous recombination
    • A01K2217/075Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00Animals characterised by species
    • A01K2227/30Bird
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00Animals characterised by purpose
    • A01K2267/01Animal expressing industrially exogenous proteins
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00Animals characterised by purpose
    • A01K2267/03Animal model, e.g. for test or diseases
    • A01K2267/0393Animal model comprising a reporter system for screening tests
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00Nucleic acids vectors
    • C12N2800/30Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Environmental Sciences (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Veterinary Medicine (AREA)
  • Physics & Mathematics (AREA)
  • Animal Husbandry (AREA)
  • Immunology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Toxicology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA2706224A 2007-08-14 2008-08-14 Transgenic chickens comprising an inactivated endogenous immunoglobulin light chain gene and methods to produce such chickens using genetically modified primordial germ cells Abandoned CA2706224A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US96489107P 2007-08-14 2007-08-14
US60/964,891 2007-08-14
PCT/US2008/073214 WO2009023800A1 (en) 2007-08-14 2008-08-14 Transgenic chickens with an inactivated endogenous gene locus

Publications (1)

Publication Number Publication Date
CA2706224A1 true CA2706224A1 (en) 2009-02-19

Family

ID=40351167

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2706224A Abandoned CA2706224A1 (en) 2007-08-14 2008-08-14 Transgenic chickens comprising an inactivated endogenous immunoglobulin light chain gene and methods to produce such chickens using genetically modified primordial germ cells

Country Status (7)

Country Link
EP (1) EP2187732A4 (ko)
JP (3) JP5760159B2 (ko)
KR (1) KR101563017B1 (ko)
CN (1) CN101868144A (ko)
AU (1) AU2008286791A1 (ko)
CA (1) CA2706224A1 (ko)
WO (1) WO2009023800A1 (ko)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2835222C (en) 2011-05-24 2020-08-25 Crystal Bioscience Inc. Transgenic chicken comprising an inactivated immunoglobulin gene
AU2013204327B2 (en) * 2012-04-20 2016-09-01 Aviagen Cell transfection method
US11033009B2 (en) * 2015-08-27 2021-06-15 Crystal Bioscience Inc. Transgenic chicken for production of antibodies having a common light chain
JP2020536580A (ja) * 2017-09-19 2020-12-17 ザ ステート オブ イスラエル, ミニストリー オブ アグリカルチャー アンド ルーラル ディヴェロプメント, アグリカルチュラル リサーチ オーガニゼーション (エーアールオー) (ボルカニ センター) ゲノム編集された鳥
CN110484549B (zh) * 2018-04-20 2023-10-03 北京大学 基因组靶向修饰方法
KR20210137499A (ko) * 2019-03-05 2021-11-17 더 스테이트 오브 이스라엘, 미니스트리 오브 애그리컬처 & 루럴 디벨로프먼트, 애그리컬처럴 리서치 오거니제이션, (에이.알.오.), 볼카니 센터 게놈-편집 조류
WO2023127903A1 (ja) * 2021-12-28 2023-07-06 国立大学法人北海道大学 始原生殖細胞が富化された胚を調製する方法
CN115120382A (zh) * 2022-05-26 2022-09-30 广东省农业科学院动物科学研究所 一种鸡生殖前体细胞移植受体的制备方法
CN115191404B (zh) * 2022-07-28 2023-04-21 四川省畜牧科学研究院 提高黄羽肉鸡后代公鸡比例的新品种选育方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE489461T1 (de) * 1997-10-16 2010-12-15 Univ Georgia Transgene vögel und proteinproduktion
GB2398784B (en) * 2003-02-26 2005-07-27 Babraham Inst Removal and modification of the immunoglobulin constant region gene cluster of a non-human mammal
WO2005007696A2 (en) * 2003-07-15 2005-01-27 Therapeutic Human Polyclonals, Inc. Humanized immunoglobulin loci
AU2006210645A1 (en) * 2005-02-01 2006-08-10 Symphogen A/S Transgenic chickens

Also Published As

Publication number Publication date
JP2010536346A (ja) 2010-12-02
EP2187732A4 (en) 2012-11-07
JP5760159B2 (ja) 2015-08-05
EP2187732A1 (en) 2010-05-26
AU2008286791A1 (en) 2009-02-19
WO2009023800A1 (en) 2009-02-19
CN101868144A (zh) 2010-10-20
KR101563017B1 (ko) 2015-10-23
JP2014239702A (ja) 2014-12-25
JP2016129519A (ja) 2016-07-21
KR20100088125A (ko) 2010-08-06
JP6004290B2 (ja) 2016-10-05

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Legal Events

Date Code Title Description
EEER Examination request

Effective date: 20130404

FZDE Discontinued

Effective date: 20180628