CN101395178B - 蜘蛛丝蛋白和用于产生蜘蛛丝蛋白的方法 - Google Patents

蜘蛛丝蛋白和用于产生蜘蛛丝蛋白的方法 Download PDF

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CN101395178B
CN101395178B CN2006800535908A CN200680053590A CN101395178B CN 101395178 B CN101395178 B CN 101395178B CN 2006800535908 A CN2006800535908 A CN 2006800535908A CN 200680053590 A CN200680053590 A CN 200680053590A CN 101395178 B CN101395178 B CN 101395178B
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protein
amino acid
seq
spidroin
fusion protein
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CN101395178A (zh
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J·约翰松
G·耶尔姆
M·斯塔克
A·里辛
S·格里普
W·恩斯特伦
M·赫德哈马尔
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Spiber Technologies AB
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Spiber Technologies AB
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43513—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae
    • C07K14/43518—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from arachnidae from spiders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
    • A61K47/42—Proteins; Polypeptides; Degradation products thereof; Derivatives thereof, e.g. albumin, gelatin or zein
    • D—TEXTILES; PAPER
    • D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
    • D01F—CHEMICAL FEATURES IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS; APPARATUS SPECIALLY ADAPTED FOR THE MANUFACTURE OF CARBON FILAMENTS
    • D01F4/00—Monocomponent artificial filaments or the like of proteins; Manufacture thereof

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Insects & Arthropods (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Public Health (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Veterinary Medicine (AREA)
  • Biochemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Textile Engineering (AREA)
  • Inorganic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN2006800535908A 2005-12-30 2006-12-28 蜘蛛丝蛋白和用于产生蜘蛛丝蛋白的方法 Active CN101395178B (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
SE05029327 2005-12-30
SE0502932 2005-12-30
SE0502932-7 2005-12-30
PCT/SE2006/001505 WO2007078239A2 (en) 2005-12-30 2006-12-28 Spider silk proteins and methods for producing spider silk proteins

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CN101395178A CN101395178A (zh) 2009-03-25
CN101395178B true CN101395178B (zh) 2013-05-01

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US (4) US8173772B2 (enExample)
EP (2) EP2899204B1 (enExample)
JP (1) JP5192391B2 (enExample)
CN (1) CN101395178B (enExample)
AU (1) AU2006333611B2 (enExample)
CA (1) CA2635660C (enExample)
DK (1) DK1976868T3 (enExample)
ES (1) ES2533555T3 (enExample)
RU (1) RU2444527C2 (enExample)
WO (1) WO2007078239A2 (enExample)

Families Citing this family (47)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9051383B2 (en) * 2007-06-11 2015-06-09 The Regents Of The University Of California Spider silk dragline polynucleotides, polypeptides and methods of use thereof
EP2243792A1 (en) 2009-04-22 2010-10-27 Spiber Technologies AB Methods of producing polymers of spider silk proteins
WO2010123450A1 (en) 2009-04-22 2010-10-28 Spiber Technologies Ab Method of producing polymers of spider silk proteins
CN101724920B (zh) * 2009-11-13 2011-04-27 东华大学 干纺制备再生蚕丝纤维的方法
US9233067B2 (en) 2009-11-30 2016-01-12 Amsilk Gmbh Silk particles for controlled and sustained delivery of compounds
WO2011113446A1 (en) * 2010-03-17 2011-09-22 Amsilk Gmbh Method for production of polypeptide containing fibres
CA2792476C (en) 2010-03-18 2018-12-11 Spiber Technologies Ab Production of proteins and polypeptides
DK2558137T3 (en) 2010-04-12 2017-07-03 Spiber Tech Ab Methods and combination
DK3178843T3 (da) 2010-10-27 2019-08-19 Spiber Tech Ab Edderkoppesilke-fusionsproteinstrukturer til at binde til et organisk mål
RU2451023C1 (ru) * 2010-11-25 2012-05-20 Владимир Григорьевич Богуш Способ получения рекомбинантного белка паутины, слитый белок, рекомбинантная днк, вектор экспрессии, клетка-хозяин и штаммы-продуценты
CN103502516B (zh) * 2011-06-01 2015-04-22 丝芭博株式会社 人造多肽纤维及其制造方法
US9051453B2 (en) 2011-11-02 2015-06-09 Spiber Inc. Polypeptide solution, artificial polypeptide fiber production method and polypeptide purification method using same
WO2013065651A1 (ja) * 2011-11-02 2013-05-10 スパイバー株式会社 タンパク質溶液及びこれを用いたタンパク質繊維の製造方法
US9708376B2 (en) 2012-05-02 2017-07-18 Spiber Technologies Ab Spider silk fusion protein structures without repetitive fragment for binding to an organic target
CN104470607B (zh) * 2012-05-02 2017-04-05 思百博技术股份公司 作为亲和配体的掺入有免疫球蛋白片段的蜘蛛丝融合蛋白结构
EP2868782B1 (en) 2012-06-28 2020-07-15 Spiber Inc. Spun-dyed protein fiber and method for producing same
WO2014052975A1 (en) * 2012-09-28 2014-04-03 Utah State University Expression systems and methods of producing spider silk proteins
SG11201502932TA (en) 2012-10-17 2015-05-28 Univ Nanyang Tech Compounds and methods for the production of suckerin and uses thereof
EP3046585B1 (en) 2013-09-17 2021-08-25 Bolt Threads, Inc. Methods and compositions for synthesizing improved silk fibers
CN107735406A (zh) 2015-04-09 2018-02-23 丝芭博株式会社 极性溶剂溶液及其制造方法
US20180080147A1 (en) 2015-04-09 2018-03-22 Spiber Inc. Polar solvent solution and production method thereof
EP3307765B1 (en) * 2015-06-11 2024-04-10 Bolt Threads, Inc. Recombinant protein fiber yarns with improved properties
CN105031723B (zh) * 2015-06-23 2017-05-24 上海交通大学 基于蜘蛛丝蛋白的温敏性水凝胶
KR20180022873A (ko) 2015-06-26 2018-03-06 스파이버 테크놀러지스 에이비 고리형 rgd 세포-결합 모티프 및 이의 용도
AU2016305360B2 (en) 2015-08-10 2021-11-04 Seevix Material Sciences Ltd. Compositions and methods for fabricating synthetic dragline spider silk
JP2018159137A (ja) 2015-08-20 2018-10-11 国立研究開発法人理化学研究所 クモ糸様構造を有するポリペプチド繊維の製造方法
JP7176736B2 (ja) 2016-02-11 2022-11-22 シービックス マテリアル サイエンシーズ リミテッド 合成のクモドラグラインシルクを含む複合材料
CN109562200B (zh) 2016-05-16 2022-09-09 思百博技术股份公司 固体表面的蜘蛛丝涂层
EP3246051A1 (en) 2016-05-16 2017-11-22 Spiber Technologies AB Spider silk coating of solid surfaces
EP3263593A1 (en) 2016-07-01 2018-01-03 Anna Rising Engineered spider silk proteins and uses thereof
KR20190046993A (ko) 2016-09-14 2019-05-07 볼트 쓰레즈, 인크. 긴 균일한 재조합 단백질 섬유
JP6337252B1 (ja) * 2017-03-10 2018-06-06 Ｓｐｉｂｅｒ株式会社 高収縮人造フィブロイン繊維及びその製造方法、並びに人造フィブロイン繊維の収縮方法
JP2020097796A (ja) * 2017-03-10 2020-06-25 Ｓｐｉｂｅｒ株式会社 人造フィブロイン繊維
US11597750B2 (en) 2017-06-13 2023-03-07 Aalto University Foundation Sr Method for producing a condensed adhesive phase of silk fusion proteins
WO2019009302A1 (ja) * 2017-07-04 2019-01-10 内浜化成株式会社 複合材および複合材の製造方法
US12059826B2 (en) 2017-09-26 2024-08-13 Spiber Technologies Ab Structuring of surface-active macromolecules
EP3748066A4 (en) * 2018-01-31 2021-12-15 Spiber Inc. PROCESS FOR MAKING A STAPLE SET WITH PROTEIN
CN108985298B (zh) * 2018-06-19 2022-02-18 浙江大学 一种基于语义一致性的人体衣物分割方法
WO2020145363A1 (ja) 2019-01-09 2020-07-16 Ｓｐｉｂｅｒ株式会社 改変フィブロイン
CN109912720B (zh) * 2019-03-14 2021-12-07 天津大学 一种蛛丝蛋白的设计合成方法和纺丝
WO2021214780A1 (en) 2020-04-23 2021-10-28 Seevix Material Sciences Ltd. Modified spider silk fiber and use thereof
US20240033402A1 (en) 2020-09-01 2024-02-01 Spiber Technologies Ab Method for producing macroporous scaffolds composed of silk and calcium phosphate
CN112080493B (zh) * 2020-09-07 2021-12-28 中国科学院天津工业生物技术研究所 制备串联重复dna的方法及相关生物材料与应用
US20240141003A1 (en) * 2021-02-25 2024-05-02 Washington University Microbial production of polymeric amyloid fibers
WO2022218876A1 (en) 2021-04-12 2022-10-20 Biocrine Ab Pancreatic islet organoids as protein factories
US12391912B2 (en) 2021-10-26 2025-08-19 Rensselaer Polytechnic Institute Systems and methods for increased production of recombinant biopolymers via genome engineering and downregulation of basal expression
CN114907467B (zh) * 2022-05-13 2023-09-15 四川轻化工大学 一种融合碳端的重组蛛丝蛋白及其制备方法与基于重组蛛丝蛋白的载药微球

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE189699T1 (de) 1990-04-20 2000-02-15 Univ Wyoming Für spinnenseideprotein kodierende dns, dns enthaltender vektor, transformierte zelle und produkte davon
TW336938B (en) * 1992-05-21 1998-07-21 Pfizer Calcium channel blocking polypeptides from filistata hibernalis
WO1994029450A2 (en) 1993-06-15 1994-12-22 E.I. Du Pont De Nemours And Company Novel, recombinantly produced spider silk analogs
US5733771A (en) 1994-03-14 1998-03-31 University Of Wyoming cDNAs encoding minor ampullate spider silk proteins
JPH11511325A (ja) 1995-08-22 1999-10-05 アグリコラ・テクノロジーズ・インコーポレイテツド 高力クモシルク蛋白質のクローニング方法
US5994099A (en) 1997-12-31 1999-11-30 The University Of Wyoming Extremely elastic spider silk protein and DNA coding therefor
US6620917B1 (en) * 2000-01-20 2003-09-16 The United States Of America As Represented By The Secretary Of The Army Method for the purification and aqueous fiber spinning of spider silks and other structural proteins
US6608242B1 (en) 2000-05-25 2003-08-19 E. I. Du Pont De Nemours And Company Production of silk-like proteins in plants
DE10113781A1 (de) 2000-06-09 2001-12-13 Ipk Inst Fuer Pflanzengenetik Synthetische Spinnenseidenproteine und deren Expression in transgenen Pflanzen
WO2002099082A2 (en) 2001-06-06 2002-12-12 University Of Wyoming Expression of spider silk proteins in higher plants
AU2002306944A1 (en) * 2001-08-29 2003-03-18 University Of Wyoming Spider silk protein encoding nucleic acids, polypeptides, antibodies and method of use thereof
AU2003202949A1 (en) 2002-01-11 2003-07-30 Ali Alwattari Methods and apparatus for spinning spider silk protein
GB2399820A (en) 2002-01-11 2004-09-29 Nexia Biotech Inc Recovery of biofilament proteins from biological fluids
WO2003057727A1 (en) 2002-01-11 2003-07-17 Nexia Biotechnologies, Inc. Methods of producing silk polypeptides and products thereof
GB0218977D0 (en) 2002-08-15 2002-09-25 Univ York Polypeptide
US20040132978A1 (en) 2002-11-12 2004-07-08 Fahnestock Stephen R. Method for purifying and recovering silk proteins in soluble form and uses thereof
US7060260B2 (en) 2003-02-20 2006-06-13 E.I. Du Pont De Nemours And Company Water-soluble silk proteins in compositions for skin care, hair care or hair coloring
FR2871822B1 (fr) 2004-06-17 2007-10-05 Icopal Sas Soc Par Actions Sim Isolant mince reflechissant pour sous-toiture
EP1609801A1 (en) * 2004-06-25 2005-12-28 Technische Universität München Proteins of natural origin and materials made therefrom
AU2005263622B2 (en) 2004-07-22 2012-04-26 Amsilk Gmbh Recombinant spider silk proteins
WO2006083514A2 (en) 2005-01-14 2006-08-10 The Florida International University Board Of Trustees Transgenic production of spider silk
EP1919938B1 (en) 2005-08-29 2012-06-06 AMSilk GmbH Modified spider silk proteins
EP1852470A1 (en) 2006-05-03 2007-11-07 Technische Universität München Multilayer Silk Protein Films

Non-Patent Citations (5)

* Cited by examiner, † Cited by third party
Title
Anna Rising et al..N-terminal nonrepetitive domain common to dragline, flagelliform, and cylindriform spider silk proteins.《Biomacromolecules》.2006,第7卷(第11期), *
LEWIS RANDOLPH V ET AL..Expression and Purification of a Spider Silk Protein A New Strategy for Producing Repetitive Proteins:A NEW strategy for producing repetitive proteins.《PROTEIN EXPRESSION AND PURIFICATION》.1996,第7卷(第4期),
LEWIS RANDOLPH V ET AL..Expression and Purification of a Spider Silk Protein A New Strategy for Producing Repetitive Proteins:A NEW strategy for producing repetitive proteins.《PROTEIN EXPRESSION AND PURIFICATION》.1996,第7卷(第4期), *
Natalia N. Pouchkina-Stantcheva et al..Molecular studies of a novel dragline silk from a nursery web spider, Euprosthenops sp..《Comparative biochemistry and physiology part b biochemistry & molecular biology》.2004,第138卷(第4期), *
SPONNER ET AL..The conserved C-termini contribute to the properties of spider silk fibroins.《Biochemical and biophysical research communications》.2005,第338卷(第2期), *

Also Published As

Publication number Publication date
ES2533555T3 (es) 2015-04-13
WO2007078239A2 (en) 2007-07-12
JP5192391B2 (ja) 2013-05-08
US8729235B2 (en) 2014-05-20
JP2009521921A (ja) 2009-06-11
US20090226969A1 (en) 2009-09-10
AU2006333611B2 (en) 2012-04-26
US20140079674A1 (en) 2014-03-20
WO2007078239A3 (en) 2007-12-21
AU2006333611A1 (en) 2007-07-12
CA2635660A1 (en) 2007-07-12
US20120264914A1 (en) 2012-10-18
US8278416B1 (en) 2012-10-02
RU2444527C2 (ru) 2012-03-10
EP2899204B1 (en) 2017-06-28
DK1976868T3 (da) 2015-04-20
EP1976868B1 (en) 2015-02-18
CN101395178A (zh) 2009-03-25
EP1976868A2 (en) 2008-10-08
RU2008131298A (ru) 2010-02-10
US20120329992A1 (en) 2012-12-27
US8618255B2 (en) 2013-12-31
US8173772B2 (en) 2012-05-08
EP2899204A1 (en) 2015-07-29
CA2635660C (en) 2016-02-23

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