CA2396534C - Intrabodies with defined framework that is stable in a reducing environment and applications thereof - Google Patents

Intrabodies with defined framework that is stable in a reducing environment and applications thereof Download PDF

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Publication number
CA2396534C
CA2396534C CA2396534A CA2396534A CA2396534C CA 2396534 C CA2396534 C CA 2396534C CA 2396534 A CA2396534 A CA 2396534A CA 2396534 A CA2396534 A CA 2396534A CA 2396534 C CA2396534 C CA 2396534C
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CA
Canada
Prior art keywords
scfv
protein
stable
library
soluble
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2396534A
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English (en)
French (fr)
Other versions
CA2396534A1 (en
Inventor
Adrian Auf Der Maur
Alcide Barberis
Dominik Escher
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Esbatech a Novartis Co LLC
Original Assignee
Esbatech An Alcon Biomedical Research Unit LLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Esbatech An Alcon Biomedical Research Unit LLC filed Critical Esbatech An Alcon Biomedical Research Unit LLC
Publication of CA2396534A1 publication Critical patent/CA2396534A1/en
Application granted granted Critical
Publication of CA2396534C publication Critical patent/CA2396534C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/005—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies constructed by phage libraries
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/14—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from fungi, algea or lichens
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1055—Protein x Protein interaction, e.g. two hybrid selection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/505—Medicinal preparations containing antigens or antibodies comprising antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
    • C07K2317/622—Single chain antibody (scFv)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/80—Immunoglobulins specific features remaining in the (producing) cell, i.e. intracellular antibodies or intrabodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2318/00—Antibody mimetics or scaffolds
    • C07K2318/10—Immunoglobulin or domain(s) thereof as scaffolds for inserted non-Ig peptide sequences, e.g. for vaccination purposes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Virology (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Battery Electrode And Active Subsutance (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Stored Programmes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Catalysts (AREA)
CA2396534A 1999-12-28 2000-12-18 Intrabodies with defined framework that is stable in a reducing environment and applications thereof Expired - Fee Related CA2396534C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
IB9902054 1999-12-28
IBPCT/IB99/02054 1999-12-28
IBPCT/IB00/00218 2000-03-01
IBPCT/IB00/00218 2000-03-01
PCT/IB2000/001892 WO2001048017A1 (en) 1999-12-28 2000-12-18 Intrabodies with defined framework that is stable in a reducing environment and applications thereof

Publications (2)

Publication Number Publication Date
CA2396534A1 CA2396534A1 (en) 2001-07-05
CA2396534C true CA2396534C (en) 2012-10-02

Family

ID=26318608

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2396534A Expired - Fee Related CA2396534C (en) 1999-12-28 2000-12-18 Intrabodies with defined framework that is stable in a reducing environment and applications thereof

Country Status (10)

Country Link
US (3) US7258986B2 (https=)
EP (3) EP1242457B1 (https=)
JP (4) JP4698109B2 (https=)
AT (1) ATE273323T1 (https=)
AU (1) AU768827B2 (https=)
CA (1) CA2396534C (https=)
DE (1) DE60012980T2 (https=)
DK (1) DK1242457T3 (https=)
ES (1) ES2225269T3 (https=)
WO (1) WO2001048017A1 (https=)

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Also Published As

Publication number Publication date
US7258986B2 (en) 2007-08-21
JP5783703B2 (ja) 2015-09-24
WO2001048017A1 (en) 2001-07-05
DE60012980T2 (de) 2005-08-18
JP4698109B2 (ja) 2011-06-08
US20030096306A1 (en) 2003-05-22
AU1725201A (en) 2001-07-09
EP1479694A2 (en) 2004-11-24
DE60012980D1 (de) 2004-09-16
JP2013224294A (ja) 2013-10-31
US7258985B2 (en) 2007-08-21
EP1242457B1 (en) 2004-08-11
US20010024831A1 (en) 2001-09-27
DK1242457T3 (da) 2004-12-20
JP2011062205A (ja) 2011-03-31
US20080233110A1 (en) 2008-09-25
ES2225269T3 (es) 2005-03-16
CA2396534A1 (en) 2001-07-05
JP2016020388A (ja) 2016-02-04
EP2392596A2 (en) 2011-12-07
AU768827B2 (en) 2004-01-08
EP1479694A3 (en) 2010-05-05
EP2392596A3 (en) 2013-11-27
EP1242457A1 (en) 2002-09-25
EP1479694B1 (en) 2016-10-26
ATE273323T1 (de) 2004-08-15
JP2004504007A (ja) 2004-02-12

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