CA2374610A1 - Catalytically active recombinant memapsin and methods of use thereof - Google Patents
Catalytically active recombinant memapsin and methods of use thereof Download PDFInfo
- Publication number
- CA2374610A1 CA2374610A1 CA002374610A CA2374610A CA2374610A1 CA 2374610 A1 CA2374610 A1 CA 2374610A1 CA 002374610 A CA002374610 A CA 002374610A CA 2374610 A CA2374610 A CA 2374610A CA 2374610 A1 CA2374610 A1 CA 2374610A1
- Authority
- CA
- Canada
- Prior art keywords
- memapsin
- substrate
- leu
- ala
- inhibitors
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
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Classifications
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- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
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- C07K5/06017—Dipeptides with the first amino acid being neutral and aliphatic
- C07K5/06026—Dipeptides with the first amino acid being neutral and aliphatic the side chain containing 0 or 1 carbon atom, i.e. Gly or Ala
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
- C07K5/06—Dipeptides
- C07K5/06008—Dipeptides with the first amino acid being neutral
- C07K5/06017—Dipeptides with the first amino acid being neutral and aliphatic
- C07K5/06034—Dipeptides with the first amino acid being neutral and aliphatic the side chain containing 2 to 4 carbon atoms
- C07K5/06043—Leu-amino acid
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
- C07K5/08—Tripeptides
- C07K5/0802—Tripeptides with the first amino acid being neutral
- C07K5/0804—Tripeptides with the first amino acid being neutral and aliphatic
- C07K5/0806—Tripeptides with the first amino acid being neutral and aliphatic the side chain containing 0 or 1 carbon atoms, i.e. Gly, Ala
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
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- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2299/00—Coordinates from 3D structures of peptides, e.g. proteins or enzymes
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
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- Y02A90/10—Information and communication technologies [ICT] supporting adaptation to climate change, e.g. for weather forecasting or climate simulation
Landscapes
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- Pharmacology & Pharmacy (AREA)
- Psychiatry (AREA)
- Hospice & Palliative Care (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Applications Claiming Priority (11)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US14136399P | 1999-06-28 | 1999-06-28 | |
| US60/141,363 | 1999-06-28 | ||
| US16806099P | 1999-11-30 | 1999-11-30 | |
| US60/168,060 | 1999-11-30 | ||
| US17783600P | 2000-01-25 | 2000-01-25 | |
| US60/177,836 | 2000-01-25 | ||
| US17836800P | 2000-01-27 | 2000-01-27 | |
| US60/178,368 | 2000-01-27 | ||
| US21029200P | 2000-06-08 | 2000-06-08 | |
| US60/210,292 | 2000-06-08 | ||
| PCT/US2000/017661 WO2001000663A2 (en) | 1999-06-28 | 2000-06-27 | Catalytically active recombinant memapsin and methods of use thereof |
Publications (1)
| Publication Number | Publication Date |
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| CA2374610A1 true CA2374610A1 (en) | 2001-01-04 |
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| CA2374346A Expired - Fee Related CA2374346C (en) | 1999-06-28 | 2000-06-27 | Inhibitors of memapsin 2 and use thereof |
| CA002374610A Abandoned CA2374610A1 (en) | 1999-06-28 | 2000-06-27 | Catalytically active recombinant memapsin and methods of use thereof |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA2374346A Expired - Fee Related CA2374346C (en) | 1999-06-28 | 2000-06-27 | Inhibitors of memapsin 2 and use thereof |
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| Country | Link |
|---|---|
| US (7) | US6545127B1 (enExample) |
| EP (2) | EP1194449B1 (enExample) |
| JP (2) | JP5138851B2 (enExample) |
| CN (1) | CN1379819A (enExample) |
| AT (1) | ATE482233T1 (enExample) |
| AU (2) | AU5773500A (enExample) |
| CA (2) | CA2374346C (enExample) |
| DE (1) | DE60045005D1 (enExample) |
| WO (2) | WO2001000665A2 (enExample) |
Families Citing this family (114)
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| GB9701684D0 (en) | 1997-01-28 | 1997-03-19 | Smithkline Beecham Plc | Novel compounds |
| US6844148B1 (en) | 1998-09-24 | 2005-01-18 | Pharmacia & Upjohn Company | Alzheimer's disease secretase, APP substrates therefor, and uses therefor |
| US6699671B1 (en) * | 1998-09-24 | 2004-03-02 | Pharmacia & Upjohn Company | Alzheimer's disease secretase, APP substrates therefor, and uses therefor |
| US20040234976A1 (en) * | 1998-09-24 | 2004-11-25 | Gurney Mark E. | Alzheimer's disease secretase, app substrates therefor, and uses therefor |
| KR100839284B1 (ko) | 1998-09-24 | 2008-06-17 | 파마시아 앤드 업존 캄파니 엘엘씨 | 알츠하이머병 세크레타제 |
| US7115410B1 (en) | 1999-02-10 | 2006-10-03 | Elan Pharmaceuticals, Inc. | β-secretase enzyme compositions and methods |
| US7456007B1 (en) | 1998-12-31 | 2008-11-25 | Elan Pharmaceuticals, Inc. | β-secretase enzyme compositions and methods |
| CN1390232A (zh) | 1999-02-10 | 2003-01-08 | 艾兰制药公司 | 人β分泌酶、抑制剂、其组合物和用途 |
| JP5138851B2 (ja) * | 1999-06-28 | 2013-02-06 | オクラホマ メディカル リサーチ ファウンデーション | 触媒的に活性な組換えメマプシンおよびその使用方法 |
| US20090162883A1 (en) * | 1999-09-23 | 2009-06-25 | Pharmacia & Upjohn Company | Alzheimer's Disease Secretase, APP Substrates Thereof, and Uses Thereof |
| GB9924957D0 (en) * | 1999-10-21 | 1999-12-22 | Smithkline Beecham Plc | Novel treatment |
| GB9925136D0 (en) * | 1999-10-22 | 1999-12-22 | Smithkline Beecham Plc | Novel treatment |
| US7514408B1 (en) | 1999-12-02 | 2009-04-07 | Elan Pharmaceuticals, Inc. | β-secretase enzyme compositions and methods |
| EP1254108A1 (en) * | 2000-01-24 | 2002-11-06 | MERCK SHARP & DOHME LTD. | Gamma-secretase inhibitors |
| PT1255769E (pt) | 2000-01-25 | 2007-07-23 | Oklahoma Med Res Found | Procedimento universal para o re-enrolamento de proteínas recombinantes. |
| US7119085B2 (en) | 2000-03-23 | 2006-10-10 | Elan Pharmaceuticals, Inc. | Methods to treat alzheimer's disease |
| US6846813B2 (en) | 2000-06-30 | 2005-01-25 | Pharmacia & Upjohn Company | Compounds to treat alzheimer's disease |
| PE20020276A1 (es) | 2000-06-30 | 2002-04-06 | Elan Pharm Inc | COMPUESTOS DE AMINA SUSTITUIDA COMO INHIBIDORES DE ß-SECRETASA PARA EL TRATAMIENTO DE ALZHEIMER |
| EP1666452A2 (en) | 2000-06-30 | 2006-06-07 | Elan Pharmaceuticals, Inc. | Compounds to treat Alzheimer's disease |
| CA2410680A1 (en) | 2000-06-30 | 2002-01-10 | Elan Pharmaceuticals, Inc. | Compounds to treat alzheimer's disease |
| TW200628612A (en) * | 2000-07-19 | 2006-08-16 | Pharmacia & Up John Company | Substrates and assays for β-secretase activity |
| DE60126970T2 (de) * | 2000-09-22 | 2007-11-22 | Wyeth | Kristallstruktur von bace und verwendungen dafür |
| US7217556B1 (en) * | 2000-12-23 | 2007-05-15 | Pfizer Inc | Crystallization and structure determination of glycosylated human beta secretase, an enzyme implicated in Alzheimer's disease |
| US7601528B1 (en) | 2000-12-23 | 2009-10-13 | Elan Pharmaceuticals, Inc. | Crystallization and structure determination of glycosylated human beta secretase, an enzyme implicated in alzheimer's disease |
| US7806980B2 (en) | 2000-12-23 | 2010-10-05 | Elan Pharmaceuticals, Inc. | Method for crystallizing human beta secretase in complex with an inhibitor |
| US20040121947A1 (en) * | 2000-12-28 | 2004-06-24 | Oklahoma Medical Research Foundation | Compounds which inhibit beta-secretase activity and methods of use thereof |
| EP1233021A3 (en) * | 2001-02-20 | 2002-11-20 | Pfizer Products Inc. | An inhibitor of Beta amyloid cleavage enzyme |
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-
2000
- 2000-06-27 JP JP2001507069A patent/JP5138851B2/ja not_active Expired - Fee Related
- 2000-06-27 WO PCT/US2000/017742 patent/WO2001000665A2/en not_active Ceased
- 2000-06-27 JP JP2001507071A patent/JP4484410B2/ja not_active Expired - Fee Related
- 2000-06-27 WO PCT/US2000/017661 patent/WO2001000663A2/en not_active Ceased
- 2000-06-27 CN CN00810930A patent/CN1379819A/zh active Pending
- 2000-06-27 CA CA2374346A patent/CA2374346C/en not_active Expired - Fee Related
- 2000-06-27 DE DE60045005T patent/DE60045005D1/de not_active Expired - Lifetime
- 2000-06-27 US US09/604,608 patent/US6545127B1/en not_active Expired - Lifetime
- 2000-06-27 CA CA002374610A patent/CA2374610A1/en not_active Abandoned
- 2000-06-27 AU AU57735/00A patent/AU5773500A/en not_active Abandoned
- 2000-06-27 EP EP00943236A patent/EP1194449B1/en not_active Expired - Lifetime
- 2000-06-27 AU AU57715/00A patent/AU5771500A/en not_active Abandoned
- 2000-06-27 AT AT00943236T patent/ATE482233T1/de not_active IP Right Cessation
- 2000-06-27 EP EP00943208A patent/EP1196609A2/en not_active Withdrawn
-
2001
- 2001-02-28 US US09/795,903 patent/US20020164760A1/en not_active Abandoned
- 2001-04-30 US US09/845,226 patent/US20020115600A1/en not_active Abandoned
-
2004
- 2004-02-06 US US10/773,754 patent/US20040220079A1/en not_active Abandoned
- 2004-04-08 US US10/820,953 patent/US7244708B2/en not_active Expired - Fee Related
-
2007
- 2007-06-14 US US11/763,342 patent/US7678760B2/en not_active Expired - Fee Related
- 2007-08-03 US US11/888,920 patent/US7829669B2/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| US20040167075A1 (en) | 2004-08-26 |
| US20020115600A1 (en) | 2002-08-22 |
| EP1196609A2 (en) | 2002-04-17 |
| ATE482233T1 (de) | 2010-10-15 |
| US20080112946A1 (en) | 2008-05-15 |
| EP1194449B1 (en) | 2010-09-22 |
| US7244708B2 (en) | 2007-07-17 |
| US7829669B2 (en) | 2010-11-09 |
| EP1194449A2 (en) | 2002-04-10 |
| AU5773500A (en) | 2001-01-31 |
| CA2374346A1 (en) | 2001-01-04 |
| US20080021196A1 (en) | 2008-01-24 |
| US20020164760A1 (en) | 2002-11-07 |
| JP2003503072A (ja) | 2003-01-28 |
| JP2003506322A (ja) | 2003-02-18 |
| CA2374346C (en) | 2010-06-22 |
| US6545127B1 (en) | 2003-04-08 |
| JP4484410B2 (ja) | 2010-06-16 |
| US20040220079A1 (en) | 2004-11-04 |
| WO2001000663A2 (en) | 2001-01-04 |
| US7678760B2 (en) | 2010-03-16 |
| CN1379819A (zh) | 2002-11-13 |
| JP5138851B2 (ja) | 2013-02-06 |
| WO2001000665A2 (en) | 2001-01-04 |
| WO2001000663A3 (en) | 2001-10-04 |
| WO2001000665A9 (en) | 2002-07-25 |
| DE60045005D1 (de) | 2010-11-04 |
| AU5771500A (en) | 2001-01-31 |
| WO2001000665A3 (en) | 2001-09-27 |
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