CA2308762A1 - Specific and sensitive nucleic acid detection method - Google Patents

Specific and sensitive nucleic acid detection method Download PDF

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Publication number
CA2308762A1
CA2308762A1 CA002308762A CA2308762A CA2308762A1 CA 2308762 A1 CA2308762 A1 CA 2308762A1 CA 002308762 A CA002308762 A CA 002308762A CA 2308762 A CA2308762 A CA 2308762A CA 2308762 A1 CA2308762 A1 CA 2308762A1
Authority
CA
Canada
Prior art keywords
nucleic acid
sequence
probe
detected
binding
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002308762A
Other languages
English (en)
French (fr)
Inventor
Christoph Kessler
Gerd Haberhausen
Knut Bartl
Henrik Orum
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19748690A external-priority patent/DE19748690A1/de
Priority claimed from DE19814001A external-priority patent/DE19814001A1/de
Priority claimed from DE19814828A external-priority patent/DE19814828A1/de
Application filed by Individual filed Critical Individual
Publication of CA2308762A1 publication Critical patent/CA2308762A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CA002308762A 1997-11-04 1998-11-03 Specific and sensitive nucleic acid detection method Abandoned CA2308762A1 (en)

Applications Claiming Priority (7)

Application Number Priority Date Filing Date Title
DE19748690A DE19748690A1 (de) 1997-11-04 1997-11-04 Spezifisches und sensitives Nukleinsäurenachweisverfahren
DE19748690.8 1997-11-04
DE19814001.0 1998-03-28
DE19814001A DE19814001A1 (de) 1998-03-28 1998-03-28 Spezifisches und sensitives Nukleinsäurenachweisverfahren
DE19814828A DE19814828A1 (de) 1998-04-02 1998-04-02 Spezifisches und sensitives Nukleinsäurenachweisverfahren
DE19814828.3 1998-04-02
PCT/EP1998/006961 WO1999023250A2 (de) 1997-11-04 1998-11-03 Spezifisches und sensitives nukleinsäurenachweisverfahren

Publications (1)

Publication Number Publication Date
CA2308762A1 true CA2308762A1 (en) 1999-05-14

Family

ID=27217887

Family Applications (3)

Application Number Title Priority Date Filing Date
CA002308762A Abandoned CA2308762A1 (en) 1997-11-04 1998-11-03 Specific and sensitive nucleic acid detection method
CA002308368A Expired - Fee Related CA2308368C (en) 1997-11-04 1998-11-03 Specific and sensitive nucleic acid detection method
CA002312779A Abandoned CA2312779A1 (en) 1997-11-04 1998-11-03 Specific and sensitive nucleic acid detection method

Family Applications After (2)

Application Number Title Priority Date Filing Date
CA002308368A Expired - Fee Related CA2308368C (en) 1997-11-04 1998-11-03 Specific and sensitive nucleic acid detection method
CA002312779A Abandoned CA2312779A1 (en) 1997-11-04 1998-11-03 Specific and sensitive nucleic acid detection method

Country Status (6)

Country Link
US (1) US7105318B2 (https=)
EP (3) EP1029083A2 (https=)
JP (3) JP2002505071A (https=)
AU (3) AU2152099A (https=)
CA (3) CA2308762A1 (https=)
WO (3) WO1999023250A2 (https=)

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7569342B2 (en) 1997-12-10 2009-08-04 Sierra Molecular Corp. Removal of molecular assay interferences
CA2313641A1 (en) * 1997-12-12 1999-06-24 Digene Corporation Universal collection medium
WO1999049293A2 (en) 1998-03-24 1999-09-30 Boston Probes, Inc. Methods, kits and compositions pertaining to detection complexes
US7439016B1 (en) * 2000-06-15 2008-10-21 Digene Corporation Detection of nucleic acids by type-specific hybrid capture method
US7601497B2 (en) * 2000-06-15 2009-10-13 Qiagen Gaithersburg, Inc. Detection of nucleic acids by target-specific hybrid capture method
US7807802B2 (en) 2002-11-12 2010-10-05 Abbott Lab Polynucleotides for the amplification and detection of Chlamydia trachomatis and Neisseria gonorrhoeae
EP3095873B1 (en) 2006-12-21 2018-04-18 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
US8617816B2 (en) * 2007-03-16 2013-12-31 454 Life Sciences, A Roche Company System and method for detection of HIV drug resistant variants
GB0714389D0 (en) 2007-07-21 2007-09-05 Barry Callebaut Ag Process and product
CA2726396C (en) * 2008-04-17 2019-03-19 Qiagen Gaithersburg, Inc. Compositions, methods, and kits using synthetic probes for determining the presence of a target nucleic acid
TWI419974B (zh) 2008-10-27 2013-12-21 Qiagen Gaithersburg Inc 於自動平台上之快速結果雜交捕捉法
ES2644516T3 (es) * 2009-01-28 2017-11-29 Qiagen Gaithersburg, Inc. Método y ensayo de preparación de muestras de gran volumen específico de secuencia
WO2010127228A1 (en) * 2009-05-01 2010-11-04 Qiagen Gaithersburg, Inc. A non-target amplification method for detection of rna splice-forms in a sample
US8512955B2 (en) 2009-07-01 2013-08-20 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
JP5826752B2 (ja) 2009-09-14 2015-12-02 キアジェン ゲイサーズバーグ インコーポレイテッド 細胞学用培地に固定された組織サンプルから核酸またはタンパク質を回収するための組成物および方法
CA2787924A1 (en) 2010-01-29 2011-08-04 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
JP2013517803A (ja) 2010-01-29 2013-05-20 キアジェン ゲイサーズバーグ インコーポレイテッド サンプル中のｈｐｖの存在を決定および確認する方法
EP2572001A2 (en) 2010-05-19 2013-03-27 QIAGEN Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
JP6153866B2 (ja) 2010-05-25 2017-06-28 キアゲン ガイサーズバーグ アイエヌシー． 迅速なハイブリッド捕捉アッセイ、及び関連する戦略的に切断されたプローブ
WO2012116220A2 (en) 2011-02-24 2012-08-30 Qiagen Gaithersburg, Inc. Materials and methods for detection of hpv nucleic acid
JP2024097096A (ja) * 2021-04-28 2024-07-18 バイオニクス株式会社 光学測定装置

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1190838A (en) * 1981-07-17 1985-07-23 Cavit Akin Homogeneous nucleic acid hybridization diagnostics by non-radiative energy transfer
US5200313A (en) 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
AU606043B2 (en) * 1985-03-28 1991-01-31 F. Hoffmann-La Roche Ag Detection of viruses by amplification and hybridization
ES286841Y (es) 1985-05-20 1988-10-01 Vileda Gmbh Dispositivo de acoplamiento de un mango a un util de limpieza
CA1340807C (en) 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5639871A (en) * 1988-09-09 1997-06-17 Roche Molecular Systems, Inc. Detection of human papillomavirus by the polymerase chain reaction
WO1991001384A1 (en) 1989-07-11 1991-02-07 Gen-Probe, Incorporated Nucleic acid sequence amplification methods utilizing a transcription complex
NL9000134A (nl) * 1990-01-19 1991-08-16 Stichting Res Fonds Pathologie Primers en werkwijze voor het detecteren van humaan papilloma virus genotypen m.b.v. pcr.
US5453355A (en) * 1990-01-26 1995-09-26 Abbott Laboratories Oligonucleotides and methods for the detection of Neisseria gonorrhoeae
US5210015A (en) 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
PL169880B1 (pl) * 1991-05-08 1996-09-30 Chiron Corp Sposób wytwarzania produktu hybrydyzacji z kwasem nukleinowym wirusa zapalenia watroby C PL PL PL
ES2111589T3 (es) 1991-08-27 1998-03-16 Hoffmann La Roche Metodos y reactivos para la deteccion de la hepatitis c.
JPH05308999A (ja) * 1992-05-08 1993-11-22 Sumitomo Metal Ind Ltd Ｂ型肝炎ウイルスｐｒｅ−Ｃ変異の判定法
WO1994003635A1 (en) 1992-08-04 1994-02-17 Institute Of Molecular Biology & Biotechnology Rapid amplification and detection of nucleic acids
US5605798A (en) * 1993-01-07 1997-02-25 Sequenom, Inc. DNA diagnostic based on mass spectrometry
EP0785996A1 (en) * 1993-07-13 1997-07-30 Abbott Laboratories Nucleotide sequences and process for amplifying and detection of hepatitis b virus
US5538848A (en) * 1994-11-16 1996-07-23 Applied Biosystems Division, Perkin-Elmer Corp. Method for detecting nucleic acid amplification using self-quenching fluorescence probe
AT406019B (de) * 1995-05-08 2000-01-25 Immuno Ag Verfahren zur herstellung eines arzneimittels enthaltend ein oder mehrere plasmaderivate
US5882857A (en) 1995-06-07 1999-03-16 Behringwerke Ag Internal positive controls for nucleic acid amplification
JP4540754B2 (ja) 1996-06-04 2010-09-08 ユニバーシティ オブ ユタ リサーチ ファウンデーション Ｐｃｒ中のハイブリダイゼーションのモニタリング
US5858671A (en) 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6333179B1 (en) * 1997-06-20 2001-12-25 Affymetrix, Inc. Methods and compositions for multiplex amplification of nucleic acids
CA2318880C (en) 1998-02-04 2010-07-27 Applied Biosystems, Llc Determination of a genotype of an amplification product at multiple allelic sites
US5948648A (en) 1998-05-29 1999-09-07 Khan; Shaheer H. Nucleotide compounds including a rigid linker

Also Published As

Publication number Publication date
EP1029077A2 (de) 2000-08-23
WO1999024606A3 (de) 1999-07-22
US20030175765A1 (en) 2003-09-18
US7105318B2 (en) 2006-09-12
WO1999023249A3 (de) 1999-09-10
AU741141B2 (en) 2001-11-22
AU2152199A (en) 1999-05-24
WO1999023249A2 (de) 1999-05-14
WO1999023250A3 (de) 1999-07-22
AU2152099A (en) 1999-05-31
WO1999023250A2 (de) 1999-05-14
AU1232099A (en) 1999-05-24
CA2308368A1 (en) 1999-05-14
EP1029084A2 (de) 2000-08-23
EP1029083A2 (de) 2000-08-23
JP2001521765A (ja) 2001-11-13
CA2312779A1 (en) 1999-05-20
WO1999024606A2 (de) 1999-05-20
JP2002505071A (ja) 2002-02-19
JP2002509694A (ja) 2002-04-02
CA2308368C (en) 2009-01-20

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Legal Events

Date Code Title Description
EEER Examination request
FZDE Discontinued