CA2498636C - Utilisation de mutants de erg-4 de saccharomyces cerevisiae pour l'expression de transporteurs de glucose chez des mammiferes - Google Patents

Utilisation de mutants de erg-4 de saccharomyces cerevisiae pour l'expression de transporteurs de glucose chez des mammiferes Download PDF

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Publication number
CA2498636C
CA2498636C CA2498636A CA2498636A CA2498636C CA 2498636 C CA2498636 C CA 2498636C CA 2498636 A CA2498636 A CA 2498636A CA 2498636 A CA2498636 A CA 2498636A CA 2498636 C CA2498636 C CA 2498636C
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Canada
Prior art keywords
protein
yeast cell
yeast
polynucleotide
glucose
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Expired - Fee Related
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CA2498636A
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English (en)
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CA2498636A1 (fr
Inventor
Guenter Mueller
Silke Dlugai
Dorthe Voss
Eckhard Boles
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Sanofi Aventis Deutschland GmbH
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Sanofi Aventis Deutschland GmbH
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Publication of CA2498636A1 publication Critical patent/CA2498636A1/fr
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Publication of CA2498636C publication Critical patent/CA2498636C/fr
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/62—Insulins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/08—Drugs for disorders of the metabolism for glucose homeostasis
    • A61P3/10—Drugs for disorders of the metabolism for glucose homeostasis for hyperglycaemia, e.g. antidiabetics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Diabetes (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Endocrinology (AREA)
  • Hematology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Toxicology (AREA)
  • Emergency Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Obesity (AREA)
  • Plant Pathology (AREA)
  • Veterinary Medicine (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Public Health (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

L'invention concerne des souches de levure dans lesquelles un transporteur GLUT4 humain ou un transporteur GLUT1 humain peut être amené à expression de façon fonctionnelle, ainsi que des protéines de transport GLUT4 définies, pouvant être exprimées très simplement de façon fonctionnelle dans des souches de levure.
CA2498636A 2002-09-14 2003-09-04 Utilisation de mutants de erg-4 de saccharomyces cerevisiae pour l'expression de transporteurs de glucose chez des mammiferes Expired - Fee Related CA2498636C (fr)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10242763A DE10242763A1 (de) 2002-09-14 2002-09-14 Verwendung von Saccharomyces cerevisiae erg4-Mutanten zur Expression von Glukosetransportern aus Säugetieren
DE10242763.1 2002-09-14
PCT/EP2003/009812 WO2004026907A2 (fr) 2002-09-14 2003-09-04 Utilisation de mutants de erg-4 de saccharomyces cerevisiae pour l'expression de transporteurs de glucose chez des mammiferes

Publications (2)

Publication Number Publication Date
CA2498636A1 CA2498636A1 (fr) 2004-04-01
CA2498636C true CA2498636C (fr) 2012-04-17

Family

ID=31724774

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2498636A Expired - Fee Related CA2498636C (fr) 2002-09-14 2003-09-04 Utilisation de mutants de erg-4 de saccharomyces cerevisiae pour l'expression de transporteurs de glucose chez des mammiferes

Country Status (15)

Country Link
EP (1) EP1539958A2 (fr)
JP (1) JP4520854B2 (fr)
KR (1) KR101233998B1 (fr)
CN (1) CN1694960B (fr)
AU (1) AU2003264257B2 (fr)
BR (1) BR0314115A (fr)
CA (1) CA2498636C (fr)
DE (1) DE10242763A1 (fr)
HK (1) HK1084401A1 (fr)
IL (2) IL167321A (fr)
MX (1) MXPA05002816A (fr)
NO (1) NO20051795L (fr)
RU (1) RU2345136C2 (fr)
WO (1) WO2004026907A2 (fr)
ZA (1) ZA200501871B (fr)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102899328A (zh) * 2012-09-28 2013-01-30 南京农业大学 山羊葡萄糖转运载体4基因及其重组表达载体和应用
CN110408616B (zh) * 2019-07-09 2021-06-15 中南民族大学 GLUT4基因敲除的sgRNA、A549细胞系及其构建方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5942398A (en) * 1998-02-26 1999-08-24 Millennium Pharmaceuticals, Inc. Nucleic acid molecules encoding glutx and uses thereof
AU5477500A (en) * 1999-06-09 2000-12-28 General Hospital Corporation, The Method of measuring plasma membrane targeting of glut4
DE10106718A1 (de) * 2001-02-14 2002-09-05 Aventis Pharma Gmbh Hefestamm von Saccharomyces cerevisiae mit funktioneller Expression eines Glut-Transporters

Also Published As

Publication number Publication date
JP4520854B2 (ja) 2010-08-11
CN1694960A (zh) 2005-11-09
KR20050056206A (ko) 2005-06-14
KR101233998B1 (ko) 2013-02-18
WO2004026907A2 (fr) 2004-04-01
JP2006517088A (ja) 2006-07-20
IL167321A (en) 2013-11-28
HK1084401A1 (en) 2006-07-28
IL197621A (en) 2014-01-30
CA2498636A1 (fr) 2004-04-01
BR0314115A (pt) 2005-07-12
MXPA05002816A (es) 2005-05-27
AU2003264257A1 (en) 2004-04-08
CN1694960B (zh) 2010-05-12
WO2004026907A3 (fr) 2004-11-11
DE10242763A1 (de) 2004-03-18
AU2003264257B2 (en) 2010-05-20
RU2005110955A (ru) 2006-01-20
IL197621A0 (en) 2011-08-01
RU2345136C2 (ru) 2009-01-27
NO20051795L (no) 2005-06-08
EP1539958A2 (fr) 2005-06-15
ZA200501871B (en) 2005-10-26

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