CA2635660C - Spider silk proteins and methods for producing spider silk proteins - Google Patents

Spider silk proteins and methods for producing spider silk proteins Download PDF

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Publication number
CA2635660C
CA2635660C CA2635660A CA2635660A CA2635660C CA 2635660 C CA2635660 C CA 2635660C CA 2635660 A CA2635660 A CA 2635660A CA 2635660 A CA2635660 A CA 2635660A CA 2635660 C CA2635660 C CA 2635660C
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CA
Canada
Prior art keywords
ala ala
gly
amino acid
ser
protein
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2635660A
Other languages
English (en)
French (fr)
Other versions
CA2635660A1 (en
Inventor
Jan Johansson
Goeran Hjaelm
Margareta Stark
Anna Rising
Stefan Grip
Wilhelm Engstroem
My Hedhammar
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Spiber Technologies AB
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Spiber Technologies AB
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Publication date
Application filed by Spiber Technologies AB filed Critical Spiber Technologies AB
Publication of CA2635660A1 publication Critical patent/CA2635660A1/en
Application granted granted Critical
Publication of CA2635660C publication Critical patent/CA2635660C/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61KPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/42Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
    • DTEXTILES; PAPER
    • D01NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01FCHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
    • D01F4/00Monocomponent artificial filaments or the like of proteins; Manufacture thereof

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Insects & Arthropods (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Epidemiology (AREA)
  • Toxicology (AREA)
  • Genetics & Genomics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Textile Engineering (AREA)
  • General Chemical & Material Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Inorganic Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CA2635660A 2005-12-30 2006-12-28 Spider silk proteins and methods for producing spider silk proteins Expired - Fee Related CA2635660C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE0502932 2005-12-30
SE0502932-7 2005-12-30
PCT/SE2006/001505 WO2007078239A2 (en) 2005-12-30 2006-12-28 Spider silk proteins and methods for producing spider silk proteins

Publications (2)

Publication Number Publication Date
CA2635660A1 CA2635660A1 (en) 2007-07-12
CA2635660C true CA2635660C (en) 2016-02-23

Family

ID=37836847

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2635660A Expired - Fee Related CA2635660C (en) 2005-12-30 2006-12-28 Spider silk proteins and methods for producing spider silk proteins

Country Status (10)

Country Link
US (4) US8173772B2 (enExample)
EP (2) EP2899204B1 (enExample)
JP (1) JP5192391B2 (enExample)
CN (1) CN101395178B (enExample)
AU (1) AU2006333611B2 (enExample)
CA (1) CA2635660C (enExample)
DK (1) DK1976868T3 (enExample)
ES (1) ES2533555T3 (enExample)
RU (1) RU2444527C2 (enExample)
WO (1) WO2007078239A2 (enExample)

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CA2815267C (en) * 2010-10-27 2019-06-25 Spiber Technologies Ab Spider silk fusion protein structures for binding to an organic target
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WO2012165476A1 (ja) * 2011-06-01 2012-12-06 スパイバー株式会社 人造ポリペプチド繊維及びその製造方法
WO2013065651A1 (ja) * 2011-11-02 2013-05-10 スパイバー株式会社 タンパク質溶液及びこれを用いたタンパク質繊維の製造方法
ES2653253T3 (es) 2011-11-02 2018-02-06 Spiber Inc. Solución de polipéptido, método de producción de fibra de polipéptido artificial que usa la misma, y método de purificación de polipéptido
JP6412866B2 (ja) 2012-05-02 2018-10-24 スパイバー テクノロジーズ アーベーSpiber Technologies Ab 有機ターゲットに結合するための反復断片を持たないクモ糸融合タンパク質の構造
WO2013164404A1 (en) 2012-05-02 2013-11-07 Spiber Technologies Ab Spider silk fusion protein structures incorporating immunoglobulin fragments as affinity ligands
WO2014002605A1 (ja) 2012-06-28 2014-01-03 スパイバー株式会社 原着タンパク質繊維及びその製造方法
WO2014052975A1 (en) * 2012-09-28 2014-04-03 Utah State University Expression systems and methods of producing spider silk proteins
US10899803B2 (en) 2012-10-17 2021-01-26 Nanyang Technological University Compounds and methods for the production of suckerin and uses thereof
EP3973992A3 (en) 2013-09-17 2022-08-31 Bolt Threads, Inc. Methods and compositions for synthesizing improved silk fibers
CN107849100B (zh) 2015-04-09 2022-02-08 丝芭博株式会社 极性溶剂溶液及其制造方法
WO2016163337A1 (ja) 2015-04-09 2016-10-13 Spiber株式会社 極性溶媒溶液及びその製造方法
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WO2017025964A1 (en) 2015-08-10 2017-02-16 Seevix Material Sciences Ltd. Compositions and methods for fabricating synthetic dragline spider silk
JP2018159137A (ja) 2015-08-20 2018-10-11 国立研究開発法人理化学研究所 クモ糸様構造を有するポリペプチド繊維の製造方法
WO2017138002A1 (en) 2016-02-11 2017-08-17 Seevix Material Sciences Ltd. Composite materials comprising synthetic dragline spider silk
EP3246051A1 (en) 2016-05-16 2017-11-22 Spiber Technologies AB Spider silk coating of solid surfaces
CA3024270A1 (en) 2016-05-16 2017-11-23 Spiber Technologies Ab Spider silk coating of solid surfaces
EP3263593A1 (en) 2016-07-01 2018-01-03 Anna Rising Engineered spider silk proteins and uses thereof
US11447532B2 (en) 2016-09-14 2022-09-20 Bolt Threads, Inc. Long uniform recombinant protein fibers
JP2020097796A (ja) * 2017-03-10 2020-06-25 Spiber株式会社 人造フィブロイン繊維
JP6337252B1 (ja) 2017-03-10 2018-06-06 Spiber株式会社 高収縮人造フィブロイン繊維及びその製造方法、並びに人造フィブロイン繊維の収縮方法
WO2018229341A1 (en) 2017-06-13 2018-12-20 Aalto University Foundation Sr. Method for producing a condensed adhesive phase of silk fusion proteins
US20200130335A1 (en) * 2017-07-04 2020-04-30 Spiber Inc. Composite Material and Composite Material Manufacturing Method
RU2020113065A (ru) 2017-09-26 2021-10-27 Спайбер Текнолоджиз Аб Структурирование поверхностно-активных макромолекул
JP7367977B2 (ja) * 2018-01-31 2023-10-24 Spiber株式会社 タンパク質捲縮ステープルの製造方法
CN108985298B (zh) * 2018-06-19 2022-02-18 浙江大学 一种基于语义一致性的人体衣物分割方法
CN113260745A (zh) * 2019-01-09 2021-08-13 丝芭博株式会社 改造丝心蛋白
CN109912720B (zh) * 2019-03-14 2021-12-07 天津大学 一种蛛丝蛋白的设计合成方法和纺丝
AU2021258592A1 (en) 2020-04-23 2022-10-06 Seevix Material Sciences Ltd. Modified spider silk fiber and use thereof
WO2022048733A1 (en) 2020-09-01 2022-03-10 Spiber Technologies Ab Method for producing macroporous scaffolds composed of silk and calcium phosphate
CN112080493B (zh) * 2020-09-07 2021-12-28 中国科学院天津工业生物技术研究所 制备串联重复dna的方法及相关生物材料与应用
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Also Published As

Publication number Publication date
RU2444527C2 (ru) 2012-03-10
WO2007078239A2 (en) 2007-07-12
WO2007078239A3 (en) 2007-12-21
US20140079674A1 (en) 2014-03-20
JP5192391B2 (ja) 2013-05-08
ES2533555T3 (es) 2015-04-13
US20090226969A1 (en) 2009-09-10
EP2899204A1 (en) 2015-07-29
EP2899204B1 (en) 2017-06-28
US8173772B2 (en) 2012-05-08
JP2009521921A (ja) 2009-06-11
CN101395178B (zh) 2013-05-01
EP1976868B1 (en) 2015-02-18
AU2006333611A1 (en) 2007-07-12
AU2006333611B2 (en) 2012-04-26
CA2635660A1 (en) 2007-07-12
US8278416B1 (en) 2012-10-02
US20120329992A1 (en) 2012-12-27
CN101395178A (zh) 2009-03-25
US8618255B2 (en) 2013-12-31
RU2008131298A (ru) 2010-02-10
DK1976868T3 (da) 2015-04-20
US8729235B2 (en) 2014-05-20
US20120264914A1 (en) 2012-10-18
EP1976868A2 (en) 2008-10-08

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Effective date: 20211229