CA2664456C - Differenciation d'impulsions en cytometrie de flux, et applications - Google Patents

Differenciation d'impulsions en cytometrie de flux, et applications Download PDF

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Publication number
CA2664456C
CA2664456C CA2664456A CA2664456A CA2664456C CA 2664456 C CA2664456 C CA 2664456C CA 2664456 A CA2664456 A CA 2664456A CA 2664456 A CA2664456 A CA 2664456A CA 2664456 C CA2664456 C CA 2664456C
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Canada
Prior art keywords
particles
pulse
derivative
time
particle
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CA2664456A
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CA2664456A1 (fr
Inventor
Richard Pittaro
Bruce Goldman
Ray Lefebvre
David A. King
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EMD Millipore Corp
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EMD Millipore Corp
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Publication of CA2664456A1 publication Critical patent/CA2664456A1/fr
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Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N15/1429—Electro-optical investigation, e.g. flow cytometers using an analyser being characterised by its signal processing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1477—Multiparameters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Electro-optical investigation, e.g. flow cytometers
    • G01N2015/1486—Counting the particles

Abstract

L'invention concerne un procédé d'analyse d'impulsions à partir d'un cytomètre de flux dans lequel des particules présentes dans un fluide passent à travers un volume d'excitation d'un rayonnement électromagnétique et interagissent avec ledit rayonnement électromagnétique pour générer des signaux sous forme d'impulsions. Le procédé consiste à: générer une impulsion dépendante du temps indiquant les caractéristiques d'une ou de plusieurs particules passant à travers le volume d'excitation du rayonnement électromagnétique; déterminer une fenêtre de mesure par sélection d'une partie de l'impulsion présentant un point de départ et un point d'arrivée dont la valeur est supérieure à une valeur prédéterminée; et calculer un premier dérivé de l'impulsion par rapport au temps sur la fenêtre de mesure.
CA2664456A 2006-09-29 2007-09-27 Differenciation d'impulsions en cytometrie de flux, et applications Active CA2664456C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US84820006P 2006-09-29 2006-09-29
US60/848,200 2006-09-29
PCT/US2007/079761 WO2008042718A2 (fr) 2006-09-29 2007-09-27 Différenciation d'impulsions en cytométrie de flux, et applications

Publications (2)

Publication Number Publication Date
CA2664456A1 CA2664456A1 (fr) 2008-04-10
CA2664456C true CA2664456C (fr) 2013-12-31

Family

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Family Applications (1)

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CA2664456A Active CA2664456C (fr) 2006-09-29 2007-09-27 Differenciation d'impulsions en cytometrie de flux, et applications

Country Status (6)

Country Link
US (3) US7847923B2 (fr)
EP (1) EP2062056B1 (fr)
JP (1) JP4971451B2 (fr)
CN (1) CN101611323B (fr)
CA (1) CA2664456C (fr)
WO (1) WO2008042718A2 (fr)

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Also Published As

Publication number Publication date
US20110063602A1 (en) 2011-03-17
JP4971451B2 (ja) 2012-07-11
US7847923B2 (en) 2010-12-07
JP2010505132A (ja) 2010-02-18
CN101611323B (zh) 2013-02-20
CA2664456A1 (fr) 2008-04-10
US8184271B2 (en) 2012-05-22
WO2008042718A3 (fr) 2008-12-11
US20080221812A1 (en) 2008-09-11
US20120236291A1 (en) 2012-09-20
WO2008042718A2 (fr) 2008-04-10
US8885153B2 (en) 2014-11-11
EP2062056A4 (fr) 2018-01-10
EP2062056A2 (fr) 2009-05-27
CN101611323A (zh) 2009-12-23
EP2062056B1 (fr) 2019-04-10

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