CA2309386C - Variant loxp sequences and application of the same - Google Patents

Variant loxp sequences and application of the same Download PDF

Info

Publication number
CA2309386C
CA2309386C CA2309386A CA2309386A CA2309386C CA 2309386 C CA2309386 C CA 2309386C CA 2309386 A CA2309386 A CA 2309386A CA 2309386 A CA2309386 A CA 2309386A CA 2309386 C CA2309386 C CA 2309386C
Authority
CA
Canada
Prior art keywords
dna
gene
loxp site
mutant loxp
site
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CA2309386A
Other languages
English (en)
French (fr)
Other versions
CA2309386A1 (en
Inventor
Izumu Saito
Keiji Tanaka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Sumitomo Dainippon Pharma Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sumitomo Dainippon Pharma Co Ltd filed Critical Sumitomo Dainippon Pharma Co Ltd
Publication of CA2309386A1 publication Critical patent/CA2309386A1/en
Application granted granted Critical
Publication of CA2309386C publication Critical patent/CA2309386C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
CA2309386A 1997-11-13 1998-11-12 Variant loxp sequences and application of the same Expired - Fee Related CA2309386C (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
JP33128997 1997-11-13
JP9/331289 1997-11-13
JP10/273150 1998-09-28
JP27315098A JP4206154B2 (ja) 1997-11-13 1998-09-28 変異型ｌｏｘＰ配列とその応用
PCT/JP1998/005094 WO1999025851A1 (en) 1997-11-13 1998-11-12 VARIANT loxP SEQUENCES AND APPLICATION OF THE SAME

Publications (2)

Publication Number Publication Date
CA2309386A1 CA2309386A1 (en) 1999-05-27
CA2309386C true CA2309386C (en) 2010-07-13

Family

ID=26550537

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2309386A Expired - Fee Related CA2309386C (en) 1997-11-13 1998-11-12 Variant loxp sequences and application of the same

Country Status (12)

Country Link
US (1) US6465254B1 (https=)
EP (1) EP1035208B1 (https=)
JP (1) JP4206154B2 (https=)
KR (1) KR100639088B1 (https=)
CN (1) CN1173039C (https=)
AT (1) ATE412759T1 (https=)
AU (1) AU741415B2 (https=)
CA (1) CA2309386C (https=)
DE (1) DE69840173D1 (https=)
DK (1) DK1035208T3 (https=)
NZ (1) NZ504307A (https=)
WO (1) WO1999025851A1 (https=)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6964861B1 (en) 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
DK0937098T3 (da) 1995-06-07 2002-12-02 Invitrogen Corp Rekombinatorisk kloning under anvendelse af gensplejsede rekombinationssites
US6143557A (en) 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US5851808A (en) 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US7351578B2 (en) 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
EP1025217B1 (en) 1997-10-24 2006-10-04 Invitrogen Corporation Recombinational cloning using nucleic acids having recombination sites
JP4153607B2 (ja) 1998-11-18 2008-09-24 大日本住友製薬株式会社 新規な組換えアデノウイルス
EP2119788A1 (en) 1999-03-02 2009-11-18 Life Technologies Corporation Compositions and methods for use in recombinational cloning of nucleic acids
AU6529900A (en) * 1999-08-09 2001-03-05 Monsanto Technology Llc Novel cloning methods and vectors
EP2210948A3 (en) 1999-12-10 2010-10-06 Life Technologies Corporation Use of multiple recombination sites with unique specificity in recombinational cloning
JP2001321174A (ja) * 2000-05-11 2001-11-20 Japan Science & Technology Corp アンチセンス型ジーントラップベクター
US7244560B2 (en) 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
EP1322152A2 (en) * 2000-10-03 2003-07-02 ADEREGEM (Association pour le Développement de la Recherche en Génétique Moléculaire Transgenic mouse for targeted recombination mediated by modified cre-er
FR2814642B1 (fr) * 2000-10-03 2005-07-01 Ass Pour Le Dev De La Rech En Souris transgenique pour la recombinaison ciblee mediee par la cre-er modifiee
WO2002083889A2 (en) * 2001-04-10 2002-10-24 Imperial College Innovations Limited Methods for the production of minicircles
EP1427816A4 (en) * 2001-09-18 2004-12-01 Clontech Lab Inc METHOD FOR THE PRODUCTION OF ADENOVIRUS VECTORS, BASED ON A SPECIFIC RECOMBINASE
EP1457559B1 (en) 2001-11-22 2008-01-16 Keio University Artificial antibody library with super-repertory
US6818420B2 (en) 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
CA2484360A1 (en) 2002-05-02 2003-11-13 The University Of North Carolina At Chapel Hill In vitro mutagenesis, phenotyping, and gene mapping
WO2004020605A2 (en) 2002-08-29 2004-03-11 The Board Of Trustees Of The Leland Stanford Junior University Circular nucleic acid vectors, and methods for making and using the same
WO2004078939A2 (en) * 2003-03-03 2004-09-16 University Of Massachusetts REGULATION OF Mdm2 FUNCTION
WO2005042781A2 (en) 2003-10-31 2005-05-12 Agencourt Personal Genomics Corporation Methods for producing a paired tag from a nucleic acid sequence and methods of use thereof
US8304189B2 (en) 2003-12-01 2012-11-06 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same
WO2005081632A2 (en) * 2004-02-26 2005-09-09 State Of Israel, Ministry Of Agriculture, Agricultural Research Organization Enzymes, cells and methods for site specific recombination at asymmetric sites
JP2006333742A (ja) * 2005-05-31 2006-12-14 Chemicals Evaluation & Research Institute 細胞及び細胞へのｄｎａ導入方法
BRPI0612862A2 (pt) 2005-07-18 2010-11-30 Pioneer Hi Bred Int polinucleotìdeo isolado, método para obtenção de uma célula transformada, método para obtenção de uma planta transformada, método de produção de recombinação de dna de sìtio especìfico, biblioteca isolada, método de geração de biblioteca de moléculas e método para selecionar sìtio de recombinação frt modificado
JP2009524406A (ja) * 2005-10-13 2009-07-02 ビーシー キャンサー エージェンシー 合成生物学および代謝工学のためのモジュラー型ゲノム
US20070143881A1 (en) * 2005-12-16 2007-06-21 Pioneer Hi-Bred International, Inc. Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange
US7993913B2 (en) 2006-05-09 2011-08-09 The Curators Of The University Of Missouri Plant artificial chromosome platforms via telomere truncation
WO2007137114A2 (en) 2006-05-17 2007-11-29 Pioneer Hi-Bred International, Inc. Artificial plant minichromosomes
CN101849010A (zh) 2007-06-29 2010-09-29 先锋高级育种国际公司 用于改变单子叶植物细胞的基因组的方法
EP2067858A1 (en) 2007-12-07 2009-06-10 Universidad de Sevilla Animal models for neurodegenerative diseases
WO2012044847A1 (en) 2010-10-01 2012-04-05 Life Technologies Corporation Nucleic acid adaptors and uses thereof
EP2235217B1 (en) 2008-01-09 2016-04-20 Life Technologies Corporation Method of making a paired tag library for nucleic acid sequencing
ES2337973B8 (es) 2008-05-16 2011-07-21 Proyecto De Biomedicina Cima, S.L. Adenovirus auxiliares auto-inactivantes para la produccion de adenovirus recombinantes de alta capacidad.
US8704041B2 (en) 2009-12-30 2014-04-22 Pioneer Hi Bred International Inc Methods and compositions for targeted polynucleotide modification
MX2013010911A (es) 2011-03-23 2015-03-03 Pioneer Hi Bred Int Metodos para producir un locus de rasgo transgenico complejo.
KR102068107B1 (ko) 2011-04-27 2020-01-20 아미리스 인코퍼레이티드 게놈 변형 방법
BR112013033176A2 (pt) 2011-06-21 2018-06-12 E I Du Pont De Nemouras And Company método para produzir uma modificação direcionada em um gene de fertilidade masculina, planta, molécula de ácido nucleio isolada, construto de expressão
EP2825651B1 (en) * 2012-03-12 2018-04-25 DSM IP Assets B.V. Recombination system
CN103361342B (zh) * 2012-03-30 2019-01-25 上海杰隆生物工程股份有限公司 一种转基因定位整合的方法及其应用
US20140173781A1 (en) 2012-12-13 2014-06-19 Pioneer Hi-Bred International, Inc. Methods and compositions for producing and selecting transgenic wheat plants
ES2523016B1 (es) 2013-05-20 2015-09-09 3P Biopharmaceuticals Vectores alfavirales y líneas celulares para la producción de proteínas recombinantes
MX2016007797A (es) 2013-12-19 2016-09-07 Amyris Inc Metodos para integracion genomica.
JP2018501814A (ja) 2014-11-11 2018-01-25 クララ フーズ カンパニー 卵白タンパク質産生のための方法および組成物
CN105316362B (zh) * 2015-08-19 2020-03-17 暨南大学 一种Dual-RMCE介导的TCR基因置换系统及其方法
CN108513584A (zh) 2015-08-28 2018-09-07 先锋国际良种公司 苍白杆菌介导的植物转化
EP4219731A3 (en) 2016-05-18 2023-08-09 Amyris, Inc. Compositions and methods for genomic integration of nucleic acids into exogenous landing pads
BR112022000545A2 (pt) 2019-07-11 2022-04-05 Clara Foods Co Composições de proteína e produtos de consumo destas
US12096784B2 (en) 2019-07-11 2024-09-24 Clara Foods Co. Protein compositions and consumable products thereof
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
WO2023015822A1 (zh) * 2021-08-13 2023-02-16 上海鑫湾生物科技有限公司 缺氧触发的人工转录因子、转录控制系统及其应用
WO2023127871A1 (ja) 2021-12-27 2023-07-06 株式会社レプリテック 温度感受性のマイナス鎖ｒｎａウイルスまたはウイルスベクターおよびそのｒｎａゲノム
CN117165627A (zh) * 2022-05-27 2023-12-05 上海科技大学 基于Cre-LoxP和CRISPR的核酸构建体及其应用
JPWO2024195811A1 (https=) 2023-03-22 2024-09-26
CN118147146B (zh) * 2024-03-25 2024-09-17 中国科学院水生生物研究所 突变型LoxP元件、其表达载体和在鱼类基因工程育种中的应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6228646B1 (en) * 1996-03-07 2001-05-08 The Regents Of The University Of California Helper-free, totally defective adenovirus for gene therapy
EP0941310A1 (en) * 1996-10-11 1999-09-15 THE TEXAS A&M UNIVERSITY SYSTEM Methods for the generation of primordial germ cells and transgenic animal species

Also Published As

Publication number Publication date
US6465254B1 (en) 2002-10-15
KR20010032020A (ko) 2001-04-16
ATE412759T1 (de) 2008-11-15
JPH11196880A (ja) 1999-07-27
CA2309386A1 (en) 1999-05-27
CN1283233A (zh) 2001-02-07
EP1035208A4 (en) 2002-01-30
KR100639088B1 (ko) 2006-10-27
NZ504307A (en) 2001-07-27
DK1035208T3 (da) 2009-02-23
JP4206154B2 (ja) 2009-01-07
WO1999025851A1 (en) 1999-05-27
DE69840173D1 (de) 2008-12-11
EP1035208A1 (en) 2000-09-13
CN1173039C (zh) 2004-10-27
AU1052699A (en) 1999-06-07
EP1035208B1 (en) 2008-10-29
AU741415B2 (en) 2001-11-29

Similar Documents

Publication Publication Date Title
CA2309386C (en) Variant loxp sequences and application of the same
US6340595B1 (en) High throughput screening of gene function using adenoviral libraries for functional genomics applications
US5817492A (en) Recombinant DNA viral vector for transfecting animal cells
US5700470A (en) Recombinant adenovirus with removed EZA gene and method of preparation
US6228646B1 (en) Helper-free, totally defective adenovirus for gene therapy
JP4787440B2 (ja) 変異型ｆｒｔ配列を含有してなるｄｎａ
JP4597188B2 (ja) 多タンパク質適用のための新規発現ツール
JP4493492B2 (ja) 脊椎動物における遺伝子移入のためのトランスポゾンベクターであるＦｒｏｇＰｒｉｎｃｅ
FR2727689A1 (fr) Nouveau procede de preparation d'un vecteur viral
JPWO2001023545A1 (ja) 変異型ｆｒｔ配列を含有してなるｄｎａ
US6806080B2 (en) Hybrid vectors for gene therapy
US7083971B1 (en) Hybrid yeast-bacteria cloning system and uses thereof
JP4159620B2 (ja) 組換えアデノウイルスの製造方法
JP3713038B2 (ja) 組換えアデノウイルス
JPH104973A (ja) 組換えバキュロウイルス及びその利用

Legal Events

Date Code Title Description
EEER Examination request
MKLA Lapsed

Effective date: 20151112