DE69840173D1 - LoxP SEQUENZ-VARIANTEN UND DEREN VERWENDUNG - Google Patents

LoxP SEQUENZ-VARIANTEN UND DEREN VERWENDUNG

Info

Publication number
DE69840173D1
DE69840173D1 DE69840173T DE69840173T DE69840173D1 DE 69840173 D1 DE69840173 D1 DE 69840173D1 DE 69840173 T DE69840173 T DE 69840173T DE 69840173 T DE69840173 T DE 69840173T DE 69840173 D1 DE69840173 D1 DE 69840173D1
Authority
DE
Germany
Prior art keywords
bases
loxp site
wild
image
specific recombination
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69840173T
Other languages
English (en)
Inventor
Izumu Saito
Keiji Tanaka
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Sumitomo Pharma Co Ltd
Original Assignee
Sumitomo Dainippon Pharma Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sumitomo Dainippon Pharma Co Ltd filed Critical Sumitomo Dainippon Pharma Co Ltd
Application granted granted Critical
Publication of DE69840173D1 publication Critical patent/DE69840173D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Mycology (AREA)
  • Virology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Polysaccharides And Polysaccharide Derivatives (AREA)
  • Detergent Compositions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69840173T 1997-11-13 1998-11-12 LoxP SEQUENZ-VARIANTEN UND DEREN VERWENDUNG Expired - Lifetime DE69840173D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP33128997 1997-11-13
JP27315098A JP4206154B2 (ja) 1997-11-13 1998-09-28 変異型ｌｏｘＰ配列とその応用
PCT/JP1998/005094 WO1999025851A1 (fr) 1997-11-13 1998-11-12 Sequences 1oxp de variant et leur application

Publications (1)

Publication Number Publication Date
DE69840173D1 true DE69840173D1 (de) 2008-12-11

Family

ID=26550537

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69840173T Expired - Lifetime DE69840173D1 (de) 1997-11-13 1998-11-12 LoxP SEQUENZ-VARIANTEN UND DEREN VERWENDUNG

Country Status (12)

Country Link
US (1) US6465254B1 (de)
EP (1) EP1035208B1 (de)
JP (1) JP4206154B2 (de)
KR (1) KR100639088B1 (de)
CN (1) CN1173039C (de)
AT (1) ATE412759T1 (de)
AU (1) AU741415B2 (de)
CA (1) CA2309386C (de)
DE (1) DE69840173D1 (de)
DK (1) DK1035208T3 (de)
NZ (1) NZ504307A (de)
WO (1) WO1999025851A1 (de)

Families Citing this family (50)

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US6964861B1 (en) 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
AU724922B2 (en) 1995-06-07 2000-10-05 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US5851808A (en) 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
CA2307016A1 (en) 1997-10-24 1999-05-06 Life Technologies, Inc. Recombinational cloning using nucleic acids having recombination sites
JP4153607B2 (ja) 1998-11-18 2008-09-24 大日本住友製薬株式会社 新規な組換えアデノウイルス
AU774643B2 (en) 1999-03-02 2004-07-01 Invitrogen Corporation Compositions and methods for use in recombinational cloning of nucleic acids
WO2001011058A1 (en) * 1999-08-09 2001-02-15 Monsanto Technology Llc Novel cloning methods and vectors
JP2001321174A (ja) * 2000-05-11 2001-11-20 Japan Science & Technology Corp アンチセンス型ジーントラップベクター
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
WO2002028175A2 (en) * 2000-10-03 2002-04-11 Association Pour Le Developpement De La Recherche En Genetique Moleculaire (Aderegem) Transgenic mouse for targeted recombination mediated by modified cre-er
FR2814642B1 (fr) * 2000-10-03 2005-07-01 Ass Pour Le Dev De La Rech En Souris transgenique pour la recombinaison ciblee mediee par la cre-er modifiee
WO2002083889A2 (en) * 2001-04-10 2002-10-24 Imperial College Innovations Limited Methods for the production of minicircles
US6838285B2 (en) * 2001-09-18 2005-01-04 Becton Dickinson Site specific recombinase based method for producing adenoviral vectors
US8178320B2 (en) 2001-11-22 2012-05-15 Keio University Artificial antibody library with super-repertory
US6818420B2 (en) 2002-02-27 2004-11-16 Biosource International, Inc. Methods of using FET labeled oligonucleotides that include a 3′-5′ exonuclease resistant quencher domain and compositions for practicing the same
IL164687A0 (en) 2002-05-02 2005-12-18 Univ North Carolina Cellular libraries and methods for the preparationthereof
IL166612A0 (en) * 2002-08-29 2006-01-15 Univ Leland Stanford Junior Circular nucleic acid vectors and methods for making and using the same
US20040216178A1 (en) * 2003-03-03 2004-10-28 Jones Stephen N Regulation of mdm2 function
EP2202322A1 (de) 2003-10-31 2010-06-30 AB Advanced Genetic Analysis Corporation Verfahren zur Herstellung eines gepaarten Markers aus einer Nukleinsäuresequenz und Verwendungsverfahren dafür
ATE469984T1 (de) 2003-12-01 2010-06-15 Life Technologies Corp Rekombinationsstellen enthaltende nukleinsäuremoleküle und verfahren zur verwendung davon
WO2005081632A2 (en) * 2004-02-26 2005-09-09 State Of Israel, Ministry Of Agriculture, Agricultural Research Organization Enzymes, cells and methods for site specific recombination at asymmetric sites
JP2006333742A (ja) * 2005-05-31 2006-12-14 Chemicals Evaluation & Research Institute 細胞及び細胞へのｄｎａ導入方法
MX2008000764A (es) * 2005-07-18 2008-03-24 Pioneer Hi Bred Int Sitios de recombinacion frt modificados y metodos de uso.
EP1948803A4 (de) * 2005-10-13 2009-11-25 Bc Cancer Agency Modulare genome für synthetische biologie und stoffwechselmanipulation
US20070143881A1 (en) * 2005-12-16 2007-06-21 Pioneer Hi-Bred International, Inc. Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange
CA2651895C (en) 2006-05-09 2014-05-06 The Curators Of The University Of Missouri Plant artificial chromosome platforms via telomere truncation
BRPI0712398A2 (pt) 2006-05-17 2012-10-16 Pioneer Hi-Bred International minicromossomo artificial de planta, planta de milho, polinucleotìdeo isolado, construção recombinante, planta transgênica de milho e método para produzir uma planta transgênica de milho compreendendo um minicromossomo artificial de planta tendo centrÈmero funcional
EP2167666A2 (de) 2007-06-29 2010-03-31 Pioneer Hi-Bred International Inc. Verfahren zur veränderung des genoms einer monokotyledonen-zelle
EP2067858A1 (de) 2007-12-07 2009-06-10 Universidad de Sevilla Tiermodelle für neurodegenerative Erkrankungen
CN102016068A (zh) 2008-01-09 2011-04-13 生命科技公司 制备用于核酸测序的配对标签文库的方法
US9540637B2 (en) 2008-01-09 2017-01-10 Life Technologies Corporation Nucleic acid adaptors and uses thereof
ES2337973B8 (es) 2008-05-16 2011-07-21 Proyecto De Biomedicina Cima, S.L. Adenovirus auxiliares auto-inactivantes para la produccion de adenovirus recombinantes de alta capacidad.
EP2529017A2 (de) 2009-12-30 2012-12-05 Pioneer Hi-Bred International, Inc. Verfahren und zusammensetzungen für gezielte polynukleotidmodifikation
EA201391373A1 (ru) 2011-03-23 2014-07-30 Пайонир Хай-Бред Интернэшнл, Инк. Способы получения сложного локуса трансгенных признаков
CA2834375C (en) 2011-04-27 2020-07-14 Amyris, Inc. Methods for genomic modification
CA2844470A1 (en) 2011-06-21 2013-05-10 Pioneer Hi-Bred International, Inc. Methods and compositions for producing male sterile plants
WO2013135729A1 (en) * 2012-03-12 2013-09-19 Dsm Ip Assets B.V. Recombination system
CN103361342B (zh) * 2012-03-30 2019-01-25 上海杰隆生物工程股份有限公司 一种转基因定位整合的方法及其应用
US20140173775A1 (en) 2012-12-13 2014-06-19 Pioneer Hi-Bred International, Inc. Methods and compositions for producing and selecting transgenic plants
ES2523016B1 (es) 2013-05-20 2015-09-09 3P Biopharmaceuticals Vectores alfavirales y líneas celulares para la producción de proteínas recombinantes
JP6652489B2 (ja) 2013-12-19 2020-02-26 アミリス， インコーポレイテッド ゲノム組込みのための方法
JP2018501814A (ja) 2014-11-11 2018-01-25 クララ フーズ カンパニー 卵白タンパク質産生のための方法および組成物
CN105316362B (zh) * 2015-08-19 2020-03-17 暨南大学 一种Dual-RMCE介导的TCR基因置换系统及其方法
AU2016315655A1 (en) 2015-08-28 2018-02-01 E. I. Du Pont De Nemours And Company Ochrobactrum-mediated transformation of plants
EP3458595A2 (de) 2016-05-18 2019-03-27 Amyris, Inc. Zusammensetzungen und verfahren für genomische integration von nukleinsäuren in exogene landing-pads
EP3997118A4 (de) 2019-07-11 2023-07-19 Clara Foods Co. Proteinzusammensetzungen und verbrauchbare produkte daraus
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
AU2021459965A1 (en) * 2021-08-13 2024-02-22 Shanghai Sinobay Biotechnology Co., Ltd. A Hypoxia-triggered Artificial Transcription Factor, A Hypoxia-triggered Transcription Control System and Use Thereof
CN117165627A (zh) * 2022-05-27 2023-12-05 上海科技大学 基于Cre-LoxP和CRISPR的核酸构建体及其应用

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US6228646B1 (en) * 1996-03-07 2001-05-08 The Regents Of The University Of California Helper-free, totally defective adenovirus for gene therapy
JP2002511732A (ja) * 1996-10-11 2002-04-16 ザ・テキサス・エイ・アンド・エム・ユニヴァーシティ・システム トランスジェニック動物を発生させるための組成物および方法

Also Published As

Publication number Publication date
JP4206154B2 (ja) 2009-01-07
JPH11196880A (ja) 1999-07-27
CA2309386C (en) 2010-07-13
AU1052699A (en) 1999-06-07
ATE412759T1 (de) 2008-11-15
CN1283233A (zh) 2001-02-07
DK1035208T3 (da) 2009-02-23
EP1035208A1 (de) 2000-09-13
AU741415B2 (en) 2001-11-29
CA2309386A1 (en) 1999-05-27
NZ504307A (en) 2001-07-27
WO1999025851A1 (fr) 1999-05-27
KR100639088B1 (ko) 2006-10-27
CN1173039C (zh) 2004-10-27
EP1035208A4 (de) 2002-01-30
KR20010032020A (ko) 2001-04-16
US6465254B1 (en) 2002-10-15
EP1035208B1 (de) 2008-10-29

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