AU2008286791A1 - Transgenic chickens with an inactivated endogenous gene locus - Google Patents
Transgenic chickens with an inactivated endogenous gene locus Download PDFInfo
- Publication number
- AU2008286791A1 AU2008286791A1 AU2008286791A AU2008286791A AU2008286791A1 AU 2008286791 A1 AU2008286791 A1 AU 2008286791A1 AU 2008286791 A AU2008286791 A AU 2008286791A AU 2008286791 A AU2008286791 A AU 2008286791A AU 2008286791 A1 AU2008286791 A1 AU 2008286791A1
- Authority
- AU
- Australia
- Prior art keywords
- gene
- cells
- chicken
- endogenous
- pgcs
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 108090000623 proteins and genes Proteins 0.000 title claims description 227
- 241000287828 Gallus gallus Species 0.000 title claims description 216
- 230000009261 transgenic effect Effects 0.000 title claims description 104
- 235000013330 chicken meat Nutrition 0.000 title description 141
- 210000004027 cell Anatomy 0.000 claims description 322
- 210000004602 germ cell Anatomy 0.000 claims description 162
- 230000008685 targeting Effects 0.000 claims description 54
- 238000000034 method Methods 0.000 claims description 44
- 210000001161 mammalian embryo Anatomy 0.000 claims description 40
- 239000003550 marker Substances 0.000 claims description 38
- 230000010354 integration Effects 0.000 claims description 32
- 238000012217 deletion Methods 0.000 claims description 18
- 230000037430 deletion Effects 0.000 claims description 18
- 108700005091 Immunoglobulin Genes Proteins 0.000 claims description 10
- 108060003951 Immunoglobulin Proteins 0.000 claims description 5
- 108010065825 Immunoglobulin Light Chains Proteins 0.000 claims description 5
- 102000018358 immunoglobulin Human genes 0.000 claims description 5
- 108020004705 Codon Proteins 0.000 claims description 4
- 230000012447 hatching Effects 0.000 claims description 4
- 108020004485 Nonsense Codon Proteins 0.000 claims description 3
- 238000001514 detection method Methods 0.000 claims description 3
- 101150111062 C gene Proteins 0.000 claims 4
- 108010019476 Immunoglobulin Heavy Chains Proteins 0.000 claims 4
- 101150008942 J gene Proteins 0.000 claims 4
- 101150117115 V gene Proteins 0.000 claims 4
- 102000006496 Immunoglobulin Heavy Chains Human genes 0.000 claims 2
- 102000013463 Immunoglobulin Light Chains Human genes 0.000 claims 1
- 108700019146 Transgenes Proteins 0.000 description 196
- 108020004414 DNA Proteins 0.000 description 109
- 238000003780 insertion Methods 0.000 description 88
- 230000037431 insertion Effects 0.000 description 88
- 239000012634 fragment Substances 0.000 description 86
- 210000002257 embryonic structure Anatomy 0.000 description 80
- 230000014509 gene expression Effects 0.000 description 79
- 101150036876 cre gene Proteins 0.000 description 71
- 230000006798 recombination Effects 0.000 description 67
- 238000005215 recombination Methods 0.000 description 67
- 239000013598 vector Substances 0.000 description 62
- 210000001519 tissue Anatomy 0.000 description 57
- 241000271566 Aves Species 0.000 description 48
- 241001465754 Metazoa Species 0.000 description 46
- 239000013612 plasmid Substances 0.000 description 46
- 102000004169 proteins and genes Human genes 0.000 description 44
- RXWNCPJZOCPEPQ-NVWDDTSBSA-N puromycin Chemical compound C1=CC(OC)=CC=C1C[C@H](N)C(=O)N[C@H]1[C@@H](O)[C@H](N2C3=NC=NC(=C3N=C2)N(C)C)O[C@@H]1CO RXWNCPJZOCPEPQ-NVWDDTSBSA-N 0.000 description 44
- 101000607560 Homo sapiens Ubiquitin-conjugating enzyme E2 variant 3 Proteins 0.000 description 42
- 102100039936 Ubiquitin-conjugating enzyme E2 variant 3 Human genes 0.000 description 42
- 102100034343 Integrase Human genes 0.000 description 41
- 108010061833 Integrases Proteins 0.000 description 41
- 238000001890 transfection Methods 0.000 description 39
- 238000003032 molecular docking Methods 0.000 description 38
- 230000005540 biological transmission Effects 0.000 description 36
- 210000000349 chromosome Anatomy 0.000 description 31
- 238000012239 gene modification Methods 0.000 description 30
- 238000004458 analytical method Methods 0.000 description 29
- 238000004519 manufacturing process Methods 0.000 description 26
- 230000001404 mediated effect Effects 0.000 description 26
- 239000000047 product Substances 0.000 description 26
- 239000000523 sample Substances 0.000 description 26
- 230000005017 genetic modification Effects 0.000 description 25
- 235000013617 genetically modified food Nutrition 0.000 description 25
- 101710098213 Beta-1,3-glucan-binding protein Proteins 0.000 description 23
- 238000011161 development Methods 0.000 description 23
- 230000018109 developmental process Effects 0.000 description 23
- 238000002105 Southern blotting Methods 0.000 description 22
- 230000002779 inactivation Effects 0.000 description 22
- 108010051219 Cre recombinase Proteins 0.000 description 21
- 229950010131 puromycin Drugs 0.000 description 21
- 108010048367 enhanced green fluorescent protein Proteins 0.000 description 20
- 238000004113 cell culture Methods 0.000 description 19
- 230000000694 effects Effects 0.000 description 19
- 229930193140 Neomycin Natural products 0.000 description 18
- 239000012212 insulator Substances 0.000 description 18
- 229960004927 neomycin Drugs 0.000 description 18
- 230000029087 digestion Effects 0.000 description 17
- 230000007774 longterm Effects 0.000 description 17
- 210000001550 testis Anatomy 0.000 description 17
- 241000699666 Mus <mouse, genus> Species 0.000 description 16
- 238000009395 breeding Methods 0.000 description 15
- 230000001488 breeding effect Effects 0.000 description 15
- 235000013601 eggs Nutrition 0.000 description 15
- 230000002068 genetic effect Effects 0.000 description 15
- 230000001568 sexual effect Effects 0.000 description 15
- 230000000392 somatic effect Effects 0.000 description 15
- 230000006870 function Effects 0.000 description 14
- 241000699670 Mus sp. Species 0.000 description 13
- 238000004520 electroporation Methods 0.000 description 13
- 108700028369 Alleles Proteins 0.000 description 12
- 239000012528 membrane Substances 0.000 description 12
- 239000004677 Nylon Substances 0.000 description 11
- 238000002744 homologous recombination Methods 0.000 description 11
- 230000006801 homologous recombination Effects 0.000 description 11
- 229920001778 nylon Polymers 0.000 description 11
- 239000011435 rock Substances 0.000 description 11
- 108091060211 Expressed sequence tag Proteins 0.000 description 10
- 241000713666 Lentivirus Species 0.000 description 10
- 229930189065 blasticidin Natural products 0.000 description 10
- 230000001105 regulatory effect Effects 0.000 description 10
- 210000005166 vasculature Anatomy 0.000 description 10
- 239000011543 agarose gel Substances 0.000 description 9
- 210000004369 blood Anatomy 0.000 description 9
- 239000008280 blood Substances 0.000 description 9
- 229940079593 drug Drugs 0.000 description 9
- 239000003814 drug Substances 0.000 description 9
- 210000001671 embryonic stem cell Anatomy 0.000 description 9
- 238000005516 engineering process Methods 0.000 description 9
- 210000002149 gonad Anatomy 0.000 description 9
- 239000002773 nucleotide Substances 0.000 description 9
- 125000003729 nucleotide group Chemical group 0.000 description 9
- 108010085238 Actins Proteins 0.000 description 8
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 8
- 108091092195 Intron Proteins 0.000 description 8
- 238000013459 approach Methods 0.000 description 8
- 230000003115 biocidal effect Effects 0.000 description 8
- 210000004556 brain Anatomy 0.000 description 8
- 239000000872 buffer Substances 0.000 description 8
- 238000006243 chemical reaction Methods 0.000 description 8
- 239000002299 complementary DNA Substances 0.000 description 8
- 239000002609 medium Substances 0.000 description 8
- 108020004999 messenger RNA Proteins 0.000 description 8
- 108091008146 restriction endonucleases Proteins 0.000 description 8
- 210000000582 semen Anatomy 0.000 description 8
- 241000894007 species Species 0.000 description 8
- 238000012546 transfer Methods 0.000 description 8
- 238000011144 upstream manufacturing Methods 0.000 description 8
- 230000003612 virological effect Effects 0.000 description 8
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 7
- 102000007469 Actins Human genes 0.000 description 7
- 102100026608 Aldehyde dehydrogenase family 3 member A2 Human genes 0.000 description 7
- 108091029523 CpG island Proteins 0.000 description 7
- 101150090523 DAZL gene Proteins 0.000 description 7
- 102100028572 Disabled homolog 2 Human genes 0.000 description 7
- 101000717967 Homo sapiens Aldehyde dehydrogenase family 3 member A2 Proteins 0.000 description 7
- 101000866272 Homo sapiens Double C2-like domain-containing protein alpha Proteins 0.000 description 7
- 238000009795 derivation Methods 0.000 description 7
- 238000010586 diagram Methods 0.000 description 7
- 230000035558 fertility Effects 0.000 description 7
- 230000004048 modification Effects 0.000 description 7
- 238000012986 modification Methods 0.000 description 7
- 230000002829 reductive effect Effects 0.000 description 7
- 238000003757 reverse transcription PCR Methods 0.000 description 7
- 230000001225 therapeutic effect Effects 0.000 description 7
- 238000013518 transcription Methods 0.000 description 7
- 230000035897 transcription Effects 0.000 description 7
- 108010077544 Chromatin Proteins 0.000 description 6
- 108091029865 Exogenous DNA Proteins 0.000 description 6
- 108010017842 Telomerase Proteins 0.000 description 6
- 230000003321 amplification Effects 0.000 description 6
- 210000003483 chromatin Anatomy 0.000 description 6
- 230000007547 defect Effects 0.000 description 6
- 238000010363 gene targeting Methods 0.000 description 6
- 230000012010 growth Effects 0.000 description 6
- 239000001963 growth medium Substances 0.000 description 6
- 238000002955 isolation Methods 0.000 description 6
- 239000000203 mixture Substances 0.000 description 6
- 238000003199 nucleic acid amplification method Methods 0.000 description 6
- 210000003101 oviduct Anatomy 0.000 description 6
- 230000002062 proliferating effect Effects 0.000 description 6
- 210000002863 seminiferous tubule Anatomy 0.000 description 6
- 239000000126 substance Substances 0.000 description 6
- FWBHETKCLVMNFS-UHFFFAOYSA-N 4',6-Diamino-2-phenylindol Chemical compound C1=CC(C(=N)N)=CC=C1C1=CC2=CC=C(C(N)=N)C=C2N1 FWBHETKCLVMNFS-UHFFFAOYSA-N 0.000 description 5
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 5
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 5
- 206010028980 Neoplasm Diseases 0.000 description 5
- 241000700605 Viruses Species 0.000 description 5
- 230000002759 chromosomal effect Effects 0.000 description 5
- 239000003623 enhancer Substances 0.000 description 5
- 239000012091 fetal bovine serum Substances 0.000 description 5
- 238000000338 in vitro Methods 0.000 description 5
- 238000011534 incubation Methods 0.000 description 5
- 230000035772 mutation Effects 0.000 description 5
- 239000013642 negative control Substances 0.000 description 5
- 230000001177 retroviral effect Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 108020002663 Aldehyde Dehydrogenase Proteins 0.000 description 4
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 4
- 101100240528 Caenorhabditis elegans nhr-23 gene Proteins 0.000 description 4
- 102100035353 Cyclin-dependent kinase 2-associated protein 1 Human genes 0.000 description 4
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 4
- 102000004190 Enzymes Human genes 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 4
- 238000012413 Fluorescence activated cell sorting analysis Methods 0.000 description 4
- 108091028043 Nucleic acid sequence Proteins 0.000 description 4
- 108010052160 Site-specific recombinase Proteins 0.000 description 4
- 241000251539 Vertebrata <Metazoa> Species 0.000 description 4
- 208000008383 Wilms tumor Diseases 0.000 description 4
- 238000003556 assay Methods 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 238000010367 cloning Methods 0.000 description 4
- 238000013461 design Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000013604 expression vector Substances 0.000 description 4
- 239000000499 gel Substances 0.000 description 4
- 210000001654 germ layer Anatomy 0.000 description 4
- 238000009396 hybridization Methods 0.000 description 4
- NOESYZHRGYRDHS-UHFFFAOYSA-N insulin Chemical compound N1C(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(NC(=O)CN)C(C)CC)CSSCC(C(NC(CO)C(=O)NC(CC(C)C)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CCC(N)=O)C(=O)NC(CC(C)C)C(=O)NC(CCC(O)=O)C(=O)NC(CC(N)=O)C(=O)NC(CC=2C=CC(O)=CC=2)C(=O)NC(CSSCC(NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2C=CC(O)=CC=2)NC(=O)C(CC(C)C)NC(=O)C(C)NC(=O)C(CCC(O)=O)NC(=O)C(C(C)C)NC(=O)C(CC(C)C)NC(=O)C(CC=2NC=NC=2)NC(=O)C(CO)NC(=O)CNC2=O)C(=O)NCC(=O)NC(CCC(O)=O)C(=O)NC(CCCNC(N)=N)C(=O)NCC(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC=CC=3)C(=O)NC(CC=3C=CC(O)=CC=3)C(=O)NC(C(C)O)C(=O)N3C(CCC3)C(=O)NC(CCCCN)C(=O)NC(C)C(O)=O)C(=O)NC(CC(N)=O)C(O)=O)=O)NC(=O)C(C(C)CC)NC(=O)C(CO)NC(=O)C(C(C)O)NC(=O)C1CSSCC2NC(=O)C(CC(C)C)NC(=O)C(NC(=O)C(CCC(N)=O)NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(N)CC=1C=CC=CC=1)C(C)C)CC1=CN=CN1 NOESYZHRGYRDHS-UHFFFAOYSA-N 0.000 description 4
- 210000004185 liver Anatomy 0.000 description 4
- 230000007246 mechanism Effects 0.000 description 4
- 210000003205 muscle Anatomy 0.000 description 4
- 230000001537 neural effect Effects 0.000 description 4
- 239000013641 positive control Substances 0.000 description 4
- 244000144977 poultry Species 0.000 description 4
- 235000013594 poultry meat Nutrition 0.000 description 4
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000012216 screening Methods 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 230000035899 viability Effects 0.000 description 4
- 108020005345 3' Untranslated Regions Proteins 0.000 description 3
- 241000283690 Bos taurus Species 0.000 description 3
- 208000031639 Chromosome Deletion Diseases 0.000 description 3
- 108091035707 Consensus sequence Proteins 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 102000002322 Egg Proteins Human genes 0.000 description 3
- 108010000912 Egg Proteins Proteins 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- 241000286209 Phasianidae Species 0.000 description 3
- 241000700159 Rattus Species 0.000 description 3
- 150000001299 aldehydes Chemical class 0.000 description 3
- 230000010056 antibody-dependent cellular cytotoxicity Effects 0.000 description 3
- 230000006907 apoptotic process Effects 0.000 description 3
- 239000003636 conditioned culture medium Substances 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 210000000969 egg white Anatomy 0.000 description 3
- 235000014103 egg white Nutrition 0.000 description 3
- 230000001747 exhibiting effect Effects 0.000 description 3
- 210000003746 feather Anatomy 0.000 description 3
- 230000007045 gastrulation Effects 0.000 description 3
- 230000030279 gene silencing Effects 0.000 description 3
- 238000010353 genetic engineering Methods 0.000 description 3
- 210000005260 human cell Anatomy 0.000 description 3
- 230000016784 immunoglobulin production Effects 0.000 description 3
- 230000006698 induction Effects 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- 208000021267 infertility disease Diseases 0.000 description 3
- 238000005304 joining Methods 0.000 description 3
- 231100000518 lethal Toxicity 0.000 description 3
- 230000001665 lethal effect Effects 0.000 description 3
- 230000000877 morphologic effect Effects 0.000 description 3
- 239000002243 precursor Substances 0.000 description 3
- 230000035755 proliferation Effects 0.000 description 3
- 230000008707 rearrangement Effects 0.000 description 3
- 230000003252 repetitive effect Effects 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
- 210000002027 skeletal muscle Anatomy 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000003153 stable transfection Methods 0.000 description 3
- 230000010474 transient expression Effects 0.000 description 3
- 239000013603 viral vector Substances 0.000 description 3
- 101150029062 15 gene Proteins 0.000 description 2
- 101150098072 20 gene Proteins 0.000 description 2
- QCVGEOXPDFCNHA-UHFFFAOYSA-N 5,5-dimethyl-2,4-dioxo-1,3-oxazolidine-3-carboxamide Chemical compound CC1(C)OC(=O)N(C(N)=O)C1=O QCVGEOXPDFCNHA-UHFFFAOYSA-N 0.000 description 2
- 102000005369 Aldehyde Dehydrogenase Human genes 0.000 description 2
- 241000269332 Ambystoma mexicanum Species 0.000 description 2
- 241001244729 Apalis Species 0.000 description 2
- COVZYZSDYWQREU-UHFFFAOYSA-N Busulfan Chemical compound CS(=O)(=O)OCCCCOS(C)(=O)=O COVZYZSDYWQREU-UHFFFAOYSA-N 0.000 description 2
- 206010059866 Drug resistance Diseases 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 108010046276 FLP recombinase Proteins 0.000 description 2
- 101100231735 Gallus gallus HPRT1 gene Proteins 0.000 description 2
- 208000034951 Genetic Translocation Diseases 0.000 description 2
- 241000282412 Homo Species 0.000 description 2
- 102000004877 Insulin Human genes 0.000 description 2
- 108090001061 Insulin Proteins 0.000 description 2
- 108010001127 Insulin Receptor Proteins 0.000 description 2
- 108010075869 Isocitrate Dehydrogenase Proteins 0.000 description 2
- 102000012011 Isocitrate Dehydrogenase Human genes 0.000 description 2
- 108010025815 Kanamycin Kinase Proteins 0.000 description 2
- 241001529936 Murinae Species 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 2
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 2
- 102100026940 Small ubiquitin-related modifier 1 Human genes 0.000 description 2
- 102100029462 Sodium-dependent lysophosphatidylcholine symporter 1 Human genes 0.000 description 2
- 101710185583 Sodium-dependent lysophosphatidylcholine symporter 1 Proteins 0.000 description 2
- 210000001744 T-lymphocyte Anatomy 0.000 description 2
- 108700009124 Transcription Initiation Site Proteins 0.000 description 2
- 241000269370 Xenopus <genus> Species 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- 102000006646 aminoglycoside phosphotransferase Human genes 0.000 description 2
- 210000003719 b-lymphocyte Anatomy 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 108010047153 bovine corneal protein 54 Proteins 0.000 description 2
- 229960002092 busulfan Drugs 0.000 description 2
- 238000010804 cDNA synthesis Methods 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 230000032823 cell division Effects 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 108700017203 chicken ERNI Proteins 0.000 description 2
- 230000008711 chromosomal rearrangement Effects 0.000 description 2
- 239000012228 culture supernatant Substances 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 230000002939 deleterious effect Effects 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 210000002969 egg yolk Anatomy 0.000 description 2
- 230000013020 embryo development Effects 0.000 description 2
- 108700005856 engrailed 2 Proteins 0.000 description 2
- 230000007717 exclusion Effects 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 238000000684 flow cytometry Methods 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 102000018146 globin Human genes 0.000 description 2
- 108060003196 globin Proteins 0.000 description 2
- 235000015220 hamburgers Nutrition 0.000 description 2
- -1 his-D Natural products 0.000 description 2
- 238000010562 histological examination Methods 0.000 description 2
- 210000003016 hypothalamus Anatomy 0.000 description 2
- 230000000415 inactivating effect Effects 0.000 description 2
- 208000000509 infertility Diseases 0.000 description 2
- 230000036512 infertility Effects 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 230000009027 insemination Effects 0.000 description 2
- 229940125396 insulin Drugs 0.000 description 2
- 101150066555 lacZ gene Proteins 0.000 description 2
- 238000001638 lipofection Methods 0.000 description 2
- 238000012423 maintenance Methods 0.000 description 2
- 210000000953 male primordial germ cell Anatomy 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 238000010172 mouse model Methods 0.000 description 2
- 108020004707 nucleic acids Proteins 0.000 description 2
- 102000039446 nucleic acids Human genes 0.000 description 2
- 150000007523 nucleic acids Chemical class 0.000 description 2
- 210000004940 nucleus Anatomy 0.000 description 2
- 210000001672 ovary Anatomy 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 239000000825 pharmaceutical preparation Substances 0.000 description 2
- 229940127557 pharmaceutical product Drugs 0.000 description 2
- 230000001817 pituitary effect Effects 0.000 description 2
- 230000008488 polyadenylation Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 230000001850 reproductive effect Effects 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 230000003248 secreting effect Effects 0.000 description 2
- 238000005204 segregation Methods 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 230000000920 spermatogeneic effect Effects 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 238000010361 transduction Methods 0.000 description 2
- 230000026683 transduction Effects 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 238000003146 transient transfection Methods 0.000 description 2
- 208000001072 type 2 diabetes mellitus Diseases 0.000 description 2
- 230000002792 vascular Effects 0.000 description 2
- 238000001262 western blot Methods 0.000 description 2
- LAQPKDLYOBZWBT-NYLDSJSYSA-N (2s,4s,5r,6r)-5-acetamido-2-{[(2s,3r,4s,5s,6r)-2-{[(2r,3r,4r,5r)-5-acetamido-1,2-dihydroxy-6-oxo-4-{[(2s,3s,4r,5s,6s)-3,4,5-trihydroxy-6-methyloxan-2-yl]oxy}hexan-3-yl]oxy}-3,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy}-4-hydroxy-6-[(1r,2r)-1,2,3-trihydrox Chemical compound O[C@H]1[C@H](O)[C@H](O)[C@H](C)O[C@H]1O[C@H]([C@@H](NC(C)=O)C=O)[C@@H]([C@H](O)CO)O[C@H]1[C@H](O)[C@@H](O[C@]2(O[C@H]([C@H](NC(C)=O)[C@@H](O)C2)[C@H](O)[C@H](O)CO)C(O)=O)[C@@H](O)[C@@H](CO)O1 LAQPKDLYOBZWBT-NYLDSJSYSA-N 0.000 description 1
- RINPOXNCRXGFAV-FNXAHOOKSA-N (2s,4s,5r,6r)-5-amino-2-[(2s,3r,4r,5s,6r)-5-[(2s,3r,4r,5r,6r)-3-amino-5-hydroxy-6-(hydroxymethyl)-4-[(2r,3r,4s,5r,6r)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxan-2-yl]oxy-2-[(2r,3s,4r,5r,6r)-6-[(e)-2-amino-3-hydroxyicos-4-enoxy]-4,5-dihydroxy-2-(h Chemical compound O[C@@H]1[C@@H](O)[C@H](OCC(N)C(O)/C=C/CCCCCCCCCCCCCCC)O[C@H](CO)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@]2(O[C@H]([C@H](N)[C@@H](O)C2)C(O)C(O)CO)C(O)=O)[C@@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@@H](O)[C@@H](CO)O3)O)[C@@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RINPOXNCRXGFAV-FNXAHOOKSA-N 0.000 description 1
- 108091064702 1 family Proteins 0.000 description 1
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 1
- 101150066375 35 gene Proteins 0.000 description 1
- 108020003589 5' Untranslated Regions Proteins 0.000 description 1
- 101710159080 Aconitate hydratase A Proteins 0.000 description 1
- 101710159078 Aconitate hydratase B Proteins 0.000 description 1
- 206010000830 Acute leukaemia Diseases 0.000 description 1
- 102100031460 Advillin Human genes 0.000 description 1
- 235000003930 Aegle marmelos Nutrition 0.000 description 1
- 244000058084 Aegle marmelos Species 0.000 description 1
- 102100026605 Aldehyde dehydrogenase, dimeric NADP-preferring Human genes 0.000 description 1
- 108010083359 Antigen Receptors Proteins 0.000 description 1
- 241000269350 Anura Species 0.000 description 1
- 210000002237 B-cell of pancreatic islet Anatomy 0.000 description 1
- 241001260012 Bursa Species 0.000 description 1
- YDNKGFDKKRUKPY-JHOUSYSJSA-N C16 ceramide Natural products CCCCCCCCCCCCCCCC(=O)N[C@@H](CO)[C@H](O)C=CCCCCCCCCCCCCC YDNKGFDKKRUKPY-JHOUSYSJSA-N 0.000 description 1
- 241000283707 Capra Species 0.000 description 1
- 206010068051 Chimerism Diseases 0.000 description 1
- 108020004638 Circular DNA Proteins 0.000 description 1
- 238000002883 ClustalW sequence alignment Methods 0.000 description 1
- 102100022641 Coagulation factor IX Human genes 0.000 description 1
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- 108010001132 DNA Polymerase beta Proteins 0.000 description 1
- 238000012270 DNA recombination Methods 0.000 description 1
- 230000033616 DNA repair Effects 0.000 description 1
- 102100033672 Deleted in azoospermia-like Human genes 0.000 description 1
- 101000756636 Dictyostelium discoideum Major actin Proteins 0.000 description 1
- 102000016607 Diphtheria Toxin Human genes 0.000 description 1
- 108010053187 Diphtheria Toxin Proteins 0.000 description 1
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 description 1
- 108700028674 Drosophila vas Proteins 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- YQYJSBFKSSDGFO-UHFFFAOYSA-N Epihygromycin Natural products OC1C(O)C(C(=O)C)OC1OC(C(=C1)O)=CC=C1C=C(C)C(=O)NC1C(O)C(O)C2OCOC2C1O YQYJSBFKSSDGFO-UHFFFAOYSA-N 0.000 description 1
- 108700039887 Essential Genes Proteins 0.000 description 1
- 108700024394 Exon Proteins 0.000 description 1
- 101150092822 FGF5 gene Proteins 0.000 description 1
- 108010076282 Factor IX Proteins 0.000 description 1
- 102000018233 Fibroblast Growth Factor Human genes 0.000 description 1
- 108050007372 Fibroblast Growth Factor Proteins 0.000 description 1
- PNNNRSAQSRJVSB-SLPGGIOYSA-N Fucose Natural products C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C=O PNNNRSAQSRJVSB-SLPGGIOYSA-N 0.000 description 1
- 101150066002 GFP gene Proteins 0.000 description 1
- 101000834253 Gallus gallus Actin, cytoplasmic 1 Proteins 0.000 description 1
- 230000010558 Gene Alterations Effects 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229920002527 Glycogen Polymers 0.000 description 1
- 102000007390 Glycogen Phosphorylase Human genes 0.000 description 1
- 108010046163 Glycogen Phosphorylase Proteins 0.000 description 1
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 1
- 208000028782 Hereditary disease Diseases 0.000 description 1
- 108010034791 Heterochromatin Proteins 0.000 description 1
- 229920000209 Hexadimethrine bromide Polymers 0.000 description 1
- 101000729999 Homo sapiens Advillin Proteins 0.000 description 1
- 101000871280 Homo sapiens Deleted in azoospermia-like Proteins 0.000 description 1
- 101001127531 Homo sapiens Gastricsin Proteins 0.000 description 1
- 101000628899 Homo sapiens Small ubiquitin-related modifier 1 Proteins 0.000 description 1
- 101000784552 Homo sapiens Zinc finger X-linked protein ZXDA Proteins 0.000 description 1
- 101000784551 Homo sapiens Zinc finger X-linked protein ZXDB Proteins 0.000 description 1
- 101000784571 Homo sapiens Zinc finger protein ZXDC Proteins 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 102000003746 Insulin Receptor Human genes 0.000 description 1
- 102100039131 Integrator complex subunit 5 Human genes 0.000 description 1
- 101710092888 Integrator complex subunit 5 Proteins 0.000 description 1
- 108091029795 Intergenic region Proteins 0.000 description 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 description 1
- SHZGCJCMOBCMKK-DHVFOXMCSA-N L-fucopyranose Chemical compound C[C@@H]1OC(O)[C@@H](O)[C@H](O)[C@@H]1O SHZGCJCMOBCMKK-DHVFOXMCSA-N 0.000 description 1
- 241001599018 Melanogaster Species 0.000 description 1
- 208000024556 Mendelian disease Diseases 0.000 description 1
- 241000699660 Mus musculus Species 0.000 description 1
- 101100407657 Mus musculus Pgc gene Proteins 0.000 description 1
- 101100152729 Mus musculus Tenm4 gene Proteins 0.000 description 1
- SQVRNKJHWKZAKO-PFQGKNLYSA-N N-acetyl-beta-neuraminic acid Chemical compound CC(=O)N[C@@H]1[C@@H](O)C[C@@](O)(C(O)=O)O[C@H]1[C@H](O)[C@H](O)CO SQVRNKJHWKZAKO-PFQGKNLYSA-N 0.000 description 1
- CRJGESKKUOMBCT-VQTJNVASSA-N N-acetylsphinganine Chemical compound CCCCCCCCCCCCCCC[C@@H](O)[C@H](CO)NC(C)=O CRJGESKKUOMBCT-VQTJNVASSA-N 0.000 description 1
- HPFXACZRFJDURI-KTKRTIGZSA-N N-oleoylglycine Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)NCC(O)=O HPFXACZRFJDURI-KTKRTIGZSA-N 0.000 description 1
- BAWFJGJZGIEFAR-NNYOXOHSSA-O NAD(+) Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-O 0.000 description 1
- 241001045988 Neogene Species 0.000 description 1
- 101100329389 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) cre-1 gene Proteins 0.000 description 1
- 108010077850 Nuclear Localization Signals Proteins 0.000 description 1
- 102000007399 Nuclear hormone receptor Human genes 0.000 description 1
- 108020005497 Nuclear hormone receptor Proteins 0.000 description 1
- 102000002491 Octamer Transcription Factor-1 Human genes 0.000 description 1
- 108010068098 Octamer Transcription Factor-1 Proteins 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 238000010222 PCR analysis Methods 0.000 description 1
- 229930040373 Paraformaldehyde Natural products 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 101150009401 Prkcz gene Proteins 0.000 description 1
- 101710136296 Probable ATP-dependent RNA helicase DDX4 Proteins 0.000 description 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100021538 Protein kinase C zeta type Human genes 0.000 description 1
- LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 description 1
- 108091034057 RNA (poly(A)) Proteins 0.000 description 1
- 230000004570 RNA-binding Effects 0.000 description 1
- 101710105008 RNA-binding protein Proteins 0.000 description 1
- 102000018120 Recombinases Human genes 0.000 description 1
- 108010091086 Recombinases Proteins 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 102000037054 SLC-Transporter Human genes 0.000 description 1
- 108091006207 SLC-Transporter Proteins 0.000 description 1
- 101710081623 Small ubiquitin-related modifier 1 Proteins 0.000 description 1
- 108091081024 Start codon Proteins 0.000 description 1
- 241000282887 Suidae Species 0.000 description 1
- 108700025695 Suppressor Genes Proteins 0.000 description 1
- 101710143177 Synaptonemal complex protein 1 Proteins 0.000 description 1
- 102100036234 Synaptonemal complex protein 1 Human genes 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- HSCJRCZFDFQWRP-JZMIEXBBSA-N UDP-alpha-D-glucose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OP(O)(=O)OP(O)(=O)OC[C@@H]1[C@@H](O)[C@@H](O)[C@H](N2C(NC(=O)C=C2)=O)O1 HSCJRCZFDFQWRP-JZMIEXBBSA-N 0.000 description 1
- 108090000848 Ubiquitin Proteins 0.000 description 1
- 102000044159 Ubiquitin Human genes 0.000 description 1
- HSCJRCZFDFQWRP-UHFFFAOYSA-N Uridindiphosphoglukose Natural products OC1C(O)C(O)C(CO)OC1OP(O)(=O)OP(O)(=O)OCC1C(O)C(O)C(N2C(NC(=O)C=C2)=O)O1 HSCJRCZFDFQWRP-UHFFFAOYSA-N 0.000 description 1
- 108010067390 Viral Proteins Proteins 0.000 description 1
- 241000269368 Xenopus laevis Species 0.000 description 1
- 210000002593 Y chromosome Anatomy 0.000 description 1
- 108010084455 Zeocin Proteins 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 102100020909 Zinc finger X-linked protein ZXDA Human genes 0.000 description 1
- 102100020911 Zinc finger X-linked protein ZXDB Human genes 0.000 description 1
- 102100020906 Zinc finger protein ZXDC Human genes 0.000 description 1
- 230000003187 abdominal effect Effects 0.000 description 1
- 238000002679 ablation Methods 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- WQZGKKKJIJFFOK-PHYPRBDBSA-N alpha-D-galactose Chemical compound OC[C@H]1O[C@H](O)[C@H](O)[C@@H](O)[C@H]1O WQZGKKKJIJFFOK-PHYPRBDBSA-N 0.000 description 1
- 229960000723 ampicillin Drugs 0.000 description 1
- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000000692 anti-sense effect Effects 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- 210000000709 aorta Anatomy 0.000 description 1
- SQVRNKJHWKZAKO-UHFFFAOYSA-N beta-N-Acetyl-D-neuraminic acid Natural products CC(=O)NC1C(O)CC(O)(C(O)=O)OC1C(O)C(O)CO SQVRNKJHWKZAKO-UHFFFAOYSA-N 0.000 description 1
- 230000027455 binding Effects 0.000 description 1
- 210000002459 blastocyst Anatomy 0.000 description 1
- 210000001172 blastoderm Anatomy 0.000 description 1
- 210000000601 blood cell Anatomy 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 244000309464 bull Species 0.000 description 1
- 230000022131 cell cycle Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 229940106189 ceramide Drugs 0.000 description 1
- ZVEQCJWYRWKARO-UHFFFAOYSA-N ceramide Natural products CCCCCCCCCCCCCCC(O)C(=O)NC(CO)C(O)C=CCCC=C(C)CCCCCCCCC ZVEQCJWYRWKARO-UHFFFAOYSA-N 0.000 description 1
- 238000007385 chemical modification Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000000546 chi-square test Methods 0.000 description 1
- 230000003081 coactivator Effects 0.000 description 1
- 230000005757 colony formation Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000001010 compromised effect Effects 0.000 description 1
- 230000001143 conditioned effect Effects 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000005138 cryopreservation Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 210000000805 cytoplasm Anatomy 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000034373 developmental growth involved in morphogenesis Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 210000001198 duodenum Anatomy 0.000 description 1
- 235000013345 egg yolk Nutrition 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 230000008011 embryonic death Effects 0.000 description 1
- 210000004212 embryonic organizer Anatomy 0.000 description 1
- 230000006718 epigenetic regulation Effects 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 239000003797 essential amino acid Substances 0.000 description 1
- 235000020776 essential amino acid Nutrition 0.000 description 1
- 229960004222 factor ix Drugs 0.000 description 1
- 230000001605 fetal effect Effects 0.000 description 1
- 231100000562 fetal loss Toxicity 0.000 description 1
- 229940126864 fibroblast growth factor Drugs 0.000 description 1
- 244000144992 flock Species 0.000 description 1
- 238000001943 fluorescence-activated cell sorting Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 229930182830 galactose Natural products 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 238000012252 genetic analysis Methods 0.000 description 1
- 210000004392 genitalia Anatomy 0.000 description 1
- 238000010362 genome editing Methods 0.000 description 1
- 210000002980 germ line cell Anatomy 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 229940096919 glycogen Drugs 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 230000002710 gonadal effect Effects 0.000 description 1
- 230000009931 harmful effect Effects 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 210000002216 heart Anatomy 0.000 description 1
- 210000004458 heterochromatin Anatomy 0.000 description 1
- 102000050250 human PGC Human genes 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000002458 infectious effect Effects 0.000 description 1
- 231100000535 infertility Toxicity 0.000 description 1
- 238000009413 insulation Methods 0.000 description 1
- 230000003426 interchromosomal effect Effects 0.000 description 1
- 230000002427 irreversible effect Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 231100000225 lethality Toxicity 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 230000002934 lysing effect Effects 0.000 description 1
- 239000012139 lysis buffer Substances 0.000 description 1
- 210000003794 male germ cell Anatomy 0.000 description 1
- 210000003809 male germ line stem cell Anatomy 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 238000013507 mapping Methods 0.000 description 1
- 230000008774 maternal effect Effects 0.000 description 1
- 230000013011 mating Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 210000002161 motor neuron Anatomy 0.000 description 1
- 210000000663 muscle cell Anatomy 0.000 description 1
- 210000004165 myocardium Anatomy 0.000 description 1
- 101150091879 neo gene Proteins 0.000 description 1
- 230000009826 neoplastic cell growth Effects 0.000 description 1
- VVGIYYKRAMHVLU-UHFFFAOYSA-N newbouldiamide Natural products CCCCCCCCCCCCCCCCCCCC(O)C(O)C(O)C(CO)NC(=O)CCCCCCCCCCCCCCCCC VVGIYYKRAMHVLU-UHFFFAOYSA-N 0.000 description 1
- 102000045246 noggin Human genes 0.000 description 1
- 108700007229 noggin Proteins 0.000 description 1
- 108020004017 nuclear receptors Proteins 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 239000002777 nucleoside Substances 0.000 description 1
- 125000003835 nucleoside group Chemical group 0.000 description 1
- 230000030648 nucleus localization Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 238000011275 oncology therapy Methods 0.000 description 1
- 210000002380 oogonia Anatomy 0.000 description 1
- 230000002018 overexpression Effects 0.000 description 1
- 210000000496 pancreas Anatomy 0.000 description 1
- 229920002866 paraformaldehyde Polymers 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- CWCMIVBLVUHDHK-ZSNHEYEWSA-N phleomycin D1 Chemical compound N([C@H](C(=O)N[C@H](C)[C@@H](O)[C@H](C)C(=O)N[C@@H]([C@H](O)C)C(=O)NCCC=1SC[C@@H](N=1)C=1SC=C(N=1)C(=O)NCCCCNC(N)=N)[C@@H](O[C@H]1[C@H]([C@@H](O)[C@H](O)[C@H](CO)O1)O[C@@H]1[C@H]([C@@H](OC(N)=O)[C@H](O)[C@@H](CO)O1)O)C=1N=CNC=1)C(=O)C1=NC([C@H](CC(N)=O)NC[C@H](N)C(N)=O)=NC(N)=C1C CWCMIVBLVUHDHK-ZSNHEYEWSA-N 0.000 description 1
- 230000019612 pigmentation Effects 0.000 description 1
- 239000013600 plasmid vector Substances 0.000 description 1
- 108091033319 polynucleotide Proteins 0.000 description 1
- 102000040430 polynucleotide Human genes 0.000 description 1
- 239000002157 polynucleotide Substances 0.000 description 1
- 230000000270 postfertilization Effects 0.000 description 1
- 210000001811 primitive streak Anatomy 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000002818 protein evolution Methods 0.000 description 1
- 238000000751 protein extraction Methods 0.000 description 1
- 108010050991 protein kinase C zeta Proteins 0.000 description 1
- 108091035233 repetitive DNA sequence Proteins 0.000 description 1
- 102000053632 repetitive DNA sequence Human genes 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 230000000405 serological effect Effects 0.000 description 1
- 239000013605 shuttle vector Substances 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 230000001743 silencing effect Effects 0.000 description 1
- 238000002741 site-directed mutagenesis Methods 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 210000001082 somatic cell Anatomy 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 230000002463 transducing effect Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 238000011830 transgenic mouse model Methods 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- 238000001665 trituration Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
- A01K67/0276—Knock-out vertebrates
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
- C07K14/465—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from birds
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
- A01K2217/052—Animals comprising random inserted nucleic acids (transgenic) inducing gain of function
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/07—Animals genetically altered by homologous recombination
- A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2227/00—Animals characterised by species
- A01K2227/30—Bird
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/01—Animal expressing industrially exogenous proteins
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/03—Animal model, e.g. for test or diseases
- A01K2267/0393—Animal model comprising a reporter system for screening tests
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Environmental Sciences (AREA)
- Veterinary Medicine (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Immunology (AREA)
- Animal Behavior & Ethology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Mycology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US96489107P | 2007-08-14 | 2007-08-14 | |
| US60/964,891 | 2007-08-14 | ||
| PCT/US2008/073214 WO2009023800A1 (en) | 2007-08-14 | 2008-08-14 | Transgenic chickens with an inactivated endogenous gene locus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2008286791A1 true AU2008286791A1 (en) | 2009-02-19 |
Family
ID=40351167
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2008286791A Abandoned AU2008286791A1 (en) | 2007-08-14 | 2008-08-14 | Transgenic chickens with an inactivated endogenous gene locus |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP2187732A4 (ko) |
| JP (3) | JP5760159B2 (ko) |
| KR (1) | KR101563017B1 (ko) |
| CN (1) | CN101868144A (ko) |
| AU (1) | AU2008286791A1 (ko) |
| CA (1) | CA2706224A1 (ko) |
| WO (1) | WO2009023800A1 (ko) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9380769B2 (en) | 2011-05-24 | 2016-07-05 | Crystal Bioscience Inc. | Transgenic chicken comprising an inactivated immunoglobulin gene |
| AU2013204327B2 (en) * | 2012-04-20 | 2016-09-01 | Aviagen | Cell transfection method |
| KR20180038554A (ko) * | 2015-08-27 | 2018-04-16 | 크리스탈 바이오사이언스 주식회사 | 공통 경쇄를 갖는 항체 생산을 위한 유전자도입 동물 |
| AU2018336161A1 (en) * | 2017-09-19 | 2020-03-26 | The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) | Genome-edited birds |
| CN110484549B (zh) * | 2018-04-20 | 2023-10-03 | 北京大学 | 基因组靶向修饰方法 |
| MX2021010611A (es) * | 2019-03-05 | 2021-11-12 | The State Of Israel Ministry Of Agriculture & Rural Development Agricultural Res Organization Aro Vo | Aves con genoma editado. |
| WO2023127903A1 (ja) * | 2021-12-28 | 2023-07-06 | 国立大学法人北海道大学 | 始原生殖細胞が富化された胚を調製する方法 |
| CN115120382A (zh) * | 2022-05-26 | 2022-09-30 | 广东省农业科学院动物科学研究所 | 一种鸡生殖前体细胞移植受体的制备方法 |
| CN115191404B (zh) * | 2022-07-28 | 2023-04-21 | 四川省畜牧科学研究院 | 提高黄羽肉鸡后代公鸡比例的新品种选育方法 |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2357495T3 (es) * | 1997-10-16 | 2011-04-27 | University Of Georgia Research Foundation, Inc. | Aves transgénicas y producción de proteína. |
| GB2398784B (en) * | 2003-02-26 | 2005-07-27 | Babraham Inst | Removal and modification of the immunoglobulin constant region gene cluster of a non-human mammal |
| MXPA06000562A (es) * | 2003-07-15 | 2006-03-30 | Therapeutic Human Polyclonals | Loci de inmunoglobulina humanizada. |
| EP1850659B1 (en) * | 2005-02-01 | 2016-11-23 | Synageva BioPharma Corp. | Long term culture of chicken primordial germ cells |
-
2008
- 2008-08-14 WO PCT/US2008/073214 patent/WO2009023800A1/en active Application Filing
- 2008-08-14 EP EP08797925A patent/EP2187732A4/en not_active Withdrawn
- 2008-08-14 CA CA2706224A patent/CA2706224A1/en not_active Abandoned
- 2008-08-14 CN CN200880111586A patent/CN101868144A/zh active Pending
- 2008-08-14 AU AU2008286791A patent/AU2008286791A1/en not_active Abandoned
- 2008-08-14 JP JP2010521188A patent/JP5760159B2/ja not_active Expired - Fee Related
- 2008-08-14 KR KR1020107005724A patent/KR101563017B1/ko not_active IP Right Cessation
-
2014
- 2014-09-29 JP JP2014197988A patent/JP6004290B2/ja not_active Expired - Fee Related
-
2016
- 2016-04-14 JP JP2016080837A patent/JP2016129519A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2014239702A (ja) | 2014-12-25 |
| JP2016129519A (ja) | 2016-07-21 |
| EP2187732A1 (en) | 2010-05-26 |
| CN101868144A (zh) | 2010-10-20 |
| CA2706224A1 (en) | 2009-02-19 |
| JP6004290B2 (ja) | 2016-10-05 |
| JP5760159B2 (ja) | 2015-08-05 |
| KR101563017B1 (ko) | 2015-10-23 |
| KR20100088125A (ko) | 2010-08-06 |
| EP2187732A4 (en) | 2012-11-07 |
| JP2010536346A (ja) | 2010-12-02 |
| WO2009023800A1 (en) | 2009-02-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP6004290B2 (ja) | 不活化された内因性遺伝子座を有するトランスジェニックニワトリ | |
| US20170223938A1 (en) | Transgenic chickens with an inactivated endogenous gene locus | |
| ES2615081T3 (es) | Cultivo a largo plazo de células germinales primordiales de pollo | |
| US10689433B2 (en) | Transgenic chicken comprising an inactivated immunoglobulin gene | |
| US20120156771A1 (en) | Functional disruption of an immunoglobulin gene in an avian embryonic stem cell | |
| JP6644276B2 (ja) | 家禽始原生殖細胞の遺伝子改変方法、遺伝子改変された家禽始原生殖細胞、遺伝子改変家禽の生産方法及び家禽卵 | |
| US20220136003A1 (en) | Genome-edited birds | |
| US20240052304A1 (en) | Sterile avian embryos, production and uses thereof | |
| US20200214273A1 (en) | Genome-edited birds | |
| KR20210106412A (ko) | 유전적으로 변형된 불임 조류 및 이의 재구성 방법 | |
| EA043573B1 (ru) | Птицы с отредактированным геномом | |
| Johnson | Strategies to study sex-determining genes in the mouse |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PC1 | Assignment before grant (sect. 113) |
Owner name: SYMPHOGEN A/S Free format text: FORMER APPLICANT(S): ORIGEN THERAPEUTICS, INC. |
|
| MK1 | Application lapsed section 142(2)(a) - no request for examination in relevant period | ||
| TH | Corrigenda |
Free format text: IN VOL 26 , NO 40 , PAGE(S) 5213 UNDER THE HEADING APPLICATIONS LAPSED, REFUSED OR WITHDRAWN, PATENTS CEASED OR EXPIRED - 2008 DELETE ALL REFERENCE TO 2008286791. |
|
| MK1 | Application lapsed section 142(2)(a) - no request for examination in relevant period |