CA2645136C - Oligonucleotides comprising signalling pairs and hydrophobic nucleotides, stemless beacons, for detection of nucleic acids, methylation status and mutants of nucleic acids - Google Patents

Oligonucleotides comprising signalling pairs and hydrophobic nucleotides, stemless beacons, for detection of nucleic acids, methylation status and mutants of nucleic acids Download PDF

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Publication number
CA2645136C
CA2645136C CA2645136A CA2645136A CA2645136C CA 2645136 C CA2645136 C CA 2645136C CA 2645136 A CA2645136 A CA 2645136A CA 2645136 A CA2645136 A CA 2645136A CA 2645136 C CA2645136 C CA 2645136C
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oligonucleotide analogue
oligonucleotide
hydrophobic
analogue
nucleotide
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CA2645136A
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French (fr)
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CA2645136A1 (en
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Ulf Bech Christensen
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA2645136A 2006-03-16 2007-03-16 Oligonucleotides comprising signalling pairs and hydrophobic nucleotides, stemless beacons, for detection of nucleic acids, methylation status and mutants of nucleic acids Active CA2645136C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA200600379 2006-03-16
DKPA200600379 2006-03-16
PCT/DK2007/000134 WO2007104318A2 (en) 2006-03-16 2007-03-16 Oligonucleotides comprising signalling pairs and hydrophobic nucleotides, stemless beacons, for detection of nucleic acids, methylation status and mutants of nucleic acids

Publications (2)

Publication Number Publication Date
CA2645136A1 CA2645136A1 (en) 2007-09-20
CA2645136C true CA2645136C (en) 2017-09-19

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CA2645136A Active CA2645136C (en) 2006-03-16 2007-03-16 Oligonucleotides comprising signalling pairs and hydrophobic nucleotides, stemless beacons, for detection of nucleic acids, methylation status and mutants of nucleic acids

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Country Link
US (1) US8133984B2 (enExample)
EP (1) EP2004847B1 (enExample)
JP (1) JP5284112B2 (enExample)
CN (1) CN101448957A (enExample)
AU (1) AU2007224843A1 (enExample)
CA (1) CA2645136C (enExample)
DK (1) DK2004847T3 (enExample)
NZ (1) NZ571966A (enExample)
WO (1) WO2007104318A2 (enExample)

Families Citing this family (15)

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Publication number Priority date Publication date Assignee Title
BRPI0820738A2 (pt) 2007-12-14 2015-06-16 Minitube America Inc Separação específica do gênero de células de esperma e embriões
US20110053226A1 (en) * 2008-06-13 2011-03-03 Riboxx Gmbh Method for enzymatic synthesis of chemically modified rna
DE102010012421B4 (de) 2009-03-23 2023-04-20 Vermicon Ag Verfahren zum spezifischen Nachweis von Mikroorganismen
WO2011032034A2 (en) 2009-09-10 2011-03-17 University Of Idaho Nucleobase-functionalized conformationally restricted nucleotides and oligonucleotides for targeting nucleic acids
JP5717169B2 (ja) * 2010-09-14 2015-05-13 国立大学法人名古屋大学 オリゴヌクレオチド及びその利用
AR088140A1 (es) 2011-07-19 2014-05-14 Univ Idaho Sonda y metodo para direccionar acidos nucleicos
EP2820157B1 (en) 2012-03-02 2019-05-01 Winthrop-University Hospital Method for using probe based pcr detection to measure the levels of circulating demethylated beta cell derived dna as a measure of beta cell loss in diabetes
CN102911111B (zh) * 2012-11-08 2014-06-11 齐鲁工业大学 一种咔唑苯甲醛缩对苯二胺双席夫碱及其制备方法
EP3350347B1 (en) 2015-09-14 2021-01-20 Pentabase APS Methods and materials for detection of mutations
WO2017218858A1 (en) 2016-06-15 2017-12-21 Genecapture, Inc. Compositions, methods and devices comprising stem-loop captor molecules
CN108827935B (zh) * 2018-06-08 2021-09-07 南京师范大学 一种基于金纳米孔阵列的dna甲基化表面增强拉曼散射光谱检测方法及其应用
CN112218877B (zh) 2018-08-27 2025-07-25 瑞泽恩制药公司 拉曼光谱在下游纯化中的应用
US20220205024A1 (en) * 2019-05-13 2022-06-30 Pentabase Aps Melting temperature methods, kits and reporter oligo for detecting variant nucleic acids
CN110261964B (zh) * 2019-07-01 2021-06-04 国检中心深圳珠宝检验实验室有限公司 一种用于光纤光谱仪的光纤头
JP2023540161A (ja) * 2020-06-29 2023-09-22 ペンタベース エーピーエス メチル化状態の検出

Family Cites Families (19)

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US5466578A (en) 1992-04-09 1995-11-14 The United States Of America As Represented By The Secretary Of The Navy Surfactant-enhanced light emission- or absorbance-based binding assays for polynucleic acids
US5925517A (en) 1993-11-12 1999-07-20 The Public Health Research Institute Of The City Of New York, Inc. Detectably labeled dual conformation oligonucleotide probes, assays and kits
US6312894B1 (en) 1995-04-03 2001-11-06 Epoch Pharmaceuticals, Inc. Hybridization and mismatch discrimination using oligonucleotides conjugated to minor groove binders
ATE196322T1 (de) 1995-05-05 2000-09-15 Perkin Elmer Corp Methoden and reagentien fuer die kombination einer pcr-amplifizierung mit einem hybridisierungs-assay
AU713667B2 (en) 1996-04-12 1999-12-09 Phri Properties, Inc. Detection probes, kits and assays
JPH11332595A (ja) 1997-07-09 1999-12-07 Masao Karube プローブｐｎａによるｄｎａの検出方法
DE69829760T3 (de) 1997-09-12 2016-04-14 Exiqon A/S Bi- und tri-zyklische - nukleosid, nukleotid und oligonukleotid-analoga
EP1025120B1 (en) 1997-10-27 2010-08-18 Boston Probes, Inc. Methods, kits and compositions pertaining to pna molecular beacons
US6485901B1 (en) 1997-10-27 2002-11-26 Boston Probes, Inc. Methods, kits and compositions pertaining to linear beacons
US6037130A (en) 1998-07-28 2000-03-14 The Public Health Institute Of The City Of New York, Inc. Wavelength-shifting probes and primers and their use in assays and kits
US20070059752A1 (en) * 1999-06-09 2007-03-15 Biosearch Technologies, Inc. Fluorescence energy transfer probes with stabilized conformations
DE60143974D1 (de) 2000-03-29 2011-03-10 Lgc Ltd Hybridisierungsprobe und Methode zum schnellen Nachweis und zur schnellen Unterscheidung von Sequenzen
WO2003043402A2 (en) * 2001-10-19 2003-05-30 Proligo Llc Nucleic acid probes and methods to detect and/or quantify nucleic acid analytes
CN1653079B (zh) 2001-12-18 2010-06-16 人类遗传标记控股有限公司 包括嵌入剂的假核苷酸
US20040086879A1 (en) 2001-12-20 2004-05-06 Yingufu Li Tripartite molecular beacons
WO2003072051A2 (en) 2002-02-27 2003-09-04 Biosource International Inc. Methods of using fet labeled oligonucleotides that include a 3' 5' exonuclease resistant quencher domain and compositions for practicing the same
GB0223563D0 (en) 2002-10-10 2002-11-20 Secr Defence Detection system
GB0319949D0 (en) 2003-08-26 2003-09-24 Univ Strathclyde Nucleic acid sequence identification
WO2005061734A2 (en) 2003-12-03 2005-07-07 Abbott Laboratories Double stranded linear nucleic acid probe and uses thereof

Also Published As

Publication number Publication date
WO2007104318A3 (en) 2008-03-20
CN101448957A (zh) 2009-06-03
NZ571966A (en) 2011-09-30
EP2004847A2 (en) 2008-12-24
JP5284112B2 (ja) 2013-09-11
JP2009529337A (ja) 2009-08-20
US20100068704A1 (en) 2010-03-18
WO2007104318A2 (en) 2007-09-20
US8133984B2 (en) 2012-03-13
CA2645136A1 (en) 2007-09-20
EP2004847B1 (en) 2017-05-03
DK2004847T3 (en) 2017-08-21
AU2007224843A1 (en) 2007-09-20

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