CA2562390C - Novel methods for quantification of micrornas and small interfering rnas - Google Patents

Novel methods for quantification of micrornas and small interfering rnas Download PDF

Info

Publication number
CA2562390C
CA2562390C CA2562390A CA2562390A CA2562390C CA 2562390 C CA2562390 C CA 2562390C CA 2562390 A CA2562390 A CA 2562390A CA 2562390 A CA2562390 A CA 2562390A CA 2562390 C CA2562390 C CA 2562390C
Authority
CA
Canada
Prior art keywords
sequence
rna
probe
target
lna
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2562390A
Other languages
English (en)
French (fr)
Other versions
CA2562390A1 (en
Inventor
Nana Jacobsen
Lars Kongsbak
Sakari Kauppinen
Soren Morgenthaler Echwald
Peter Mouritzen
Peter Stein Nielsen
Mikkel Norholm
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Exiqon AS
Original Assignee
Exiqon AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Exiqon AS filed Critical Exiqon AS
Publication of CA2562390A1 publication Critical patent/CA2562390A1/en
Application granted granted Critical
Publication of CA2562390C publication Critical patent/CA2562390C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
CA2562390A 2004-04-07 2005-04-07 Novel methods for quantification of micrornas and small interfering rnas Expired - Lifetime CA2562390C (en)

Applications Claiming Priority (11)

Application Number Priority Date Filing Date Title
DKPA200400578 2004-04-07
DKPA200400578 2004-04-07
DKPA200401146 2004-07-23
DKPA200401146 2004-07-23
DKPA200401218 2004-08-11
DKPA200401218 2004-08-11
DKPA200401587 2004-10-15
DKPA200401587 2004-10-15
DKPA200500140 2005-01-28
DKPA200500140 2005-01-28
PCT/DK2005/000239 WO2005098029A2 (en) 2004-04-07 2005-04-07 Methods for quantification of micrornas and small interfering rnas

Publications (2)

Publication Number Publication Date
CA2562390A1 CA2562390A1 (en) 2005-10-20
CA2562390C true CA2562390C (en) 2014-12-02

Family

ID=35116021

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2562390A Expired - Lifetime CA2562390C (en) 2004-04-07 2005-04-07 Novel methods for quantification of micrornas and small interfering rnas

Country Status (8)

Country Link
EP (1) EP1735459B1 (enExample)
JP (1) JP5192229B2 (enExample)
CN (1) CN1961081B (enExample)
AT (1) ATE542918T1 (enExample)
CA (1) CA2562390C (enExample)
DK (1) DK1735459T3 (enExample)
ES (1) ES2380923T3 (enExample)
WO (1) WO2005098029A2 (enExample)

Families Citing this family (69)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040219565A1 (en) 2002-10-21 2004-11-04 Sakari Kauppinen Oligonucleotides useful for detecting and analyzing nucleic acids of interest
US8192937B2 (en) 2004-04-07 2012-06-05 Exiqon A/S Methods for quantification of microRNAs and small interfering RNAs
US20070099196A1 (en) * 2004-12-29 2007-05-03 Sakari Kauppinen Novel oligonucleotide compositions and probe sequences useful for detection and analysis of micrornas and their target mRNAs
US8071306B2 (en) 2005-01-25 2011-12-06 Merck Sharp & Dohme Corp. Methods for quantitating small RNA molecules
JP2008528003A (ja) * 2005-01-25 2008-07-31 ロゼッタ インファーマティックス エルエルシー 小ｒｎａ分子の定量方法
WO2006130761A1 (en) * 2005-05-31 2006-12-07 Applera Corporation Linear amplification of short nucleic acids
JP2007082436A (ja) * 2005-09-20 2007-04-05 Bioinformatics Institute For Global Good Inc 機能性ＲＮＡが制御するターゲットｍＲＮＡの予測・同定方法及びその利用方法
US20100286044A1 (en) * 2005-12-29 2010-11-11 Exiqon A/S Detection of tissue origin of cancer
EA201100811A1 (ru) 2006-04-03 2012-06-29 Сантарис Фарма А/С Фармацевтическая композиция
US8207325B2 (en) 2006-04-03 2012-06-26 Univ. of Copenhagen MicroRNA biomarkers for human breast and lung cancer
US7955848B2 (en) 2006-04-03 2011-06-07 Trustees Of Dartmouth College MicroRNA biomarkers for human breast and lung cancer
CA3042781C (en) 2006-04-03 2021-10-19 Roche Innovation Center Copenhagen A/S Pharmaceutical composition comprising anti-mirna antisense oligonucleotides
US8580494B2 (en) 2006-08-25 2013-11-12 Research Foundation For Mental Hygiene, Inc. Methods and compositions for amplification and detection of MicroRNAs
WO2008040355A2 (en) * 2006-10-06 2008-04-10 Exiqon A/S Novel methods for quantification of micrornas and small interfering rnas
EP2090665A2 (en) 2006-10-20 2009-08-19 Exiqon A/S Novel human microRNAs associated with cancer
US8188255B2 (en) 2006-10-20 2012-05-29 Exiqon A/S Human microRNAs associated with cancer
EP1914317A1 (en) * 2006-10-21 2008-04-23 Biolytix AG A method for qualitative and quantitative detection of short nucleic acid sequences of about 8 to 50 nucleotides in length
US7645578B2 (en) 2006-10-31 2010-01-12 Agilent Technologies, Inc. Cleavage of RNA at redundant sites
US8470791B2 (en) 2007-03-22 2013-06-25 Santaris Pharma A/S RNA antagonist compounds for the inhibition of Apo-B100 expression
US8580756B2 (en) 2007-03-22 2013-11-12 Santaris Pharma A/S Short oligomer antagonist compounds for the modulation of target mRNA
EP1978104A1 (en) * 2007-04-03 2008-10-08 Freie Universität Berlin A method for the quantitative analysis of RNA molecules in a sample
DK2205737T3 (da) 2007-10-04 2013-05-21 Santaris Pharma As Mikromirer
AU2008310955B2 (en) * 2007-10-09 2015-01-29 Igor Vassily Kutyavin Methods, compositions and kits for the improved detection of small RNA molecules
WO2009062169A2 (en) 2007-11-09 2009-05-14 The Board Of Regents Of The University Of Texas System Micro-rnas of the mir-15 family modulate cardiomyocyte survival and cardiac repair
US20110033858A1 (en) * 2008-02-04 2011-02-10 Genecare Research Institute Co., Ltd. siRNA DETECTION METHOD
EP2268811A1 (en) 2008-03-07 2011-01-05 Santaris Pharma A/S Pharmaceutical compositions for treatment of microrna related diseases
US8492357B2 (en) 2008-08-01 2013-07-23 Santaris Pharma A/S Micro-RNA mediated modulation of colony stimulating factors
US20100240049A1 (en) 2009-01-16 2010-09-23 Cepheid Methods of Detecting Cervical Cancer
CN102356163A (zh) * 2009-01-19 2012-02-15 西斯特米克苏格兰有限公司 采用非编码rna表达分析的方法
EP2391738B1 (en) 2009-02-02 2016-11-02 Cepheid Methods of detecting sepsis
WO2010085966A2 (en) * 2009-02-02 2010-08-05 Exiqon A/S Method for quantification of small rna species
EP2419537B1 (en) 2009-04-13 2014-01-29 Somagenics, Inc. Methods and compositions for detection of small rnas
US9034837B2 (en) 2009-04-24 2015-05-19 Roche Innovation Center Copenhagen A/S Pharmaceutical compositions for treatment of HCV patients that are poor-responders to interferon
US8563528B2 (en) 2009-07-21 2013-10-22 Santaris Pharma A/S Antisense oligomers targeting PCSK9
KR101822609B1 (ko) * 2010-06-24 2018-01-26 주식회사 파나진 Ｐｎａ 기반의 실시간 ｐｃｒ 클램핑을 이용한 마이크로ｒｎａ 발현양상의 분석 방법 및 키트
US9200314B2 (en) * 2010-07-21 2015-12-01 Takara Bio Inc. Synthetic siRNA detection method
WO2012103355A2 (en) 2011-01-26 2012-08-02 Cepheid Methods of detecting lung cancer
CN102154505B (zh) * 2011-04-20 2013-03-20 苟德明 一种检测miRNA的方法及引物及其应用
EP2794926B1 (en) 2011-12-22 2018-01-17 SomaGenics Inc. Methods of constructing small rna libraries and their use for expression profiling of target rnas
CN104271768A (zh) * 2012-02-14 2015-01-07 约翰霍普金斯大学 miRNA分析方法
US9163235B2 (en) 2012-06-21 2015-10-20 MiRagen Therapeutics, Inc. Inhibitors of the miR-15 family of micro-RNAs
WO2014071315A1 (en) 2012-11-02 2014-05-08 Life Technologies Corporation Novel compositions, methods and kits for enhancing pcr specificity
WO2014113614A1 (en) * 2013-01-16 2014-07-24 The Regents Of The University Of California Label free molecular detection methods, systems and devices
WO2014113598A2 (en) 2013-01-16 2014-07-24 The Regents Of The University Of California Microfluidic devices to extract, concentrate and isolate molecules
JP6479759B2 (ja) * 2013-03-14 2019-03-06 アーノルド, ライル, ジェイ.ARNOLD, Lyle, J. 固体支持体上での核酸増幅方法
CN103218543B (zh) * 2013-03-27 2016-04-13 中国科学院计算技术研究所 一种区分蛋白编码基因和非编码基因的方法及系统
MX367083B (es) 2013-06-27 2019-08-05 Roche Innovation Ct Copenhagen As Oligomeros antisentido y conjugados con diana en la proteina-convertasa subtilisina/kexina tipo 9(pcsk9).
CN104109708B (zh) * 2013-12-31 2015-05-13 厦门成坤生物技术有限公司 一种直接测定生物样品中未经分离的小核酸的方法及检测试剂盒
US10801067B2 (en) 2014-05-09 2020-10-13 Eurofins Lifecodexx Gmbh Detection of DNA that originates from a specific cell-type and related methods
EP2942400A1 (en) 2014-05-09 2015-11-11 Lifecodexx AG Multiplex detection of DNA that originates from a specific cell-type
EP4026914A1 (en) * 2014-10-08 2022-07-13 Cornell University Method for identification and relative quantification of nucleic acid methylation changes using combined nuclease, ligation, and polymerase reactions with carryover prevention
CN104598775B (zh) * 2014-12-31 2018-10-16 北京邮电大学 一种rna编辑事件识别方法
CN107429209B (zh) 2015-01-22 2021-05-07 爱科来株式会社 目标分析芯片以及目标分析方法
CN104673901B (zh) * 2015-02-06 2018-05-11 中国医学科学院基础医学研究所 用于检测靶核酸的用途和试剂盒
CN114250278B (zh) * 2015-03-13 2024-08-06 生命技术公司 捕获、检测和定量小rna的方法、组合物与试剂盒
CN104975078A (zh) * 2015-05-18 2015-10-14 上海市农业科学院 一种检测转基因植物中靶标siRNA池的方法
TWI676679B (zh) * 2015-06-02 2019-11-11 奎克生技光電股份有限公司 核苷酸序列、通用反向引子、通用反轉錄引子、引子設計方法及miRNA檢測方法
HUE050491T2 (hu) 2015-11-10 2020-12-28 Eurofins Lifecodexx Gmbh Magzati kromoszomális aneuploidiák kimutatása olyan DNS régiókat alkalmazva, amelyek különbözõképpen vannak metilezve a magzat és a terhes nõstény között
US11014957B2 (en) 2015-12-21 2021-05-25 Realseq Biosciences, Inc. Methods of library construction for polynucleotide sequencing
WO2017158840A1 (ja) 2016-03-18 2017-09-21 株式会社 東芝 試料中の複数種類の短鎖核酸の検出方法、組み合わせ分析キットおよび分析キット供給管理方法
CZ307055B6 (cs) * 2016-04-29 2017-12-20 Ústav molekulární genetiky AV ČR, v.v.i. Způsob detekce specifických mikroRNA pomocí templátové DNA a molekula templátové DNA pro použití při tomto způsobu
WO2019011875A1 (en) * 2017-07-11 2019-01-17 Qiagen Gmbh QUANTIFICATION OF ANTISENSE OLIGONUCLEOTIDES (ASOS)
EP3899035B1 (en) * 2018-12-20 2025-04-16 F. Hoffmann-La Roche AG Detection of target nucleic acid by solid-phase molography
WO2020239822A1 (en) * 2019-05-27 2020-12-03 The European Molecular Biology Laboratory Nucleic acid construct binding to influenza polymerase pb1 rna synthesis active site
US20220389497A1 (en) * 2019-11-04 2022-12-08 Mirnax Biosens, S.L. Bivalent reverse primer
EP3901286A1 (en) * 2020-04-24 2021-10-27 Mirnax Biosens, S.L. Bivalent reverse primer
CN111665351B (zh) * 2020-06-20 2021-12-03 江南大学 一种快速、特异性测定rna含量的方法
CN113322523B (zh) * 2021-06-17 2024-03-19 翌圣生物科技(上海)股份有限公司 Rna快速建库方法及其应用
CN116200492B (zh) * 2022-11-08 2025-04-01 浙江大学 一种基于探针锚定双链的小核酸检测试剂盒及其应用

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR20020034161A (ko) * 1999-07-19 2002-05-08 알. 씨. 제닝스 불충분한 핵산 서열의 증폭방법 및 그 방법의 수행장치
EP1353936A4 (en) * 2000-12-27 2005-05-18 Invitrogen Corp PRIMER AND METHOD FOR THE DETECTION AND DISTINCTION OF NUCLEIC ACIDS
US20040235005A1 (en) * 2002-10-23 2004-11-25 Ernest Friedlander Methods and composition for detecting targets
US7217807B2 (en) * 2002-11-26 2007-05-15 Rosetta Genomics Ltd Bioinformatically detectable group of novel HIV regulatory genes and uses thereof
JP4230457B2 (ja) * 2002-12-18 2009-02-25 サード・ウェーブ・テクノロジーズ・インク 低分子核酸の検出方法
WO2005003318A2 (en) * 2003-07-02 2005-01-13 Perkinelmer Las, Inc. Assay and process for labeling and detection of micro rna and small interfering rna sequences
US20080045417A1 (en) * 2003-10-14 2008-02-21 Jan Weiler Oligonucleotide Microarray

Also Published As

Publication number Publication date
WO2005098029A3 (en) 2005-12-15
CN1961081B (zh) 2012-09-05
CN1961081A (zh) 2007-05-09
ES2380923T3 (es) 2012-05-21
EP1735459B1 (en) 2012-01-25
DK1735459T3 (da) 2012-05-29
WO2005098029A2 (en) 2005-10-20
JP2007532100A (ja) 2007-11-15
CA2562390A1 (en) 2005-10-20
EP1735459A2 (en) 2006-12-27
JP5192229B2 (ja) 2013-05-08
ATE542918T1 (de) 2012-02-15

Similar Documents

Publication Publication Date Title
EP1735459B1 (en) Methods for quantification of micrornas and small interfering rnas
US8192937B2 (en) Methods for quantification of microRNAs and small interfering RNAs
WO2008040355A2 (en) Novel methods for quantification of micrornas and small interfering rnas
CA2750029C (en) Method for quantification of small rna species
KR100977186B1 (ko) 이중 특이성 올리고뉴클레오타이드를 사용한 방법 및 이중특이성 올리고뉴클레오타이드
KR20110122231A (ko) 이중 특이성 올리고뉴클레오타이드를 사용한 방법 및 이중 특이성 올리고뉴클레오타이드
CA2483930A1 (en) Methods for the enrichment of low-abundance polynucleotides
JP2011500092A (ja) 非ランダムプライマーを用いたｃＤＮＡ合成の方法
WO2006102309A2 (en) Methods, compositions, and kits for detection of micro rna
US20090023151A1 (en) Method For The Labeling And Detection Of Small Polynucleotides
US20020072061A1 (en) Methods and compositions for use in synthesizing nucleic acids
EP1639130B1 (en) Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same
WO2006066592A2 (en) Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same
WO2009029875A1 (en) Method for the labeling and detection of small polynucleotides
WO2005121358A2 (en) Extendable probes
HK1254039A1 (zh) 用於催化性测定的方法和组合物
HK1162196B (en) Method for quantification of small rna species

Legal Events

Date Code Title Description
EEER Examination request