CA2465283A1 - Novel sulfurylase-luciferase fusion proteins and thermostable sulfurylase - Google Patents

Novel sulfurylase-luciferase fusion proteins and thermostable sulfurylase Download PDF

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Publication number
CA2465283A1
CA2465283A1 CA002465283A CA2465283A CA2465283A1 CA 2465283 A1 CA2465283 A1 CA 2465283A1 CA 002465283 A CA002465283 A CA 002465283A CA 2465283 A CA2465283 A CA 2465283A CA 2465283 A1 CA2465283 A1 CA 2465283A1
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CA
Canada
Prior art keywords
atp
nucleic acid
polypeptide
group
sulfurylase
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
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CA002465283A
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English (en)
French (fr)
Inventor
Maithreyan Srinivasan
Michael Reifler
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454 Life Science Corp
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Individual
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Filing date
Publication date
Priority claimed from US10/122,706 external-priority patent/US6956114B2/en
Application filed by Individual filed Critical Individual
Publication of CA2465283A1 publication Critical patent/CA2465283A1/en
Abandoned legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004Oxidoreductases (1.)
    • C12N9/0069Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CA002465283A 2001-10-30 2002-10-30 Novel sulfurylase-luciferase fusion proteins and thermostable sulfurylase Abandoned CA2465283A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
US33594901P 2001-10-30 2001-10-30
US69/335,949 2001-10-30
US10/122,706 US6956114B2 (en) 2001-10-30 2002-04-11 Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US10/122,706 2002-04-11
US10/154,515 US6902921B2 (en) 2001-10-30 2002-05-23 Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US10/154,515 2002-05-23
PCT/US2002/034612 WO2003054142A2 (en) 2001-10-30 2002-10-30 Novel sulfurylase-luciferase fusion proteins and thermostable sulfurylase

Publications (1)

Publication Number Publication Date
CA2465283A1 true CA2465283A1 (en) 2003-07-03

Family

ID=27382840

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002465283A Abandoned CA2465283A1 (en) 2001-10-30 2002-10-30 Novel sulfurylase-luciferase fusion proteins and thermostable sulfurylase

Country Status (6)

Country Link
US (2) US6902921B2 (https=)
EP (2) EP1451293A4 (https=)
JP (2) JP2005517397A (https=)
AU (1) AU2002365146B2 (https=)
CA (1) CA2465283A1 (https=)
WO (1) WO2003054142A2 (https=)

Families Citing this family (115)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2003004690A2 (en) * 2001-07-06 2003-01-16 454$m(3) CORPORATION Method for isolation of independent, parallel chemical micro-reactions using a porous filter
CA2513899C (en) 2003-01-29 2013-03-26 454 Corporation Methods of amplifying and sequencing nucleic acids
DE10315640A1 (de) * 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
WO2005054431A2 (en) * 2003-12-01 2005-06-16 454 Corporation Method for isolation of independent, parallel chemical micro-reactions using a porous filter
GB0416944D0 (en) * 2004-07-29 2004-09-01 Lumora Ltd Method for determining the amount of template nucleic acid present in a sample
KR101234513B1 (ko) 2005-01-25 2013-02-19 야마사 쇼유 가부시키가이샤 3'-포스포아데노신-5'-포스포술페이트의 효소 합성법
DK2302070T3 (da) 2005-06-23 2012-11-26 Keygene Nv Strategier til identifikation og detektion af polymorfismer med højt gennemløb
JP2009513135A (ja) 2005-10-28 2009-04-02 プリーシス・ファーマシューティカルズ・インコーポレイテッド エンコードされたライブラリーを用いて興味のある化合物を同定する方法
JP5166276B2 (ja) * 2005-11-14 2013-03-21 ケイヘーネ・エヌ・ブイ トランスポゾンタギング集団のハイスループットスクリーニングおよび挿入部位の大規模並行配列特定のための方法
CN103937899B (zh) 2005-12-22 2017-09-08 凯津公司 用于基于aflp的高通量多态性检测的方法
US11237171B2 (en) 2006-02-21 2022-02-01 Trustees Of Tufts College Methods and arrays for target analyte detection and determination of target analyte concentration in solution
US8492098B2 (en) 2006-02-21 2013-07-23 The Trustees Of Tufts College Methods and arrays for target analyte detection and determination of reaction components that affect a reaction
US20090143658A1 (en) * 2006-02-27 2009-06-04 Edwards Lifesciences Corporation Analyte sensor
EP3239304B1 (en) 2006-04-04 2020-08-19 Keygene N.V. High throughput detection of molecular markers based on aflp and high troughput sequencing
US10522240B2 (en) 2006-05-03 2019-12-31 Population Bio, Inc. Evaluating genetic disorders
US7702468B2 (en) * 2006-05-03 2010-04-20 Population Diagnostics, Inc. Evaluating genetic disorders
EP4170042A1 (en) 2006-06-14 2023-04-26 Verinata Health, Inc. Methods for the diagnosis of fetal abnormalities
CN103333949B (zh) 2006-07-12 2015-05-06 凯津公司 使用aflp的高通量物理作图
GB2442048B (en) * 2006-07-25 2009-09-30 Proimmune Ltd Biotinylated MHC complexes and their uses
CN1995063B (zh) * 2006-12-30 2010-09-08 中国科学技术大学 一种植物硫高效利用蛋白及其编码基因与应用
WO2008106115A2 (en) 2007-02-26 2008-09-04 Monogram Biosciences, Inc. Compositions and methods for determining whether a subject would benefit from co-receptor inhibitor therapy
JP2010537643A (ja) 2007-08-29 2010-12-09 アプライド バイオシステムズ, エルエルシー 代替的な核酸配列決定法
JP5503540B2 (ja) 2007-08-30 2014-05-28 トラスティーズ・オブ・タフツ・カレッジ 溶液中の分析物濃度を決定する方法
US20090181390A1 (en) * 2008-01-11 2009-07-16 Signosis, Inc. A California Corporation High throughput detection of micrornas and use for disease diagnosis
CA2718905A1 (en) 2008-03-17 2009-09-24 Expressive Research B.V. Expression-linked gene discovery
JP2010029146A (ja) * 2008-07-30 2010-02-12 Hitachi Ltd 塩基配列解析法
US8222047B2 (en) 2008-09-23 2012-07-17 Quanterix Corporation Ultra-sensitive detection of molecules on single molecule arrays
EP2373790B1 (en) 2008-12-05 2015-02-11 Keygene N.V. Farnesene synthase
WO2010082815A1 (en) 2009-01-13 2010-07-22 Keygene N.V. Novel genome sequencing strategies
US10174368B2 (en) 2009-09-10 2019-01-08 Centrillion Technology Holdings Corporation Methods and systems for sequencing long nucleic acids
CN102858995B (zh) 2009-09-10 2016-10-26 森特瑞隆技术控股公司 靶向测序方法
JP2013509883A (ja) 2009-11-06 2013-03-21 ザ ボード オブ トラスティーズ オブ ザ リーランド スタンフォード ジュニア ユニバーシティ 臓器移植患者における移植片拒絶反応の非侵襲的診断方法
WO2011071382A1 (en) 2009-12-10 2011-06-16 Keygene N.V. Polymorfphic whole genome profiling
EP2513333B1 (en) 2009-12-17 2013-10-02 Keygene N.V. Restriction enzyme based whole genome sequencing
AU2010330940A1 (en) 2009-12-18 2012-08-02 Keygene N.V. Improved bulked mutant analysis
CN103026232B (zh) 2010-03-01 2015-02-04 匡特里克斯公司 扩大用于检测分子或颗粒的测定法中的动态范围的方法和系统
US8236574B2 (en) 2010-03-01 2012-08-07 Quanterix Corporation Ultra-sensitive detection of molecules or particles using beads or other capture objects
US8415171B2 (en) 2010-03-01 2013-04-09 Quanterix Corporation Methods and systems for extending dynamic range in assays for the detection of molecules or particles
US9678068B2 (en) 2010-03-01 2017-06-13 Quanterix Corporation Ultra-sensitive detection of molecules using dual detection methods
EP3540059A1 (en) 2010-04-16 2019-09-18 Nuevolution A/S Bi-functional complexes and methods for making and using such complexes
WO2012008831A1 (en) 2010-07-13 2012-01-19 Keygene N.V. Simplified de novo physical map generation from clone libraries
WO2012018387A2 (en) 2010-08-02 2012-02-09 Population Diagnotics, Inc. Compositions and methods for discovery of causative mutations in genetic disorders
US20120070829A1 (en) 2010-09-10 2012-03-22 Bio-Rad Laboratories, Inc. Size selection of dna for chromatin analysis
CA2823815A1 (en) 2011-01-14 2012-07-19 Keygene N.V. Paired end random sequence based genotyping
US9952237B2 (en) 2011-01-28 2018-04-24 Quanterix Corporation Systems, devices, and methods for ultra-sensitive detection of molecules or particles
US20130316358A1 (en) 2011-01-31 2013-11-28 Yeda Research And Development Co. Ltd. Methods of diagnosing disease using overlap extension pcr
WO2012106385A2 (en) 2011-01-31 2012-08-09 Apprise Bio, Inc. Methods of identifying multiple epitopes in cells
US20120252682A1 (en) 2011-04-01 2012-10-04 Maples Corporate Services Limited Methods and systems for sequencing nucleic acids
WO2012142301A2 (en) 2011-04-12 2012-10-18 Quanterix Corporation Methods of determining a treatment protocol for and/or a prognosis of a patients recovery from a brain injury
EP3000870B1 (en) * 2011-04-22 2018-01-24 Danisco US Inc. Filamentous fungi having an altered viscosity phenotype
JP6164657B2 (ja) 2011-06-10 2017-07-19 シュティッヒティング・テクノロジッシュ・トップインスティテュート・グレーネ・ジェネティカ・(ファウンデーション・テクノロジカル・トップ・インスティテュート・グリーン・ジェネティクス) テルペン生合成を調節する転写因子
DK2729580T3 (en) 2011-07-08 2015-12-14 Keygene Nv SEQUENCE BASED genotyping BASED ON OLIGONUKLEOTIDLIGERINGSASSAYS
EP2766483B1 (en) 2011-10-10 2022-03-23 The Hospital For Sick Children Methods and compositions for screening and treating developmental disorders
EP2773779B1 (en) 2011-11-04 2020-10-14 Population Bio, Inc. Methods and compositions for diagnosing, prognosing, and treating neurological conditions
EP3836149A1 (en) 2011-11-07 2021-06-16 QIAGEN Redwood City, Inc. Methods and systems for identification of causal genomic variants
EP2802666B1 (en) 2012-01-13 2018-09-19 Data2Bio Genotyping by next-generation sequencing
US10407724B2 (en) 2012-02-09 2019-09-10 The Hospital For Sick Children Methods and compositions for screening and treating developmental disorders
EP2882868B1 (en) 2012-08-08 2019-07-31 H. Hoffnabb-La Roche Ag Increasing dynamic range for identifying multiple epitopes in cells
DK2895621T3 (da) 2012-09-14 2020-11-30 Population Bio Inc Fremgangsmåder og sammensætning til diagnosticering, prognose og behandling af neurologiske tilstande
WO2014052855A1 (en) 2012-09-27 2014-04-03 Population Diagnostics, Inc. Methods and compositions for screening and treating developmental disorders
US9932626B2 (en) 2013-01-15 2018-04-03 Quanterix Corporation Detection of DNA or RNA using single molecule arrays and other techniques
WO2014121091A1 (en) 2013-02-01 2014-08-07 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
US9411930B2 (en) 2013-02-01 2016-08-09 The Regents Of The University Of California Methods for genome assembly and haplotype phasing
ES2662598T3 (es) 2013-03-08 2018-04-09 F. Hoffmann-La Roche Ag Análisis de sangre para la detección de mutaciones de EGFR
WO2014151117A1 (en) 2013-03-15 2014-09-25 The Board Of Trustees Of The Leland Stanford Junior University Identification and use of circulating nucleic acid tumor markers
EP3611262B1 (en) 2013-03-15 2020-11-11 Lineage Biosciences, Inc. Methods of sequencing the immune repertoire
SG11201600853UA (en) 2013-08-05 2016-03-30 Twist Bioscience Corp De novo synthesized gene libraries
AU2014346562B2 (en) 2013-11-07 2018-11-29 The Board Of Trustees Of The Leland Stanford Junior University Cell-free nucleic acids for the analysis of the human microbiome and components thereof
DK3511422T3 (da) 2013-11-12 2023-02-06 Population Bio Inc Fremgangsmåder og sammensætninger til diagnosticering, prognose og behandling af endometriose
EP3540074A1 (en) 2013-12-11 2019-09-18 The Regents of the University of California Method of tagging internal regions of nucleic acid molecules
US10526641B2 (en) 2014-08-01 2020-01-07 Dovetail Genomics, Llc Tagging nucleic acids for sequence assembly
GB2558326B (en) 2014-09-05 2021-01-20 Population Bio Inc Methods and compositions for inhibiting and treating neurological conditions
EP3237616A1 (en) 2014-12-24 2017-11-01 Keygene N.V. Backbone mediated mate pair sequencing
CA2975855C (en) 2015-02-04 2025-09-23 Twist Bioscience Corporation SYNTHETIC GENE COMPOSITIONS AND ASSEMBLY METHODS
US10669304B2 (en) 2015-02-04 2020-06-02 Twist Bioscience Corporation Methods and devices for de novo oligonucleic acid assembly
WO2016134034A1 (en) 2015-02-17 2016-08-25 Dovetail Genomics Llc Nucleic acid sequence assembly
US11807896B2 (en) 2015-03-26 2023-11-07 Dovetail Genomics, Llc Physical linkage preservation in DNA storage
US9981239B2 (en) 2015-04-21 2018-05-29 Twist Bioscience Corporation Devices and methods for oligonucleic acid library synthesis
EP3350314A4 (en) 2015-09-18 2019-02-06 Twist Bioscience Corporation OLIGONUCLEIC ACID VARIANT LIBRARIES VARIANT AND SYNTHESIS THEREOF
KR102794025B1 (ko) 2015-09-22 2025-04-09 트위스트 바이오사이언스 코포레이션 핵산 합성을 위한 가요성 기판
US10465232B1 (en) 2015-10-08 2019-11-05 Trace Genomics, Inc. Methods for quantifying efficiency of nucleic acid extraction and detection
CA3002740A1 (en) 2015-10-19 2017-04-27 Dovetail Genomics, Llc Methods for genome assembly, haplotype phasing, and target independent nucleic acid detection
CN115920796A (zh) 2015-12-01 2023-04-07 特韦斯特生物科学公司 功能化表面及其制备
SG11201807117WA (en) 2016-02-23 2018-09-27 Dovetail Genomics Llc Generation of phased read-sets for genome assembly and haplotype phasing
EP3978627A1 (en) 2016-03-25 2022-04-06 Karius, Inc. Methods using synthetic nucleic acid spike-ins
KR102412442B1 (ko) 2016-05-13 2022-06-22 더브테일 제노믹스 엘엘씨 보존된 샘플로부터의 장범위 링키지 정보의 회수
EP3500672A4 (en) 2016-08-22 2020-05-20 Twist Bioscience Corporation DE NOVO SYNTHETIZED NUCLEIC ACID LIBRARIES
US10417457B2 (en) 2016-09-21 2019-09-17 Twist Bioscience Corporation Nucleic acid based data storage
US11725232B2 (en) 2016-10-31 2023-08-15 The Hong Kong University Of Science And Technology Compositions, methods and kits for detection of genetic variants for alzheimer's disease
GB2573069A (en) 2016-12-16 2019-10-23 Twist Bioscience Corp Variant libraries of the immunological synapse and synthesis thereof
US10240205B2 (en) 2017-02-03 2019-03-26 Population Bio, Inc. Methods for assessing risk of developing a viral disease using a genetic test
EP4556433A3 (en) 2017-02-22 2025-08-06 Twist Bioscience Corporation Nucleic acid based data storage
WO2018170169A1 (en) 2017-03-15 2018-09-20 Twist Bioscience Corporation Variant libraries of the immunological synapse and synthesis thereof
WO2018191563A1 (en) 2017-04-12 2018-10-18 Karius, Inc. Sample preparation methods, systems and compositions
US20210371918A1 (en) 2017-04-18 2021-12-02 Dovetail Genomics, Llc Nucleic acid characteristics as guides for sequence assembly
WO2018231872A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
WO2018231864A1 (en) 2017-06-12 2018-12-20 Twist Bioscience Corporation Methods for seamless nucleic acid assembly
CA3068273A1 (en) 2017-06-21 2018-12-27 Bluedot Llc Systems and methods for identification of nucleic acids in a sample
US11407837B2 (en) 2017-09-11 2022-08-09 Twist Bioscience Corporation GPCR binding proteins and synthesis thereof
WO2019060716A1 (en) 2017-09-25 2019-03-28 Freenome Holdings, Inc. SAMPLE EXTRACTION METHODS AND SYSTEMS
CN111565834B (zh) 2017-10-20 2022-08-26 特韦斯特生物科学公司 用于多核苷酸合成的加热的纳米孔
KR102804057B1 (ko) 2018-01-04 2025-05-07 트위스트 바이오사이언스 코포레이션 Dna 기반 디지털 정보 저장
EP3746566A1 (en) 2018-01-31 2020-12-09 Dovetail Genomics, LLC Sample prep for dna linkage recovery
CA3100739A1 (en) 2018-05-18 2019-11-21 Twist Bioscience Corporation Polynucleotides, reagents, and methods for nucleic acid hybridization
EP4177356B1 (en) 2018-08-08 2024-05-08 PML Screening, LLC Methods for assessing risk of developing a viral disease using a genetic test
WO2020139871A1 (en) 2018-12-26 2020-07-02 Twist Bioscience Corporation Highly accurate de novo polynucleotide synthesis
JP2022522668A (ja) 2019-02-26 2022-04-20 ツイスト バイオサイエンス コーポレーション 抗体を最適化するための変異体核酸ライブラリ
CN113766930B (zh) 2019-02-26 2025-07-22 特韦斯特生物科学公司 Glp1受体的变异核酸文库
US11332738B2 (en) 2019-06-21 2022-05-17 Twist Bioscience Corporation Barcode-based nucleic acid sequence assembly
EP4545965A3 (en) 2019-06-27 2025-06-11 Dovetail Genomics, LLC Methods and compositions for proximity ligation
EP4034566A4 (en) 2019-09-23 2024-01-24 Twist Bioscience Corporation VARIANT NUCLEIC ACID BANKS FOR CRTH2
JP2022548783A (ja) 2019-09-23 2022-11-21 ツイスト バイオサイエンス コーポレーション 単一ドメイン抗体のバリアント核酸ライブラリー
BR112022011235A2 (pt) 2019-12-09 2022-12-13 Twist Bioscience Corp Bibliotecas de variantes de ácido nucleico para receptores de adenosina
WO2024123733A1 (en) 2022-12-05 2024-06-13 Twist Bioscience Corporation Enzymes for library preparation
AU2024259004A1 (en) 2023-04-21 2025-12-04 Twist Bioscience Corporation Polymerase variants

Family Cites Families (106)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4863849A (en) 1985-07-18 1989-09-05 New York Medical College Automatable process for sequencing nucleotide
DE122007000007I2 (de) 1986-04-09 2010-12-30 Genzyme Corp Genetisch transformierte Tiere, die ein gewünschtes Protein in Milch absondern
US4822746A (en) 1986-06-25 1989-04-18 Trustees Of Tufts College Radiative and non-radiative energy transfer and absorbance modulated fluorescence detection methods and sensors
US5252494A (en) 1986-06-25 1993-10-12 Trustees Of Tufts College Fiber optic sensors, apparatus, and detection methods using controlled release polymers and reagent formulations held within a polymeric reaction matrix
US5143853A (en) 1986-06-25 1992-09-01 Trustees Of Tufts College Absorbance modulated fluorescence detection methods and sensors
US5254477A (en) 1986-06-25 1993-10-19 Trustees Of Tufts College Flourescence intramolecular energy transfer conjugate compositions and detection methods
US4873316A (en) 1987-06-23 1989-10-10 Biogen, Inc. Isolation of exogenous recombinant proteins from the milk of transgenic mammals
GB8810400D0 (en) 1988-05-03 1988-06-08 Southern E Analysing polynucleotide sequences
SE8801070D0 (sv) 1988-03-23 1988-03-23 Pharmacia Ab Method for immobilizing a dna sequence on a solid support
US4971903A (en) 1988-03-25 1990-11-20 Edward Hyman Pyrophosphate-based method and apparatus for sequencing nucleic acids
US6054270A (en) 1988-05-03 2000-04-25 Oxford Gene Technology Limited Analying polynucleotide sequences
US5700637A (en) 1988-05-03 1997-12-23 Isis Innovation Limited Apparatus and method for analyzing polynucleotide sequences and method of generating oligonucleotide arrays
GB8822228D0 (en) 1988-09-21 1988-10-26 Southern E M Support-bound oligonucleotides
US5143854A (en) 1989-06-07 1992-09-01 Affymax Technologies N.V. Large scale photolithographic solid phase synthesis of polypeptides and receptor binding screening thereof
US5800992A (en) 1989-06-07 1998-09-01 Fodor; Stephen P.A. Method of detecting nucleic acids
US5744101A (en) 1989-06-07 1998-04-28 Affymax Technologies N.V. Photolabile nucleoside protecting groups
US5871928A (en) 1989-06-07 1999-02-16 Fodor; Stephen P. A. Methods for nucleic acid analysis
US5545522A (en) 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
US5244636A (en) 1991-01-25 1993-09-14 Trustees Of Tufts College Imaging fiber optic array sensors, apparatus, and methods for concurrently detecting multiple analytes of interest in a fluid sample
US5250264A (en) 1991-01-25 1993-10-05 Trustees Of Tufts College Method of making imaging fiber optic sensors to concurrently detect multiple analytes of interest in a fluid sample
US5320814A (en) 1991-01-25 1994-06-14 Trustees Of Tufts College Fiber optic array sensors, apparatus, and methods for concurrently visualizing and chemically detecting multiple analytes of interest in a fluid sample
US5114984A (en) 1991-04-26 1992-05-19 Olin Corporation Process for producing an antimicrobially effective polyurethane
CA2124087C (en) 1991-11-22 2002-10-01 James L. Winkler Combinatorial strategies for polymer synthesis
GB9210176D0 (en) 1992-05-12 1992-06-24 Cemu Bioteknik Ab Chemical method
US6114114A (en) 1992-07-17 2000-09-05 Incyte Pharmaceuticals, Inc. Comparative gene transcript analysis
US5298741A (en) 1993-01-13 1994-03-29 Trustees Of Tufts College Thin film fiber optic sensor array and apparatus for concurrent viewing and chemical sensing of a sample
US5714320A (en) 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
GB9315847D0 (en) 1993-07-30 1993-09-15 Isis Innovation Tag reagent and assay method
US5429807A (en) 1993-10-28 1995-07-04 Beckman Instruments, Inc. Method and apparatus for creating biopolymer arrays on a solid support surface
GB9401833D0 (en) 1994-02-01 1994-03-30 Isis Innovation Method for discovering ligands
US5512490A (en) 1994-08-11 1996-04-30 Trustees Of Tufts College Optical sensor, optical sensing apparatus, and methods for detecting an analyte of interest using spectral recognition patterns
US6013445A (en) 1996-06-06 2000-01-11 Lynx Therapeutics, Inc. Massively parallel signature sequencing by ligation of encoded adaptors
US5795716A (en) 1994-10-21 1998-08-18 Chee; Mark S. Computer-aided visualization and analysis system for sequence evaluation
GB9507238D0 (en) 1995-04-07 1995-05-31 Isis Innovation Detecting dna sequence variations
US5750341A (en) 1995-04-17 1998-05-12 Lynx Therapeutics, Inc. DNA sequencing by parallel oligonucleotide extensions
US5648245A (en) 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
US6200737B1 (en) 1995-08-24 2001-03-13 Trustees Of Tufts College Photodeposition method for fabricating a three-dimensional, patterned polymer microstructure
US5843655A (en) 1995-09-18 1998-12-01 Affymetrix, Inc. Methods for testing oligonucleotide arrays
US5780231A (en) 1995-11-17 1998-07-14 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
US5962228A (en) 1995-11-17 1999-10-05 Lynx Therapeutics, Inc. DNA extension and analysis with rolling primers
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US5633972A (en) 1995-11-29 1997-05-27 Trustees Of Tufts College Superresolution imaging fiber for subwavelength light energy generation and near-field optical microscopy
US5814524A (en) 1995-12-14 1998-09-29 Trustees Of Tufts College Optical sensor apparatus for far-field viewing and making optical analytical measurements at remote locations
US5744341A (en) * 1996-03-29 1998-04-28 University Of Maryland College Park Genes of carotenoid biosynthesis and metabolism and a system for screening for such genes
US6040413A (en) * 1996-07-10 2000-03-21 Basf Corporation Composition of polytetramethylene ether glycols and polyoxy alkylene polyether polyols having a low degree of unsaturation
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
US5900481A (en) 1996-11-06 1999-05-04 Sequenom, Inc. Bead linkers for immobilizing nucleic acids to solid supports
US6133436A (en) 1996-11-06 2000-10-17 Sequenom, Inc. Beads bound to a solid support and to nucleic acids
US6887665B2 (en) 1996-11-14 2005-05-03 Affymetrix, Inc. Methods of array synthesis
GB9626815D0 (en) 1996-12-23 1997-02-12 Cemu Bioteknik Ab Method of sequencing DNA
US6136543A (en) 1997-01-31 2000-10-24 Hitachi, Ltd. Method for determining nucleic acids base sequence and apparatus therefor
WO1998035012A2 (en) 1997-02-12 1998-08-13 Chan Eugene Y Methods and products for analyzing polymers
US6023540A (en) 1997-03-14 2000-02-08 Trustees Of Tufts College Fiber optic sensor with encoded microspheres
GB9707486D0 (en) * 1997-04-11 1997-05-28 Secr Defence Enzyme assays
JP4294740B2 (ja) 1997-05-23 2009-07-15 ソレクサ・インコーポレイテッド 分析物の系列的プロセシングのためのシステムおよび装置
JP2001511358A (ja) 1997-07-28 2001-08-14 メディカル・バイオシステムズ・リミテッド 核酸配列の解析
US20010006630A1 (en) 1997-09-02 2001-07-05 Oron Yacoby-Zeevi Introducing a biological material into a patient
DE69822206T2 (de) 1997-12-19 2005-02-17 Affymetrix, Inc., Santa Clara Erkenntnisse der genomforschung für die suche nach neuartigen wirkstoffen
US6210910B1 (en) 1998-03-02 2001-04-03 Trustees Of Tufts College Optical fiber biosensor array comprising cell populations confined to microcavities
ATE256142T1 (de) 1998-05-15 2003-12-15 Isis Innovation Bibliotheken von unterschiedlich markierten oligomeren
GB9811403D0 (en) 1998-05-27 1998-07-22 Isis Innovation Polynucleotide multimers and their use in hybridisation assays
GB9813216D0 (en) 1998-06-18 1998-08-19 Pyrosequencing Ab Reaction monitoring systems
EP1090293B2 (en) 1998-06-24 2019-01-23 Illumina, Inc. Decoding of array sensors with microspheres
WO2000006770A1 (en) 1998-07-30 2000-02-10 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6210896B1 (en) 1998-08-13 2001-04-03 Us Genomics Molecular motors
US6263286B1 (en) 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
US6203989B1 (en) 1998-09-30 2001-03-20 Affymetrix, Inc. Methods and compositions for amplifying detectable signals in specific binding assays
EP1131153A1 (en) 1998-11-06 2001-09-12 Solexa Ltd. A method for reproducing molecular arrays
US6429027B1 (en) 1998-12-28 2002-08-06 Illumina, Inc. Composite arrays utilizing microspheres
GB9901475D0 (en) 1999-01-22 1999-03-17 Pyrosequencing Ab A method of DNA sequencing
CA2361223A1 (en) 1999-01-29 2000-08-03 Illumina, Inc. Centrifuging apparatus and method for separation of liquid phases and organic synthesis
ATE462970T1 (de) 1999-02-09 2010-04-15 Illumina Inc Screeningverfahren mit porösen mikrokügelchen und zusammensetzungen
CA2359352C (en) 1999-02-09 2004-09-21 Illumina, Inc. Arrays comprising a fiducial and automated information processing in randomly ordered arrays
US6255476B1 (en) 1999-02-22 2001-07-03 Pe Corporation (Ny) Methods and compositions for synthesis of labelled oligonucleotides and analogs on solid-supports
GB9906477D0 (en) 1999-03-19 1999-05-12 Pyrosequencing Ab Liquid dispensing apparatus
WO2000058507A1 (en) 1999-03-30 2000-10-05 Solexa Ltd. Polynucleotide sequencing
GB9907813D0 (en) 1999-04-06 1999-06-02 Medical Biosystems Ltd Synthesis
GB9907812D0 (en) 1999-04-06 1999-06-02 Medical Biosystems Ltd Sequencing
WO2000063437A2 (en) 1999-04-20 2000-10-26 Illumina, Inc. Detection of nucleic acid reactions on bead arrays
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US6221653B1 (en) 1999-04-27 2001-04-24 Agilent Technologies, Inc. Method of performing array-based hybridization assays using thermal inkjet deposition of sample fluids
US6544732B1 (en) 1999-05-20 2003-04-08 Illumina, Inc. Encoding and decoding of array sensors utilizing nanocrystals
AU779835B2 (en) 1999-05-20 2005-02-10 Illumina, Inc. Method and apparatus for retaining and presenting at least one microsphere array to solutions and/or to optical imaging systems
EP1190100B1 (en) 1999-05-20 2012-07-25 Illumina, Inc. Combinatorial decoding of random nucleic acid arrays
US20020051971A1 (en) 1999-05-21 2002-05-02 John R. Stuelpnagel Use of microfluidic systems in the detection of target analytes using microsphere arrays
WO2001018524A2 (en) 1999-08-30 2001-03-15 Illumina, Inc. Methods for improving signal detection from an array
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
GB9923324D0 (en) 1999-10-01 1999-12-08 Pyrosequencing Ab Separation apparatus and method
GB9923644D0 (en) 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
GB9929381D0 (en) 1999-12-10 2000-02-09 Pyrosequencing Ab A method of assessing the amount of nucleic acid in a sample
CA2393658A1 (en) 1999-12-23 2001-06-28 Illumina, Inc. Decoding of array sensors with microspheres
DK1259643T3 (da) 2000-02-07 2009-02-23 Illumina Inc Fremgangsmåde til detektion af nukleinsyre ved anvendelse af universel priming
WO2001057268A2 (en) 2000-02-07 2001-08-09 Illumina, Inc. Nucleic acid detection methods using universal priming
AU2001239760B2 (en) 2000-02-10 2005-11-24 Illumina, Inc. Array of individual arrays as substrate for bead-based simultaneous processing of samples and manufacturing method therefor
ATE412774T1 (de) 2000-02-16 2008-11-15 Illumina Inc Parallele genotypisierung mehrerer patientenproben
SE0001768D0 (sv) 2000-05-12 2000-05-12 Helen Andersson Mikrofluidisk flödescell för manipulering av partiklar
CA2677953C (en) 2000-06-28 2011-04-26 Illumina, Inc. Composite arrays utilizing microspheres with a hybridization chamber
EP1379995A2 (en) 2000-08-09 2004-01-14 Illumina, Inc. Automated information processing in randomly ordered arrays
WO2002016649A2 (en) 2000-08-25 2002-02-28 Illumina, Inc. Probes and decoder oligonucleotides
US20050158702A1 (en) 2000-09-05 2005-07-21 Stuelpnagel John R. Cellular arrays comprising encoded cells
GB0021977D0 (en) 2000-09-07 2000-10-25 Pyrosequencing Ab Method of sequencing DNA
GB0022069D0 (en) 2000-09-08 2000-10-25 Pyrosequencing Ab Method
US10071944B2 (en) 2014-06-20 2018-09-11 P2 Science, Inc. Film ozonolysis in a tubular or multitubular reactor

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